TheCellVision

YPR1

Standard name	YPR1 ORF YDR368W Aliases trifunctional aldehyde reductase/carbonyl reductase (NADPH)/glucose 1-dehydrogenase (NADP(+)) YPR1
Description	NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication; human homolog AKR1B1 can complement yeast null mutant

Standard name

YPR1 ORF YDR368W Aliases trifunctional aldehyde reductase/carbonyl reductase (NADPH)/glucose 1-dehydrogenase (NADP(+)) YPR1

Description

NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication; human homolog AKR1B1 can complement yeast null mutant

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Scale images

YPR1

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.05	0	0	0	0	0	0
Cytoplasm	0.99	0.99	1.0	0.98	0.96	0.89	0.83	0.72	0.73	0.69	0.72	0.66	1.0	0.98	0.99	0.98	0.97	0.98	0.98	0.97	0.96	0.98	0.97	0.95
Endoplasmic Reticulum	0	0	0	0	0	0	0	0.05	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0	0	0	0	0	0.06	0.07	0.12	0.05	0	0	0	0	0	0	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	0	0	0	0	0.08	0.13	0.19	0.17	0.2	0.14	0.25	0	0	0	0	0	0	0	0	0	0	0	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	4	2	1	0	8	0	0	0	0	0	0	0	0	0	0	0	1
Bud	2	0	1	0	0	0	1	1	3	3	6	11	0	0	0	1	0	0	0	2	0	0	2	5
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	1	3	0	6	8	11	15	14	9	15	14	5	4	9	6	14	19	0	0	0	0	0	0
Cytoplasm	259	182	138	306	450	346	472	418	309	307	253	341	448	589	693	368	390	354	247	177	145	431	585	627
Endoplasmic Reticulum	1	0	0	1	6	8	12	28	13	13	4	12	0	2	3	7	8	11	1	0	0	1	2	2
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	1	0	0	0	0	1	2	3
Golgi	1	0	0	0	0	2	0	4	0	0	0	1	0	4	0	0	2	1	1	0	0	0	2	3
Mitochondria	2	1	0	1	1	7	18	18	27	29	43	24	0	0	0	1	1	0	0	0	0	0	0	2
Nucleus	0	1	2	2	3	3	10	14	8	19	7	13	1	3	3	0	1	1	0	1	2	2	3	2
Nuclear Periphery	0	0	0	0	0	0	0	2	1	3	0	0	0	0	0	0	0	0	0	0	0	0	0	1
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	1	0	0	11	21	31	72	113	73	87	51	129	2	6	2	7	7	4	0	0	1	1	2	3
Unique Cell Count	261	183	138	313	470	388	570	583	424	442	352	515	450	600	698	375	401	361	253	183	151	442	607	658
Labelled Cell Count	266	185	144	321	487	405	596	617	450	471	379	554	456	609	710	391	424	390	253	183	151	442	607	658

Yeast GFP Assignment

Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	11.0	13.3	13.1	11.9	11.9	10.8	10.5	10.8	9.9	9.8	9.7	10.5	16.7	18.0	19.4	32.1	35.4	35.0	13.6	14.6	15.3
Std Deviation (1e^-4)	1.8	2.3	1.9	2.1	2.0	1.8	1.9	2.2	2.1	2.2	2.4	2.6	3.1	3.7	3.9	8.8	10.9	10.7	2.1	2.6	2.7
Intensity Change (Log₂)	–	–	–	-0.14	-0.13	-0.28	-0.32	-0.28	-0.4	-0.42	-0.42	-0.32	0.36	0.46	0.57	1.3	1.44	1.42	0.06	0.16	0.23

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cell Periphery	0	0	0	0	0	0	0	1.1	0	0	0	0	0	0	1.5
Cytoplasm	-1.8	-2.5	-4.0	-5.2	-7.1	-6.9	-7.4	-7.0	-8.0	-0.8	-1.6	-1.0	-1.6	-2.0	-1.6
Endoplasmic Reticulum	0	0	0	0	2.6	0	0	0	0	0	0	0	0	0	0
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0	0	3.0	3.1	4.3	2.6	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vacuole	0	0	3.4	4.4	5.6	5.2	5.7	4.7	6.6	0	0	0	0	0	0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	9.7616	11.4986	11.057	8.879	9.5717	10.1821	8.9188	11.5424	10.6318	9.8568	7.9218	10.5161	10.5971	10.7436	11.7391	10.623	10.285	12.33
Actin	0.0002	0.0004	0.0017	0.0005	0.0006	0.0003	0.0126	0.0018	0.0076	0.0192	0.0009	0.0007	0.0103	0.0009	0.0014	0.0029	0.0585	0.0004
Bud	0.0001	0.0002	0.0038	0.0075	0.0002	0.0008	0.0012	0.0003	0.0019	0.0003	0.0002	0.0002	0.0008	0.0086	0.0018	0.0002	0.0001	0.0001
Bud Neck	0.0002	0.0002	0.0003	0.0014	0.001	0.0021	0.0025	0.0002	0.001	0.0009	0.0006	0.0013	0.001	0.0002	0.0004	0.0005	0.0005	0.0006
Bud Periphery	0	0	0.0006	0.003	0.0001	0.0002	0.0007	0.0001	0.0009	0.0004	0.0001	0.0001	0.0005	0.0012	0.0004	0.0001	0.0001	0.0001
Bud Site	0.0001	0.0006	0.0009	0.0007	0.0002	0.0003	0.0024	0.0011	0.0027	0.0003	0.0005	0.0001	0.001	0.0021	0.0022	0.0002	0.0002	0
Cell Periphery	0	0	0.0001	0.0001	0	0	0.0001	0	0.0001	0.0001	0.0001	0	0.0003	0.0002	0.0001	0.0001	0	0
Cytoplasm	0.9326	0.9698	0.948	0.8931	0.9184	0.9202	0.8122	0.9755	0.9245	0.8752	0.7045	0.948	0.8982	0.9554	0.9605	0.9064	0.8944	0.9593
Cytoplasmic Foci	0.0037	0.002	0.0022	0.0078	0.0023	0.0077	0.0419	0.0033	0.0111	0.0144	0.0381	0.0052	0.0079	0.0028	0.0033	0.0044	0.0014	0.0014
Eisosomes	0	0	0	0	0	0	0.0001	0	0	0.0004	0	0	0.0001	0	0	0.0001	0.0002	0
Endoplasmic Reticulum	0.0054	0.0018	0.001	0.004	0.0024	0.0022	0.0049	0.0007	0.0025	0.004	0.0063	0.0013	0.0033	0.0011	0.0009	0.0035	0.0011	0.0015
Endosome	0.0052	0.0009	0.0011	0.0179	0.0012	0.003	0.0366	0.0006	0.0106	0.0232	0.169	0.004	0.0089	0.0009	0.0051	0.0123	0.0009	0.0041
Golgi	0.0001	0.0001	0.0005	0.0008	0	0.0002	0.0076	0.0003	0.0023	0.004	0.0134	0.0019	0.0015	0.0001	0.001	0.0019	0.0018	0.0003
Lipid Particles	0.0002	0	0.0011	0.0001	0	0.0004	0.0134	0.0001	0.0029	0.0034	0.0211	0.0009	0.0015	0	0.0005	0.0022	0.0012	0.0001
Mitochondria	0.0002	0.0001	0.0053	0.0005	0.0004	0.0002	0.0034	0.0069	0.0015	0.0066	0.0046	0.0005	0.0055	0.0003	0.0004	0.0028	0.0006	0.0004
None	0.0058	0.0014	0.0016	0.0034	0.0032	0.0017	0.0102	0.0012	0.0039	0.0021	0.0025	0.0015	0.0031	0.0017	0.0012	0.0021	0.0006	0.0007
Nuclear Periphery	0.0057	0.0027	0.0025	0.0062	0.006	0.005	0.006	0.0008	0.0029	0.0041	0.0068	0.0032	0.0077	0.0037	0.0024	0.0231	0.0042	0.008
Nucleolus	0.0001	0	0.0001	0.0001	0.0001	0.0001	0.0006	0	0.0002	0.0001	0.0005	0.0002	0.0048	0.0001	0.0001	0.0002	0	0.0001
Nucleus	0.0373	0.0181	0.0259	0.0452	0.0615	0.0511	0.0155	0.0055	0.0151	0.0212	0.0155	0.0262	0.0331	0.018	0.0161	0.027	0.0281	0.0205
Peroxisomes	0.0001	0.0001	0.0019	0.0002	0	0.0002	0.0147	0.0007	0.0037	0.011	0.0016	0.0002	0.001	0.0002	0.0006	0.0002	0.0028	0
Punctate Nuclear	0.001	0.0006	0.0006	0.002	0.0014	0.003	0.0091	0.0005	0.002	0.0013	0.0026	0.0038	0.006	0.0011	0.0009	0.0022	0.0028	0.0006
Vacuole	0.0018	0.0008	0.0007	0.0047	0.0007	0.001	0.0033	0.0002	0.0016	0.006	0.0081	0.0006	0.0027	0.0011	0.0006	0.0057	0.0003	0.0011
Vacuole Periphery	0.0003	0.0001	0.0002	0.0008	0.0002	0.0002	0.0011	0.0001	0.0006	0.0019	0.0029	0.0002	0.0006	0.0002	0.0001	0.0021	0.0001	0.0007

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	61.1044	35.7154	60.0201	65.2429	51.8007	76.3382	57.6196	61.2911	78.2062	67.4088
Translational Efficiency	2.0185	3.1839	1.7344	2.063	2.554	1.9952	2.2724	2.1042	1.876	1.9059

Hit Data

Dataset	Hit
Protein Abundance	✘
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘