Standard name
Human Ortholog
Description Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.1 0.16 0.15 0.18 0.2 0.22 0.22 0.26 0.21 0 0 0 0.09 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.05 0.1 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Golgi 0 0 0 0.1 0.09 0.06 0.05 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0
Mitochondria 0.13 0 0 0 0.05 0.12 0.17 0.08 0.25 0.15 0.22 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.07 0.12 0 0 0.07 0.07 0 0.05 0 0 0 0 0.07 0 0 0 0 0.06 0 0.06 0 0.07 0.06 0
Nuclear Periphery 0.19 0.09 0.08 0.2 0.07 0.12 0.13 0.13 0.1 0.07 0.08 0.05 0.07 0.15 0.14 0.11 0.2 0.14 0.08 0 0.05 0 0 0
Nucleolus 0.13 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.73 0.83 0.85 0.78 0.72 0.61 0.58 0.54 0.57 0.54 0.52 0.61 0.87 0.86 0.88 0.85 0.82 0.82 0.81 0.8 0.84 0.82 0.81 0.79
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 4 2 2 3 3 0 0 1 0 0 1 0 2 0 0 0 0
Bud 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 0 0 1 0 2 0 6 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 1 6 2 5 6 13 14 6 0 0 1 3 0 2 0 0 0 0 0 0
Cytoplasm 3 2 9 19 39 36 59 53 55 67 66 56 12 18 16 22 21 17 0 0 1 0 0 1
Endoplasmic Reticulum 0 2 2 2 4 11 18 26 9 16 7 4 1 2 7 8 6 7 0 0 0 0 0 0
Endosome 2 1 6 2 1 1 2 1 1 1 1 1 13 19 12 3 8 2 6 3 5 6 24 25
Golgi 0 3 4 18 23 15 15 5 1 8 4 3 6 7 13 15 19 6 0 0 0 0 1 1
Mitochondria 18 1 4 8 11 29 55 20 62 44 55 45 4 3 1 4 4 7 1 2 3 3 3 6
Nucleus 10 17 8 6 16 16 13 12 6 13 9 10 23 15 13 9 10 11 5 9 4 20 22 17
Nuclear Periphery 26 12 15 37 18 29 43 33 24 21 21 13 23 62 55 28 44 29 12 5 10 5 7 16
Nucleolus 18 5 11 7 4 1 3 3 4 1 2 0 17 24 14 5 5 5 2 2 2 8 6 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 1 0 0 0 0 1 2 0 0 0 0 1 1 0 0 5
Vac/Vac Membrane 99 115 168 142 176 148 194 140 143 165 132 160 304 365 349 209 185 164 120 131 177 232 311 342
Unique Cell Count 135 138 197 181 244 244 332 260 251 303 254 264 350 426 396 245 225 200 149 164 212 282 386 433
Labelled Cell Count 176 158 228 241 293 293 404 303 316 353 314 301 403 516 484 306 302 251 149 164 212 282 386 433


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.9 15.8 12.7 12.5 13.2 11.3 10.4 9.9 9.0 9.3 9.3 9.1 14.4 13.4 13.7 16.8 16.4 15.2 13.8 13.4 14.7
Std Deviation (1e-4) 2.6 4.2 3.4 2.8 3.6 3.5 3.0 2.8 2.6 2.7 2.3 2.5 4.9 3.9 3.9 6.5 7.7 5.9 3.7 4.4 4.4
Intensity Change (Log2) – – – -0.03 0.05 -0.17 -0.3 -0.36 -0.5 -0.45 -0.46 -0.48 0.18 0.08 0.11 0.4 0.37 0.25 0.12 0.08 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 2.5 2.9 0 0 0 0 0 0 0
Cytoplasm 2.2 3.8 3.5 4.4 4.9 5.2 5.3 6.1 5.1 -0.7 -0.2 -0.3 1.8 1.9 1.6
Endoplasmic Reticulum 0 0 2.2 2.6 4.0 0 2.5 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0.4 0.8 0 0 0.3 0
Golgi 3.3 3.2 2.1 1.5 0 0 0 0 0 0 0 0.9 2.1 2.9 0
Mitochondria 1.3 1.4 3.9 5.1 2.7 6.7 4.6 6.1 5.2 0 0 0 0 0 0.9
Nucleus -0.4 1.1 1.1 -0.1 0.3 -1.0 0.1 -0.3 -0.1 1.2 -0.3 -0.5 -0.2 0.2 0.7
Nuclear Periphery 3.6 -0.1 1.5 1.9 1.8 0.7 -0.3 0.3 -1.2 -0.5 2.4 2.2 1.3 3.5 2.2
Nucleolus -0.8 -2.3 -3.3 -3.2 -2.7 -2.3 0 -3.0 0 -0.4 0 -1.2 -2.0 -1.8 -1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.7 -3.3 -5.7 -6.4 -7.1 -6.5 -7.1 -7.4 -5.8 0.5 0.1 1.0 0 -0.8 -0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3393 7.9763 7.05 6.1956 5.9298 6.6255 5.8297 8.2174 7.9231 6.5621 6.5677 7.5653 6.2979 10.1098 7.9176 7.0919 7.5706 7.9151
Actin 0.0119 0.0001 0.006 0 0.0002 0.0023 0.0381 0.0078 0.0046 0.0001 0.0056 0.0002 0 0 0 0 0 0
Bud 0.0002 0.0002 0.0003 0.0017 0.0003 0.0001 0.0002 0.0002 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0 0.0002 0.0001
Bud Neck 0.0006 0.0001 0.0003 0.0002 0.0006 0.0001 0.0007 0.0003 0.0007 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0
Bud Periphery 0.0004 0.0005 0.0006 0.0016 0.0008 0.0003 0.0005 0.0003 0.0013 0.0002 0.0003 0.0004 0.0004 0.0002 0.001 0.0001 0.0008 0.001
Bud Site 0.0008 0.0004 0.0006 0.0007 0.0005 0.0001 0.0009 0.0018 0.0009 0.0002 0.0003 0.0002 0.0002 0.0001 0 0 0.0001 0
Cell Periphery 0.0013 0.0011 0.0011 0.0007 0.0004 0.0007 0.0012 0.001 0.0011 0.0003 0.0003 0.0003 0.0012 0.0011 0.0007 0.0001 0.0005 0.0011
Cytoplasm 0.0016 0.0022 0.0059 0.0011 0.0006 0.0002 0.0012 0.0024 0.0029 0.0003 0.0004 0.0002 0.0003 0.0001 0.0003 0 0.0001 0.0001
Cytoplasmic Foci 0.0082 0.0003 0.0032 0.0002 0.001 0.0013 0.0056 0.0049 0.0077 0.0051 0.03 0.0005 0.0007 0.0001 0.0006 0 0 0.0001
Eisosomes 0.0001 0 0.0001 0 0 0 0.0005 0.0001 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0025 0.0003 0.0008 0.0002 0.0006 0.0004 0.0018 0.0007 0.0011 0.0009 0.0006 0.0005 0.0003 0.0002 0.0003 0.0001 0.0003 0.0003
Endosome 0.0267 0.0062 0.0201 0.015 0.0141 0.0109 0.0381 0.0095 0.026 0.0386 0.0256 0.0096 0.0148 0.0043 0.0127 0.0024 0.0037 0.005
Golgi 0.0056 0.0002 0.0016 0.0001 0.0004 0.0011 0.0061 0.0006 0.0026 0.005 0.0126 0.0009 0.0009 0.0001 0.0006 0 0 0.0001
Lipid Particles 0.012 0.0011 0.0043 0.0001 0.0009 0.0051 0.0166 0.0011 0.0057 0.0247 0.0536 0.0072 0.002 0.0006 0.0054 0.0002 0.0002 0.0014
Mitochondria 0.0125 0.0103 0.0234 0.0003 0.0014 0.0038 0.0479 0.0088 0.0136 0.0062 0.0035 0.0202 0.0116 0.0011 0.0067 0.0002 0.0004 0.0015
None 0.001 0 0.0017 0 0.0002 0.0001 0.0013 0.0004 0.0014 0.0002 0.0003 0.0002 0.0001 0 0 0 0 0
Nuclear Periphery 0.0139 0.0161 0.01 0.0024 0.0206 0.0318 0.0105 0.0113 0.0065 0.0087 0.0037 0.028 0.0041 0.0198 0.006 0.0066 0.0068 0.0181
Nucleolus 0.0058 0.0013 0.0074 0.0007 0.007 0.001 0.0029 0.0014 0.0029 0.0009 0.0004 0.0042 0.004 0.0012 0.0024 0 0.0002 0.0014
Nucleus 0.0056 0.0022 0.0132 0.0015 0.0394 0.0016 0.0026 0.0016 0.0024 0.0011 0.0007 0.0039 0.0034 0.0011 0.0011 0.0002 0.0007 0.0013
Peroxisomes 0.0051 0.0007 0.001 0 0.0001 0.0093 0.0146 0.003 0.0099 0.0013 0.0433 0.0005 0.0003 0 0.0001 0 0 0
Punctate Nuclear 0.001 0 0.0043 0 0.0006 0.0024 0.005 0.0002 0.0046 0.0006 0.0104 0.0017 0.0001 0 0 0 0 0
Vacuole 0.3469 0.3384 0.3152 0.3056 0.2631 0.2119 0.2882 0.3049 0.2826 0.2348 0.2863 0.1527 0.2927 0.2552 0.2643 0.1645 0.2604 0.1981
Vacuole Periphery 0.5364 0.6182 0.579 0.668 0.6472 0.7156 0.5154 0.6378 0.6211 0.6707 0.5217 0.7685 0.6627 0.7145 0.6975 0.8253 0.7256 0.7702

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.4935 23.7806 16.1117 22.5487 25.4931 33.5231 23.2604 19.7563 23.9401 31.3604
Translational Efficiency 1.3769 1.278 1.378 1.0729 1.2478 0.832 1.2236 1.1359 0.8881 0.9887

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘