Standard name | |
---|---|
Human Ortholog | |
Description | Inner plaque spindle pole body (SPB) component; ortholog of human kendrin; gamma-tubulin small complex (gamma-TuSC) receptor that interacts with Spc98p to recruit the complex to the nuclear side of the SPB, connecting nuclear microtubules to the SPB; promotes gamma-TuSC assembly and oligomerization to initiate microtubule nucleation; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 1 | 0 | 1 | 0 | 2 | 3 | 3 | 8 | 11 | 4 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 2 | 10 | 10 | 17 | 13 | 25 | 6 | 18 | 0 | 0 | 1 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 1 | 4 | 9 | 8 | 8 | 11 | 17 | 15 | 2 | 5 | 9 | 15 | 20 | 18 | 29 | 20 | 20 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 1 | 4 | 0 | 0 | 2 | 0 | 0 | 3 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 6 | 3 | 1 | 2 | 0 | 0 | 0 | 2 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 0 | 0 | 0 | 2 | 1 |
Mitochondria | 3 | 8 | 79 | 118 | 77 | 173 | 203 | 233 | 252 | 278 | 183 | 244 | 1 | 0 | 21 | 12 | 3 | 11 | 0 | 0 | 0 | 0 | 3 | 6 |
Nucleus | 106 | 75 | 271 | 350 | 406 | 375 | 544 | 616 | 422 | 522 | 307 | 397 | 129 | 176 | 197 | 388 | 247 | 256 | 113 | 78 | 262 | 44 | 144 | 140 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 7 | 1 | 14 | 20 | 0 | 15 | 9 | 9 | 2 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 5 | 7 | 3 | 11 | 12 | 33 | 47 | 68 | 46 | 101 | 43 | 82 | 4 | 4 | 21 | 4 | 4 | 4 | 0 | 1 | 0 | 0 | 7 | 22 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 9 | 1 | 2 | 0 | 0 | 3 | 1 | 3 | 3 | 9 | 0 | 4 | 2 | 1 | 3 | 1 | 2 | 1 | 9 | 2 | 3 | 0 | 3 | 3 |
Vac/Vac Membrane | 3 | 0 | 0 | 3 | 2 | 3 | 5 | 1 | 0 | 1 | 1 | 1 | 0 | 2 | 5 | 15 | 14 | 18 | 0 | 2 | 13 | 3 | 19 | 44 |
Unique Cell Count | 126 | 81 | 295 | 374 | 435 | 429 | 613 | 728 | 510 | 648 | 378 | 502 | 147 | 200 | 229 | 442 | 284 | 295 | 133 | 86 | 289 | 53 | 186 | 237 |
Labelled Cell Count | 133 | 93 | 360 | 493 | 515 | 608 | 839 | 979 | 760 | 966 | 559 | 768 | 154 | 206 | 268 | 467 | 297 | 321 | 133 | 86 | 289 | 53 | 186 | 237 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 5.7 | 5.0 | 4.9 | 5.1 | 4.7 | 4.4 | 4.6 | 4.1 | 4.1 | 4.3 | 4.0 | 7.8 | 7.3 | 5.9 | 8.1 | 9.1 | 9.5 | 6.9 | 7.3 | 8.1 |
Std Deviation (1e-4) | 2.2 | 1.1 | 1.0 | 0.9 | 1.0 | 1.2 | 0.9 | 1.1 | 1.2 | 0.8 | 1.1 | 1.0 | 2.1 | 1.7 | 1.4 | 1.8 | 1.9 | 2.1 | 1.3 | 1.5 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.02 | -0.09 | -0.17 | -0.12 | -0.27 | -0.27 | -0.2 | -0.3 | 0.64 | 0.56 | 0.26 | 0.71 | 0.87 | 0.93 | 0.48 | 0.56 | 0.7 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 0 | 0 | 0 | 1.0 | 1.4 | 0 | 0 | 0 | 4.3 | 4.4 | 3.7 | 3.3 | 3.4 | 3.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.3 | -2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.7 | -8.0 | -5.1 | -9.7 | -8.9 | -7.8 |
Nucleus | 0.9 | 0.8 | -1.9 | -1.5 | -3.1 | -3.6 | -4.4 | -3.9 | -4.7 | -1.4 | -1.4 | -2.1 | -1.8 | -1.9 | -2.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.7 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.9 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.3608 | 3.2645 | 3.0256 | 2.8511 | 2.2207 | 3.332 | 3.4606 | 3.1746 | 3.2921 | 3.053 | 3.1104 | 2.7618 | 3.4714 | 3.0755 | 3.2717 | 3.4224 | 2.9548 | 3.0066 |
Actin | 0.0498 | 0.004 | 0.0076 | 0.0001 | 0.0004 | 0.0167 | 0.0028 | 0.0002 | 0.0172 | 0.0007 | 0.0625 | 0.0025 | 0.0002 | 0 | 0 | 0.0001 | 0.0029 | 0 |
Bud | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 |
Bud Neck | 0.0038 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.0006 | 0.0003 | 0.0003 | 0.0009 | 0.0002 | 0.0016 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0036 | 0.0006 | 0.0006 |
Bud Periphery | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 |
Bud Site | 0.0014 | 0.0004 | 0.0008 | 0.0001 | 0.0007 | 0 | 0.0027 | 0.0116 | 0.0011 | 0.0002 | 0.0013 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0018 | 0 | 0.0137 | 0.0027 | 0.0001 | 0.0001 | 0.0059 | 0.0001 | 0.0012 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Cytoplasmic Foci | 0.0311 | 0.0076 | 0.0246 | 0.0356 | 0.0497 | 0.0017 | 0.0365 | 0.0068 | 0.0384 | 0.0634 | 0.0744 | 0.0019 | 0.0145 | 0.0043 | 0.0077 | 0.0314 | 0.0344 | 0.0096 |
Eisosomes | 0.0007 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0005 | 0 | 0.0008 | 0.0018 | 0.0002 | 0.0003 | 0.0031 | 0 | 0.0005 | 0.0001 | 0.0014 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endosome | 0.0033 | 0.0001 | 0.0039 | 0.003 | 0.0031 | 0.0018 | 0.0273 | 0.0007 | 0.0055 | 0.0063 | 0.0115 | 0.0032 | 0.0017 | 0 | 0.0002 | 0.0005 | 0.005 | 0.0002 |
Golgi | 0.0045 | 0.0003 | 0.0023 | 0.0003 | 0.0028 | 0.0018 | 0.0037 | 0.0011 | 0.003 | 0.0059 | 0.0162 | 0.0021 | 0.0004 | 0 | 0.0003 | 0.0013 | 0.0045 | 0.0002 |
Lipid Particles | 0.0264 | 0.0015 | 0.0149 | 0.0138 | 0.0869 | 0.0016 | 0.0332 | 0.0073 | 0.0191 | 0.0186 | 0.0976 | 0.0037 | 0.0057 | 0.0005 | 0.0064 | 0.0363 | 0.0302 | 0.0029 |
Mitochondria | 0.0029 | 0.0075 | 0.0105 | 0.0004 | 0.0079 | 0.0004 | 0.004 | 0.0001 | 0.0013 | 0.0094 | 0.043 | 0.0019 | 0.0002 | 0 | 0.0001 | 0.0011 | 0.0266 | 0 |
None | 0.0034 | 0 | 0.0005 | 0.0022 | 0.0004 | 0.0006 | 0.0027 | 0 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0.0012 | 0 |
Nuclear Periphery | 0.0015 | 0.0006 | 0.0048 | 0.0199 | 0.001 | 0.003 | 0.0227 | 0.0003 | 0.0028 | 0.0003 | 0.0013 | 0.0029 | 0.0032 | 0.0001 | 0.0019 | 0.0003 | 0.002 | 0.0006 |
Nucleolus | 0.0129 | 0.055 | 0.0349 | 0.0272 | 0.0107 | 0.0155 | 0.0224 | 0.0375 | 0.0061 | 0.0031 | 0.0497 | 0.0051 | 0.0391 | 0.0041 | 0.0119 | 0.006 | 0.0623 | 0.0279 |
Nucleus | 0.1335 | 0.1337 | 0.1835 | 0.1227 | 0.0013 | 0.2326 | 0.0631 | 0.0643 | 0.0313 | 0.0011 | 0.0019 | 0.0179 | 0.3478 | 0.3784 | 0.2857 | 0.2879 | 0.2053 | 0.2623 |
Peroxisomes | 0.0221 | 0.0013 | 0.0023 | 0.0003 | 0.0073 | 0.0006 | 0.0084 | 0.0022 | 0.0088 | 0.0102 | 0.0084 | 0.0005 | 0.0005 | 0 | 0.0003 | 0.0084 | 0.0161 | 0.0002 |
Punctate Nuclear | 0.6992 | 0.7867 | 0.6927 | 0.7632 | 0.8247 | 0.7218 | 0.7555 | 0.8672 | 0.8614 | 0.8796 | 0.6247 | 0.9568 | 0.5833 | 0.6125 | 0.6848 | 0.623 | 0.6059 | 0.6953 |
Vacuole | 0.0002 | 0.0001 | 0.0002 | 0.0036 | 0.0013 | 0.0001 | 0.003 | 0.0001 | 0.0004 | 0.0003 | 0.0006 | 0.0003 | 0.0004 | 0 | 0.0001 | 0 | 0.0005 | 0 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0028 | 0.0008 | 0.0002 | 0.0021 | 0 | 0.0002 | 0.0002 | 0.0007 | 0.0005 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.5471 | 11.1337 | 4.9054 | 7.3605 | 27.2498 | 14.7817 | 15.3029 | 13.7712 | 9.3574 | 21.2923 |
Translational Efficiency | 2.1312 | 3.2657 | 1.5382 | 1.4943 | 1.3242 | 2.6563 | 1.5299 | 1.1217 | 1.3361 | 1.4776 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
24 | 948 | 521 | 350 | 1685 | 1843 | 1017 | 156 | 1709 | 2791 | 1538 | 506 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 709.81 | 790.21 | 1005.49 | 866.26 | 671.66 | 763.83 | 946.14 | 938.33 | 672.20 | 772.79 | 966.24 | 888.48 |
Standard Deviation | 94.83 | 104.59 | 124.88 | 121.96 | 101.94 | 99.32 | 118.94 | 142.40 | 101.94 | 101.91 | 124.20 | 132.85 |
Intensity Change Log 2 | — | 0.154803 | 0.502394 | 0.287367 | — | 0.185520 | 0.494323 | 0.482364 | — | 0.169819 | 0.498475 | 0.385467 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000405 | 0.000562 | 0.000561 | 0.000422 | 0.000264 | 0.000468 | 0.000154 | 0.000202 | 0.000266 | 0.000500 | 0.000292 | 0.000354 |
Bud Neck | 0.003520 | 0.004178 | 0.002378 | 0.010595 | 0.004357 | 0.010194 | 0.001421 | 0.001022 | 0.004345 | 0.008151 | 0.001745 | 0.007643 |
Bud Site | 0.008813 | 0.005474 | 0.007794 | 0.014220 | 0.006108 | 0.010321 | 0.002406 | 0.004651 | 0.006146 | 0.008675 | 0.004231 | 0.011270 |
Cell Periphery | 0.000394 | 0.000151 | 0.000064 | 0.000155 | 0.000276 | 0.000244 | 0.000039 | 0.000027 | 0.000278 | 0.000212 | 0.000047 | 0.000116 |
Cytoplasm | 0.197228* | 0.007516 | 0.023501 | 0.055850 | 0.024689 | 0.023019 | 0.004991 | 0.001006 | 0.027112 | 0.017753 | 0.011261 | 0.038941 |
Cytoplasmic Foci | 0.452159* | 0.075424 | 0.029807 | 0.041546 | 0.178983 | 0.120171 | 0.004537 | 0.030025 | 0.182819* | 0.104972 | 0.013097 | 0.037994 |
Eisosomes | 0.000093 | 0.000130 | 0.000022 | 0.000091 | 0.000207 | 0.000135 | 0.000021 | 0.000021 | 0.000205 | 0.000133 | 0.000021 | 0.000069 |
Endoplasmic Reticulum | 0.000348 | 0.001417 | 0.000780 | 0.001375 | 0.001284 | 0.001673 | 0.000531 | 0.000234 | 0.001271 | 0.001586 | 0.000615 | 0.001023 |
Endosome | 0.008312 | 0.011846 | 0.004409 | 0.015893 | 0.016915 | 0.022544 | 0.001284 | 0.003808 | 0.016794 | 0.018910 | 0.002342 | 0.012167 |
Golgi | 0.007273 | 0.011281 | 0.001429 | 0.006159 | 0.019155 | 0.014192 | 0.000648 | 0.001006 | 0.018988 | 0.013204 | 0.000912 | 0.004570 |
Lipid Particles | 0.003434 | 0.030380 | 0.009836 | 0.006306 | 0.047666 | 0.029380 | 0.002102 | 0.006545 | 0.047045 | 0.029719 | 0.004722 | 0.006380 |
Mitochondria | 0.000517 | 0.004171 | 0.000079 | 0.007732 | 0.004174 | 0.006643 | 0.000290 | 0.000070 | 0.004123 | 0.005804 | 0.000219 | 0.005370 |
Mitotic Spindle | 0.101810* | 0.654664* | 0.847694* | 0.646201* | 0.381931* | 0.471321* | 0.922354* | 0.922217* | 0.377997* | 0.533596* | 0.897063* | 0.731297* |
None | 0.013015 | 0.004353 | 0.000497 | 0.013189 | 0.018231 | 0.008007 | 0.001077 | 0.000067 | 0.018158 | 0.006766 | 0.000881 | 0.009143 |
Nuclear Periphery | 0.000293 | 0.000450 | 0.000398 | 0.000825 | 0.000535 | 0.001184 | 0.000321 | 0.000248 | 0.000531 | 0.000935 | 0.000347 | 0.000647 |
Nuclear Periphery Foci | 0.009157 | 0.079425 | 0.039700 | 0.008304 | 0.136476 | 0.091177 | 0.023199 | 0.013396 | 0.134688 | 0.087185 | 0.028788 | 0.009874 |
Nucleolus | 0.017229 | 0.035456 | 0.003574 | 0.030124 | 0.047880 | 0.075224 | 0.009871 | 0.000766 | 0.047450 | 0.061717 | 0.007738 | 0.021073 |
Nucleus | 0.020778 | 0.019361 | 0.010545 | 0.113769 | 0.023573 | 0.050568 | 0.018542 | 0.001385 | 0.023534 | 0.039968 | 0.015833 | 0.079121 |
Peroxisomes | 0.152192* | 0.051914 | 0.010715 | 0.014771 | 0.085297 | 0.057647 | 0.003784 | 0.006845 | 0.086236 | 0.055700 | 0.006132 | 0.012327 |
Vacuole | 0.002921 | 0.001545 | 0.006121 | 0.010277 | 0.001768 | 0.004784 | 0.002374 | 0.003869 | 0.001784 | 0.003684 | 0.003643 | 0.008301 |
Vacuole Periphery | 0.000109 | 0.000302 | 0.000095 | 0.002197 | 0.000231 | 0.001103 | 0.000055 | 0.002588 | 0.000229 | 0.000831 | 0.000069 | 0.002317 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.71 | -1.27 | -1.08 | -2.37 | 0.10 | -3.88 | -4.07 | -2.06 | -1.23 | -0.34 | -5.00 | -6.14 | -4.13 | -2.97 | -1.07 |
Bud Neck | -0.16 | -2.08 | -3.96 | -4.33 | -2.88 | -4.98 | -4.04 | -1.43 | 2.64 | 1.23 | -5.11 | -5.59 | -4.98 | -2.94 | -3.08 |
Bud Site | 1.83 | 0.14 | -0.13 | -4.64 | -0.57 | -4.55 | -2.79 | -1.91 | -1.28 | -1.32 | -4.27 | -6.04 | -5.10 | -4.13 | -2.59 |
Cell Periphery | 1.54 | 1.94 | 1.42 | -0.48 | -2.37 | 1.77 | 10.70 | 10.56 | 9.79 | 0.32 | 2.93 | 10.26 | 5.11 | 3.21 | -3.58 |
Cytoplasm | 2.45 | 2.12 | 1.54 | -5.49 | -3.09 | -1.05 | 3.49 | 6.39 | 7.89 | 2.05 | 1.22 | 1.21 | -3.70 | -4.20 | -4.16 |
Cytoplasmic Foci | 5.63 | 6.26 | 6.37 | 6.14 | 1.07 | 5.72 | 26.82 | 11.03 | 7.80 | -2.55 | 8.51 | 24.18 | 17.20 | 11.94 | -3.30 |
Eisosomes | -3.98 | 1.80 | -0.44 | 6.79 | -5.50 | 6.04 | 21.61 | 20.69 | 18.38 | 1.93 | 6.44 | 22.27 | 16.12 | 13.48 | -4.53 |
Endoplasmic Reticulum | -4.49 | -3.05 | -3.64 | -1.56 | -1.59 | -1.18 | -0.27 | 5.97 | 6.48 | 4.13 | -2.56 | -0.92 | -1.66 | -1.04 | -1.18 |
Endosome | 0.35 | 0.90 | -0.10 | -0.50 | -1.08 | -2.38 | 11.37 | 5.39 | 6.67 | -0.39 | -0.78 | 9.86 | 1.90 | 2.28 | -2.02 |
Golgi | -2.53 | 2.14 | 1.36 | 6.25 | -1.03 | 5.01 | 19.35 | 18.88 | 17.66 | 0.40 | 5.47 | 18.99 | 13.09 | 10.63 | -1.52 |
Lipid Particles | -6.07 | -0.73 | 0.63 | 7.07 | 1.58 | 7.11 | 16.87 | 10.40 | 5.73 | -1.56 | 6.93 | 15.80 | 14.14 | 10.80 | -1.22 |
Mitochondria | -0.99 | 1.34 | -2.01 | -1.30 | -2.32 | -1.79 | 1.98 | 2.47 | 5.41 | 2.04 | -1.34 | 2.17 | -1.21 | -0.54 | -2.18 |
Mitotic Spindle | -7.54 | -10.31 | -7.79 | -1.49 | 4.40 | -6.10 | -34.22* | -22.88 | -19.21 | -1.44 | -10.28 | -34.13 | -15.84 | -10.08 | 5.59 |
None | 1.45 | 1.79 | 0.66 | -1.86 | -2.73 | 4.25 | 7.41 | 8.76 | 9.33 | 1.84 | 5.02 | 8.00 | 1.73 | -0.86 | -2.48 |
Nuclear Periphery | -1.56 | -3.32 | -4.69 | -4.39 | -3.03 | -4.84 | -2.14 | -1.18 | 1.02 | -0.44 | -4.29 | -3.13 | -4.24 | -2.47 | -3.15 |
Nuclear Periphery Foci | -5.98 | -2.31 | 1.29 | 13.79 | 6.43 | 5.06 | 18.87 | 17.43 | 13.77 | 2.48 | 6.17 | 18.16 | 23.34 | 21.80 | 6.47 |
Nucleolus | -3.51 | 0.89 | -2.83 | 0.15 | -5.18 | -5.90 | 3.88 | 14.19 | 17.56 | 7.91 | -4.90 | 6.60 | 2.06 | 5.38 | -1.98 |
Nucleus | -0.40 | -1.42 | -6.77 | -7.13 | -6.28 | -5.80 | -5.10 | 1.29 | 5.60 | 5.34 | -5.26 | -5.45 | -7.07 | -5.75 | -4.75 |
Peroxisomes | 2.00 | 3.24 | 3.30 | 9.65 | 0.54 | 5.17 | 21.49 | 16.23 | 12.69 | -1.42 | 5.85 | 20.68 | 18.22 | 16.15 | -1.72 |
Vacuole | 0.95 | -4.83 | -4.09 | -4.37 | -1.82 | -3.06 | -3.62 | -1.62 | -1.30 | -0.90 | -3.16 | -6.87 | -4.72 | -4.43 | -2.95 |
Vacuole Periphery | -1.30 | -2.27 | -1.66 | -1.56 | -1.52 | -4.04 | -1.11 | -0.98 | -0.75 | -0.97 | -4.21 | -2.25 | -1.84 | -1.45 | -1.78 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|