Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the ER; catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER; CPR5 has a paralog, CPR2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0 0.19 0.37 0.31 0.3 0.39 – 0.45 0.34 0.52 0.47 0.39 0.55 0.68 0.23 0.17 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 – 0 0 0 0 0.12 0.07 0.06 0 0 0 0.05 0 0.05 0.1 0.06 0.1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.32 0 0 0 0.14 0 – 0.09 0.1 0.08 0.09 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Nucleus 0.17 0.18 0.43 0.34 0.21 0.24 0.24 – 0.24 0.22 0.14 0.14 0 0.05 0 0.4 0.31 0.39 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.74 0.76 0.53 0.52 0.71 0.58 0.57 – 0.51 0.56 0.52 0.53 0.61 0.53 0.39 0.47 0.58 0.53 0.82 0.86 0.78 0.8 0.82 0.74
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 – 0 1 1 0 1 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 1 2 2 – 1 1 3 2 1 1 0 0 0 0 0 0 0 2 3 2
Bud Neck 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 – 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0
Cytoplasm 14 1 9 60 86 78 185 – 128 121 148 196 73 156 254 31 20 18 2 1 4 1 7 4
Endoplasmic Reticulum 1 0 0 0 3 3 0 – 3 3 1 1 4 4 1 3 9 4 1 1 0 1 3 2
Endosome 8 0 0 1 8 0 3 – 1 1 0 1 22 20 21 5 5 4 8 2 4 17 21 22
Golgi 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 1 0 0 3 0 1 0 0 2
Mitochondria 11 12 1 6 2 37 9 – 27 34 24 36 2 2 0 6 2 5 2 0 1 1 5 4
Nucleus 25 7 20 54 58 62 116 – 69 79 41 58 8 14 10 53 38 38 4 2 4 3 3 5
Nuclear Periphery 1 0 0 1 0 0 1 – 1 2 0 4 0 1 0 0 0 1 1 0 0 2 9 7
Nucleolus 3 1 0 1 0 0 0 – 0 0 0 0 2 1 1 1 1 2 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 0 0 1 – 0 2 0 1 1 3 1 1 2 2 2 0 1 0 5 1
Vac/Vac Membrane 109 29 25 83 195 150 273 – 146 198 146 222 115 152 146 63 70 52 141 61 75 139 288 158
Unique Cell Count 148 38 47 161 275 257 476 285 354 283 418 187 286 371 133 121 98 173 71 96 174 353 216
Labelled Cell Count 172 50 57 206 354 332 594 376 443 364 522 229 354 434 164 149 126 173 71 96 174 353 216


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 6.1 5.7 6.1 7.9 5.8 7.7 – 6.3 6.5 6.2 6.3 6.5 6.0 5.7 10.5 12.3 10.8 7.5 7.2 7.9
Std Deviation (1e-4) 2.6 1.5 1.5 1.6 2.1 2.1 2.6 – 2.4 2.4 2.2 2.3 1.8 1.4 1.2 3.6 3.9 3.8 2.1 2.6 2.6
Intensity Change (Log2) – – – 0.09 0.48 0.03 0.44 – 0.14 0.18 0.12 0.14 0.2 0.07 0.01 0.88 1.11 0.93 0.41 0.35 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.3 1.7 1.6 2.7 – 3.3 2.1 4.2 3.6 2.6 4.5 6.6 0.6 -0.4 -0.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.3 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus -1.1 -3.2 -2.6 -2.7 – -2.6 -3.0 -4.6 -5.0 -7.2 0 0 -0.3 -1.4 -0.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -0.2 2.4 0.7 0.5 – -0.2 0.4 -0.2 0 1.0 0 -1.8 -0.7 0.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2925 2.1796 1.2261 0.5716 1.6322 1.8034 1.5603 3.3511 2.7443 1.7353 2.3349 3.4534 3.7107 6.1248 4.8524 4.405 4.994 5.368
Actin 0.0006 0 0 0 0 0 0.02 0 0.0073 0 0 0 0.0092 0 0.0004 0.0394 0.0019 0.0004
Bud 0.0003 0.0004 0.0002 0.0003 0.0001 0.0003 0.0014 0.0009 0.0006 0.0002 0.0003 0.0003 0.0004 0.0002 0.0003 0.002 0.0011 0.0003
Bud Neck 0.0006 0.0008 0.0005 0.0008 0.0006 0.001 0.0015 0.0014 0.0022 0.0008 0.0013 0.0018 0.0009 0.0016 0.001 0.0015 0.0018 0.0017
Bud Periphery 0.0008 0.001 0.0007 0.0007 0.0004 0.0011 0.0024 0.002 0.0012 0.0005 0.0009 0.0011 0.001 0.0007 0.0008 0.0085 0.0027 0.0013
Bud Site 0.0009 0.0011 0.0006 0.0007 0.0003 0.0005 0.0076 0.0025 0.0018 0.0006 0.001 0.0007 0.0013 0.0007 0.0012 0.0031 0.0076 0.0008
Cell Periphery 0.0032 0.0055 0.0031 0.0029 0.0025 0.0045 0.0024 0.0035 0.003 0.0016 0.0017 0.0051 0.0019 0.0035 0.0028 0.0034 0.0035 0.0046
Cytoplasm 0.0054 0.0063 0.0035 0.0011 0.0011 0.0013 0.009 0.0169 0.0072 0.0098 0.0023 0.0067 0.0081 0.0109 0.0026 0.0376 0.0091 0.0015
Cytoplasmic Foci 0.0117 0.0039 0.0081 0.0035 0.0039 0.0023 0.0259 0.0137 0.0195 0.0073 0.0121 0.0076 0.0188 0.0073 0.0059 0.0088 0.0228 0.0059
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0 0 0.0003 0.0001 0
Endoplasmic Reticulum 0.0016 0.0038 0.0012 0.001 0.0009 0.0013 0.0028 0.0042 0.0056 0.0031 0.0012 0.0018 0.0057 0.0025 0.0024 0.0031 0.0036 0.0016
Endosome 0.1781 0.1021 0.1738 0.2027 0.172 0.0932 0.2752 0.176 0.2117 0.2001 0.2858 0.1448 0.2249 0.1086 0.1689 0.1549 0.1579 0.0876
Golgi 0.0082 0.0031 0.0068 0.0068 0.0029 0.0041 0.0146 0.0048 0.0149 0.004 0.0085 0.0037 0.0144 0.0024 0.0062 0.0111 0.0263 0.0033
Lipid Particles 0.0368 0.0076 0.0328 0.0081 0.008 0.0208 0.0325 0.0195 0.0257 0.0207 0.0069 0.02 0.0424 0.008 0.0132 0.0067 0.0535 0.011
Mitochondria 0.0288 0.0187 0.0556 0.0118 0.005 0.0234 0.0216 0.0143 0.0434 0.0057 0.0043 0.0019 0.0214 0.001 0.0238 0.076 0.0317 0.0025
None 0.0052 0.004 0.0033 0.0002 0.0002 0.0001 0.0265 0.0172 0.0099 0.0333 0.0005 0.0206 0.0057 0.0014 0.0004 0.0026 0.0021 0.0002
Nuclear Periphery 0.0031 0.0152 0.0027 0.002 0.0016 0.0052 0.0149 0.0097 0.0116 0.0065 0.0021 0.0029 0.0141 0.0056 0.0073 0.0016 0.0031 0.0034
Nucleolus 0.0064 0.0045 0.0031 0.0034 0.0026 0.0029 0.0142 0.0046 0.0043 0.0031 0.0159 0.0055 0.0044 0.0016 0.0093 0.0029 0.0026 0.0044
Nucleus 0.005 0.007 0.0022 0.0019 0.0016 0.0029 0.0143 0.0085 0.0029 0.0034 0.0308 0.0044 0.0135 0.0034 0.0044 0.0028 0.0031 0.0088
Peroxisomes 0.0032 0.0005 0.0026 0.0007 0.0006 0.0008 0.0061 0.0011 0.0035 0.001 0.0011 0.0007 0.0067 0.0002 0.001 0.0033 0.0103 0.0021
Punctate Nuclear 0.0021 0.0003 0.0002 0.0001 0.0001 0.0001 0.0047 0.0002 0.0004 0.0004 0.0001 0.0002 0.0022 0.0002 0.0006 0.0003 0.0007 0.0009
Vacuole 0.4879 0.6046 0.4827 0.5075 0.6081 0.6157 0.3874 0.561 0.4536 0.5702 0.4648 0.5994 0.4411 0.6702 0.5534 0.5511 0.5499 0.6779
Vacuole Periphery 0.2101 0.2094 0.2163 0.244 0.1874 0.2185 0.1147 0.1379 0.1697 0.1275 0.1583 0.1709 0.1618 0.1698 0.1943 0.079 0.1046 0.1796

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 162.744 145.4832 127.3867 151.0361 167.7488 138.2558 157.027 170.7636 157.2857 156.2602
Translational Efficiency 1.7002 1.5853 1.7484 1.5488 1.5294 1.8929 1.4547 1.5916 1.7228 1.6874

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔