ORF | |
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Description | Putative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud | 0 | 1 | 1 | 0 | 0 | 4 | – | 11 | 5 | 12 | 20 | 11 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 3 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 1 | – | 2 | 4 | 2 | 1 | 5 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 61 | 110 | 88 | 149 | 227 | 340 | – | 558 | 391 | 462 | 373 | 457 | 81 | 81 | 122 | – | 72 | 87 | 2 | 14 | 4 | 6 | 17 | 14 |
Endoplasmic Reticulum | 4 | 7 | 0 | 2 | 2 | 2 | – | 6 | 0 | 3 | 2 | 5 | 16 | 14 | 1 | – | 6 | 11 | 0 | 1 | 2 | 3 | 4 | 9 |
Endosome | 23 | 0 | 47 | 47 | 55 | 20 | – | 28 | 7 | 6 | 6 | 9 | 4 | 1 | 14 | – | 2 | 14 | 19 | 40 | 36 | 19 | 81 | 65 |
Golgi | 0 | 1 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 1 | 0 | 3 | 3 |
Mitochondria | 6 | 86 | 35 | 28 | 23 | 88 | – | 220 | 148 | 253 | 192 | 250 | 0 | 0 | 2 | – | 13 | 7 | 8 | 22 | 11 | 10 | 14 | 22 |
Nucleus | 12 | 17 | 1 | 2 | 3 | 1 | – | 4 | 2 | 0 | 2 | 9 | 42 | 42 | 24 | – | 10 | 14 | 4 | 4 | 0 | 5 | 11 | 6 |
Nuclear Periphery | 1 | 5 | 4 | 0 | 0 | 0 | – | 5 | 2 | 3 | 0 | 3 | 0 | 0 | 0 | – | 1 | 0 | 5 | 1 | 19 | 3 | 30 | 24 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 3 | 2 | 4 | 0 | – | 1 | 3 | 4 | 1 | 2 | 2 | 2 | 1 | – | 0 | 1 | 1 | 1 | 1 | 0 | 3 | 4 |
Vac/Vac Membrane | 187 | 199 | 210 | 85 | 152 | 116 | – | 132 | 67 | 100 | 72 | 109 | 143 | 144 | 187 | – | 65 | 143 | 223 | 330 | 245 | 216 | 544 | 485 |
Unique Cell Count | 245 | 344 | 302 | 249 | 377 | 467 | 778 | 503 | 644 | 510 | 666 | 254 | 251 | 303 | 144 | 236 | 273 | 427 | 328 | 271 | 724 | 656 | ||
Labelled Cell Count | 295 | 428 | 392 | 316 | 467 | 572 | 967 | 629 | 845 | 671 | 860 | 291 | 288 | 351 | 169 | 278 | 273 | 427 | 328 | 271 | 724 | 656 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.2 | 4.9 | 5.1 | 5.0 | 4.2 | – | 4.2 | 4.1 | 3.8 | 4.1 | 4.1 | 5.8 | 5.8 | 6.0 | 4.5 | 5.2 | 5.9 | 6.3 | 6.4 | 6.6 |
Std Deviation (1e-4) | 0.8 | 0.6 | 1.1 | 1.8 | 1.8 | 1.4 | – | 1.1 | 0.9 | 0.8 | 1.0 | 0.8 | 0.9 | 0.9 | 0.8 | 0.9 | 1.1 | 1.2 | 1.2 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.04 | -0.21 | – | -0.2 | -0.24 | -0.35 | -0.27 | -0.26 | 0.25 | 0.25 | 0.31 | -0.11 | 0.1 | 0.29 | 0.37 | 0.39 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | -5.0 | -2.2 |