Standard name
Human Ortholog
Description Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0.14 0.16 0.11 0.12 0.1 0.07 0.07 0.06 0.05 0.12 0.24 0.27 0.22 0.27 0.23 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.38 0.29 0.21 0.28 0.4 0.72 0.69 0.59 0.62 0.75 0.72 0.77 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0.81 0.82 0.79 0.68 0.59 0.61 0.55 0.66 0.67 0.61 0.66 0.63 0.84 0.71 0.66 0.67 0.58 0.52 0.8 0.8 0.72 0.71 0.61 0.52
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0.05 0 0.09 0.05 0 0 0 0 0 0 0 0.08 0.06 0.16 0.21 0.25 0.13 0.11 0.15 0.15 0.22 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1
Bud 1 0 0 0 4 3 4 4 3 5 4 3 0 0 0 0 0 1 2 4 3 3 3 3
Bud Neck 1 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 1 1 0 0 0 1
Bud Site 0 0 0 1 2 8 3 10 9 17 19 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 17 12 25 21 33 27 30 38 32 34 24 22 38 85 103 19 26 26 6 9 8 18 9 12
Endoplasmic Reticulum 1 1 0 0 0 0 0 0 0 0 0 0 1 2 1 5 3 6 0 0 1 0 0 1
Endosome 0 0 1 2 6 0 0 0 3 1 0 0 5 3 6 1 0 3 6 2 7 2 3 4
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0 5 0 0 3
Mitochondria 177 95 68 42 84 181 174 231 278 347 272 325 0 4 8 2 6 5 3 4 4 11 7 11
Nucleus 383 274 253 101 124 153 140 257 302 282 249 266 273 257 252 58 56 59 401 281 266 276 159 151
Nuclear Periphery 1 0 0 1 1 1 0 1 4 1 3 5 0 1 2 0 0 1 1 0 1 0 1 3
Nucleolus 0 0 0 0 3 1 0 3 3 5 5 8 0 0 0 0 0 0 1 0 0 3 5 10
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
SpindlePole 0 1 0 3 2 0 0 2 1 1 4 0 1 0 3 0 1 0 3 5 7 8 6 9
Vac/Vac Membrane 16 20 16 5 19 12 9 12 18 17 9 16 12 29 23 14 20 29 67 37 56 58 56 67
Unique Cell Count 471 333 321 148 210 251 253 391 449 464 380 424 324 361 380 87 97 114 504 353 369 388 260 290
Labelled Cell Count 597 403 365 176 278 386 360 558 654 710 589 655 331 383 399 99 112 131 504 353 369 388 260 290


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.5 4.6 4.5 4.5 3.8 3.9 3.9 3.8 3.5 3.3 3.4 6.2 6.1 5.6 7.0 6.7 6.8 6.3 6.5 6.7
Std Deviation (1e-4) 0.5 0.7 1.2 1.9 1.8 1.7 2.0 1.9 1.4 1.1 1.3 1.3 1.2 1.5 1.2 1.4 1.5 1.5 1.6 1.8 1.7
Intensity Change (Log2) – – – -0.03 -0.04 -0.27 -0.24 -0.22 -0.27 -0.39 -0.49 -0.45 0.45 0.42 0.29 0.61 0.55 0.56 0.47 0.51 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.5 4.8 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6139 4.6274 4.6879 4.3121 4.1365 4.3461 2.4773 2.4851 2.5202 1.9407 1.905 2.3295 1.9644 2.1149 1.7613 1.8221 1.5441 2.1173
Actin 0.005 0.0002 0.0071 0.0011 0.001 0.006 0.0033 0 0.004 0.0045 0 0 0.0018 0 0 0 0 0
Bud 0.0006 0.0002 0.0002 0.0004 0.0023 0.0003 0.0001 0 0.0001 0.0001 0 0 0.0009 0 0 0 0 0
Bud Neck 0.0033 0.0004 0.0008 0.0011 0.0004 0.0041 0.0018 0.0002 0.0006 0.0008 0.0018 0.0008 0.004 0.0001 0.0001 0 0.0003 0.0004
Bud Periphery 0.0016 0.0001 0.0006 0.0026 0.0009 0.0011 0.0002 0.0001 0.0001 0.0002 0 0 0.0002 0 0 0 0 0
Bud Site 0.0074 0.0011 0.0009 0.0009 0.0004 0.0004 0.0002 0.0003 0.0003 0.0019 0.0001 0 0.0003 0.0001 0 0 0 0
Cell Periphery 0.002 0 0.0006 0.0003 0.0003 0.0002 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.0069 0.0102 0.013 0.007 0.033 0.0035 0.0015 0.0049 0.0003 0.0002 0.0026 0.0002 0.0029 0.0041 0.0033 0.0001 0.0003 0.0045
Cytoplasmic Foci 0.0147 0.0004 0.0014 0.0003 0.0072 0.0011 0.0019 0.0001 0.0002 0.0043 0.0001 0 0.0015 0 0.0001 0 0 0.0032
Eisosomes 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0045 0.0005 0.0045 0.0005 0.002 0.0013 0.0016 0.0001 0.0002 0 0.0001 0 0.0003 0.0004 0.0004 0 0 0
Endosome 0.0145 0.0005 0.0025 0.0046 0.007 0.0027 0.0075 0.0002 0.0009 0.0011 0.0004 0 0.0025 0.0002 0.0003 0 0 0
Golgi 0.0035 0 0.0023 0.0002 0.0006 0.0012 0.0014 0 0.0003 0.0014 0 0 0.0004 0 0 0 0 0
Lipid Particles 0.0097 0.0001 0.0005 0.0002 0.0062 0.0014 0.0079 0 0.0002 0.0027 0 0 0.0011 0 0.0001 0 0 0
Mitochondria 0.0159 0.0003 0.0019 0.018 0.0028 0.001 0.0022 0.0001 0.0002 0.0005 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0 0
None 0.0067 0.0116 0.0219 0.0124 0.0358 0.0006 0.0016 0.0027 0.0002 0.0002 0.0002 0 0.0029 0.0029 0.0026 0 0.0001 0.0009
Nuclear Periphery 0.0344 0.0058 0.0089 0.0076 0.0083 0.0055 0.009 0.0036 0.0039 0.001 0.0021 0.0011 0.0047 0.0068 0.0031 0.0003 0.0014 0.0013
Nucleolus 0.0079 0.0068 0.006 0.0161 0.0109 0.0132 0.0058 0.0055 0.0042 0.0032 0.0081 0.0085 0.0031 0.0029 0.0021 0.0032 0.008 0.0057
Nucleus 0.8035 0.9367 0.8742 0.8989 0.8515 0.9395 0.9407 0.9775 0.9793 0.9687 0.9798 0.9828 0.9555 0.9783 0.9861 0.9961 0.9849 0.9747
Peroxisomes 0.0077 0 0.0002 0.0001 0.0009 0.0002 0.0009 0 0.0001 0.007 0 0 0.0004 0 0 0 0 0
Punctate Nuclear 0.0414 0.0234 0.0509 0.004 0.0061 0.0151 0.0098 0.004 0.0047 0.0018 0.0042 0.0063 0.0157 0.0035 0.0015 0.0002 0.0048 0.0091
Vacuole 0.0055 0.0013 0.0013 0.0207 0.0175 0.0012 0.0013 0.0005 0.0001 0.0001 0.0004 0.0001 0.0009 0.0004 0.0002 0 0.0001 0.0001
Vacuole Periphery 0.0032 0.0002 0.0003 0.0029 0.0049 0.0003 0.0011 0.0001 0.0001 0 0 0 0.0002 0.0001 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.8289 7.914 9.0758 7.7367 8.3478 4.8262 8.5475 5.1552 8.7137 7.4451
Translational Efficiency 1.2664 0.8441 0.7122 0.7905 0.5579 1.1917 0.7604 1.2113 0.77 0.7952

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member
Localization
Cell Percentages nucleus (75%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Esc2

Esc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Esc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available