Standard name
Human Ortholog
Description Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.91 0.8 0.82 0.83 0.54 0.41 0.32 0.24 – 0.29 0.22 0.27 0.96 0.9 0.93 0.86 0.85 0.85 0.88 0.76 0.83 0.76 0.79 0.75
Cytoplasm 0.1 0.24 0.11 0.14 0.13 0.26 0.21 0.14 – 0.23 0.32 0.33 0.05 0.08 0.06 0.2 0.18 0.15 0.06 0.11 0 0 0 0
Endoplasmic Reticulum 0 0 0.07 0.05 0.38 0.4 0.46 0.58 – 0.36 0.31 0.31 0 0 0 0 0.05 0.1 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.05 0 0 0 0.09 0.19 0.12 0.12
Nucleus 0 0 0 0 0 0 0 0 – 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0.06 0.11 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 0 6 5 5 2 – 1 2 2 2 4 6 8 2 0 1 0 4 3 4 2
Bud 1 1 3 1 3 5 9 9 – 14 10 8 0 3 0 16 5 1 0 1 9 1 4 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 79 40 132 137 133 115 121 119 – 111 52 90 100 208 151 236 182 121 105 83 243 156 219 202
Cytoplasm 9 12 17 23 33 73 81 70 – 87 75 110 5 19 10 54 38 21 6 11 1 1 0 4
Endoplasmic Reticulum 2 2 12 8 92 111 177 286 – 137 74 106 1 5 6 12 11 14 2 3 4 1 8 13
Endosome 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0
Mitochondria 0 0 0 0 1 0 0 1 – 3 1 2 0 0 0 19 10 2 3 4 25 38 32 32
Nucleus 0 0 0 0 0 1 8 8 – 21 18 15 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 2 21 – 20 5 14 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 1 6 4 – 22 26 37 0 1 0 2 0 1 0 1 0 1 3 5
Unique Cell Count 87 50 160 165 245 281 381 490 381 238 337 104 230 163 275 213 143 120 111 292 205 278 269
Labelled Cell Count 91 55 167 170 270 312 410 520 416 264 384 108 240 173 348 249 163 120 111 292 205 278 269


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 90.3 124.1 101.1 109.7 107.6 101.6 110.6 121.9 – 122.6 115.8 118.5 117.0 101.8 89.2 59.0 81.5 95.2 113.7 105.1 110.5
Std Deviation (1e-4) 19.0 37.9 29.2 32.2 31.2 32.5 33.5 33.5 – 36.7 38.0 40.2 23.5 24.8 23.0 18.6 30.4 35.2 24.2 26.8 26.8
Intensity Change (Log2) – – – 0.12 0.09 0.01 0.13 0.27 – 0.28 0.2 0.23 0.21 0.01 -0.18 -0.78 -0.31 -0.09 0.17 0.06 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 1.1 1.3 0 0 0 0 1.9 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -5.8 -8.5 -10.8 -13.1 – -11.4 -11.9 -11.7 3.3 2.3 2.8 0.9 0.8 0.5
Cytoplasm 0.9 0.9 3.8 2.9 1.2 – 3.3 4.8 5.3 -1.7 -0.8 -1.5 2.5 1.9 1.1
Endoplasmic Reticulum -1.0 6.8 7.2 8.7 11.2 – 6.8 5.6 5.9 -2.4 -2.5 -1.5 -1.4 -0.9 0.7
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 3.4 0 0
Nucleus 0 0 0 0 0 – 3.0 3.6 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.7 – 3.0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 3.1 4.3 4.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 133.7609 202.3022 177.4005 155.7661 79.2268 168.3168 206.2454 241.3893 224.8176 211.6958 191.2078 246.4575 185.7793 232.1018 203.7144 200.342 167.3864 224.1216
Actin 0.0092 0 0.0014 0.0255 0.0021 0 0.0001 0 0.0012 0 0 0 0.0083 0 0.0001 0.0002 0.0001 0.0001
Bud 0.0003 0 0.0002 0.0001 0.0024 0.0001 0.0001 0 0.0001 0.0001 0.0013 0 0.0002 0 0.0007 0 0.0001 0.0002
Bud Neck 0.0006 0.0002 0.0009 0.0004 0.0039 0.0004 0.0003 0.0003 0.0008 0.0018 0.0022 0.0004 0.0005 0.0005 0.0012 0.001 0.0016 0.0027
Bud Periphery 0.0011 0.0001 0.0006 0.0003 0.0063 0.0013 0.0002 0.0001 0.0006 0.0007 0.0058 0.0004 0.0007 0.0002 0.0033 0.0004 0.001 0.0013
Bud Site 0.0014 0.0013 0.001 0.0004 0.0192 0.0001 0.0013 0.0019 0.0028 0.0002 0.0011 0.0001 0.0017 0.0054 0.0025 0.0004 0.0006 0.0003
Cell Periphery 0.678 0.8277 0.8218 0.7025 0.5655 0.7437 0.8067 0.8591 0.8421 0.8234 0.7603 0.86 0.7877 0.8668 0.8365 0.8376 0.7485 0.8249
Cytoplasm 0.0035 0.0006 0.0011 0.0012 0.0052 0.0001 0.0061 0.0201 0.0081 0.0081 0.0013 0.0004 0.0126 0.0098 0.0053 0.001 0.0016 0.0004
Cytoplasmic Foci 0.0188 0 0.0049 0.0168 0.0043 0 0.0011 0 0 0 0.0001 0 0.0161 0 0.0122 0.0199 0.0537 0
Eisosomes 0.0127 0.0047 0.0067 0.0075 0.0733 0.0023 0.0177 0.0064 0.0105 0.0025 0.028 0.0006 0.0194 0.0105 0.0122 0.0125 0.0061 0.0102
Endoplasmic Reticulum 0.0019 0.0008 0.0006 0.0014 0.0033 0.0007 0.0013 0.0028 0.0032 0.0077 0.0072 0.0023 0.0043 0.0015 0.0014 0.0026 0.0024 0.0012
Endosome 0.0117 0 0.0004 0.0055 0.0007 0 0 0 0.0001 0 0 0 0.0054 0 0.0001 0.0004 0.0096 0
Golgi 0.0032 0 0.0005 0.0028 0.0003 0 0 0 0.0001 0 0 0 0.0037 0 0 0.0003 0.0031 0
Lipid Particles 0.0101 0.0005 0.0047 0.0107 0.0066 0.0007 0.0016 0.0008 0.0008 0.0009 0.0008 0.0003 0.0051 0.0013 0.0017 0.0116 0.0503 0.0009
Mitochondria 0.0103 0 0.0033 0.0082 0.0501 0.0013 0.0041 0 0.0026 0 0.0027 0 0.0129 0.0001 0.0088 0.0016 0.0009 0.0002
None 0.0173 0 0.0054 0.0127 0.0423 0 0.005 0 0.0009 0 0.0034 0 0.0053 0.0001 0.0087 0.0035 0.0002 0.0001
Nuclear Periphery 0.0115 0.0098 0.0096 0.013 0.0053 0.0045 0.0141 0.0155 0.0159 0.0352 0.0211 0.0118 0.0152 0.0153 0.0119 0.0262 0.0324 0.0097
Nucleolus 0.0198 0.0006 0.0127 0.026 0.074 0.0022 0.0138 0.0005 0.003 0.0009 0.0034 0.0005 0.0046 0.0009 0.0041 0.0016 0.0017 0.0015
Nucleus 0.0052 0.0006 0.0014 0.0019 0.0088 0.001 0.0026 0.0006 0.0013 0.0016 0.0014 0.0006 0.0026 0.0009 0.0015 0.0012 0.0015 0.0013
Peroxisomes 0.0046 0 0.0056 0.0076 0.0055 0.0001 0.0003 0 0.0001 0 0.0002 0 0.003 0 0.0005 0.0013 0.0236 0.0001
Punctate Nuclear 0.0083 0 0.001 0.0076 0.0005 0 0.0002 0 0 0 0 0 0.0017 0 0.0002 0.0028 0.0015 0
Vacuole 0.1684 0.1526 0.1154 0.1463 0.1156 0.2354 0.1229 0.0917 0.1052 0.1162 0.1583 0.122 0.0874 0.0856 0.086 0.0729 0.0584 0.1423
Vacuole Periphery 0.0022 0.0003 0.0008 0.0016 0.0045 0.006 0.0006 0.0002 0.0006 0.0006 0.0013 0.0006 0.0015 0.001 0.0011 0.001 0.0011 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 265.8143 277.2736 414.0752 395.652 321.5519 280.2287 289.2766 313.6516 356.1091 343.4372
Translational Efficiency 1.1975 1.0754 0.9932 0.9834 1.015 1.1833 1.183 0.9068 1.0058 1.0

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
87 1009 1971 1304 386 1418 2696 1216 473 2427 4667 2520

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 5033.96 7567.60 8778.09 9023.37 6857.71 6519.03 7869.69 8320.93 6522.26 6954.96 8253.33 8684.41
Standard Deviation 1570.60 1690.39 1536.03 2726.06 1465.41 1423.45 1663.38 2192.89 1644.81 1624.45 1672.15 2507.80
Intensity Change Log 2 — 0.588142 0.802213 0.841973 — -0.073070 0.198580 0.279018 — 0.244375 0.485378 0.544510

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000484 0.000386 0.000562 0.002566 0.000173 0.000714 0.000308 0.000748 0.000230 0.000578 0.000416 0.001689
Bud Neck 0.005553 0.000766 0.000529 0.002208 0.001220 0.000695 0.000667 0.000937 0.002017 0.000725 0.000609 0.001595
Bud Site 0.003180 0.005089 0.003529 0.022925 0.003256 0.006479 0.006891 0.010509 0.003242 0.005901 0.005471 0.016934
Cell Periphery 0.518024* 0.940626* 0.970596* 0.720785* 0.701719* 0.956993* 0.905915* 0.904218* 0.667932* 0.950189* 0.933231* 0.809299*
Cytoplasm 0.003631 0.008547 0.000269 0.089578 0.011858 0.002630 0.057085 0.026987 0.010345 0.005090 0.033090 0.059375
Cytoplasmic Foci 0.004654 0.000256 0.000275 0.002201 0.000165 0.000608 0.000120 0.001113 0.000991 0.000461 0.000185 0.001676
Eisosomes 0.001236 0.003167 0.004293 0.001220 0.000719 0.003232 0.000507 0.001421 0.000814 0.003205 0.002106 0.001317
Endoplasmic Reticulum 0.007243 0.000648 0.000172 0.000567 0.000323 0.000559 0.000189 0.000156 0.001596 0.000596 0.000182 0.000368
Endosome 0.007198 0.000169 0.000243 0.000446 0.000096 0.000370 0.000072 0.000270 0.001402 0.000286 0.000144 0.000361
Golgi 0.010619 0.000932 0.000501 0.000828 0.000747 0.001555 0.000238 0.001358 0.002563 0.001296 0.000349 0.001084
Lipid Particles 0.029671 0.001028 0.000046 0.035024 0.002609 0.000554 0.000336 0.005546 0.007587 0.000751 0.000214 0.020800
Mitochondria 0.001167 0.000290 0.000561 0.001687 0.000075 0.000411 0.000251 0.000481 0.000276 0.000361 0.000382 0.001105
Mitotic Spindle 0.001502 0.000354 0.000311 0.000978 0.000385 0.000744 0.000118 0.000283 0.000591 0.000582 0.000199 0.000643
None 0.000016 0.000577 0.000040 0.000716 0.000047 0.000042 0.000334 0.000051 0.000041 0.000264 0.000210 0.000395
Nuclear Periphery 0.002812 0.000609 0.000964 0.002363 0.001869 0.001032 0.000450 0.001107 0.002042 0.000856 0.000667 0.001757
Nuclear Periphery Foci 0.001734 0.000480 0.000036 0.000991 0.000400 0.000342 0.000118 0.000235 0.000645 0.000399 0.000083 0.000626
Nucleolus 0.000039 0.000113 0.000096 0.000128 0.000088 0.000106 0.000072 0.000076 0.000079 0.000109 0.000082 0.000103
Nucleus 0.000359 0.000667 0.000149 0.002215 0.000939 0.000343 0.000494 0.000568 0.000832 0.000478 0.000348 0.001420
Peroxisomes 0.000089 0.000015 0.000022 0.000208 0.000032 0.000015 0.000042 0.000048 0.000043 0.000015 0.000034 0.000131
Vacuole 0.389684* 0.030017 0.013234 0.107787 0.267733* 0.018271 0.022583 0.040825 0.290164* 0.023154 0.018635 0.075475
Vacuole Periphery 0.011106 0.005262 0.003571 0.004578 0.005548 0.004303 0.003210 0.003066 0.006570 0.004702 0.003362 0.003848

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.39 -0.26 -2.91 -3.13 -2.91 -3.08 -1.95 -3.33 0.05 -2.45 -3.06 -2.41 -3.93 -2.82 -3.45
Bud Neck 2.79 2.98 1.94 -3.47 -4.76 0.84 1.02 0.39 -0.81 -1.21 2.33 2.71 0.64 -3.54 -4.64
Bud Site -1.21 -0.12 -7.62 -6.84 -7.81 -2.33 -3.44 -3.61 -1.71 -1.93 -2.59 -2.62 -8.33 -6.30 -7.28
Cell Periphery -11.34 -12.21 -5.37 23.29* 29.01* -20.03* -15.73 -14.72 8.01 0.43 -21.85 -20.63 -10.03 23.30 20.76
Cytoplasm -2.06 2.15 -13.95 -12.98 -15.01 2.90 -9.68 -3.19 -6.58 5.96 1.99 -6.87 -11.12 -14.61 -6.45
Cytoplasmic Foci 1.41 1.41 0.46 -2.80 -2.74 -1.63 0.64 -1.94 -0.91 -2.07 0.82 1.38 -1.49 -2.80 -3.41
Eisosomes -2.63 -4.07 0.05 3.55 5.37 -5.87 1.31 -2.30 3.70 -3.18 -6.87 -4.74 -2.17 5.23 2.73
Endoplasmic Reticulum 2.08 2.25 2.11 0.11 -4.58 -1.50 1.85 2.73 2.66 0.70 1.58 2.40 2.04 1.07 -3.66
Endosome 1.83 1.82 1.75 -1.93 -1.16 -2.23 1.50 -1.51 0.59 -1.71 1.53 1.76 1.41 -0.69 -2.13
Golgi 1.60 1.68 1.62 0.42 -4.78 -1.86 3.76 -0.83 0.26 -1.60 1.08 1.99 1.26 0.52 -2.18
Lipid Particles 3.30 3.42 -0.31 -9.68 -10.07 2.22 2.58 -1.90 -3.76 -4.00 3.71 4.04 -4.71 -10.31 -10.69
Mitochondria 0.82 0.55 -0.54 -2.18 -1.74 -4.16 -1.71 -2.82 -0.38 -1.27 -0.55 -0.56 -2.20 -2.16 -2.08
Mitotic Spindle 1.33 1.39 0.36 -2.30 -2.45 -1.45 9.58 3.19 1.84 -11.08 -0.07 2.49 -0.62 -0.56 -3.02
None -1.08 -0.99 -2.24 -0.33 -2.16 0.35 -0.94 -0.09 -0.78 0.94 -1.01 -0.94 -2.15 -0.59 -0.94
Nuclear Periphery 3.14 2.63 0.61 -8.12 -6.48 6.06 10.78 4.49 -0.59 -5.68 6.99 8.21 1.41 -7.05 -8.71
Nuclear Periphery Foci 2.85 5.40 2.09 -1.38 -5.05 0.25 3.94 2.27 0.53 -2.89 1.28 6.70 0.18 -1.12 -5.41
Nucleolus -6.70 -5.74 -3.22 -0.91 -1.56 -1.24 1.31 0.67 2.13 -0.56 -2.47 -0.11 -1.61 -0.05 -1.79
Nucleus -1.05 1.88 -5.57 -4.59 -6.89 1.27 1.02 0.80 -1.91 -0.96 0.97 1.34 -1.43 -5.27 -6.61
Peroxisomes 1.79 1.57 -1.67 -3.94 -3.67 2.88 -1.43 -1.35 -2.85 -0.54 2.96 0.95 -3.16 -4.43 -3.66
Vacuole 11.07 11.61 8.67 -15.75 -21.43 21.01 20.63 18.71 -7.71 -6.18 22.94 23.40 18.04 -17.84 -20.38
Vacuole Periphery 0.85 1.11 0.96 1.27 -2.00 2.29 4.93 5.23 3.94 0.82 1.43 2.52 2.11 2.66 -1.75
Standard name
Human Ortholog
Description Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (21%), cytoplasm (11%), mixed (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Hxt3

Hxt3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hxt3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available