Standard name
Description Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; RSC3 has a paralog, RSC30, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.95 0.98 0.99 0.98 0.96 0.94 0.95 0.87 0.87 0.75 0.78 0.99 0.99 0.99 0.94 0.93 0.81 0.77 0.74 0.69 0.64 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.07 0.25 0.26 0.37 0.27 0 0 0 0.06 0 0.06 0.1 0.12 0.16 0.16 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 3 2 3 8 10 8
Bud 0 2 0 0 1 0 1 1 4 4 8 1 1 0 0 0 0 2 3 2 2 9 18
Bud Neck 1 0 10 8 13 1 16 13 6 8 6 13 0 0 0 0 0 1 0 1 3 5 5
Bud Site 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 0 0
Cell Periphery 0 2 4 5 1 0 1 0 1 2 0 0 0 0 0 3 0 0 1 4 5 7 6
Cytoplasm 247 239 299 270 285 245 424 382 279 275 140 181 381 478 472 105 115 209 201 208 252 269 285
Endoplasmic Reticulum 1 2 1 1 1 0 0 0 0 0 0 0 1 0 2 10 9 3 0 0 4 3 5
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 4 8 2 2 3 1
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 1 2 0 1 1 1
Mitochondria 4 10 0 1 0 11 31 27 80 81 68 63 3 2 1 7 3 16 25 33 58 67 59
Nucleus 0 0 2 1 2 0 2 8 2 4 0 6 0 1 2 0 2 0 0 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 2 1 1 4 2 7 2 6 7 0 1 1 0 1 3 2 7 2 9 6
Vac/Vac Membrane 0 3 1 0 0 4 6 2 0 3 0 1 0 1 0 4 7 5 5 4 14 16 13
Unique Cell Count 251 252 305 274 290 254 450 403 322 317 186 233 384 481 477 112 123 257 263 283 369 420 422
Labelled Cell Count 253 259 321 288 304 263 487 435 379 379 229 276 387 483 479 130 138 257 263 283 369 420 422


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.9 4.9 4.4 4.7 4.9 3.7 4.2 3.1 3.2 3.0 3.5 3.6 3.8 3.8 6.0 5.9 3.6 3.6 4.0
Std Deviation (1e-4) 0.9 1.0 2.6 2.4 1.6 5.4 1.4 2.0 1.4 1.4 1.5 1.2 1.1 1.3 0.9 1.9 2.1 1.4 1.6 2.0
Intensity Change (Log2) -0.17 -0.08 -0.0 -0.43 -0.24 -0.65 -0.63 -0.73 -0.5 -0.46 -0.37 -0.38 0.28 0.26 -0.45 -0.44 -0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available