Standard name
Description Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif and is predicted to be an RNA methyltransferase; multicopy suppressor of respiratory defects caused by OXA1 mutations

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.12 0.12 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.05 0.08 0.05 0.05 0 0 0 0.06 0 0.05 0 0.1 0.08 0.07 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.1 0 0.08 0.05 0 0.06 0 0.06 0.08
Golgi 0 0.06 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0.14 0.17 0.37 0.33 0.05 0.1 0.05 0.07 0.06 0.06
Mitochondria 0.96 0.82 0.9 0.93 0.87 0.87 0.89 0.92 0.94 0.96 0.92 0.97 0.8 0.81 0.6 0.12 0.18 0.12 0.77 0.66 0.69 0.75 0.68 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.08 0.08 0.07 0 0.06 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.1 0.06 0.05 0.08 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0.1 0 0 0.12 0 0.05 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.08 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.15 0.07 0.05 0.08 0.05 0.06 0.05 0.07 0 0 0 0.13 0.17 0.23 0.23 0.27 0.21 0.05 0.08 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 0 0 0 0 0 1 0 1 1 3 5 1 3 4 1 10 16
Bud 1 1 7 5 2 3 6 8 5 9 2 11 4 4 1 14 3 7 0 1 0 0 3 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 7
Bud Site 1 0 0 0 1 2 1 4 5 6 4 2 0 0 0 2 0 2
Cell Periphery 0 3 6 4 3 2 2 3 1 11 2 2 10 11 7 29 23 12 0 0 0 0 1 3
Cytoplasm 1 1 8 7 12 11 13 10 8 4 12 5 11 15 30 21 14 14 0 0 1 2 1 0
Endoplasmic Reticulum 0 3 3 1 1 7 2 0 2 1 1 2 6 5 10 18 5 6 0 0 3 0 1 3
Endosome 4 2 2 1 1 1 4 3 1 1 1 0 1 30 22 24 7 13 8 5 10 7 22 27
Golgi 0 5 3 4 3 1 1 0 0 0 0 0 21 22 43 42 74 54 7 12 9 12 23 22
Mitochondria 151 64 155 128 135 197 250 304 218 269 199 220 168 313 180 31 36 19 123 78 122 135 242 215
Nucleus 4 1 1 1 1 3 2 5 3 6 3 1 1 0 0 10 2 7 0 0 0 0 2 0
Nuclear Periphery 4 0 1 0 3 17 23 23 7 17 14 20 2 1 1 2 4 0 1 0 0 3 3 3
Nucleolus 5 1 5 2 5 23 17 15 19 11 10 7 1 1 0 1 0 1 0 0 0 0 2 1
Peroxisomes 3 0 4 1 1 0 1 1 0 0 0 1 1 21 12 21 5 17 4 4 20 6 18 19
SpindlePole 0 0 2 0 2 1 2 4 3 5 0 1 1 2 0 32 16 13 1 0 0 1 2 1
Vac/Vac Membrane 17 12 12 7 13 12 17 17 17 10 8 1 28 64 68 58 53 34 8 9 1 4 15 26
Unique Cell Count 157 78 172 137 156 226 281 331 232 280 217 227 209 387 302 249 198 162 160 120 177 180 358 359
Labelled Cell Count 191 94 209 161 183 280 342 397 289 350 256 273 256 489 375 306 246 204 160 120 177 180 358 359


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.1 5.8 6.1 6.2 5.7 5.1 5.3 5.0 4.3 4.7 4.5 6.5 6.2 6.3 6.3 7.5 7.4 5.7 6.0 6.5
Std Deviation (1e-4) 0.7 1.1 1.5 1.6 1.5 1.7 1.4 1.5 1.6 1.2 1.3 1.2 1.1 1.3 1.3 1.2 1.3 1.4 1.1 1.6 1.4
Intensity Change (Log2) 0.07 0.1 -0.02 -0.19 -0.14 -0.22 -0.45 -0.31 -0.37 0.16 0.1 0.12 0.11 0.36 0.35 -0.03 0.05 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.2 0 0 0 -1.0 -1.1 0 0 0 0 0 0 0.7 0 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.6 -0.4 0 3.0 2.9 1.6
Cytoplasm 0.2 1.1 0.1 0 -0.9 -0.6 0 0.4 -1.4 0.3 -0.4 2.0 1.5 1.0 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 3.1 2.9 3.6 0 3.0
Golgi 0 0 0 0 0 0 0 0 0 3.3 2.1 4.4 4.9 8.4 7.7
Mitochondria 1.0 -1.0 -0.9 -0.4 0.7 1.4 0 0 0 -2.6 -2.7 -7.0 -15.8 -13.8 -14.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 3.3 3.5 3.2 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.8 1.5 0.9 2.2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1.6 1.0 2.6 0 3.1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 4.3 3.1 3.0
Vacuole -0.7 0.5 -0.7 -0.4 -0.8 0.1 -1.6 -1.5 -3.6 2.0 3.0 4.3 4.4 5.0 3.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Oms1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available