ORF
Description Putative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.25 0.32 0.29 0.6 0.6 0.73 – 0.72 0.78 0.72 0.73 0.69 0.32 0.32 0.4 – 0.5 0.37 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.06 0 – 0 0.05 0 0 0 0 0 0
Endosome 0.09 0 0.16 0.19 0.15 0 – 0 0 0 0 0 0 0 0.05 – 0 0.06 0.07 0.09 0.11 0.07 0.11 0.1
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.25 0.12 0.11 0.06 0.19 – 0.28 0.29 0.39 0.38 0.38 0 0 0 – 0.09 0 0 0.05 0 0 0 0
Nucleus 0.05 0.05 0 0 0 0 – 0 0 0 0 0 0.17 0.17 0.08 – 0.07 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.76 0.58 0.7 0.34 0.4 0.25 – 0.17 0.13 0.16 0.14 0.16 0.56 0.57 0.62 – 0.45 0.61 0.82 0.77 0.75 0.8 0.75 0.74
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 – 0 0 0 1 0 0 1 2
Bud 0 1 1 0 0 4 – 11 5 12 20 11 0 0 0 – 0 0 0 1 1 3 3 6
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 2 2 0 – 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 – 2 4 2 1 5 1 1 0 – 0 0 0 0 0 0 0 0
Cytoplasm 61 110 88 149 227 340 – 558 391 462 373 457 81 81 122 – 72 87 2 14 4 6 17 14
Endoplasmic Reticulum 4 7 0 2 2 2 – 6 0 3 2 5 16 14 1 – 6 11 0 1 2 3 4 9
Endosome 23 0 47 47 55 20 – 28 7 6 6 9 4 1 14 – 2 14 19 40 36 19 81 65
Golgi 0 1 1 1 0 0 – 0 0 0 0 0 0 0 0 – 0 0 2 1 1 0 3 3
Mitochondria 6 86 35 28 23 88 – 220 148 253 192 250 0 0 2 – 13 7 8 22 11 10 14 22
Nucleus 12 17 1 2 3 1 – 4 2 0 2 9 42 42 24 – 10 14 4 4 0 5 11 6
Nuclear Periphery 1 5 4 0 0 0 – 5 2 3 0 3 0 0 0 – 1 0 5 1 19 3 30 24
Nucleolus 0 0 1 0 0 0 – 0 0 0 2 0 0 0 0 – 0 1 2 0 0 0 0 0
Peroxisomes 0 0 1 0 1 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 2 3 2 4 0 – 1 3 4 1 2 2 2 1 – 0 1 1 1 1 0 3 4
Vac/Vac Membrane 187 199 210 85 152 116 – 132 67 100 72 109 143 144 187 – 65 143 223 330 245 216 544 485
Unique Cell Count 245 344 302 249 377 467 778 503 644 510 666 254 251 303 144 236 273 427 328 271 724 656
Labelled Cell Count 295 428 392 316 467 572 967 629 845 671 860 291 288 351 169 278 273 427 328 271 724 656


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.2 4.9 5.1 5.0 4.2 – 4.2 4.1 3.8 4.1 4.1 5.8 5.8 6.0 4.5 5.2 5.9 6.3 6.4 6.6
Std Deviation (1e-4) 0.8 0.6 1.1 1.8 1.8 1.4 – 1.1 0.9 0.8 1.0 0.8 0.9 0.9 0.8 0.9 1.1 1.2 1.2 1.2 1.5
Intensity Change (Log2) – – – 0.08 0.04 -0.21 – -0.2 -0.24 -0.35 -0.27 -0.26 0.25 0.25 0.31 -0.11 0.1 0.29 0.37 0.39 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – -5.0 -2.2