Standard name
Description Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.82 0.77 0.78 0.8 0.8 0.8 0.79 0.78 0.83 0.79 0.72 0.8 0.69 0.73 0.89 0.92 0.89 0.44 0.42 0.44 0.4 0.37 0.38
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Endosome 0.06 0.16 0 0 0.09 0 0 0 0 0 0 0.18 0.2 0.2 0.07 0.06 0 0.27 0.34 0.22 0.26 0.29 0.28
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.17 0.3 0.13 0.31 0.43 0.41 0.36 0.41 0.49 0 0 0 0 0 0 0.05 0.06 0.12 0.1 0.06 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0.05 0.09 0 0.05 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0.07 0 0.07 0.06 0.09 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.1 0.09 0.06 0.12 0.13 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 14 7 3 1 0 3
Bud 9 7 17 13 8 17 21 14 27 17 17 0 1 0 0 1 0 6 1 1 1 3 4
Bud Neck 6 0 11 0 2 2 2 0 2 1 1 0 1 1 1 1 0 7 3 2 1 0 1
Bud Site 0 0 0 0 0 1 1 3 2 1 2 0 0 0 0 0 0
Cell Periphery 0 1 0 0 0 0 0 1 1 4 2 0 0 0 3 3 2 1 0 0 0 0 0
Cytoplasm 581 323 328 326 404 437 677 517 638 476 427 144 107 166 141 208 144 384 194 212 80 98 113
Endoplasmic Reticulum 4 5 2 0 1 1 0 0 0 1 0 2 2 0 8 11 11 1 0 3 1 1 2
Endosome 45 65 2 12 44 10 8 9 6 5 2 32 31 45 11 13 6 237 159 107 52 77 84
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 2 0 1 3 0 0 3 6
Mitochondria 27 6 70 122 65 172 367 268 275 246 291 0 1 1 6 4 7 39 28 59 19 16 20
Nucleus 0 0 2 0 3 3 7 1 2 3 2 0 1 0 0 0 2 1 0 1 0 0 0
Nuclear Periphery 0 2 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 1 0 0 1 0 2 0 0 1 1 0 0 0 0 1 1 0 0 0 0 1 0
Peroxisomes 0 1 0 4 6 8 1 2 4 1 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 46 22 36 12 26 16 13 14 18 7 9 8 14 12 4 5 4 57 17 34 11 22 15
Vac/Vac Membrane 25 17 3 5 8 10 5 4 6 3 7 8 8 10 4 6 3 87 39 29 23 34 41
Unique Cell Count 709 419 420 409 505 548 859 661 773 606 592 179 156 226 158 227 161 865 465 480 202 269 299
Labelled Cell Count 745 451 472 494 569 677 1105 833 981 766 761 194 168 238 180 255 180 865 465 480 202 269 299


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.6 3.3 3.2 4.0 3.2 3.3 2.9 3.2 3.4 2.9 5.1 5.3 5.0 5.6 5.5 5.3 4.6 5.1 5.3
Std Deviation (1e-4) 0.4 0.8 0.7 1.2 1.4 0.9 1.2 1.1 0.9 6.4 0.6 1.1 1.4 0.9 1.4 1.2 1.4 1.1 1.3 1.4
Intensity Change (Log2) -0.05 0.26 -0.06 0.0 -0.21 -0.07 0.04 -0.19 0.62 0.66 0.59 0.74 0.73 0.67 0.48 0.61 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cnl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available