Standard name
Description Inner plaque spindle pole body (SPB) component; ortholog of human kendrin; gamma-tubulin small complex (gamma-TuSC) receptor that interacts with Spc98p to recruit the complex to the nuclear side of the SPB, connecting nuclear microtubules to the SPB; promotes gamma-TuSC assembly and oligomerization to initiate microtubule nucleation; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0 0 0 0 0 0 0 0 0 0.10 0.10 0.08 0.07 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.10 0.27 0.32 0.18 0.40 0.33 0.32 0.49 0.43 0.48 0.49 0 0 0.09 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.93 0.92 0.94 0.93 0.87 0.89 0.85 0.83 0.81 0.81 0.79 0.88 0.88 0.86 0.88 0.87 0.87 0.85 0.92 0.91 0.85 0.78 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.09 0 0 0 0.08 0.08 0.09 0.09 0.16 0.11 0.16 0 0 0.09 0 0 0 0 0 0 0 0 0.10
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0.05 0.07 0.11 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 1 0 2 3 3 8 11 4 4 0 0 0 1 0 0 1 0 1 0 0 3
Bud Neck 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 2 10 10 17 13 25 6 18 0 0 1 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 1 4 9 8 8 11 17 15 2 5 9 15 20 18 29 20 20 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 9 1 4 0 0 2 0 0 3
Endosome 1 0 0 0 1 0 0 1 0 1 0 0 0 3 0 6 3 1 2 0 0 0 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 2 0 0 0 2 1
Mitochondria 3 8 79 118 77 173 203 233 252 278 183 244 1 0 21 12 3 11 0 0 0 0 3 6
Nucleus 106 75 271 350 406 375 544 616 422 522 307 397 129 176 197 388 247 256 113 78 262 44 144 140
Nuclear Periphery 0 0 0 0 7 1 14 20 0 15 9 9 2 0 1 1 2 1 0 0 0 0 0 0
Nucleolus 5 7 3 11 12 33 47 68 46 101 43 82 4 4 21 4 4 4 0 1 0 0 7 22
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 9 1 2 0 0 3 1 3 3 9 0 4 2 1 3 1 2 1 9 2 3 0 3 3
Vac/Vac Membrane 3 0 0 3 2 3 5 1 0 1 1 1 0 2 5 15 14 18 0 2 13 3 19 44
Unique Cell Count 126 81 295 374 435 429 613 728 510 648 378 502 147 200 229 442 284 295 133 86 289 53 186 237
Labelled Cell Count 133 93 360 493 515 608 839 979 760 966 559 768 154 206 268 467 297 321 133 86 289 53 186 237

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

SpindlePole

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 5.7 5.0 4.9 5.1 4.7 4.4 4.6 4.1 4.1 4.3 4.0 7.8 7.3 5.9 8.1 9.1 9.5 6.9 7.3 8.1
Std Deviation (1e-4) 2.2 1.1 1.0 0.9 1.0 1.2 0.9 1.1 1.2 0.8 1.1 1.0 2.1 1.7 1.4 1.8 1.9 2.1 1.3 1.5 2.4
Intensity Change (Log2) -0.0 0.0 -0.1 -0.2 -0.1 -0.3 -0.3 -0.2 -0.3 0.6 0.6 0.3 0.7 0.9 0.9 0.5 0.6 0.7

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 3.7 3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.3 -2.9 0 0 0 0 0 0 0 -6.7 -8.0 -5.1 -9.7 -8.9 -7.8
Nucleus 0 3.3 0 0 0 0 0 0 0 6.8 6.9 5.9 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.7 1.6 0 0 0 0 0 0 0 0 0 4.4 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 3.6