ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud | 0 | 1 | 1 | 0 | 0 | 4 | – | 11 | 5 | 12 | 20 | 11 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 3 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 1 | – | 2 | 4 | 2 | 1 | 5 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 61 | 110 | 88 | 149 | 227 | 340 | – | 558 | 391 | 462 | 373 | 457 | 81 | 81 | 122 | – | 72 | 87 | 2 | 14 | 4 | 6 | 17 | 14 |
Endoplasmic Reticulum | 4 | 7 | 0 | 2 | 2 | 2 | – | 6 | 0 | 3 | 2 | 5 | 16 | 14 | 1 | – | 6 | 11 | 0 | 1 | 2 | 3 | 4 | 9 |
Endosome | 23 | 0 | 47 | 47 | 55 | 20 | – | 28 | 7 | 6 | 6 | 9 | 4 | 1 | 14 | – | 2 | 14 | 19 | 40 | 36 | 19 | 81 | 65 |
Golgi | 0 | 1 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 1 | 0 | 3 | 3 |
Mitochondria | 6 | 86 | 35 | 28 | 23 | 88 | – | 220 | 148 | 253 | 192 | 250 | 0 | 0 | 2 | – | 13 | 7 | 8 | 22 | 11 | 10 | 14 | 22 |
Nucleus | 12 | 17 | 1 | 2 | 3 | 1 | – | 4 | 2 | 0 | 2 | 9 | 42 | 42 | 24 | – | 10 | 14 | 4 | 4 | 0 | 5 | 11 | 6 |
Nuclear Periphery | 1 | 5 | 4 | 0 | 0 | 0 | – | 5 | 2 | 3 | 0 | 3 | 0 | 0 | 0 | – | 1 | 0 | 5 | 1 | 19 | 3 | 30 | 24 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 3 | 2 | 4 | 0 | – | 1 | 3 | 4 | 1 | 2 | 2 | 2 | 1 | – | 0 | 1 | 1 | 1 | 1 | 0 | 3 | 4 |
Vac/Vac Membrane | 187 | 199 | 210 | 85 | 152 | 116 | – | 132 | 67 | 100 | 72 | 109 | 143 | 144 | 187 | – | 65 | 143 | 223 | 330 | 245 | 216 | 544 | 485 |
Unique Cell Count | 245 | 344 | 302 | 249 | 377 | 467 | 778 | 503 | 644 | 510 | 666 | 254 | 251 | 303 | 144 | 236 | 273 | 427 | 328 | 271 | 724 | 656 | ||
Labelled Cell Count | 295 | 428 | 392 | 316 | 467 | 572 | 967 | 629 | 845 | 671 | 860 | 291 | 288 | 351 | 169 | 278 | 273 | 427 | 328 | 271 | 724 | 656 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.2 | 4.9 | 5.1 | 5.0 | 4.2 | – | 4.2 | 4.1 | 3.8 | 4.1 | 4.1 | 5.8 | 5.8 | 6.0 | 4.5 | 5.2 | 5.9 | 6.3 | 6.4 | 6.6 |
Std Deviation (1e-4) | 0.8 | 0.6 | 1.1 | 1.8 | 1.8 | 1.4 | – | 1.1 | 0.9 | 0.8 | 1.0 | 0.8 | 0.9 | 0.9 | 0.8 | 0.9 | 1.1 | 1.2 | 1.2 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.04 | -0.21 | – | -0.2 | -0.24 | -0.35 | -0.27 | -0.26 | 0.25 | 0.25 | 0.31 | -0.11 | 0.1 | 0.29 | 0.37 | 0.39 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | -5.0 | -2.2 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1189 | 0.7734 | 0.3959 | 0.1075 | 0.0202 | 0.4398 | 1.7342 | 1.8876 | 1.7859 | 1.6295 | 1.4351 | 1.8567 | 0.9314 | 1.7171 | 1.4097 | 1.2864 | 1.1574 | 1.4971 |
Actin | 0.0102 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0268 | 0.0002 | 0.0001 | 0.013 | 0.025 | 0.0025 | 0.0092 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0002 |
Bud | 0.0002 | 0.0109 | 0.0008 | 0.0075 | 0.0008 | 0.0003 | 0.0005 | 0.0013 | 0.0017 | 0.0038 | 0.0005 | 0.0004 | 0.0003 | 0.0035 | 0.001 | 0.0008 | 0.0003 | 0.0004 |
Bud Neck | 0.0014 | 0.0002 | 0.0005 | 0.0006 | 0.0005 | 0.0016 | 0.0008 | 0.0003 | 0.0008 | 0.0001 | 0.0003 | 0.001 | 0.0006 | 0.0004 | 0.0009 | 0.0006 | 0.0003 | 0.0012 |
Bud Periphery | 0.0004 | 0.0019 | 0.0007 | 0.0017 | 0.0031 | 0.0005 | 0.0008 | 0.0012 | 0.0025 | 0.0305 | 0.0007 | 0.0009 | 0.0007 | 0.0027 | 0.0013 | 0.0011 | 0.0002 | 0.0005 |
Bud Site | 0.001 | 0.0028 | 0.0022 | 0.0075 | 0.0006 | 0.0008 | 0.0031 | 0.0026 | 0.0017 | 0.0005 | 0.0007 | 0.0006 | 0.0008 | 0.0042 | 0.0065 | 0.001 | 0.0003 | 0.0004 |
Cell Periphery | 0.0005 | 0.0005 | 0.0003 | 0.0014 | 0.0003 | 0.0002 | 0.001 | 0.0006 | 0.0005 | 0.0004 | 0.0001 | 0.0006 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0002 |
Cytoplasm | 0.137 | 0.1749 | 0.165 | 0.0922 | 0.1232 | 0.2123 | 0.0951 | 0.2635 | 0.1829 | 0.0712 | 0.0687 | 0.1759 | 0.1872 | 0.2837 | 0.1905 | 0.2767 | 0.2396 | 0.2837 |
Cytoplasmic Foci | 0.0607 | 0.055 | 0.0562 | 0.0474 | 0.067 | 0.0678 | 0.0536 | 0.0674 | 0.0448 | 0.0228 | 0.0386 | 0.0516 | 0.0506 | 0.0357 | 0.0612 | 0.0645 | 0.0746 | 0.0699 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.007 | 0.0128 | 0.0059 | 0.0041 | 0.0035 | 0.0048 | 0.0099 | 0.0056 | 0.0053 | 0.0032 | 0.0032 | 0.0047 | 0.0055 | 0.0075 | 0.0078 | 0.0052 | 0.0026 | 0.0053 |
Endosome | 0.2863 | 0.2374 | 0.2599 | 0.3236 | 0.2696 | 0.2726 | 0.2318 | 0.2008 | 0.1992 | 0.1997 | 0.3531 | 0.2165 | 0.2712 | 0.161 | 0.3188 | 0.2994 | 0.3362 | 0.2418 |
Golgi | 0.0037 | 0.0026 | 0.0029 | 0.0028 | 0.0029 | 0.0034 | 0.0087 | 0.0031 | 0.0029 | 0.0022 | 0.0071 | 0.0031 | 0.0025 | 0.0016 | 0.0041 | 0.0034 | 0.0029 | 0.0022 |
Lipid Particles | 0.0093 | 0.0071 | 0.0057 | 0.0058 | 0.0045 | 0.0075 | 0.0236 | 0.0052 | 0.0084 | 0.0026 | 0.0046 | 0.0106 | 0.0086 | 0.0049 | 0.005 | 0.0049 | 0.0061 | 0.0032 |
Mitochondria | 0.0061 | 0.0028 | 0.005 | 0.0058 | 0.0046 | 0.0069 | 0.0192 | 0.005 | 0.0066 | 0.0076 | 0.0045 | 0.0034 | 0.0026 | 0.0022 | 0.0052 | 0.0054 | 0.002 | 0.0033 |
None | 0.2424 | 0.3044 | 0.2598 | 0.0901 | 0.2572 | 0.1815 | 0.2983 | 0.2615 | 0.3198 | 0.4217 | 0.3207 | 0.3187 | 0.2188 | 0.2855 | 0.1606 | 0.1508 | 0.1454 | 0.2056 |
Nuclear Periphery | 0.0081 | 0.0113 | 0.008 | 0.0053 | 0.0039 | 0.0057 | 0.029 | 0.0036 | 0.0056 | 0.0033 | 0.0081 | 0.0094 | 0.0085 | 0.0106 | 0.0135 | 0.0047 | 0.0023 | 0.0032 |
Nucleolus | 0.0015 | 0.0015 | 0.0013 | 0.0029 | 0.0014 | 0.0014 | 0.0021 | 0.0012 | 0.0023 | 0.0006 | 0.0003 | 0.0011 | 0.0012 | 0.0014 | 0.0013 | 0.0012 | 0.0002 | 0.0006 |
Nucleus | 0.0063 | 0.0055 | 0.0048 | 0.0049 | 0.0038 | 0.0073 | 0.0065 | 0.0038 | 0.0035 | 0.0021 | 0.0013 | 0.0035 | 0.0053 | 0.006 | 0.0049 | 0.0054 | 0.0032 | 0.0037 |
Peroxisomes | 0.0009 | 0.0006 | 0.0013 | 0.0007 | 0.0009 | 0.0015 | 0.0086 | 0.0011 | 0.002 | 0.0004 | 0.0015 | 0.0124 | 0.0005 | 0.0003 | 0.0011 | 0.0016 | 0.0009 | 0.0005 |
Punctate Nuclear | 0.0031 | 0.0017 | 0.0015 | 0.0013 | 0.0015 | 0.0017 | 0.0067 | 0.0013 | 0.0006 | 0.0005 | 0.0022 | 0.0047 | 0.0011 | 0.0013 | 0.0022 | 0.0016 | 0.0004 | 0.0009 |
Vacuole | 0.1827 | 0.1452 | 0.1846 | 0.2951 | 0.1974 | 0.1801 | 0.151 | 0.1567 | 0.1741 | 0.1654 | 0.1303 | 0.1521 | 0.2031 | 0.1571 | 0.1826 | 0.1442 | 0.1477 | 0.1471 |
Vacuole Periphery | 0.0309 | 0.0207 | 0.0332 | 0.0991 | 0.0531 | 0.0417 | 0.0225 | 0.014 | 0.0347 | 0.0481 | 0.0285 | 0.0263 | 0.0211 | 0.0299 | 0.0311 | 0.0268 | 0.0346 | 0.026 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.3414 | 6.6136 | 8.7636 | 10.1837 | 5.4813 | 9.5068 | 8.5976 | 8.8654 | 9.5919 | 11.3504 |
Translational Efficiency | 0.8694 | 0.5632 | 0.4165 | 0.4339 | 0.7817 | 0.5096 | 0.586 | 0.5391 | 0.6139 | 0.4778 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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