Standard name
Human Ortholog
Description Swi2/Snf2-related ATPase; structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; relocalizes to the cytosol in response to hypoxia; chronological aging factor that mediates lifespan extension by dietary restriction
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0.06 0.1 0.07 0.09 0.1 0.1 0.11 – 0.05 0.07 0.11 0.17 0.17 – – 0.29 0 0 0 0 0.06 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0.12 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0.32 0.58 0.66 0.39 0.71 0.7 0.75 0.75 – 0.86 0.84 0.12 0 0.22 – – 0.12 0 0 0.06 0 0 0
Nucleus 0.92 0.69 0.61 0.64 0.71 0.57 0.5 0.45 0.43 – 0.47 0.46 0.82 0.78 0.71 – – 0.59 0.56 0.63 0.54 0.65 0.56 0.47
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 – – 0.06 0.19 0.08 0.11 0.12 0.11 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.07 0 0 0 0 0 – 0 0 0 0 0 – – 0.06 0 0 0 0 0 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0.12 0.11 0.14 0.15 0.09 0.15 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 – – 0 0 1 0 0 0 1
Bud 0 0 0 1 2 2 6 12 4 – 6 9 0 0 5 – – 1 0 5 0 2 6 7
Bud Neck 1 0 1 0 1 0 2 1 1 – 2 0 0 0 0 – – 0 1 2 3 1 1 3
Bud Site 6 0 0 4 4 7 17 12 8 – 17 10 0 0 3 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 2 30 9 13 16 20 37 40 20 – 15 19 16 45 52 – – 5 1 7 3 1 16 6
Endoplasmic Reticulum 0 2 1 0 0 0 1 0 0 – 0 0 0 1 0 – – 1 0 1 0 2 0 1
Endosome 0 2 0 1 4 0 0 0 0 – 0 0 0 1 0 – – 2 10 11 4 2 2 7
Golgi 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 1 3 0 0 0 2
Mitochondria 6 107 93 86 92 165 268 304 139 – 242 227 18 8 68 – – 2 2 8 9 1 7 7
Nucleus 167 228 97 84 165 132 192 182 79 – 132 123 122 211 218 – – 10 119 243 90 107 152 143
Nuclear Periphery 0 2 0 0 1 0 2 1 0 – 1 0 0 0 0 – – 0 1 2 0 0 0 2
Nucleolus 15 11 1 1 7 3 5 8 4 – 4 10 4 8 15 – – 1 40 30 17 20 29 54
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 1
SpindlePole 4 2 9 9 10 3 16 10 3 – 4 4 2 5 1 – – 1 7 10 7 7 11 25
Vac/Vac Membrane 1 12 0 1 2 4 1 5 2 – 2 8 3 9 8 – – 2 24 53 24 15 39 37
Unique Cell Count 182 331 159 131 233 232 381 408 185 282 269 148 270 307 17 215 386 168 166 272 308
Labelled Cell Count 202 397 211 200 304 336 547 576 260 425 410 165 288 370 25 215 386 168 166 272 308


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.1 3.5 3.7 3.8 3.3 3.2 3.1 2.8 – 2.8 2.6 4.9 5.1 4.2 – – 6.3 4.9 4.2 5.0
Std Deviation (1e-4) 0.8 1.0 1.0 1.8 1.6 1.1 1.2 1.0 0.9 – 0.9 0.9 1.1 1.2 0.9 – – 2.1 1.5 1.0 1.3
Intensity Change (Log2) – – – 0.09 0.13 -0.1 -0.13 -0.16 -0.34 – -0.31 -0.41 0.48 0.54 0.27 – – 0.83 0.47 0.27 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1327 3.5325 3.3612 2.7862 3.3623 3.3349 1.4143 2.6574 2.3678 1.9886 1.819 2.5647 2.0331 2.4952 1.6238 1.7792 1.7317 1.6232
Actin 0.0126 0 0.0009 0.0041 0.0079 0.0009 0.0038 0.0001 0.0142 0.0002 0.0013 0.0003 0.0113 0.001 0.0066 0.0288 0.0025 0.0001
Bud 0.0004 0.0003 0.0001 0.0001 0.0009 0.0001 0.0006 0.0009 0.0006 0.0004 0.0012 0.0003 0.0001 0.0001 0.0001 0.0001 0.0022 0
Bud Neck 0.0014 0.0005 0.0004 0.0004 0.0059 0.0035 0.0025 0.0009 0.0002 0.0005 0.003 0.0075 0.0003 0.0001 0.0003 0.0005 0.0038 0.0018
Bud Periphery 0.0009 0.0002 0.0003 0.0001 0.0044 0.0002 0.0014 0.0002 0.0006 0.001 0.0047 0.0011 0.0001 0.0001 0.0001 0.0001 0.0092 0
Bud Site 0.002 0.0016 0.0013 0.0005 0.0024 0.0005 0.0012 0.0012 0.0093 0.0009 0.0014 0.0006 0.0006 0.0005 0.0011 0.0006 0.0045 0.0001
Cell Periphery 0.0004 0 0.0003 0.0001 0.0021 0 0.0005 0.0001 0.0001 0.0001 0.0005 0.0001 0.0001 0.0001 0.0001 0 0.0017 0
Cytoplasm 0.0019 0.0004 0.0105 0.0083 0.0039 0.0002 0.0157 0.0036 0.0006 0.0004 0.0009 0.0005 0.0078 0.0013 0.0065 0.0035 0.005 0.0003
Cytoplasmic Foci 0.002 0 0.0003 0.0102 0.0006 0.0021 0.0185 0.0159 0.0105 0.0014 0.0002 0.0002 0.0083 0.0025 0.0132 0.0118 0.0078 0
Eisosomes 0.0002 0 0.0001 0 0.0005 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0.0001 0.0001 0.0003
Endoplasmic Reticulum 0.0051 0 0.001 0.0022 0.005 0 0.0023 0.0003 0.0003 0.0001 0.0006 0.0001 0.0022 0.0041 0.0012 0.0002 0.0011 0
Endosome 0.01 0.0001 0.0032 0.0288 0.0079 0.0009 0.0116 0.0011 0.0045 0.0041 0.0023 0.0004 0.0185 0.0193 0.0079 0.0032 0.0133 0.0001
Golgi 0.0045 0 0.0011 0.0041 0.0019 0.0008 0.0012 0.0004 0.0055 0.0014 0.0003 0.0002 0.0035 0.0028 0.0043 0.0092 0.0087 0
Lipid Particles 0.0055 0 0.0007 0.0183 0.005 0.0005 0.0013 0.0023 0.0024 0.0022 0.0004 0.0011 0.0136 0.0109 0.011 0.0005 0.0242 0.0001
Mitochondria 0.0162 0.0002 0.0036 0.0009 0.0444 0.0004 0.003 0.0006 0.0048 0.0053 0.0064 0.0011 0.0009 0.0036 0.0015 0.0038 0.0103 0.0001
None 0.0026 0.0007 0.0018 0.0048 0.0014 0.0001 0.004 0.003 0.0003 0.0003 0.0006 0.0002 0.0256 0.0004 0.0384 0.005 0.0011 0.0053
Nuclear Periphery 0.0059 0.0013 0.0022 0.0038 0.0336 0.0004 0.0116 0.0021 0.0009 0.0027 0.019 0.0047 0.0409 0.0058 0.0081 0.0044 0.0041 0.0008
Nucleolus 0.0408 0.0545 0.0372 0.0337 0.0521 0.0779 0.0542 0.0285 0.0252 0.0305 0.0608 0.086 0.0224 0.0218 0.0173 0.0194 0.0586 0.0542
Nucleus 0.8692 0.9364 0.9301 0.8251 0.7905 0.8863 0.8248 0.9178 0.9133 0.9412 0.8822 0.8844 0.8197 0.9206 0.8646 0.8927 0.7962 0.9274
Peroxisomes 0.0032 0 0.0001 0.0198 0.0013 0.0023 0.0196 0.0039 0.0048 0.0022 0.0002 0.0008 0.0088 0.0008 0.0056 0.011 0.01 0
Punctate Nuclear 0.008 0.0035 0.004 0.0325 0.0072 0.0228 0.0117 0.0167 0.0016 0.003 0.0068 0.0094 0.0116 0.0024 0.0114 0.0051 0.0188 0.0093
Vacuole 0.0046 0.0003 0.0006 0.0017 0.0154 0.0002 0.0085 0.0004 0.0002 0.0013 0.0047 0.0009 0.0017 0.0012 0.0005 0.0001 0.0148 0.0001
Vacuole Periphery 0.0026 0 0.0003 0.0006 0.0055 0 0.0021 0.0001 0.0001 0.0009 0.0025 0.0002 0.0018 0.0007 0.0002 0.0001 0.0022 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.6646 17.796 24.6394 24.8864 18.5712 22.8081 25.6188 29.1022 29.1453 23.0907
Translational Efficiency 0.3264 0.3388 0.2344 0.256 0.2067 0.2859 0.2767 0.2124 0.1963 0.2284

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
240 468 76 222 1718 2198 1914 56 1958 2666 1990 278

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 635.79 724.77 1034.55 905.21 629.34 741.96 894.68 933.58 630.13 738.94 900.02 910.92
Standard Deviation 80.01 158.13 130.54 123.38 93.80 100.38 110.12 123.65 92.24 112.87 114.16 123.96
Intensity Change Log 2 — 0.188973 0.702381 0.509702 — 0.237502 0.507532 0.568934 — 0.213318 0.608740 0.539471

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000078 0.001201 0.004018 0.001628 0.000229 0.000297 0.000771 0.002346 0.000210 0.000455 0.000895 0.001772
Bud Neck 0.002271 0.011704 0.005960 0.018320 0.005652 0.009261 0.018743 0.018930 0.005238 0.009690 0.018254 0.018443
Bud Site 0.000487 0.030386 0.012539 0.022153 0.001308 0.002951 0.005558 0.008884 0.001207 0.007767 0.005824 0.019480
Cell Periphery 0.000157 0.000444 0.000148 0.000298 0.000154 0.000090 0.000094 0.000143 0.000154 0.000152 0.000096 0.000267
Cytoplasm 0.132489* 0.078723 0.006753 0.139475* 0.104266* 0.038058 0.019421 0.024373 0.107725* 0.045197 0.018937 0.116289*
Cytoplasmic Foci 0.040380 0.028565 0.000806 0.029933 0.016142 0.008258 0.001617 0.000458 0.019113 0.011823 0.001586 0.023995
Eisosomes 0.000056 0.000078 0.000096 0.000071 0.000035 0.000032 0.000030 0.000071 0.000037 0.000040 0.000033 0.000071
Endoplasmic Reticulum 0.000986 0.006758 0.014899 0.007207 0.001493 0.003031 0.004812 0.013584 0.001431 0.003685 0.005197 0.008492
Endosome 0.000925 0.004877 0.000934 0.044946 0.001320 0.001660 0.002989 0.001349 0.001272 0.002225 0.002911 0.036164
Golgi 0.000177 0.002949 0.000266 0.026697 0.000315 0.001143 0.001619 0.000233 0.000298 0.001460 0.001568 0.021366
Lipid Particles 0.005029 0.006915 0.001346 0.001714 0.002818 0.004237 0.000341 0.000880 0.003089 0.004707 0.000379 0.001546
Mitochondria 0.001291 0.014381 0.001716 0.004371 0.003688 0.011221 0.004087 0.002236 0.003394 0.011776 0.003996 0.003941
Mitotic Spindle 0.000213 0.022663 0.002107 0.089418 0.002605 0.009358 0.011859 0.011701 0.002312 0.011694 0.011486 0.073763
None 0.017957 0.029839 0.002180 0.016385 0.032723 0.007680 0.006869 0.006863 0.030913 0.011570 0.006690 0.014467
Nuclear Periphery 0.001548 0.002443 0.000981 0.002200 0.002343 0.006751 0.001927 0.001711 0.002245 0.005995 0.001891 0.002101
Nuclear Periphery Foci 0.000784 0.004124 0.001124 0.001533 0.000852 0.001563 0.000401 0.000595 0.000844 0.002012 0.000428 0.001344
Nucleolus 0.014513 0.030717 0.015175 0.005472 0.024619 0.030033 0.016396 0.012674 0.023380 0.030153 0.016349 0.006923
Nucleus 0.776345* 0.706731* 0.919391* 0.531182* 0.796068* 0.857215* 0.895347* 0.877448* 0.793650* 0.830798* 0.896265* 0.600933*
Peroxisomes 0.001441 0.007762 0.001197 0.001880 0.001730 0.002945 0.000406 0.001071 0.001695 0.003791 0.000436 0.001717
Vacuole 0.002689 0.007532 0.008053 0.052062 0.001349 0.003239 0.005686 0.013969 0.001513 0.003993 0.005776 0.044388
Vacuole Periphery 0.000184 0.001209 0.000312 0.003057 0.000292 0.000976 0.001030 0.000481 0.000278 0.001017 0.001003 0.002538

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.02 -3.68 -8.65 -4.00 1.45 -1.15 -10.55 -4.74 -4.65 -3.76 -3.54 -11.53 -9.15 -8.34 -5.74
Bud Neck -5.38 -4.79 -8.04 -2.68 -4.96 -4.41 -18.76 -4.81 -4.24 -1.38 -6.02 -19.34 -8.23 -6.00 1.46
Bud Site -4.66 -1.36 -4.75 1.94 -0.25 -2.36 -5.79 -3.07 -2.82 -2.49 -5.11 -5.93 -5.42 -3.01 -3.74
Cell Periphery -3.08 -0.93 -2.22 2.29 -0.96 4.14 4.70 -2.44 -3.80 -3.86 0.30 4.93 -3.31 -3.22 -6.64
Cytoplasm 3.78 9.21 3.33 -0.12 -6.65 13.76 19.78 14.10 4.41 -0.52 13.52 21.56 4.63 -2.42 -6.20
Cytoplasmic Foci 1.61 5.77 4.59 4.57 -2.48 5.44 11.58 11.80 10.47 0.58 4.63 12.74 5.87 2.92 -2.46
Eisosomes -3.17 -3.60 -5.93 -3.35 -1.08 0.97 -2.23 -4.84 -4.96 -4.57 -1.57 -2.10 -10.37 -9.87 -9.73
Endoplasmic Reticulum -6.34 -10.80 -9.47 -5.03 0.16 -7.88 -18.36 -7.51 -6.88 -5.61 -9.86 -20.22 -11.37 -9.57 -7.41
Endosome -3.43 -1.11 -2.34 -1.33 -2.18 -1.00 -0.73 -2.38 -1.85 -1.48 -2.57 -0.86 -2.34 -2.04 -2.20
Golgi -3.38 -2.53 -1.82 -0.71 -1.75 -2.05 -1.42 -0.96 1.78 1.07 -3.22 -1.49 -1.77 -1.20 -1.55
Lipid Particles -1.61 4.94 3.94 5.25 -1.88 -2.75 11.92 3.81 4.81 -4.86 -3.44 13.35 3.50 5.44 -6.75
Mitochondria -4.22 -1.92 -3.64 3.63 -2.13 -5.93 0.81 1.83 6.99 0.90 -7.02 0.46 1.02 7.60 0.51
Mitotic Spindle -4.04 -1.29 -5.83 -3.93 -4.49 -4.33 -7.40 -2.29 -1.87 -1.22 -5.75 -7.64 -6.07 -5.22 -4.49
None -2.41 6.54 2.42 4.42 -5.60 10.21 11.25 9.77 1.50 0.36 8.62 11.88 8.50 0.96 -3.15
Nuclear Periphery -1.65 0.59 -4.69 -3.52 -5.90 -7.38 -0.13 -2.17 3.81 -1.96 -7.43 -0.29 -4.63 1.84 -4.07
Nuclear Periphery Foci -2.83 -1.43 -2.62 1.45 -1.04 -1.78 2.42 -1.26 -0.07 -2.08 -3.15 2.07 -3.49 -0.63 -4.45
Nucleolus -4.78 -0.21 4.15 8.54 2.97 -2.43 1.61 2.00 2.91 1.42 -3.58 1.14 9.48 12.93 8.18
Nucleus 2.79 -5.13 5.12 2.89 9.63 -7.58 -11.05 1.60 3.50 4.27 -4.84 -12.08 7.05 9.12 11.89
Peroxisomes -4.19 1.06 2.36 4.73 1.32 -2.59 5.12 2.11 4.35 -2.51 -4.35 5.50 3.38 6.98 -2.92
Vacuole -2.29 -2.88 -8.19 -7.82 -7.21 -5.26 -6.85 -3.69 -3.57 -3.39 -5.94 -6.91 -9.15 -8.88 -8.54
Vacuole Periphery -5.19 -4.10 -3.03 0.97 -2.16 -4.41 -1.34 -2.85 2.85 0.15 -5.50 -1.44 -2.84 0.95 -1.12
Standard name
Human Ortholog
Description Swi2/Snf2-related ATPase; structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; relocalizes to the cytosol in response to hypoxia; chronological aging factor that mediates lifespan extension by dietary restriction
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Swr1

Swr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available