Standard name
Human Ortholog
Description Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif and is predicted to be an RNA methyltransferase; multicopy suppressor of respiratory defects caused by OXA1 mutations

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.12 0.12 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.05 0.08 0.05 0.05 0 0 0 0.06 0 0.05 0 0.1 0.08 0.07 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.1 0 0.08 0.05 0 0.06 0 0.06 0.08
Golgi 0 0.06 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0.14 0.17 0.37 0.33 0.05 0.1 0.05 0.07 0.06 0.06
Mitochondria 0.96 0.82 0.9 0.93 0.87 0.87 0.89 0.92 0.94 0.96 0.92 0.97 0.8 0.81 0.6 0.12 0.18 0.12 0.77 0.66 0.69 0.75 0.68 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.08 0.08 0.07 0 0.06 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.1 0.06 0.05 0.08 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0.1 0 0 0.12 0 0.05 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.08 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.15 0.07 0.05 0.08 0.05 0.06 0.05 0.07 0 0 0 0.13 0.17 0.23 0.23 0.27 0.21 0.05 0.08 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 0 0 0 0 0 1 0 1 1 3 5 1 3 4 1 10 16
Bud 1 1 7 5 2 3 6 8 5 9 2 11 4 4 1 14 3 7 0 1 0 0 3 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 7
Bud Site 1 0 0 0 1 2 1 4 5 6 4 2 0 0 0 2 0 2
Cell Periphery 0 3 6 4 3 2 2 3 1 11 2 2 10 11 7 29 23 12 0 0 0 0 1 3
Cytoplasm 1 1 8 7 12 11 13 10 8 4 12 5 11 15 30 21 14 14 0 0 1 2 1 0
Endoplasmic Reticulum 0 3 3 1 1 7 2 0 2 1 1 2 6 5 10 18 5 6 0 0 3 0 1 3
Endosome 4 2 2 1 1 1 4 3 1 1 1 0 1 30 22 24 7 13 8 5 10 7 22 27
Golgi 0 5 3 4 3 1 1 0 0 0 0 0 21 22 43 42 74 54 7 12 9 12 23 22
Mitochondria 151 64 155 128 135 197 250 304 218 269 199 220 168 313 180 31 36 19 123 78 122 135 242 215
Nucleus 4 1 1 1 1 3 2 5 3 6 3 1 1 0 0 10 2 7 0 0 0 0 2 0
Nuclear Periphery 4 0 1 0 3 17 23 23 7 17 14 20 2 1 1 2 4 0 1 0 0 3 3 3
Nucleolus 5 1 5 2 5 23 17 15 19 11 10 7 1 1 0 1 0 1 0 0 0 0 2 1
Peroxisomes 3 0 4 1 1 0 1 1 0 0 0 1 1 21 12 21 5 17 4 4 20 6 18 19
SpindlePole 0 0 2 0 2 1 2 4 3 5 0 1 1 2 0 32 16 13 1 0 0 1 2 1
Vac/Vac Membrane 17 12 12 7 13 12 17 17 17 10 8 1 28 64 68 58 53 34 8 9 1 4 15 26
Unique Cell Count 157 78 172 137 156 226 281 331 232 280 217 227 209 387 302 249 198 162 160 120 177 180 358 359
Labelled Cell Count 191 94 209 161 183 280 342 397 289 350 256 273 256 489 375 306 246 204 160 120 177 180 358 359


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.1 5.8 6.1 6.2 5.7 5.1 5.3 5.0 4.3 4.7 4.5 6.5 6.2 6.3 6.3 7.5 7.4 5.7 6.0 6.5
Std Deviation (1e-4) 0.7 1.1 1.5 1.6 1.5 1.7 1.4 1.5 1.6 1.2 1.3 1.2 1.1 1.3 1.3 1.2 1.3 1.4 1.1 1.6 1.4
Intensity Change (Log2) 0.07 0.1 -0.02 -0.19 -0.14 -0.22 -0.45 -0.31 -0.37 0.16 0.1 0.12 0.11 0.36 0.35 -0.03 0.05 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.2 0 0 0 -1.0 -1.1 0 0 0 0 0 0 0.7 0 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.6 -0.4 0 3.0 2.9 1.6
Cytoplasm 0.2 1.1 0.1 0 -0.9 -0.6 0 0.4 -1.4 0.3 -0.4 2.0 1.5 1.0 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 3.1 2.9 3.6 0 3.0
Golgi 0 0 0 0 0 0 0 0 0 3.3 2.1 4.4 4.9 8.4 7.7
Mitochondria 1.0 -1.0 -0.9 -0.4 0.7 1.4 0 0 0 -2.6 -2.7 -7.0 -15.8 -13.8 -14.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 3.3 3.5 3.2 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.8 1.5 0.9 2.2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1.6 1.0 2.6 0 3.1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 4.3 3.1 3.0
Vacuole -0.7 0.5 -0.7 -0.4 -0.8 0.1 -1.6 -1.5 -3.6 2.0 3.0 4.3 4.4 5.0 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7496 3.2975 2.9463 2.2651 2.7503 2.9724 4.5963 4.6904 4.6132 4.6908 4.4779 4.3525 6.5334 6.2709 6.0141 6.3205 5.6016 5.685
Actin 0.0285 0.0053 0.0254 0.009 0.0016 0.0038 0.0596 0.0086 0.0159 0.0343 0.0007 0.0101 0.0239 0.0141 0.0247 0.0397 0.0064 0.0085
Bud 0.0008 0.0007 0.007 0.0012 0.0003 0.0002 0.001 0.0011 0.0007 0.0004 0.0004 0.0004 0.0009 0.0012 0.003 0.0004 0.0003 0.0005
Bud Neck 0.0011 0.0003 0.0006 0.0059 0.0001 0.0033 0.0011 0.0007 0.0006 0.0031 0 0.0013 0.004 0.001 0.0013 0.0006 0.0002 0.0004
Bud Periphery 0.0021 0.0015 0.007 0.0034 0.0014 0.0006 0.0024 0.002 0.0017 0.0022 0.0022 0.0008 0.0017 0.0025 0.004 0.0038 0.0008 0.0007
Bud Site 0.0064 0.0026 0.0098 0.001 0.0003 0.0006 0.0054 0.0067 0.0021 0.0008 0.0001 0.0009 0.0044 0.0344 0.0158 0.0018 0.0004 0.0006
Cell Periphery 0.0009 0.0005 0.0022 0.0004 0.0003 0.0003 0.0008 0.0007 0.013 0.0012 0.0004 0.0002 0.0009 0.0023 0.0019 0.0049 0.0001 0.0003
Cytoplasm 0.0024 0.0012 0.0013 0.0004 0.0004 0.0007 0.0051 0.0031 0.0018 0.0014 0.0015 0.0016 0.0082 0.0038 0.0042 0.0231 0.0002 0.0039
Cytoplasmic Foci 0.0172 0.0179 0.0063 0.0075 0.0132 0.0132 0.0245 0.027 0.0237 0.0052 0.0083 0.0122 0.031 0.0219 0.0243 0.0037 0.0099 0.0194
Eisosomes 0.0007 0.0002 0.0017 0.0005 0.0002 0.0006 0.0007 0.0002 0.0019 0.0073 0.0003 0.0002 0.0005 0.001 0.001 0.001 0.0001 0.0002
Endoplasmic Reticulum 0.0037 0.0004 0.0004 0.0001 0.0003 0.0002 0.0032 0.0007 0.001 0.0002 0.0014 0.0005 0.0089 0.0014 0.0016 0.0136 0.0002 0.0004
Endosome 0.0706 0.0563 0.0134 0.0058 0.0687 0.0249 0.0995 0.0842 0.035 0.0258 0.0537 0.0223 0.0794 0.0393 0.0595 0.0098 0.0288 0.0358
Golgi 0.047 0.0423 0.027 0.0114 0.0544 0.0212 0.0738 0.0628 0.0423 0.0364 0.0547 0.0419 0.0595 0.0487 0.0541 0.0068 0.053 0.0495
Lipid Particles 0.0398 0.0258 0.0059 0.0055 0.0172 0.0231 0.0416 0.022 0.0063 0.0014 0.0019 0.0056 0.0379 0.0112 0.0165 0.0015 0.0047 0.0147
Mitochondria 0.7167 0.7874 0.8603 0.9126 0.8222 0.861 0.6069 0.7392 0.8186 0.8704 0.8477 0.8644 0.6372 0.7704 0.7446 0.8534 0.8589 0.8249
None 0.0089 0.0002 0.0038 0.0005 0.0003 0.0015 0.0048 0.0008 0.0011 0.0012 0.002 0.0048 0.007 0.0118 0.0034 0.0105 0.0001 0.0013
Nuclear Periphery 0.0082 0.0003 0.0003 0 0.0002 0.0004 0.0147 0.0002 0.0003 0 0.0008 0.0021 0.012 0.0004 0.0011 0.0044 0 0.0002
Nucleolus 0.0012 0.0006 0.0002 0.0002 0.0001 0.0011 0.0027 0.0008 0.0002 0.0001 0.0001 0.0002 0.0007 0.0016 0.0009 0.0002 0.0001 0.0002
Nucleus 0.0015 0.0004 0.0003 0.0001 0.0001 0.0003 0.0027 0.0005 0.0003 0.0001 0.0001 0.0031 0.0099 0.0008 0.0006 0.0015 0.0001 0.0002
Peroxisomes 0.0247 0.0262 0.0234 0.0325 0.0134 0.033 0.0254 0.0202 0.0209 0.005 0.0051 0.0166 0.0534 0.0167 0.0186 0.0144 0.0332 0.0294
Punctate Nuclear 0.0016 0.0002 0.0001 0.0001 0.0002 0.0036 0.0052 0.0002 0.0003 0 0 0.0003 0.0034 0.0003 0.0044 0.0014 0 0.0002
Vacuole 0.0088 0.0181 0.0014 0.0008 0.0021 0.0034 0.0122 0.0135 0.0092 0.0014 0.0078 0.0067 0.0094 0.0107 0.009 0.0018 0.0008 0.0038
Vacuole Periphery 0.0071 0.0114 0.0022 0.0011 0.003 0.003 0.0066 0.0047 0.0031 0.0021 0.0105 0.0039 0.0058 0.0045 0.0053 0.0016 0.0017 0.0051

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.5197 13.2353 8.2704 10.0461 13.1342 10.9033 9.5084 12.835 12.2829 11.2271
Translational Efficiency 0.9554 0.6104 1.002 1.1476 0.5079 0.8402 1.1745 0.8518 0.8916 0.9021

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif and is predicted to be an RNA methyltransferase; multicopy suppressor of respiratory defects caused by OXA1 mutations
Localization
Cell Percentages mitochondrion (92%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Oms1

Oms1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Oms1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available