Standard name
Human Ortholog
Description UBX domain-containing protein that interacts with Cdc48p; ubiquitin regulatory X is also known as UBX
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.97 0.99 0.96 0.96 0.86 0.8 0.7 0.75 0.76 0.55 0.67 0.98 1.0 0.98 – 0.93 0.94 0.74 0.81 0.66 0.83 0.79 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.08 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.13 0 0 0.1 0.07 0.27 0.32 0.35 0.38 0.43 0.61 0.5 0 0 0 – 0 0 0.14 0.06 0.18 0.08 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 3 5 6 1 2 4
Bud 1 0 2 0 3 1 2 10 10 8 7 9 1 0 0 – 0 0 1 9 1 1 3 4
Bud Neck 0 1 3 1 4 3 3 13 2 2 2 2 2 0 0 – 0 1 2 2 3 1 1 3
Bud Site 0 0 0 0 0 0 1 1 3 0 1 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 5 0 2 1 2 0 2 0 4 0 0 3 1 1 – 1 2 1 2 3 0 2 2
Cytoplasm 259 462 291 197 265 210 276 222 271 275 159 213 234 407 388 – 158 129 203 388 183 144 187 235
Endoplasmic Reticulum 1 5 1 0 0 0 0 2 1 0 5 0 1 0 2 – 14 10 2 0 2 0 0 2
Endosome 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 – 1 3 1 9 2 1 4 3
Golgi 0 1 0 1 0 0 0 0 0 1 0 0 0 0 4 – 1 1 2 1 0 0 1 0
Mitochondria 37 2 3 20 20 65 111 112 138 156 177 158 0 0 0 – 3 4 37 30 51 13 16 12
Nucleus 0 1 0 0 1 0 0 1 1 0 0 1 0 0 2 – 2 0 0 2 0 0 0 0
Nuclear Periphery 1 0 0 0 1 1 0 0 0 0 1 0 0 0 0 – 0 0 0 1 0 0 0 0
Nucleolus 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 – 2 1 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0
SpindlePole 1 0 1 3 1 1 8 6 12 7 5 9 0 0 0 – 0 2 5 11 2 2 4 3
Vac/Vac Membrane 0 2 1 0 5 0 1 0 1 11 1 0 0 0 0 – 11 4 5 5 7 1 2 5
Unique Cell Count 278 474 294 205 277 245 343 317 363 362 290 316 239 407 394 169 137 276 481 278 174 238 284
Labelled Cell Count 301 481 302 225 301 283 402 369 439 464 358 393 242 408 397 193 157 276 481 278 174 238 284


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.0 3.2 3.0 3.2 2.9 2.3 2.5 2.7 2.4 2.5 2.4 4.3 4.0 4.3 4.6 5.9 5.8 3.4 3.9 4.4
Std Deviation (1e-4) 0.7 1.0 0.9 1.0 1.4 1.2 1.0 0.8 1.2 0.9 0.8 1.2 1.2 1.1 1.4 1.6 1.9 2.3 1.0 1.6 1.1
Intensity Change (Log2) – – – -0.1 -0.02 -0.18 -0.47 -0.36 -0.26 -0.46 -0.39 -0.44 0.4 0.3 0.4 0.5 0.86 0.83 0.08 0.27 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0863 1.8472 1.6544 1.4164 1.4978 1.8636 2.3215 2.2827 2.23 2.6572 2.4509 1.9978 2.4757 2.3084 2.311 2.1529 2.4985 2.6331
Actin 0.021 0.0008 0.0086 0.0043 0.013 0.0079 0.0476 0.0018 0.0432 0.025 0.0313 0.0017 0.025 0.0006 0.0073 0 0.0266 0.0043
Bud 0.0018 0.0034 0.0022 0 0.0035 0.0009 0.0037 0.0073 0.0007 0.0116 0.0042 0.002 0.0004 0.0016 0.0008 0 0.0038 0.0028
Bud Neck 0.0085 0.0002 0.0001 0.0025 0.0007 0.0011 0.0047 0.0008 0.0023 0.0012 0.0077 0.0023 0.0096 0.0004 0.003 0 0.0051 0.001
Bud Periphery 0.0075 0.002 0.0008 0.0001 0.0138 0.0014 0.0165 0.007 0.001 0.0149 0.0077 0.0034 0.0008 0.0023 0.0019 0 0.0099 0.0056
Bud Site 0.0084 0.0107 0.0023 0.0003 0.0012 0.0003 0.0114 0.03 0.0144 0.0141 0.0075 0.0005 0.0054 0.0127 0.0013 0 0.0143 0.0021
Cell Periphery 0.0009 0.0004 0.0002 0.0001 0.0002 0.0002 0.0009 0.0003 0.0003 0.0033 0.0008 0.0001 0.0003 0.0005 0.0004 0 0.0018 0.0001
Cytoplasm 0.0244 0.0314 0.0239 0.0194 0.0445 0.038 0.0118 0.0099 0.0103 0.0074 0.0091 0.0198 0.0151 0.0236 0.0187 0.0455 0.0213 0.0217
Cytoplasmic Foci 0.0134 0.0071 0.0069 0.0027 0.0083 0.005 0.0095 0.0038 0.0067 0.0226 0.0074 0.0083 0.0232 0.0018 0.0068 0.0025 0.0036 0.0118
Eisosomes 0.0008 0.0002 0.0002 0.0004 0.0002 0.0001 0.0006 0.0002 0.001 0.0019 0.0004 0.0001 0.0006 0.0002 0.0003 0.0001 0.0004 0.0001
Endoplasmic Reticulum 0.0074 0.0006 0.0006 0.0003 0.0021 0.0005 0.0032 0.0002 0.0011 0.004 0.0007 0.0011 0.0022 0.0004 0.0004 0.0002 0.0006 0.0004
Endosome 0.0077 0.0028 0.0027 0.001 0.0087 0.0014 0.008 0.0001 0.0048 0.0044 0.0151 0.0019 0.0051 0.0003 0.0009 0.0027 0.0009 0.0009
Golgi 0.004 0.0002 0.0006 0.0004 0.0012 0.0005 0.0033 0.0001 0.0027 0.0035 0.0118 0.0006 0.0018 0.0002 0.0007 0.0001 0.0017 0.0002
Lipid Particles 0.0036 0.0003 0.0013 0.0008 0.0019 0.0004 0.0039 0.0002 0.0053 0.0071 0.0059 0.0006 0.0023 0.0001 0.0018 0.0001 0.0033 0.0005
Mitochondria 0.0062 0.0029 0.001 0.0003 0.0012 0.0009 0.0018 0.0008 0.0027 0.0038 0.0136 0.0007 0.0012 0.0014 0.0021 0.0001 0.0027 0.0018
None 0.8431 0.9157 0.9357 0.9518 0.8493 0.9383 0.8369 0.9226 0.877 0.8462 0.8392 0.9479 0.8708 0.9501 0.935 0.9472 0.8949 0.9374
Nuclear Periphery 0.0164 0.0019 0.0009 0.0008 0.0235 0.0006 0.0079 0.0003 0.0036 0.0017 0.001 0.0014 0.015 0.0002 0.0007 0.0001 0.0003 0.0036
Nucleolus 0.0029 0.0016 0.0005 0.0015 0.001 0.0002 0.0026 0.0019 0.0015 0.0012 0.0014 0.0011 0.001 0.0004 0.0011 0 0.0005 0.0006
Nucleus 0.0103 0.0048 0.0012 0.0014 0.007 0.0005 0.0094 0.0028 0.0022 0.0015 0.0016 0.0018 0.0043 0.0016 0.0063 0.0001 0.0008 0.001
Peroxisomes 0.0023 0.0003 0.006 0.0005 0.0036 0.0003 0.0067 0.0006 0.0065 0.0186 0.0054 0.0003 0.0071 0.0002 0.0052 0 0.0063 0.0013
Punctate Nuclear 0.006 0.0096 0.0036 0.0112 0.0125 0.001 0.007 0.0087 0.0118 0.0035 0.0045 0.0031 0.0076 0.0007 0.0046 0.0001 0.0005 0.0023
Vacuole 0.0027 0.002 0.0005 0.0001 0.0018 0.0003 0.0019 0.0005 0.0006 0.002 0.0223 0.001 0.0006 0.0006 0.0006 0.001 0.0006 0.0003
Vacuole Periphery 0.0008 0.001 0.0001 0.0001 0.0008 0.0001 0.0006 0.0001 0.0003 0.0005 0.0014 0.0003 0.0006 0.0001 0.0001 0.0002 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.9108 15.7505 15.5834 17.1155 12.3739 11.7397 18.9113 23.0645 18.7021 15.7292
Translational Efficiency 2.2624 1.641 1.7339 1.485 1.6226 2.4421 1.435 1.4554 1.7107 1.455

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
215 1279 1599 1749 1324 2095 1248 40 1539 3374 2847 1789

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 601.30 609.50 694.37 745.52 572.85 630.20 765.46 823.74 576.82 622.35 725.53 747.27
Standard Deviation 74.85 103.41 84.98 102.97 60.72 80.95 87.56 118.35 63.65 90.68 93.07 103.99
Intensity Change Log 2 — 0.019541 0.207620 0.310162 — 0.137652 0.418170 0.524032 — 0.078374 0.314184 0.418468

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000131 0.000305 0.000102 0.001208 0.000024 0.000457 0.000115 0.000281 0.000039 0.000399 0.000108 0.001187
Bud Neck 0.004300 0.044220 0.003405 0.015932 0.003729 0.065852 0.001735 0.003115 0.003808 0.057652 0.002673 0.015645
Bud Site 0.000816 0.015030 0.007928 0.046828 0.000627 0.020891 0.005364 0.005511 0.000654 0.018669 0.006804 0.045904
Cell Periphery 0.000176 0.000186 0.000864 0.000259 0.000097 0.000224 0.000176 0.000245 0.000108 0.000210 0.000562 0.000258
Cytoplasm 0.413271* 0.344903* 0.348578* 0.420222* 0.539799* 0.343125* 0.427954* 0.367946* 0.522123* 0.343799* 0.383373* 0.419053*
Cytoplasmic Foci 0.165453 0.288737* 0.015019 0.015760 0.110837 0.265918* 0.022003 0.094171 0.118467 0.274568* 0.018080 0.017513
Eisosomes 0.000186 0.000216 0.000130 0.000039 0.000068 0.000204 0.000054 0.000103 0.000085 0.000208 0.000097 0.000041
Endoplasmic Reticulum 0.000516 0.000552 0.000843 0.002803 0.000262 0.000591 0.001087 0.001248 0.000298 0.000576 0.000950 0.002768
Endosome 0.004480 0.031705 0.001329 0.007249 0.002670 0.031201 0.001989 0.027511 0.002922 0.031392 0.001619 0.007702
Golgi 0.002179 0.007029 0.000068 0.006029 0.000216 0.008420 0.000091 0.006192 0.000490 0.007893 0.000078 0.006033
Lipid Particles 0.011895 0.014382 0.001337 0.001166 0.004354 0.010309 0.001335 0.002325 0.005407 0.011853 0.001336 0.001191
Mitochondria 0.000951 0.019568 0.000648 0.006487 0.000936 0.024744 0.000550 0.018531 0.000938 0.022782 0.000605 0.006756
Mitotic Spindle 0.000132 0.005625 0.002783 0.040087 0.000087 0.010669 0.001611 0.082752 0.000094 0.008757 0.002269 0.041041
None 0.002557 0.009164 0.016124 0.007188 0.012055 0.004329 0.009186 0.005390 0.010728 0.006162 0.013083 0.007148
Nuclear Periphery 0.000717 0.000580 0.000908 0.002017 0.000430 0.000382 0.001015 0.000934 0.000470 0.000457 0.000955 0.001993
Nuclear Periphery Foci 0.000244 0.000521 0.000240 0.000758 0.000196 0.000273 0.000447 0.000669 0.000202 0.000367 0.000331 0.000756
Nucleolus 0.001470 0.005955 0.000922 0.000519 0.001600 0.003510 0.000609 0.000363 0.001582 0.004437 0.000785 0.000515
Nucleus 0.372540* 0.123194 0.579011* 0.340095* 0.301312* 0.110467 0.476682* 0.238440* 0.311263* 0.115291 0.534155* 0.337822*
Peroxisomes 0.003855 0.023656 0.000340 0.003441 0.001551 0.027421 0.000221 0.001454 0.001873 0.025994 0.000288 0.003397
Vacuole 0.013860 0.061497 0.019026 0.076240 0.018874 0.068550 0.047388 0.124723 0.018174 0.065877 0.031459 0.077324
Vacuole Periphery 0.000269 0.002976 0.000395 0.005674 0.000277 0.002464 0.000388 0.018096 0.000276 0.002658 0.000392 0.005952

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.92 0.09 -2.64 -1.94 -2.93 -6.23 -4.37 -3.17 1.25 -1.93 -7.76 -4.76 -3.42 -1.42 -2.94
Bud Neck -12.53 0.33 -4.17 10.80 -6.85 -22.48 3.55 -0.66 20.35 -2.14 -26.24 1.58 -7.26 20.35 -8.28
Bud Site -11.22 -4.35 -10.61 -3.87 -6.30 -15.38 -3.04 -1.82 1.88 -1.11 -19.17 -5.49 -10.93 -2.44 -7.58
Cell Periphery -0.24 -5.08 0.36 0.90 5.26 -5.15 -4.61 -1.97 1.72 -0.16 -5.98 -6.03 -3.85 1.96 5.05
Cytoplasm 3.80 4.20 4.14 0.46 -0.18 20.82 14.42 5.32 1.20 2.08 21.64 19.15 19.78 0.69 2.52
Cytoplasmic Foci -10.01 14.28 14.65 44.15 3.27 -24.34 22.40 4.34 14.59 -2.08 -28.81 27.05 28.15 66.16 4.46
Eisosomes -0.93 2.28 5.66 8.32 4.52 -13.77 2.32 -3.82 5.28 -4.48 -11.83 -0.97 7.32 16.19 4.71
Endoplasmic Reticulum -0.19 -2.73 -7.97 -9.44 -7.45 -3.93 -14.35 -4.99 -4.30 -2.09 -4.37 -15.25 -10.44 -9.49 -6.85
Endosome -11.89 2.34 0.72 14.60 -4.50 -18.05 2.13 -1.21 2.94 -1.31 -23.02 4.08 -1.33 21.60 -4.23
Golgi -3.19 1.65 0.10 4.91 -2.67 -12.52 4.23 -0.94 3.43 -1.02 -14.54 2.31 -2.07 6.49 -2.71
Lipid Particles -0.84 3.58 3.61 11.52 0.63 -8.00 8.25 3.71 10.34 -2.28 -8.20 7.84 7.97 17.84 0.71
Mitochondria -10.54 0.78 -3.66 9.20 -5.30 -15.40 3.72 -0.87 3.44 -0.96 -18.75 3.05 -4.77 15.67 -5.63
Mitotic Spindle -3.77 -3.34 -9.68 -6.45 -8.18 -6.81 -2.84 -1.47 -1.04 -1.38 -7.79 -4.41 -9.79 -5.77 -8.61
None -4.47 -7.54 -5.17 2.26 5.47 4.98 2.89 3.87 0.78 2.08 3.25 -0.51 3.79 0.61 5.33
Nuclear Periphery 0.98 -3.13 -17.95 -20.54 -19.27 1.07 -17.41 -5.53 -5.73 -1.37 0.25 -16.87 -24.32 -24.12 -18.74
Nuclear Periphery Foci -2.31 -0.77 -5.21 -2.48 -6.56 -1.47 -9.96 -2.93 -2.62 -1.35 -3.37 -7.96 -8.51 -5.43 -5.13
Nucleolus -3.92 1.15 1.90 5.08 2.45 -3.46 5.02 5.69 6.10 2.47 -5.25 4.06 5.03 7.48 1.86
Nucleus 13.59 -10.95 -2.18 -34.56 16.62 25.96 -18.15 0.92 -3.66 5.31 28.99 -27.78 -10.80 -40.07 14.90
Peroxisomes -8.45 2.28 1.53 12.59 -2.34 -17.41 3.50 2.06 18.49 -1.60 -20.52 4.02 0.87 20.72 -2.50
Vacuole -15.67 -5.50 -26.15 -14.97 -22.97 -19.61 -15.27 -6.84 -5.67 -5.34 -23.89 -14.12 -26.44 -16.03 -18.53
Vacuole Periphery -7.71 -0.50 -2.86 1.26 -2.59 -13.10 -0.13 -0.97 -0.56 -0.97 -14.28 -0.50 -3.00 0.80 -2.85
Standard name
Human Ortholog
Description UBX domain-containing protein that interacts with Cdc48p; ubiquitin regulatory X is also known as UBX
Localization
Cell Percentages cytoplasm (36%), nucleus (3%), mixed (41%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.0004)
nucleus - T/G1 (0.00038)
Subcompartmental Group N/A

Ubx5

Ubx5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubx5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available