Standard name | |
---|---|
Human Ortholog | |
Description | Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif and is predicted to be an RNA methyltransferase; multicopy suppressor of respiratory defects caused by OXA1 mutations |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 5 | 1 | 3 | 4 | 1 | 10 | 16 |
Bud | 1 | 1 | 7 | 5 | 2 | 3 | 6 | 8 | 5 | 9 | 2 | 11 | 4 | 4 | 1 | 14 | 3 | 7 | 0 | 1 | 0 | 0 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 7 |
Bud Site | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 4 | 5 | 6 | 4 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 6 | 4 | 3 | 2 | 2 | 3 | 1 | 11 | 2 | 2 | 10 | 11 | 7 | 29 | 23 | 12 | 0 | 0 | 0 | 0 | 1 | 3 |
Cytoplasm | 1 | 1 | 8 | 7 | 12 | 11 | 13 | 10 | 8 | 4 | 12 | 5 | 11 | 15 | 30 | 21 | 14 | 14 | 0 | 0 | 1 | 2 | 1 | 0 |
Endoplasmic Reticulum | 0 | 3 | 3 | 1 | 1 | 7 | 2 | 0 | 2 | 1 | 1 | 2 | 6 | 5 | 10 | 18 | 5 | 6 | 0 | 0 | 3 | 0 | 1 | 3 |
Endosome | 4 | 2 | 2 | 1 | 1 | 1 | 4 | 3 | 1 | 1 | 1 | 0 | 1 | 30 | 22 | 24 | 7 | 13 | 8 | 5 | 10 | 7 | 22 | 27 |
Golgi | 0 | 5 | 3 | 4 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 21 | 22 | 43 | 42 | 74 | 54 | 7 | 12 | 9 | 12 | 23 | 22 |
Mitochondria | 151 | 64 | 155 | 128 | 135 | 197 | 250 | 304 | 218 | 269 | 199 | 220 | 168 | 313 | 180 | 31 | 36 | 19 | 123 | 78 | 122 | 135 | 242 | 215 |
Nucleus | 4 | 1 | 1 | 1 | 1 | 3 | 2 | 5 | 3 | 6 | 3 | 1 | 1 | 0 | 0 | 10 | 2 | 7 | 0 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 4 | 0 | 1 | 0 | 3 | 17 | 23 | 23 | 7 | 17 | 14 | 20 | 2 | 1 | 1 | 2 | 4 | 0 | 1 | 0 | 0 | 3 | 3 | 3 |
Nucleolus | 5 | 1 | 5 | 2 | 5 | 23 | 17 | 15 | 19 | 11 | 10 | 7 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 |
Peroxisomes | 3 | 0 | 4 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 21 | 12 | 21 | 5 | 17 | 4 | 4 | 20 | 6 | 18 | 19 |
SpindlePole | 0 | 0 | 2 | 0 | 2 | 1 | 2 | 4 | 3 | 5 | 0 | 1 | 1 | 2 | 0 | 32 | 16 | 13 | 1 | 0 | 0 | 1 | 2 | 1 |
Vac/Vac Membrane | 17 | 12 | 12 | 7 | 13 | 12 | 17 | 17 | 17 | 10 | 8 | 1 | 28 | 64 | 68 | 58 | 53 | 34 | 8 | 9 | 1 | 4 | 15 | 26 |
Unique Cell Count | 157 | 78 | 172 | 137 | 156 | 226 | 281 | 331 | 232 | 280 | 217 | 227 | 209 | 387 | 302 | 249 | 198 | 162 | 160 | 120 | 177 | 180 | 358 | 359 |
Labelled Cell Count | 191 | 94 | 209 | 161 | 183 | 280 | 342 | 397 | 289 | 350 | 256 | 273 | 256 | 489 | 375 | 306 | 246 | 204 | 160 | 120 | 177 | 180 | 358 | 359 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 6.1 | 5.8 | 6.1 | 6.2 | 5.7 | 5.1 | 5.3 | 5.0 | 4.3 | 4.7 | 4.5 | 6.5 | 6.2 | 6.3 | 6.3 | 7.5 | 7.4 | 5.7 | 6.0 | 6.5 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.5 | 1.6 | 1.5 | 1.7 | 1.4 | 1.5 | 1.6 | 1.2 | 1.3 | 1.2 | 1.1 | 1.3 | 1.3 | 1.2 | 1.3 | 1.4 | 1.1 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.1 | -0.02 | -0.19 | -0.14 | -0.22 | -0.45 | -0.31 | -0.37 | 0.16 | 0.1 | 0.12 | 0.11 | 0.36 | 0.35 | -0.03 | 0.05 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.2 | 0 | 0 | 0 | -1.0 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0 | 0.1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | -0.4 | 0 | 3.0 | 2.9 | 1.6 |
Cytoplasm | 0.2 | 1.1 | 0.1 | 0 | -0.9 | -0.6 | 0 | 0.4 | -1.4 | 0.3 | -0.4 | 2.0 | 1.5 | 1.0 | 1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 2.9 | 3.6 | 0 | 3.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 2.1 | 4.4 | 4.9 | 8.4 | 7.7 |
Mitochondria | 1.0 | -1.0 | -0.9 | -0.4 | 0.7 | 1.4 | 0 | 0 | 0 | -2.6 | -2.7 | -7.0 | -15.8 | -13.8 | -14.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 3.3 | 3.5 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2.8 | 1.5 | 0.9 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 1.0 | 2.6 | 0 | 3.1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 3.1 | 3.0 |
Vacuole | -0.7 | 0.5 | -0.7 | -0.4 | -0.8 | 0.1 | -1.6 | -1.5 | -3.6 | 2.0 | 3.0 | 4.3 | 4.4 | 5.0 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7496 | 3.2975 | 2.9463 | 2.2651 | 2.7503 | 2.9724 | 4.5963 | 4.6904 | 4.6132 | 4.6908 | 4.4779 | 4.3525 | 6.5334 | 6.2709 | 6.0141 | 6.3205 | 5.6016 | 5.685 |
Actin | 0.0285 | 0.0053 | 0.0254 | 0.009 | 0.0016 | 0.0038 | 0.0596 | 0.0086 | 0.0159 | 0.0343 | 0.0007 | 0.0101 | 0.0239 | 0.0141 | 0.0247 | 0.0397 | 0.0064 | 0.0085 |
Bud | 0.0008 | 0.0007 | 0.007 | 0.0012 | 0.0003 | 0.0002 | 0.001 | 0.0011 | 0.0007 | 0.0004 | 0.0004 | 0.0004 | 0.0009 | 0.0012 | 0.003 | 0.0004 | 0.0003 | 0.0005 |
Bud Neck | 0.0011 | 0.0003 | 0.0006 | 0.0059 | 0.0001 | 0.0033 | 0.0011 | 0.0007 | 0.0006 | 0.0031 | 0 | 0.0013 | 0.004 | 0.001 | 0.0013 | 0.0006 | 0.0002 | 0.0004 |
Bud Periphery | 0.0021 | 0.0015 | 0.007 | 0.0034 | 0.0014 | 0.0006 | 0.0024 | 0.002 | 0.0017 | 0.0022 | 0.0022 | 0.0008 | 0.0017 | 0.0025 | 0.004 | 0.0038 | 0.0008 | 0.0007 |
Bud Site | 0.0064 | 0.0026 | 0.0098 | 0.001 | 0.0003 | 0.0006 | 0.0054 | 0.0067 | 0.0021 | 0.0008 | 0.0001 | 0.0009 | 0.0044 | 0.0344 | 0.0158 | 0.0018 | 0.0004 | 0.0006 |
Cell Periphery | 0.0009 | 0.0005 | 0.0022 | 0.0004 | 0.0003 | 0.0003 | 0.0008 | 0.0007 | 0.013 | 0.0012 | 0.0004 | 0.0002 | 0.0009 | 0.0023 | 0.0019 | 0.0049 | 0.0001 | 0.0003 |
Cytoplasm | 0.0024 | 0.0012 | 0.0013 | 0.0004 | 0.0004 | 0.0007 | 0.0051 | 0.0031 | 0.0018 | 0.0014 | 0.0015 | 0.0016 | 0.0082 | 0.0038 | 0.0042 | 0.0231 | 0.0002 | 0.0039 |
Cytoplasmic Foci | 0.0172 | 0.0179 | 0.0063 | 0.0075 | 0.0132 | 0.0132 | 0.0245 | 0.027 | 0.0237 | 0.0052 | 0.0083 | 0.0122 | 0.031 | 0.0219 | 0.0243 | 0.0037 | 0.0099 | 0.0194 |
Eisosomes | 0.0007 | 0.0002 | 0.0017 | 0.0005 | 0.0002 | 0.0006 | 0.0007 | 0.0002 | 0.0019 | 0.0073 | 0.0003 | 0.0002 | 0.0005 | 0.001 | 0.001 | 0.001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0037 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0032 | 0.0007 | 0.001 | 0.0002 | 0.0014 | 0.0005 | 0.0089 | 0.0014 | 0.0016 | 0.0136 | 0.0002 | 0.0004 |
Endosome | 0.0706 | 0.0563 | 0.0134 | 0.0058 | 0.0687 | 0.0249 | 0.0995 | 0.0842 | 0.035 | 0.0258 | 0.0537 | 0.0223 | 0.0794 | 0.0393 | 0.0595 | 0.0098 | 0.0288 | 0.0358 |
Golgi | 0.047 | 0.0423 | 0.027 | 0.0114 | 0.0544 | 0.0212 | 0.0738 | 0.0628 | 0.0423 | 0.0364 | 0.0547 | 0.0419 | 0.0595 | 0.0487 | 0.0541 | 0.0068 | 0.053 | 0.0495 |
Lipid Particles | 0.0398 | 0.0258 | 0.0059 | 0.0055 | 0.0172 | 0.0231 | 0.0416 | 0.022 | 0.0063 | 0.0014 | 0.0019 | 0.0056 | 0.0379 | 0.0112 | 0.0165 | 0.0015 | 0.0047 | 0.0147 |
Mitochondria | 0.7167 | 0.7874 | 0.8603 | 0.9126 | 0.8222 | 0.861 | 0.6069 | 0.7392 | 0.8186 | 0.8704 | 0.8477 | 0.8644 | 0.6372 | 0.7704 | 0.7446 | 0.8534 | 0.8589 | 0.8249 |
None | 0.0089 | 0.0002 | 0.0038 | 0.0005 | 0.0003 | 0.0015 | 0.0048 | 0.0008 | 0.0011 | 0.0012 | 0.002 | 0.0048 | 0.007 | 0.0118 | 0.0034 | 0.0105 | 0.0001 | 0.0013 |
Nuclear Periphery | 0.0082 | 0.0003 | 0.0003 | 0 | 0.0002 | 0.0004 | 0.0147 | 0.0002 | 0.0003 | 0 | 0.0008 | 0.0021 | 0.012 | 0.0004 | 0.0011 | 0.0044 | 0 | 0.0002 |
Nucleolus | 0.0012 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0011 | 0.0027 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0016 | 0.0009 | 0.0002 | 0.0001 | 0.0002 |
Nucleus | 0.0015 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0027 | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0.0031 | 0.0099 | 0.0008 | 0.0006 | 0.0015 | 0.0001 | 0.0002 |
Peroxisomes | 0.0247 | 0.0262 | 0.0234 | 0.0325 | 0.0134 | 0.033 | 0.0254 | 0.0202 | 0.0209 | 0.005 | 0.0051 | 0.0166 | 0.0534 | 0.0167 | 0.0186 | 0.0144 | 0.0332 | 0.0294 |
Punctate Nuclear | 0.0016 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0036 | 0.0052 | 0.0002 | 0.0003 | 0 | 0 | 0.0003 | 0.0034 | 0.0003 | 0.0044 | 0.0014 | 0 | 0.0002 |
Vacuole | 0.0088 | 0.0181 | 0.0014 | 0.0008 | 0.0021 | 0.0034 | 0.0122 | 0.0135 | 0.0092 | 0.0014 | 0.0078 | 0.0067 | 0.0094 | 0.0107 | 0.009 | 0.0018 | 0.0008 | 0.0038 |
Vacuole Periphery | 0.0071 | 0.0114 | 0.0022 | 0.0011 | 0.003 | 0.003 | 0.0066 | 0.0047 | 0.0031 | 0.0021 | 0.0105 | 0.0039 | 0.0058 | 0.0045 | 0.0053 | 0.0016 | 0.0017 | 0.0051 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.5197 | 13.2353 | 8.2704 | 10.0461 | 13.1342 | 10.9033 | 9.5084 | 12.835 | 12.2829 | 11.2271 |
Translational Efficiency | 0.9554 | 0.6104 | 1.002 | 1.1476 | 0.5079 | 0.8402 | 1.1745 | 0.8518 | 0.8916 | 0.9021 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2508 | 1441 | 2231 | 1623 | 751 | 1855 | 182 | 251 | 3259 | 3296 | 2413 | 1874 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 734.93 | 821.73 | 908.42 | 895.70 | 702.27 | 797.64 | 1046.52 | 986.65 | 727.40 | 808.17 | 918.84 | 907.88 |
Standard Deviation | 85.21 | 119.11 | 112.37 | 109.82 | 94.00 | 117.76 | 93.55 | 127.25 | 88.39 | 118.96 | 116.90 | 116.50 |
Intensity Change Log 2 | — | 0.161058 | 0.305753 | 0.285409 | — | 0.183712 | 0.575502 | 0.490513 | — | 0.172172 | 0.443863 | 0.389274 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000242 | 0.001020 | 0.000611 | 0.027921 | 0.000398 | 0.000479 | 0.021288 | 0.017559 | 0.000278 | 0.000715 | 0.002170 | 0.026533 |
Bud Neck | 0.014664 | 0.001768 | 0.005694 | 0.008017 | 0.007170 | 0.003436 | 0.018813 | 0.011711 | 0.012937 | 0.002707 | 0.006683 | 0.008512 |
Bud Site | 0.019841 | 0.012654 | 0.018810 | 0.149621* | 0.008983 | 0.010176 | 0.111014* | 0.164869* | 0.017339 | 0.011259 | 0.025764 | 0.151663* |
Cell Periphery | 0.000368 | 0.000399 | 0.000120 | 0.000845 | 0.000371 | 0.000346 | 0.000769 | 0.000461 | 0.000369 | 0.000370 | 0.000169 | 0.000794 |
Cytoplasm | 0.012552 | 0.002477 | 0.007257 | 0.042766 | 0.007726 | 0.002103 | 0.035710 | 0.054310 | 0.011440 | 0.002267 | 0.009403 | 0.044312 |
Cytoplasmic Foci | 0.063678 | 0.030128 | 0.044793 | 0.090942 | 0.048658 | 0.024017 | 0.190133* | 0.156304 | 0.060217 | 0.026688 | 0.055755 | 0.099696 |
Eisosomes | 0.000082 | 0.000056 | 0.000042 | 0.000179 | 0.000075 | 0.000047 | 0.000188 | 0.000134 | 0.000080 | 0.000050 | 0.000053 | 0.000173 |
Endoplasmic Reticulum | 0.001512 | 0.000384 | 0.000994 | 0.002107 | 0.000738 | 0.000365 | 0.007124 | 0.002127 | 0.001334 | 0.000374 | 0.001457 | 0.002109 |
Endosome | 0.099004 | 0.071443 | 0.083282 | 0.072014 | 0.065314 | 0.063888 | 0.151503 | 0.094336 | 0.091240 | 0.067191 | 0.088427 | 0.075004 |
Golgi | 0.099590 | 0.091688 | 0.088576 | 0.120788* | 0.087479 | 0.078884 | 0.081286 | 0.105257 | 0.096799 | 0.084482 | 0.088026 | 0.118708* |
Lipid Particles | 0.007025 | 0.001187 | 0.000770 | 0.000711 | 0.006328 | 0.001195 | 0.009440 | 0.003988 | 0.006865 | 0.001192 | 0.001424 | 0.001150 |
Mitochondria | 0.557814* | 0.712228* | 0.475673* | 0.295072* | 0.669768* | 0.737593* | 0.025489 | 0.117120* | 0.583612* | 0.726504* | 0.441718* | 0.271237* |
Mitotic Spindle | 0.010437 | 0.011955 | 0.062021 | 0.044707 | 0.014735 | 0.007509 | 0.085279 | 0.080319 | 0.011428 | 0.009453 | 0.063775 | 0.049476 |
None | 0.002382 | 0.000296 | 0.000416 | 0.000318 | 0.000787 | 0.000680 | 0.000697 | 0.000214 | 0.002015 | 0.000512 | 0.000437 | 0.000304 |
Nuclear Periphery | 0.000411 | 0.000173 | 0.000855 | 0.000118 | 0.000643 | 0.000087 | 0.001107 | 0.000089 | 0.000465 | 0.000125 | 0.000874 | 0.000114 |
Nuclear Periphery Foci | 0.000463 | 0.000303 | 0.000683 | 0.000233 | 0.000387 | 0.000152 | 0.002979 | 0.000820 | 0.000445 | 0.000218 | 0.000856 | 0.000312 |
Nucleolus | 0.002101 | 0.000607 | 0.001446 | 0.000155 | 0.002812 | 0.001033 | 0.004881 | 0.000192 | 0.002265 | 0.000846 | 0.001705 | 0.000160 |
Nucleus | 0.002522 | 0.000909 | 0.003299 | 0.000975 | 0.004199 | 0.000953 | 0.017732 | 0.002841 | 0.002909 | 0.000934 | 0.004388 | 0.001225 |
Peroxisomes | 0.014265 | 0.020593 | 0.008912 | 0.058681 | 0.033052 | 0.012967 | 0.111202* | 0.088774 | 0.018594 | 0.016301 | 0.016627 | 0.062711 |
Vacuole | 0.046634 | 0.020313 | 0.123562 | 0.062852 | 0.014379 | 0.025094 | 0.099086 | 0.082694 | 0.039201 | 0.023004 | 0.121716 | 0.065510 |
Vacuole Periphery | 0.044413 | 0.019420 | 0.072185 | 0.020978 | 0.025998 | 0.028997 | 0.024278 | 0.015880 | 0.040169 | 0.024810 | 0.068571 | 0.020295 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.76 | -4.54 | -11.49 | -11.10 | -11.33 | -0.75 | -4.01 | -4.22 | -4.20 | 0.55 | -3.19 | -4.62 | -12.17 | -11.95 | -11.09 |
Bud Neck | 11.59 | 7.41 | 5.29 | -10.67 | -3.17 | 2.36 | -2.90 | -2.19 | -4.51 | 1.51 | 10.53 | 5.82 | 3.94 | -9.31 | -2.52 |
Bud Site | 3.56 | 0.33 | -24.57 | -25.80* | -24.87* | -0.64 | -8.09 | -12.09 | -12.01 | -3.01 | 4.12 | -4.62 | -27.59 | -29.01 | -25.19 |
Cell Periphery | -0.57 | 8.29 | -3.07 | -2.78 | -4.78 | 0.27 | -1.46 | -0.72 | -0.99 | 0.98 | -0.03 | 5.66 | -3.13 | -3.11 | -4.69 |
Cytoplasm | 8.86 | 3.97 | -12.64 | -18.08 | -15.47 | 2.89 | -4.56 | -7.37 | -8.59 | -2.29 | 9.46 | 1.41 | -14.73 | -20.16 | -15.84 |
Cytoplasmic Foci | 12.68 | 7.32 | -7.53 | -18.06 | -13.89 | 7.12 | -12.09 | -10.39 | -13.46 | 2.33 | 16.46 | 1.88 | -11.52 | -23.75 | -12.78 |
Eisosomes | 6.98 | 11.83 | -13.13 | -17.42 | -19.93 | 5.73 | -6.15 | -4.69 | -7.45 | 2.68 | 10.07 | 8.13 | -14.08 | -19.72 | -18.72 |
Endoplasmic Reticulum | 5.96 | 2.48 | -1.87 | -5.75 | -3.63 | 2.79 | -4.75 | -3.23 | -4.17 | 3.47 | 6.66 | -0.86 | -2.79 | -6.66 | -2.28 |
Endosome | 6.59 | 3.99 | 6.81 | -0.02 | 3.11 | 0.26 | -6.75 | -3.18 | -3.65 | 4.15 | 7.48 | 0.86 | 4.73 | -2.21 | 3.83 |
Golgi | 1.57 | 2.64 | -3.69 | -4.71 | -5.90 | 1.35 | 0.90 | -1.37 | -2.44 | -1.91 | 3.33 | 2.38 | -4.22 | -6.98 | -6.07 |
Lipid Particles | 7.17 | 8.72 | 8.87 | 0.99 | 0.31 | 4.31 | -1.11 | 1.04 | -1.53 | 1.69 | 8.81 | 8.28 | 8.62 | 0.06 | 0.76 |
Mitochondria | -14.46 | 8.67 | 26.04 | 36.66* | 17.26* | -4.87 | 48.44* | 31.43* | 42.78* | -6.40 | -17.89 | 15.69 | 34.24 | 51.24* | 17.25* |
Mitotic Spindle | -0.82 | -16.08 | -11.20 | -9.99 | 4.11 | 3.15 | -5.28 | -6.03 | -6.78 | 0.14 | 1.61 | -16.74 | -12.53 | -13.22 | 3.38 |
None | 3.62 | 3.41 | 3.57 | -0.27 | 1.48 | 0.60 | 0.17 | 2.62 | 1.53 | 1.20 | 3.27 | 3.55 | 3.82 | 1.42 | 1.92 |
Nuclear Periphery | 2.11 | -2.99 | 4.19 | 0.46 | 5.24 | 1.16 | -0.71 | 1.10 | -1.14 | 2.11 | 2.67 | -2.35 | 2.76 | -0.06 | 5.62 |
Nuclear Periphery Foci | 1.23 | -1.77 | 0.73 | -0.27 | 1.86 | 2.20 | -4.50 | -0.91 | -1.36 | 2.99 | 3.08 | -3.33 | 0.18 | -1.28 | 2.53 |
Nucleolus | 3.60 | 1.55 | 5.87 | 1.71 | 5.00 | 1.68 | -0.86 | 2.60 | 1.74 | 1.86 | 3.26 | 1.13 | 6.13 | 2.52 | 4.96 |
Nucleus | 2.18 | -1.58 | 3.78 | -0.29 | 3.86 | 2.68 | -2.65 | -0.47 | -1.69 | 2.11 | 4.08 | -2.22 | 1.90 | -1.63 | 3.52 |
Peroxisomes | -2.84 | 4.15 | -12.93 | -9.90 | -14.62 | 5.43 | -5.84 | -5.10 | -7.37 | 1.39 | 1.49 | 1.21 | -13.09 | -13.77 | -13.36 |
Vacuole | 10.62 | -19.61 | -5.09 | -12.37 | 12.13 | -5.00 | -8.58 | -7.84 | -6.70 | 1.17 | 8.65 | -22.93 | -8.57 | -13.81 | 11.81 |
Vacuole Periphery | 11.64 | -9.06 | 10.76 | -0.69 | 17.83* | -1.00 | 0.52 | 3.07 | 5.08 | 1.87 | 8.58 | -10.16 | 10.53 | 2.97 | 18.14* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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