Standard name
Human Ortholog
Description Inner plaque spindle pole body (SPB) component; ortholog of human kendrin; gamma-tubulin small complex (gamma-TuSC) receptor that interacts with Spc98p to recruit the complex to the nuclear side of the SPB, connecting nuclear microtubules to the SPB; promotes gamma-TuSC assembly and oligomerization to initiate microtubule nucleation; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0 0 0 0 0 0 0 0 0 0.1 0.1 0.08 0.07 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0.27 0.32 0.18 0.4 0.33 0.32 0.49 0.43 0.48 0.49 0 0 0.09 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.93 0.92 0.94 0.93 0.87 0.89 0.85 0.83 0.81 0.81 0.79 0.88 0.88 0.86 0.88 0.87 0.87 0.85 0.92 0.91 0.85 0.78 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.09 0 0 0 0.08 0.08 0.09 0.09 0.16 0.11 0.16 0 0 0.09 0 0 0 0 0 0 0 0 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0.05 0.07 0.11 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 1 0 2 3 3 8 11 4 4 0 0 0 1 0 0 1 0 1 0 0 3
Bud Neck 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 2 10 10 17 13 25 6 18 0 0 1 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 1 4 9 8 8 11 17 15 2 5 9 15 20 18 29 20 20 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 9 1 4 0 0 2 0 0 3
Endosome 1 0 0 0 1 0 0 1 0 1 0 0 0 3 0 6 3 1 2 0 0 0 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 2 0 0 0 2 1
Mitochondria 3 8 79 118 77 173 203 233 252 278 183 244 1 0 21 12 3 11 0 0 0 0 3 6
Nucleus 106 75 271 350 406 375 544 616 422 522 307 397 129 176 197 388 247 256 113 78 262 44 144 140
Nuclear Periphery 0 0 0 0 7 1 14 20 0 15 9 9 2 0 1 1 2 1 0 0 0 0 0 0
Nucleolus 5 7 3 11 12 33 47 68 46 101 43 82 4 4 21 4 4 4 0 1 0 0 7 22
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 9 1 2 0 0 3 1 3 3 9 0 4 2 1 3 1 2 1 9 2 3 0 3 3
Vac/Vac Membrane 3 0 0 3 2 3 5 1 0 1 1 1 0 2 5 15 14 18 0 2 13 3 19 44
Unique Cell Count 126 81 295 374 435 429 613 728 510 648 378 502 147 200 229 442 284 295 133 86 289 53 186 237
Labelled Cell Count 133 93 360 493 515 608 839 979 760 966 559 768 154 206 268 467 297 321 133 86 289 53 186 237


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 5.7 5.0 4.9 5.1 4.7 4.4 4.6 4.1 4.1 4.3 4.0 7.8 7.3 5.9 8.1 9.1 9.5 6.9 7.3 8.1
Std Deviation (1e-4) 2.2 1.1 1.0 0.9 1.0 1.2 0.9 1.1 1.2 0.8 1.1 1.0 2.1 1.7 1.4 1.8 1.9 2.1 1.3 1.5 2.4
Intensity Change (Log2) – – – -0.03 0.02 -0.09 -0.17 -0.12 -0.27 -0.27 -0.2 -0.3 0.64 0.56 0.26 0.71 0.87 0.93 0.48 0.56 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0 0 0 1.0 1.4 0 0 0 4.3 4.4 3.7 3.3 3.4 3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.3 -2.9 0 0 0 0 0 0 0 -6.7 -8.0 -5.1 -9.7 -8.9 -7.8
Nucleus 0.9 0.8 -1.9 -1.5 -3.1 -3.6 -4.4 -3.9 -4.7 -1.4 -1.4 -2.1 -1.8 -1.9 -2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.7 1.6 0 0 0 0 0 0 0 0 0 4.4 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.2 3.9 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3608 3.2645 3.0256 2.8511 2.2207 3.332 3.4606 3.1746 3.2921 3.053 3.1104 2.7618 3.4714 3.0755 3.2717 3.4224 2.9548 3.0066
Actin 0.0498 0.004 0.0076 0.0001 0.0004 0.0167 0.0028 0.0002 0.0172 0.0007 0.0625 0.0025 0.0002 0 0 0.0001 0.0029 0
Bud 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0005 0 0 0 0 0 0.0002 0
Bud Neck 0.0038 0.0006 0.0006 0.0002 0.0004 0.0006 0.0003 0.0003 0.0009 0.0002 0.0016 0.0001 0.0007 0 0.0001 0.0036 0.0006 0.0006
Bud Periphery 0.0004 0.0001 0.0005 0.0001 0.0002 0.0001 0.0003 0.0001 0.0002 0 0.001 0.0001 0.0001 0 0 0 0.0004 0
Bud Site 0.0014 0.0004 0.0008 0.0001 0.0007 0 0.0027 0.0116 0.0011 0.0002 0.0013 0 0.0005 0 0.0001 0.0001 0.0007 0
Cell Periphery 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0 0.0002 0 0 0 0 0 0.0001 0
Cytoplasm 0.0018 0 0.0137 0.0027 0.0001 0.0001 0.0059 0.0001 0.0012 0.0002 0.0004 0.0001 0.0007 0 0.0001 0 0.0004 0
Cytoplasmic Foci 0.0311 0.0076 0.0246 0.0356 0.0497 0.0017 0.0365 0.0068 0.0384 0.0634 0.0744 0.0019 0.0145 0.0043 0.0077 0.0314 0.0344 0.0096
Eisosomes 0.0007 0.0003 0.0004 0 0.0001 0.0002 0.0001 0 0.0001 0 0.0005 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0005 0 0.0008 0.0018 0.0002 0.0003 0.0031 0 0.0005 0.0001 0.0014 0.0002 0.0003 0 0.0001 0 0.0002 0
Endosome 0.0033 0.0001 0.0039 0.003 0.0031 0.0018 0.0273 0.0007 0.0055 0.0063 0.0115 0.0032 0.0017 0 0.0002 0.0005 0.005 0.0002
Golgi 0.0045 0.0003 0.0023 0.0003 0.0028 0.0018 0.0037 0.0011 0.003 0.0059 0.0162 0.0021 0.0004 0 0.0003 0.0013 0.0045 0.0002
Lipid Particles 0.0264 0.0015 0.0149 0.0138 0.0869 0.0016 0.0332 0.0073 0.0191 0.0186 0.0976 0.0037 0.0057 0.0005 0.0064 0.0363 0.0302 0.0029
Mitochondria 0.0029 0.0075 0.0105 0.0004 0.0079 0.0004 0.004 0.0001 0.0013 0.0094 0.043 0.0019 0.0002 0 0.0001 0.0011 0.0266 0
None 0.0034 0 0.0005 0.0022 0.0004 0.0006 0.0027 0 0.0004 0.0001 0.0005 0.0001 0.0005 0 0 0 0.0012 0
Nuclear Periphery 0.0015 0.0006 0.0048 0.0199 0.001 0.003 0.0227 0.0003 0.0028 0.0003 0.0013 0.0029 0.0032 0.0001 0.0019 0.0003 0.002 0.0006
Nucleolus 0.0129 0.055 0.0349 0.0272 0.0107 0.0155 0.0224 0.0375 0.0061 0.0031 0.0497 0.0051 0.0391 0.0041 0.0119 0.006 0.0623 0.0279
Nucleus 0.1335 0.1337 0.1835 0.1227 0.0013 0.2326 0.0631 0.0643 0.0313 0.0011 0.0019 0.0179 0.3478 0.3784 0.2857 0.2879 0.2053 0.2623
Peroxisomes 0.0221 0.0013 0.0023 0.0003 0.0073 0.0006 0.0084 0.0022 0.0088 0.0102 0.0084 0.0005 0.0005 0 0.0003 0.0084 0.0161 0.0002
Punctate Nuclear 0.6992 0.7867 0.6927 0.7632 0.8247 0.7218 0.7555 0.8672 0.8614 0.8796 0.6247 0.9568 0.5833 0.6125 0.6848 0.623 0.6059 0.6953
Vacuole 0.0002 0.0001 0.0002 0.0036 0.0013 0.0001 0.003 0.0001 0.0004 0.0003 0.0006 0.0003 0.0004 0 0.0001 0 0.0005 0
Vacuole Periphery 0.0002 0.0001 0.0002 0.0028 0.0008 0.0002 0.0021 0 0.0002 0.0002 0.0007 0.0005 0.0001 0 0.0001 0 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.5471 11.1337 4.9054 7.3605 27.2498 14.7817 15.3029 13.7712 9.3574 21.2923
Translational Efficiency 2.1312 3.2657 1.5382 1.4943 1.3242 2.6563 1.5299 1.1217 1.3361 1.4776

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Inner plaque spindle pole body (SPB) component; ortholog of human kendrin; gamma-tubulin small complex (gamma-TuSC) receptor that interacts with Spc98p to recruit the complex to the nuclear side of the SPB, connecting nuclear microtubules to the SPB; promotes gamma-TuSC assembly and oligomerization to initiate microtubule nucleation; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
Localization
Cell Percentages spindle pole (94%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Spc110

Spc110


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc110-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available