TheCellVision

ESC2

Standard name	ESC2 ORF YDR363W
Human Ortholog
Description	Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member

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Scale images

ESC2

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0.05	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cytoplasm	0	0	0.08	0.14	0.16	0.11	0.12	0.1	0.07	0.07	0.06	0.05	0.12	0.24	0.27	0.22	0.27	0.23	0	0	0	0.05	0	0
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.06	0	0.05	0	0	0	0	0	0
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0.38	0.29	0.21	0.28	0.4	0.72	0.69	0.59	0.62	0.75	0.72	0.77	0	0	0	0	0.06	0	0	0	0	0	0	0
Nucleus	0.81	0.82	0.79	0.68	0.59	0.61	0.55	0.66	0.67	0.61	0.66	0.63	0.84	0.71	0.66	0.67	0.58	0.52	0.8	0.8	0.72	0.71	0.61	0.52
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	0.06	0.05	0	0.09	0.05	0	0	0	0	0	0	0	0.08	0.06	0.16	0.21	0.25	0.13	0.11	0.15	0.15	0.22	0.23

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	1
Bud	1	0	0	0	4	3	4	4	3	5	4	3	0	0	0	0	0	1	2	4	3	3	3	3
Bud Neck	1	0	0	0	0	0	0	0	1	0	0	0	1	1	0	0	0	0	1	1	0	0	0	1
Bud Site	0	0	0	1	2	8	3	10	9	17	19	10	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0
Cytoplasm	17	12	25	21	33	27	30	38	32	34	24	22	38	85	103	19	26	26	6	9	8	18	9	12
Endoplasmic Reticulum	1	1	0	0	0	0	0	0	0	0	0	0	1	2	1	5	3	6	0	0	1	0	0	1
Endosome	0	0	1	2	6	0	0	0	3	1	0	0	5	3	6	1	0	3	6	2	7	2	3	4
Golgi	0	0	2	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	2	0	5	0	0	3
Mitochondria	177	95	68	42	84	181	174	231	278	347	272	325	0	4	8	2	6	5	3	4	4	11	7	11
Nucleus	383	274	253	101	124	153	140	257	302	282	249	266	273	257	252	58	56	59	401	281	266	276	159	151
Nuclear Periphery	1	0	0	1	1	1	0	1	4	1	3	5	0	1	2	0	0	1	1	0	1	0	1	3
Nucleolus	0	0	0	0	3	1	0	3	3	5	5	8	0	0	0	0	0	0	1	0	0	3	5	10
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0
SpindlePole	0	1	0	3	2	0	0	2	1	1	4	0	1	0	3	0	1	0	3	5	7	8	6	9
Vac/Vac Membrane	16	20	16	5	19	12	9	12	18	17	9	16	12	29	23	14	20	29	67	37	56	58	56	67
Unique Cell Count	471	333	321	148	210	251	253	391	449	464	380	424	324	361	380	87	97	114	504	353	369	388	260	290
Labelled Cell Count	597	403	365	176	278	386	360	558	654	710	589	655	331	383	399	99	112	131	504	353	369	388	260	290

Yeast GFP Assignment

Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	3.8	4.5	4.6	4.5	4.5	3.8	3.9	3.9	3.8	3.5	3.3	3.4	6.2	6.1	5.6	7.0	6.7	6.8	6.3	6.5	6.7
Std Deviation (1e^-4)	0.5	0.7	1.2	1.9	1.8	1.7	2.0	1.9	1.4	1.1	1.3	1.3	1.2	1.5	1.2	1.4	1.5	1.5	1.6	1.8	1.7
Intensity Change (Log₂)	–	–	–	-0.03	-0.04	-0.27	-0.24	-0.22	-0.27	-0.39	-0.49	-0.45	0.45	0.42	0.29	0.61	0.55	0.56	0.47	0.51	0.55

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Actin	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Bud	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Bud Neck	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Bud Site	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cell Periphery	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cyto	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Endoplasmic Reticulum	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Endosome	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Golgi	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Mitochondria	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nuclear Periphery	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nuc	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Nucleolus	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Peroxisomes	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
SpindlePole	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Vac	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Cortical Patches	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cytoplasm	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nucleus	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Vacuole	–	–	–	–	–	–	–	–	–	–	–	–	3.5	4.8	6.2

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	4.6139	4.6274	4.6879	4.3121	4.1365	4.3461	2.4773	2.4851	2.5202	1.9407	1.905	2.3295	1.9644	2.1149	1.7613	1.8221	1.5441	2.1173
Actin	0.005	0.0002	0.0071	0.0011	0.001	0.006	0.0033	0	0.004	0.0045	0	0	0.0018	0	0	0	0	0
Bud	0.0006	0.0002	0.0002	0.0004	0.0023	0.0003	0.0001	0	0.0001	0.0001	0	0	0.0009	0	0	0	0	0
Bud Neck	0.0033	0.0004	0.0008	0.0011	0.0004	0.0041	0.0018	0.0002	0.0006	0.0008	0.0018	0.0008	0.004	0.0001	0.0001	0	0.0003	0.0004
Bud Periphery	0.0016	0.0001	0.0006	0.0026	0.0009	0.0011	0.0002	0.0001	0.0001	0.0002	0	0	0.0002	0	0	0	0	0
Bud Site	0.0074	0.0011	0.0009	0.0009	0.0004	0.0004	0.0002	0.0003	0.0003	0.0019	0.0001	0	0.0003	0.0001	0	0	0	0
Cell Periphery	0.002	0	0.0006	0.0003	0.0003	0.0002	0.0001	0	0	0	0	0	0.0001	0	0	0	0	0
Cytoplasm	0.0069	0.0102	0.013	0.007	0.033	0.0035	0.0015	0.0049	0.0003	0.0002	0.0026	0.0002	0.0029	0.0041	0.0033	0.0001	0.0003	0.0045
Cytoplasmic Foci	0.0147	0.0004	0.0014	0.0003	0.0072	0.0011	0.0019	0.0001	0.0002	0.0043	0.0001	0	0.0015	0	0.0001	0	0	0.0032
Eisosomes	0.0002	0	0.0001	0.0001	0.0001	0	0.0001	0	0	0	0	0	0	0	0	0	0	0
Endoplasmic Reticulum	0.0045	0.0005	0.0045	0.0005	0.002	0.0013	0.0016	0.0001	0.0002	0	0.0001	0	0.0003	0.0004	0.0004	0	0	0
Endosome	0.0145	0.0005	0.0025	0.0046	0.007	0.0027	0.0075	0.0002	0.0009	0.0011	0.0004	0	0.0025	0.0002	0.0003	0	0	0
Golgi	0.0035	0	0.0023	0.0002	0.0006	0.0012	0.0014	0	0.0003	0.0014	0	0	0.0004	0	0	0	0	0
Lipid Particles	0.0097	0.0001	0.0005	0.0002	0.0062	0.0014	0.0079	0	0.0002	0.0027	0	0	0.0011	0	0.0001	0	0	0
Mitochondria	0.0159	0.0003	0.0019	0.018	0.0028	0.001	0.0022	0.0001	0.0002	0.0005	0.0001	0.0001	0.0004	0.0001	0.0001	0.0001	0	0
None	0.0067	0.0116	0.0219	0.0124	0.0358	0.0006	0.0016	0.0027	0.0002	0.0002	0.0002	0	0.0029	0.0029	0.0026	0	0.0001	0.0009
Nuclear Periphery	0.0344	0.0058	0.0089	0.0076	0.0083	0.0055	0.009	0.0036	0.0039	0.001	0.0021	0.0011	0.0047	0.0068	0.0031	0.0003	0.0014	0.0013
Nucleolus	0.0079	0.0068	0.006	0.0161	0.0109	0.0132	0.0058	0.0055	0.0042	0.0032	0.0081	0.0085	0.0031	0.0029	0.0021	0.0032	0.008	0.0057
Nucleus	0.8035	0.9367	0.8742	0.8989	0.8515	0.9395	0.9407	0.9775	0.9793	0.9687	0.9798	0.9828	0.9555	0.9783	0.9861	0.9961	0.9849	0.9747
Peroxisomes	0.0077	0	0.0002	0.0001	0.0009	0.0002	0.0009	0	0.0001	0.007	0	0	0.0004	0	0	0	0	0
Punctate Nuclear	0.0414	0.0234	0.0509	0.004	0.0061	0.0151	0.0098	0.004	0.0047	0.0018	0.0042	0.0063	0.0157	0.0035	0.0015	0.0002	0.0048	0.0091
Vacuole	0.0055	0.0013	0.0013	0.0207	0.0175	0.0012	0.0013	0.0005	0.0001	0.0001	0.0004	0.0001	0.0009	0.0004	0.0002	0	0.0001	0.0001
Vacuole Periphery	0.0032	0.0002	0.0003	0.0029	0.0049	0.0003	0.0011	0.0001	0.0001	0	0	0	0.0002	0.0001	0	0	0	0

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	4.8289	7.914	9.0758	7.7367	8.3478	4.8262	8.5475	5.1552	8.7137	7.4451
Translational Efficiency	1.2664	0.8441	0.7122	0.7905	0.5579	1.1917	0.7604	1.2113	0.77	0.7952

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
1681	821	2859	1461	1474	1405	212	320	3155	2226	3071	1781

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	611.02	755.57	814.11	861.64	671.96	760.60	933.24	830.20	639.49	758.74	822.33	855.99
Standard Deviation	84.15	104.82	106.95	126.12	85.11	99.47	111.96	161.62	89.90	101.50	111.47	133.75
Intensity Change Log ₂	—	0.306346	0.414004	0.495866	—	0.178763	0.473873	0.305084	—	0.240933	0.445670	0.399094

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000060	0.000359	0.000283	0.001210	0.000125	0.001033	0.000449	0.000633	0.000090	0.000784	0.000295	0.001106
Bud Neck	0.002917	0.004767	0.014847	0.020765	0.001594	0.008312	0.001988	0.014334	0.002299	0.007005	0.013959	0.019610
Bud Site	0.002342	0.002688	0.004686	0.015571	0.000519	0.004840	0.000392	0.024330	0.001490	0.004046	0.004390	0.017145
Cell Periphery	0.000402	0.000146	0.000197	0.000220	0.000122	0.000170	0.000041	0.000913	0.000272	0.000161	0.000186	0.000345
Cytoplasm	0.142408*	0.064261	0.025662	0.028980	0.089127	0.056241	0.002952	0.152330*	0.117515*	0.059199	0.024095	0.051143
Cytoplasmic Foci	0.022677	0.010542	0.000460	0.005931	0.013137	0.010536	0.000101	0.034728	0.018220	0.010538	0.000435	0.011105
Eisosomes	0.000039	0.000029	0.000018	0.000031	0.000026	0.000034	0.000028	0.000169	0.000033	0.000032	0.000019	0.000055
Endoplasmic Reticulum	0.002317	0.005148	0.003685	0.011552	0.001371	0.004218	0.006172	0.002474	0.001875	0.004561	0.003856	0.009921
Endosome	0.000958	0.000957	0.000715	0.007148	0.000820	0.002569	0.000177	0.013564	0.000894	0.001974	0.000678	0.008301
Golgi	0.000515	0.000710	0.000136	0.006216	0.000398	0.002643	0.000029	0.020338	0.000460	0.001930	0.000129	0.008754
Lipid Particles	0.004367	0.001815	0.000186	0.001100	0.002522	0.003859	0.000254	0.007277	0.003505	0.003105	0.000191	0.002210
Mitochondria	0.003084	0.002495	0.001652	0.009046	0.002709	0.006976	0.000408	0.010018	0.002909	0.005323	0.001566	0.009221
Mitotic Spindle	0.000739	0.007335	0.003395	0.037275	0.001295	0.009748	0.000026	0.009144	0.000998	0.008858	0.003162	0.032221
None	0.024365	0.007291	0.004796	0.004266	0.007789	0.003895	0.001631	0.040881	0.016621	0.005147	0.004577	0.010845
Nuclear Periphery	0.001810	0.001408	0.001075	0.003566	0.001161	0.002439	0.000507	0.000792	0.001507	0.002059	0.001036	0.003068
Nuclear Periphery Foci	0.000682	0.000840	0.000125	0.001781	0.000658	0.000995	0.000096	0.000434	0.000671	0.000938	0.000123	0.001539
Nucleolus	0.004276	0.005292	0.001071	0.001670	0.002442	0.004066	0.001446	0.003358	0.003419	0.004518	0.001097	0.001973
Nucleus	0.780602*	0.876550*	0.932268*	0.811169*	0.869742*	0.865947*	0.978986*	0.629317*	0.822248*	0.869858*	0.935493*	0.778495*
Peroxisomes	0.001459	0.002006	0.000153	0.000470	0.001716	0.002907	0.000098	0.007145	0.001579	0.002575	0.000150	0.001669
Vacuole	0.003333	0.005101	0.004223	0.023568	0.002489	0.007724	0.004167	0.024359	0.002938	0.006757	0.004219	0.023710
Vacuole Periphery	0.000650	0.000260	0.000368	0.008463	0.000239	0.000849	0.000050	0.003464	0.000458	0.000631	0.000346	0.007565

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-7.49	-8.38	-9.90	-6.64	-7.00	-1.60	-5.53	-7.63	-0.12	-2.04	-1.98	-6.64	-10.80	-1.03	-7.80
Bud Neck	-2.98	-12.77	-13.40	-10.58	-2.31	-6.96	-3.43	-4.23	-1.60	-3.69	-7.24	-13.62	-14.34	-8.71	-2.32
Bud Site	-0.50	-2.92	-6.21	-5.86	-4.48	-4.25	-1.41	-3.67	-2.46	-3.58	-3.43	-4.68	-7.92	-5.36	-5.27
Cell Periphery	4.88	4.15	4.34	-1.25	0.34	-1.45	5.12	-4.54	-3.83	-5.29	3.30	3.10	1.02	-2.65	-2.41
Cytoplasm	11.51	23.54	21.82	6.61	-2.31	6.21	19.05	-1.58	-4.67	-9.87	13.76	26.80	18.02	4.27	-7.18
Cytoplasmic Foci	4.89	12.55	10.64	3.82	-3.57	1.63	9.87	-0.93	-1.71	-4.68	5.29	15.73	9.29	3.89	-5.77
Eisosomes	3.04	8.76	0.00	-3.42	-10.97	-2.10	-2.72	-4.51	-4.11	-3.90	0.14	8.21	-4.76	-4.50	-8.02
Endoplasmic Reticulum	-4.64	-3.41	-13.18	-10.10	-13.49	-9.96	-9.15	-8.73	-4.79	0.24	-8.08	-7.75	-17.29	-12.60	-13.57
Endosome	-0.15	1.41	-5.02	-4.96	-5.53	-3.81	3.12	-3.43	-2.48	-3.72	-3.70	1.57	-6.14	-3.86	-6.54
Golgi	-0.58	2.58	-2.82	-2.51	-3.37	-3.11	1.93	-2.34	-1.75	-2.43	-3.13	2.84	-3.67	-2.01	-4.06
Lipid Particles	4.35	7.87	6.19	3.09	-3.33	-1.39	6.03	-0.72	-0.07	-1.87	0.65	10.07	4.14	2.54	-3.16
Mitochondria	0.63	2.19	-2.80	-3.02	-4.87	-2.91	2.59	-1.39	0.67	-2.75	-2.54	2.33	-3.22	-0.46	-5.36
Mitotic Spindle	-2.61	-3.97	-7.93	-4.45	-6.56	-4.47	1.06	-3.17	-1.09	-3.39	-5.29	-3.49	-8.42	-4.66	-7.05
None	6.53	8.74	8.87	2.31	0.62	4.23	7.47	-2.68	-3.38	-3.79	8.28	9.41	5.37	-1.86	-2.74
Nuclear Periphery	1.00	1.58	-4.61	-6.32	-6.95	-4.28	2.11	-5.24	0.65	-6.74	-2.37	1.33	-6.11	-4.42	-7.48
Nuclear Periphery Foci	-0.49	2.91	-2.05	-1.00	-4.60	-0.95	1.61	-1.27	-0.49	-2.43	-1.11	3.56	-2.37	-1.07	-5.21
Nucleolus	-0.92	5.83	4.72	3.15	-2.37	-2.50	3.27	0.00	1.49	-1.22	-1.69	7.24	4.47	4.56	-2.70
Nucleus	-9.42	-20.55	-5.09	4.52	13.57	0.70	-12.95	9.61	9.17	14.83	-6.84	-21.09	2.27	7.72	18.01
Peroxisomes	-0.96	5.35	4.62	3.59	-2.10	-1.57	3.31	-1.16	-0.26	-2.70	-2.10	5.39	2.23	4.06	-3.12
Vacuole	-2.29	-2.65	-13.70	-12.23	-12.93	-6.60	-3.05	-8.31	-7.50	-7.08	-6.61	-4.27	-16.01	-13.76	-14.86
Vacuole Periphery	1.29	0.98	-3.36	-3.95	-3.84	-3.15	3.37	-2.19	-1.27	-2.46	-0.84	0.74	-3.90	-3.73	-4.13