Standard name
Human Ortholog
Description Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding by the complex; required for tRNA genes clustering at the nucleolus; required for replication slow zone breakage following Mec1p inactivation; transcription is cell cycle regulated, peaking in mitosis and declining in G1; protein is constitutively degraded by the proteasome; rate limiting for condensin recruitment to chromatin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0.05 0.07 0 0 0 – 0.05 0 0.12 0.14 0.16 0.07 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0.06 0 0 0 0 – 0 0 0 0.05 0 0.07 0.11 0.11 0.09 0 0.09 0.14 0.18 0.2
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.11 0.74 0.73 0.4 0.56 0.62 0.58 0.7 – 0.74 0.83 0.05 0.05 0.24 0.06 0 0.11 0 0 0 0 0 0
Nucleus 0.63 0.73 0.42 0.48 0.37 0.39 0.44 0.45 0.47 – 0.45 0.37 0.75 0.59 0.47 0.6 0.49 0.39 0.32 0.6 0.38 0.28 0.23 0.19
Nuclear Periphery 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0.06 0 0.06 0 0 0
Nucleolus 0.32 0.18 0.16 0.25 0.32 0.31 0.21 0.21 0.24 – 0.14 0.2 0.14 0.23 0.25 0.25 0.34 0.4 0.29 0.2 0.2 0.27 0.17 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.08 0 0 0 0.19 0.11 0.08 0.1 0.09 – 0.05 0 0 0 0 0 0.06 0.08 0.13 0.06 0.07 0.16 0.18 0.2
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.06 0.07 0 0.06 0.11 0.07 0.13 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud 0 0 0 2 2 1 0 4 5 – 2 3 0 0 1 0 0 3 3 2 0 2 6 3
Bud Neck 0 0 0 0 0 1 2 0 0 – 1 0 0 0 0 0 0 0 1 0 0 1 0 2
Bud Site 0 0 0 1 1 3 4 8 6 – 8 15 0 0 0 1 0 1 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 0 6 7 11 7 12 8 – 6 9 32 53 78 15 9 18 0 0 0 2 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 3 3 2 0 0 0 1 1 0
Endosome 12 3 0 0 9 2 4 0 0 – 0 1 2 20 13 16 18 22 22 9 5 33 59 89
Golgi 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 0 0 1 2 2
Mitochondria 15 24 14 52 62 83 136 173 148 – 98 182 13 17 115 12 6 22 3 2 2 2 4 13
Nucleus 145 159 8 34 57 57 97 133 100 – 60 82 207 222 221 128 79 79 83 145 24 69 77 83
Nuclear Periphery 7 10 0 2 2 1 3 6 5 – 1 7 6 9 7 9 10 6 14 2 3 2 2 7
Nucleolus 73 38 3 18 50 45 47 61 51 – 19 43 38 85 121 54 54 82 74 48 12 66 55 76
Peroxisomes 0 0 0 0 0 1 1 1 0 – 0 0 0 1 0 0 1 1 1 0 0 0 0 2
SpindlePole 19 3 0 0 30 16 17 29 19 – 7 8 0 7 14 6 10 16 34 13 4 39 58 88
Vac/Vac Membrane 6 11 0 0 0 2 5 2 0 – 2 5 4 9 13 17 9 14 9 14 7 16 43 58
Unique Cell Count 229 217 19 71 155 147 219 297 211 132 220 277 376 475 214 161 204 259 243 64 248 328 450
Labelled Cell Count 280 253 25 115 220 223 323 429 342 204 355 302 423 584 261 199 266 259 243 64 248 328 450


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.2 3.7 4.0 4.2 3.8 3.6 3.7 3.2 – 3.3 2.8 5.6 5.7 4.8 8.1 9.0 9.4 5.7 5.1 5.9
Std Deviation (1e-4) 0.9 0.8 0.3 1.2 1.4 1.3 1.1 1.2 1.2 – 1.2 0.7 1.3 1.4 1.3 2.3 2.4 2.9 1.3 1.3 1.3
Intensity Change (Log2) – – – 0.09 0.15 0.01 -0.07 -0.02 -0.23 – -0.18 -0.4 0.59 0.62 0.36 1.11 1.27 1.33 0.6 0.45 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0.4 -0.5 -0.3 0.2 0.2 0.4 – 0.3 -0.4 3.1 1.5 0.4 1.5 0.6 -0.3
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 1.6 2.1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.019 1.6757 1.8376 1.6545 1.5313 1.4015 3.2021 2.5995 2.6106 2.1116 2.0271 2.3309 0.7623 1.0011 0.8362 0.655 0.474 0.7083
Actin 0.0464 0.0005 0.0119 0.0021 0.0465 0.0005 0.0403 0.0002 0.0227 0.0026 0.0007 0.0046 0.0222 0.0004 0.0063 0.0008 0.0004 0.0003
Bud 0.0008 0.0003 0.0003 0.0003 0.0003 0.0001 0.001 0.0001 0.001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Neck 0.0037 0.0026 0.0054 0.0132 0.0604 0.0052 0.0031 0.0014 0.0027 0.0014 0.0068 0.0035 0.0014 0.0008 0.007 0.0019 0.047 0.0047
Bud Periphery 0.0019 0.0011 0.0011 0.0012 0.0007 0.0003 0.0027 0.0003 0.0014 0.0002 0.0004 0.0005 0.0004 0.0003 0.0003 0.0006 0.0005 0.0002
Bud Site 0.0157 0.0055 0.0115 0.0019 0.0011 0.0004 0.0046 0.002 0.0048 0.0006 0.0005 0.0005 0.0011 0.0009 0.0022 0.0004 0.0015 0.0004
Cell Periphery 0.0007 0.0007 0.002 0.0007 0.0002 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0004 0.0003 0.0003 0.0001 0.0002 0.0001
Cytoplasm 0.0023 0.0101 0.0029 0.0002 0.0004 0.0011 0.0086 0.0046 0.002 0.001 0.0002 0.0009 0.0044 0.0048 0.0016 0.0005 0.0003 0.0011
Cytoplasmic Foci 0.0109 0.0026 0.0062 0.0028 0.0104 0.0003 0.0119 0.0017 0.0088 0.0042 0.0033 0.002 0.0021 0.0006 0.0028 0.0012 0.0071 0.0006
Eisosomes 0.0015 0.0001 0.0002 0.0002 0.0002 0 0.0006 0 0.0002 0.0001 0.0001 0 0.0004 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0039 0.0017 0.0014 0.0005 0.0011 0.0008 0.005 0.0004 0.0018 0.0014 0.0001 0.0004 0.0078 0.0023 0.0005 0.0002 0.0001 0.0003
Endosome 0.0189 0.0054 0.007 0.0054 0.0059 0.0009 0.0355 0.0018 0.0149 0.0099 0.0072 0.0046 0.0101 0.0029 0.0017 0.0007 0.0016 0.0007
Golgi 0.008 0.0007 0.0035 0.0025 0.0066 0.0002 0.0123 0.0003 0.0043 0.0064 0.0066 0.0025 0.002 0.0003 0.0008 0.0003 0.0013 0.0002
Lipid Particles 0.0267 0.0054 0.0213 0.0169 0.021 0.0011 0.0247 0.0018 0.0189 0.0139 0.0148 0.0021 0.006 0.0033 0.0042 0.0036 0.0196 0.0018
Mitochondria 0.0055 0.0021 0.0021 0.0031 0.0013 0.0011 0.0398 0.0005 0.0053 0.0127 0.0249 0.0022 0.0024 0.0009 0.0006 0.0005 0.0006 0.0005
None 0.0056 0.0128 0.0018 0.0004 0.0006 0.0003 0.0099 0.0006 0.0006 0.0015 0.0004 0.0007 0.0192 0.0639 0.0026 0.0006 0.0004 0.0009
Nuclear Periphery 0.047 0.06 0.0514 0.0049 0.0057 0.045 0.067 0.0301 0.0548 0.0115 0.0115 0.0151 0.0171 0.0883 0.0678 0.0631 0.0031 0.0337
Nucleolus 0.1337 0.1843 0.3322 0.4826 0.2818 0.2096 0.1185 0.2074 0.2963 0.3929 0.2904 0.223 0.1727 0.1033 0.3512 0.4835 0.3705 0.1769
Nucleus 0.6159 0.5831 0.3799 0.1714 0.161 0.6124 0.4972 0.6671 0.4377 0.285 0.2239 0.6136 0.5917 0.5369 0.4023 0.2703 0.1464 0.6144
Peroxisomes 0.0069 0.0006 0.0029 0.002 0.0265 0.0001 0.0085 0.0006 0.004 0.0054 0.0015 0.0011 0.0033 0.0002 0.0058 0.0006 0.0038 0.0002
Punctate Nuclear 0.0353 0.1063 0.1447 0.2836 0.3674 0.1187 0.0965 0.0765 0.1145 0.2473 0.4051 0.1213 0.1302 0.1844 0.139 0.1699 0.3943 0.1617
Vacuole 0.0057 0.0112 0.0068 0.0031 0.0006 0.0009 0.0065 0.0017 0.0015 0.0009 0.0005 0.0007 0.0039 0.0027 0.0022 0.0003 0.0008 0.0007
Vacuole Periphery 0.003 0.003 0.0035 0.0008 0.0003 0.0009 0.0052 0.0007 0.0015 0.0009 0.0008 0.0004 0.001 0.0026 0.0007 0.0007 0.0001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.9936 17.1378 8.478 11.0199 14.7296 19.8702 24.9491 17.1097 13.2836 17.4591
Translational Efficiency 0.5842 0.8344 0.692 0.5137 0.551 0.4106 0.4853 0.3937 0.5015 0.4689

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘