Standard name
Description Karyopherin; involved in nuclear import and export of proteins, including import of replication protein A and export of Far1p and transcription factors Swi5p, Swi6p, Msn2p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm; exportin-5 homolog
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0.12 0.11 0.12 0.08 0.08 0.12 – 0.08 0.07 0.13 0.23 0.32 0.21 0.23 0.29 0 0 0 0.08 0.09 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.14 0 0.17 0.25 0.26 0.48 – 0.46 0.54 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.56 0.6 0.64 0.54 0.32 0.42 0.47 0.49 0.43 – 0.45 0.42 0.35 0.17 0.09 0.35 0.31 0.27 0.57 0.77 0.63 0.58 0.48 0.36
Nuclear Periphery 0.05 0 0 0 0 0 0.06 0 0.05 – 0 0 0 0 0 0 0 0 0.2 0 0.09 0 0 0.05
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.47 0.58 0.35 0.32 0.59 0.39 0.31 0.29 0.2 – 0.23 0.23 0.67 0.7 0.64 0.47 0.42 0.5 0.09 0.11 0.17 0.16 0.26 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 1 0 2 1 0 0 1 0 0 1 0 0
Bud 0 0 0 0 0 2 2 4 5 – 7 5 1 0 2 2 0 1 5 6 3 14 31 26
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 1 4 3 0 – 3 7 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 1 2 1 1 0 0 0 0 0 0 0
Cytoplasm 14 12 10 23 27 34 31 39 29 – 23 28 47 106 148 51 25 47 2 2 3 21 39 34
Endoplasmic Reticulum 2 0 0 0 1 2 0 1 0 – 0 1 1 4 0 7 5 8 2 0 1 0 4 6
Endosome 1 0 0 0 4 4 3 4 1 – 0 0 1 2 3 5 3 2 4 1 2 6 10 26
Golgi 1 0 0 0 0 0 0 0 0 – 0 0 0 0 1 3 2 1 5 2 1 1 0 1
Mitochondria 2 4 8 26 10 49 95 132 117 – 136 220 0 1 13 10 5 3 0 2 2 1 3 5
Nucleus 170 188 123 102 79 118 181 251 104 – 131 171 129 78 39 85 33 44 191 259 141 157 210 179
Nuclear Periphery 16 1 3 2 9 7 23 19 11 – 12 11 2 1 1 2 0 3 66 10 21 7 8 23
Nucleolus 0 0 0 0 2 1 3 4 3 – 4 8 0 0 1 3 3 5 21 11 5 7 6 9
Peroxisomes 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 2 1 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 1 0 0 2 – 1 0 0 1 0 1 1 0 4 0 2 3 8 11
Vac/Vac Membrane 143 184 68 60 147 110 121 148 48 – 67 94 249 318 295 115 45 80 30 36 37 42 114 163
Unique Cell Count 304 315 192 189 248 281 386 517 244 293 408 370 457 458 245 107 161 339 336 226 272 441 500
Labelled Cell Count 349 389 212 213 279 329 463 606 320 384 545 431 512 507 288 124 195 339 336 226 272 441 500


Cytoplasm, Nuclear Periphery, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 8.4 6.1 6.0 6.5 6.0 5.8 5.8 5.3 – 5.1 4.9 9.0 8.9 7.7 10.7 12.2 11.7 8.3 7.7 8.8
Std Deviation (1e-4) 1.4 1.9 1.0 1.1 1.0 1.3 1.1 1.1 1.7 – 1.4 1.1 1.7 1.7 1.5 3.1 4.1 3.9 3.1 1.6 1.8
Intensity Change (Log2) – – – -0.04 0.1 -0.02 -0.09 -0.09 -0.2 – -0.27 -0.32 0.56 0.54 0.33 0.81 1.0 0.94 0.44 0.34 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 2.4 2.1 2.5 1.2 1.1 2.4 – 1.1 0.8 2.8 5.5 7.4 4.7 4.7 6.1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 3.3 -0.1 4.4 6.0 6.4 10.0 – 10.0 11.7 0 0 -0.9 0 0 -1.2
Nucleus -2.0 -6.7 -4.7 -3.9 -3.7 -4.4 – -4.2 -5.1 -6.6 -11.8 -14.9 -6.1 -5.5 -6.9
Nuclear Periphery 0 1.3 0 2.4 1.4 1.7 – 1.6 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole -0.8 5.0 0.8 -1.0 -1.7 -3.7 – -3.0 -3.2 7.2 8.1 6.8 2.4 1.1 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.423 11.1566 10.193 9.7747 9.1383 10.4142 5.7968 8.6457 7.6627 6.7733 6.4869 7.5835 6.4067 7.0735 6.5341 6.3826 6.1193 6.5533
Actin 0.0003 0.0002 0.0007 0.0002 0.0012 0.0005 0.0761 0.0002 0.0081 0.0424 0.0144 0.0045 0.0042 0.0002 0.0033 0.0004 0.0005 0.0003
Bud 0 0 0.0001 0 0 0.0001 0.0008 0 0.0002 0.0004 0.0006 0.0001 0 0 0.0001 0 0 0
Bud Neck 0.0002 0.0004 0.0004 0.001 0.0018 0.0035 0.0121 0.0001 0.0006 0.0118 0.0014 0.0013 0.0002 0.0002 0.0002 0.0002 0.0005 0.0019
Bud Periphery 0 0 0.0001 0 0.0001 0.0002 0.0011 0 0.0002 0.0006 0.0008 0.0003 0 0 0 0 0 0
Bud Site 0.0001 0.0002 0.0038 0 0.0001 0.0001 0.0055 0.0001 0.0054 0.0034 0.0052 0.0001 0.0001 0.0001 0.0007 0 0 0
Cell Periphery 0 0.0001 0.0001 0 0 0.0001 0.0004 0 0.0002 0.0004 0.0002 0 0 0 0 0 0 0
Cytoplasm 0.007 0.045 0.0131 0.0289 0.0032 0.0033 0.0158 0.005 0.0055 0.0215 0.0066 0.002 0.0042 0.0091 0.0042 0.0038 0.0016 0.0025
Cytoplasmic Foci 0 0.0002 0.0001 0.0001 0.0001 0 0.0259 0 0.005 0.0023 0.0346 0.0009 0.0043 0 0.0002 0 0 0
Eisosomes 0 0 0.0001 0 0 0 0.0005 0 0.0001 0.0005 0.0002 0.0001 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0042 0.0066 0.0012 0.002 0.0029 0.0039 0.0062 0.0013 0.003 0.0108 0.0028 0.003 0.0024 0.0016 0.0014 0.0033 0.0015 0.0015
Endosome 0.0008 0.0011 0.0003 0.0005 0.0019 0.0006 0.0305 0.0009 0.0045 0.0095 0.0259 0.0061 0.005 0.0003 0.0006 0.0005 0.0005 0.0003
Golgi 0 0 0.0001 0 0.0001 0 0.0145 0 0.0056 0.0038 0.0188 0.0054 0.0009 0 0.0002 0 0 0
Lipid Particles 0 0 0.0001 0 0.0001 0 0.0182 0 0.005 0.0027 0.0308 0.0044 0.0024 0 0.0003 0 0 0
Mitochondria 0.0004 0.0004 0.0019 0.0003 0.0012 0.0006 0.0119 0.0005 0.0045 0.0041 0.0168 0.0099 0.0005 0.0002 0.0004 0.0004 0.0008 0.0003
None 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0267 0.0001 0.0087 0.0042 0.0066 0.0002 0.0017 0.0001 0.0006 0.0002 0.0001 0.0001
Nuclear Periphery 0.2292 0.1545 0.1715 0.159 0.1523 0.1389 0.2247 0.1975 0.2077 0.1879 0.2559 0.1654 0.2637 0.2268 0.2137 0.2947 0.2728 0.1656
Nucleolus 0.001 0.0012 0.0009 0.0011 0.0035 0.005 0.0023 0.0012 0.0007 0.0011 0.0028 0.0019 0.0009 0.0011 0.0007 0.0005 0.0033 0.0019
Nucleus 0.7524 0.7849 0.8028 0.8028 0.8262 0.8383 0.4873 0.7887 0.7284 0.6852 0.5425 0.7886 0.7028 0.7569 0.7675 0.6631 0.7117 0.8224
Peroxisomes 0 0 0.0001 0 0 0 0.0106 0 0.0022 0.0012 0.0223 0.0007 0.0014 0 0.0002 0 0 0
Punctate Nuclear 0.001 0.0012 0.0004 0.0023 0.0026 0.0017 0.0216 0.0006 0.0011 0.0024 0.0041 0.0009 0.0027 0.0016 0.0042 0.0311 0.0039 0.0014
Vacuole 0.0014 0.0026 0.001 0.0008 0.0011 0.0018 0.0032 0.0013 0.0011 0.0021 0.0025 0.0012 0.001 0.0009 0.0005 0.0004 0.0006 0.0008
Vacuole Periphery 0.0016 0.0011 0.0011 0.0007 0.0013 0.0013 0.0041 0.0023 0.0022 0.002 0.0042 0.003 0.0013 0.0009 0.0008 0.0015 0.0018 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.2305 33.0134 39.634 45.5329 29.7801 39.9106 39.7573 41.0287 45.0323 42.3328
Translational Efficiency 0.8717 0.6929 0.6 0.6376 0.6781 0.7558 0.6264 0.5724 0.5541 0.4854

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘