Standard name
Human Ortholog
Description Karyopherin; involved in nuclear import and export of proteins, including import of replication protein A and export of Far1p and transcription factors Swi5p, Swi6p, Msn2p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm; exportin-5 homolog

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0.12 0.11 0.12 0.08 0.08 0.12 0.08 0.07 0.13 0.23 0.32 0.21 0.23 0.29 0 0 0 0.08 0.09 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.14 0 0.17 0.25 0.26 0.48 0.46 0.54 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.56 0.6 0.64 0.54 0.32 0.42 0.47 0.49 0.43 0.45 0.42 0.35 0.17 0.09 0.35 0.31 0.27 0.57 0.77 0.63 0.58 0.48 0.36
Nuclear Periphery 0.05 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0.2 0 0.09 0 0 0.05
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.47 0.58 0.35 0.32 0.59 0.39 0.31 0.29 0.2 0.23 0.23 0.67 0.7 0.64 0.47 0.42 0.5 0.09 0.11 0.17 0.16 0.26 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 0 0 1 0 0 1 0 0
Bud 0 0 0 0 0 2 2 4 5 7 5 1 0 2 2 0 1 5 6 3 14 31 26
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 1 4 3 0 3 7 0 0 0 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 1 0 0 0 0 0 0 0
Cytoplasm 14 12 10 23 27 34 31 39 29 23 28 47 106 148 51 25 47 2 2 3 21 39 34
Endoplasmic Reticulum 2 0 0 0 1 2 0 1 0 0 1 1 4 0 7 5 8 2 0 1 0 4 6
Endosome 1 0 0 0 4 4 3 4 1 0 0 1 2 3 5 3 2 4 1 2 6 10 26
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2 1 5 2 1 1 0 1
Mitochondria 2 4 8 26 10 49 95 132 117 136 220 0 1 13 10 5 3 0 2 2 1 3 5
Nucleus 170 188 123 102 79 118 181 251 104 131 171 129 78 39 85 33 44 191 259 141 157 210 179
Nuclear Periphery 16 1 3 2 9 7 23 19 11 12 11 2 1 1 2 0 3 66 10 21 7 8 23
Nucleolus 0 0 0 0 2 1 3 4 3 4 8 0 0 1 3 3 5 21 11 5 7 6 9
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 1 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 1 0 0 2 1 0 0 1 0 1 1 0 4 0 2 3 8 11
Vac/Vac Membrane 143 184 68 60 147 110 121 148 48 67 94 249 318 295 115 45 80 30 36 37 42 114 163
Unique Cell Count 304 315 192 189 248 281 386 517 244 293 408 370 457 458 245 107 161 339 336 226 272 441 500
Labelled Cell Count 349 389 212 213 279 329 463 606 320 384 545 431 512 507 288 124 195 339 336 226 272 441 500


Cytoplasm, Nuclear Periphery, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 8.4 6.1 6.0 6.5 6.0 5.8 5.8 5.3 5.1 4.9 9.0 8.9 7.7 10.7 12.2 11.7 8.3 7.7 8.8
Std Deviation (1e-4) 1.4 1.9 1.0 1.1 1.0 1.3 1.1 1.1 1.7 1.4 1.1 1.7 1.7 1.5 3.1 4.1 3.9 3.1 1.6 1.8
Intensity Change (Log2) -0.04 0.1 -0.02 -0.09 -0.09 -0.2 -0.27 -0.32 0.56 0.54 0.33 0.81 1.0 0.94 0.44 0.34 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 2.1 2.5 1.2 1.1 2.4 1.1 0.8 2.8 5.5 7.4 4.7 4.7 6.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 -0.1 4.4 6.0 6.4 10.0 10.0 11.7 0 0 -0.9 0 0 -1.2
Nucleus -2.0 -6.7 -4.7 -3.9 -3.7 -4.4 -4.2 -5.1 -6.6 -11.8 -14.9 -6.1 -5.5 -6.9
Nuclear Periphery 0 1.3 0 2.4 1.4 1.7 1.6 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.8 5.0 0.8 -1.0 -1.7 -3.7 -3.0 -3.2 7.2 8.1 6.8 2.4 1.1 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.423 11.1566 10.193 9.7747 9.1383 10.4142 5.7968 8.6457 7.6627 6.7733 6.4869 7.5835 6.4067 7.0735 6.5341 6.3826 6.1193 6.5533
Actin 0.0003 0.0002 0.0007 0.0002 0.0012 0.0005 0.0761 0.0002 0.0081 0.0424 0.0144 0.0045 0.0042 0.0002 0.0033 0.0004 0.0005 0.0003
Bud 0 0 0.0001 0 0 0.0001 0.0008 0 0.0002 0.0004 0.0006 0.0001 0 0 0.0001 0 0 0
Bud Neck 0.0002 0.0004 0.0004 0.001 0.0018 0.0035 0.0121 0.0001 0.0006 0.0118 0.0014 0.0013 0.0002 0.0002 0.0002 0.0002 0.0005 0.0019
Bud Periphery 0 0 0.0001 0 0.0001 0.0002 0.0011 0 0.0002 0.0006 0.0008 0.0003 0 0 0 0 0 0
Bud Site 0.0001 0.0002 0.0038 0 0.0001 0.0001 0.0055 0.0001 0.0054 0.0034 0.0052 0.0001 0.0001 0.0001 0.0007 0 0 0
Cell Periphery 0 0.0001 0.0001 0 0 0.0001 0.0004 0 0.0002 0.0004 0.0002 0 0 0 0 0 0 0
Cytoplasm 0.007 0.045 0.0131 0.0289 0.0032 0.0033 0.0158 0.005 0.0055 0.0215 0.0066 0.002 0.0042 0.0091 0.0042 0.0038 0.0016 0.0025
Cytoplasmic Foci 0 0.0002 0.0001 0.0001 0.0001 0 0.0259 0 0.005 0.0023 0.0346 0.0009 0.0043 0 0.0002 0 0 0
Eisosomes 0 0 0.0001 0 0 0 0.0005 0 0.0001 0.0005 0.0002 0.0001 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0042 0.0066 0.0012 0.002 0.0029 0.0039 0.0062 0.0013 0.003 0.0108 0.0028 0.003 0.0024 0.0016 0.0014 0.0033 0.0015 0.0015
Endosome 0.0008 0.0011 0.0003 0.0005 0.0019 0.0006 0.0305 0.0009 0.0045 0.0095 0.0259 0.0061 0.005 0.0003 0.0006 0.0005 0.0005 0.0003
Golgi 0 0 0.0001 0 0.0001 0 0.0145 0 0.0056 0.0038 0.0188 0.0054 0.0009 0 0.0002 0 0 0
Lipid Particles 0 0 0.0001 0 0.0001 0 0.0182 0 0.005 0.0027 0.0308 0.0044 0.0024 0 0.0003 0 0 0
Mitochondria 0.0004 0.0004 0.0019 0.0003 0.0012 0.0006 0.0119 0.0005 0.0045 0.0041 0.0168 0.0099 0.0005 0.0002 0.0004 0.0004 0.0008 0.0003
None 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0267 0.0001 0.0087 0.0042 0.0066 0.0002 0.0017 0.0001 0.0006 0.0002 0.0001 0.0001
Nuclear Periphery 0.2292 0.1545 0.1715 0.159 0.1523 0.1389 0.2247 0.1975 0.2077 0.1879 0.2559 0.1654 0.2637 0.2268 0.2137 0.2947 0.2728 0.1656
Nucleolus 0.001 0.0012 0.0009 0.0011 0.0035 0.005 0.0023 0.0012 0.0007 0.0011 0.0028 0.0019 0.0009 0.0011 0.0007 0.0005 0.0033 0.0019
Nucleus 0.7524 0.7849 0.8028 0.8028 0.8262 0.8383 0.4873 0.7887 0.7284 0.6852 0.5425 0.7886 0.7028 0.7569 0.7675 0.6631 0.7117 0.8224
Peroxisomes 0 0 0.0001 0 0 0 0.0106 0 0.0022 0.0012 0.0223 0.0007 0.0014 0 0.0002 0 0 0
Punctate Nuclear 0.001 0.0012 0.0004 0.0023 0.0026 0.0017 0.0216 0.0006 0.0011 0.0024 0.0041 0.0009 0.0027 0.0016 0.0042 0.0311 0.0039 0.0014
Vacuole 0.0014 0.0026 0.001 0.0008 0.0011 0.0018 0.0032 0.0013 0.0011 0.0021 0.0025 0.0012 0.001 0.0009 0.0005 0.0004 0.0006 0.0008
Vacuole Periphery 0.0016 0.0011 0.0011 0.0007 0.0013 0.0013 0.0041 0.0023 0.0022 0.002 0.0042 0.003 0.0013 0.0009 0.0008 0.0015 0.0018 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.2305 33.0134 39.634 45.5329 29.7801 39.9106 39.7573 41.0287 45.0323 42.3328
Translational Efficiency 0.8717 0.6929 0.6 0.6376 0.6781 0.7558 0.6264 0.5724 0.5541 0.4854

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1468 321 162 1327 1957 1947 214 1165 3425 2268 376 2492

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 719.52 931.76 1406.24 1207.54 790.37 951.15 1281.99 1207.56 760.00 948.41 1335.52 1207.55
Standard Deviation 96.83 162.14 159.62 182.91 126.84 131.25 173.71 183.36 120.17 136.22 178.71 183.12
Intensity Change Log 2 0.372924 0.966736 0.746964 0.267145 0.697785 0.611495 0.318521 0.832213 0.677639

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000144 0.000929 0.002201 0.001110 0.000325 0.000231 0.001558 0.001200 0.000247 0.000330 0.001835 0.001152
Bud Neck 0.001843 0.003124 0.008868 0.026249 0.002406 0.002682 0.011830 0.021454 0.002165 0.002744 0.010554 0.024008
Bud Site 0.000714 0.001227 0.001457 0.019910 0.000517 0.000862 0.001467 0.006569 0.000602 0.000914 0.001463 0.013673
Cell Periphery 0.000122 0.000119 0.000126 0.000216 0.000117 0.000045 0.000134 0.000187 0.000119 0.000055 0.000131 0.000203
Cytoplasm 0.127241* 0.066061 0.004495 0.071014 0.069285 0.026298 0.013349 0.046228 0.094126 0.031926 0.009534 0.059427
Cytoplasmic Foci 0.006906 0.007630 0.000238 0.005629 0.001749 0.002419 0.000244 0.005320 0.003959 0.003156 0.000241 0.005485
Eisosomes 0.000019 0.000025 0.000063 0.000026 0.000012 0.000011 0.000044 0.000022 0.000015 0.000013 0.000052 0.000024
Endoplasmic Reticulum 0.010286 0.036000 0.026522 0.007958 0.016509 0.020807 0.031488 0.012705 0.013842 0.022957 0.029349 0.010177
Endosome 0.001730 0.003062 0.000983 0.014333 0.001102 0.001923 0.001347 0.008269 0.001371 0.002084 0.001190 0.011498
Golgi 0.000442 0.000890 0.000131 0.005175 0.000119 0.000369 0.000110 0.006971 0.000257 0.000443 0.000119 0.006015
Lipid Particles 0.003122 0.003618 0.000957 0.003286 0.000795 0.001724 0.000683 0.001131 0.001792 0.001993 0.000801 0.002279
Mitochondria 0.004235 0.005629 0.001981 0.005529 0.002001 0.005320 0.002478 0.007858 0.002958 0.005364 0.002264 0.006618
Mitotic Spindle 0.001072 0.002329 0.000462 0.127324* 0.003003 0.004718 0.002733 0.073303 0.002175 0.004380 0.001755 0.102069*
None 0.020471 0.007243 0.000565 0.017306 0.016694 0.001994 0.003459 0.005635 0.018313 0.002737 0.002212 0.011850
Nuclear Periphery 0.047316 0.082564 0.004486 0.010366 0.074894 0.166536* 0.018673 0.012897 0.063074 0.154651* 0.012561 0.011549
Nuclear Periphery Foci 0.019439 0.051491 0.001432 0.007291 0.023508 0.017278 0.004103 0.007459 0.021764 0.022120 0.002952 0.007370
Nucleolus 0.012730 0.026299 0.011996 0.007575 0.021540 0.014358 0.009471 0.005062 0.017763 0.016048 0.010559 0.006400
Nucleus 0.738475* 0.683778* 0.909363* 0.631913* 0.762581* 0.723511* 0.880734* 0.750555* 0.752249* 0.717888* 0.893069* 0.687377*
Peroxisomes 0.000497 0.000959 0.000611 0.001135 0.000232 0.000466 0.000468 0.000313 0.000346 0.000536 0.000529 0.000751
Vacuole 0.002834 0.012787 0.022737 0.031696 0.002228 0.007505 0.015270 0.022676 0.002488 0.008252 0.018487 0.027479
Vacuole Periphery 0.000362 0.004234 0.000327 0.004958 0.000384 0.000942 0.000356 0.004187 0.000375 0.001408 0.000343 0.004598

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.15 -13.29 -16.76 -1.11 7.06 2.41 -11.54 -15.54 -16.87 3.82 -2.99 -17.36 -22.20 -17.10 7.70
Bud Neck -2.72 -7.66 -17.29 -15.65 -9.67 -1.40 -7.00 -14.82 -14.57 -3.88 -2.90 -9.46 -22.75 -22.06 -8.48
Bud Site -1.49 -5.47 -8.35 -6.35 -6.75 -1.85 -4.12 -10.99 -9.20 -3.95 -1.98 -5.69 -12.18 -10.72 -7.39
Cell Periphery -0.28 -2.38 -4.74 -2.76 -1.03 8.56 -2.92 -6.36 -15.32 -1.37 9.04 -3.77 -7.93 -18.35 -1.63
Cytoplasm 6.49 24.32 13.44 1.78 -16.49 12.46 15.43 7.43 -4.77 -9.08 18.93 27.68 14.29 -5.64 -16.93
Cytoplasmic Foci 0.03 8.89 7.06 2.16 -4.09 -1.27 4.79 0.62 1.70 -2.97 1.53 10.03 5.95 3.06 -4.78
Eisosomes -2.24 -8.97 -8.74 -2.40 6.29 0.06 -8.01 -14.38 -12.88 4.11 0.94 -11.78 -16.97 -15.15 7.01
Endoplasmic Reticulum -8.72 -8.17 1.92 9.26 8.94 -4.43 -5.82 5.93 9.64 7.92 -10.17 -9.33 6.71 14.88 11.70
Endosome -2.05 0.79 -3.04 -0.79 -3.70 -3.76 -3.59 -4.82 -2.65 -3.18 -2.99 -1.31 -5.37 -3.31 -4.78
Golgi -0.97 1.20 -1.23 -0.34 -1.94 -1.29 -2.20 -3.01 -2.47 -2.92 -0.90 0.87 -3.15 -2.51 -3.48
Lipid Particles -0.20 3.18 2.65 1.59 -0.18 -3.66 -1.07 -1.99 2.55 -0.44 -0.51 2.51 1.79 2.22 -0.45
Mitochondria -0.75 1.73 -0.23 0.76 -8.54 -4.86 -3.76 -5.04 0.26 -3.58 -3.16 0.70 -3.26 0.91 -6.50
Mitotic Spindle -1.11 -0.63 -13.93 -13.52 -13.67 -1.31 -0.74 -10.31 -9.93 -7.95 -2.16 -0.83 -17.15 -16.26 -14.50
None 6.33 11.11 4.51 -2.97 -11.59 10.62 9.13 6.63 -9.16 -5.01 13.96 14.17 7.33 -11.18 -11.13
Nuclear Periphery -3.62 11.88 10.11 7.87 -4.90 -13.34 11.75 15.74 26.18* 0.61 -15.93 15.86 18.74 27.40* -0.74
Nuclear Periphery Foci -4.14 7.25 7.49 6.42 -1.07 2.62 8.28 9.00 7.06 -0.53 0.05 10.98 11.82 9.82 -0.96
Nucleolus -3.97 1.73 -0.02 3.79 -1.64 4.52 7.42 6.47 2.45 -1.27 1.51 6.35 5.05 3.10 -2.23
Nucleus 3.02 -13.41 3.29 -0.96 15.50 4.14 -7.88 0.81 -2.75 7.76 4.21 -14.09 3.45 -0.81 15.68
Peroxisomes -1.36 -0.14 1.10 1.78 1.51 -2.96 -3.10 -1.36 2.31 2.59 -2.00 -2.43 0.37 2.23 2.69
Vacuole -4.66 -7.45 -15.00 -9.50 -4.58 -5.42 -6.47 -13.59 -11.17 -2.50 -6.60 -9.54 -20.18 -16.28 -5.02
Vacuole Periphery -2.33 -0.07 -2.30 1.86 -2.36 -3.31 0.18 -1.85 -0.57 -1.87 -3.77 0.06 -2.87 0.65 -2.91