Standard name
Description mRNA 5'-end-capping quality-control protein; has distributive, 5'-3' exoRNase activity; similar to Rai1p;
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.96 0.97 0.98 0.96 0.86 0.79 0.77 0.74 0.72 0.66 0.68 0.98 0.98 0.99 0.96 0.95 0.94 0.85 0.9 0.84 0.82 0.81 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0.09 0.12 0.14 0.38 0.44 0.4 0.53 0.5 0.55 0.54 0 0 0 0.09 0.06 0 0.07 0 0.07 0.05 0.05 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 1 1 4
Bud 0 0 0 0 2 12 12 15 14 4 22 18 0 0 0 0 1 0 0 0 2 3 7 5
Bud Neck 0 0 1 0 0 3 6 6 1 4 3 6 2 1 0 1 1 0 0 0 0 1 1 2
Bud Site 0 0 0 0 0 0 0 2 0 1 4 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 2 1 2 6 1 3 1 1 0 0 5 1 2 4 0 0 1 0 1 2
Cytoplasm 17 102 254 228 354 391 501 518 301 321 225 314 276 303 469 172 130 100 41 102 216 167 243 232
Endoplasmic Reticulum 0 2 0 0 0 1 1 1 0 0 1 3 1 2 3 12 8 7 0 0 0 3 2 5
Endosome 0 0 0 0 0 0 0 2 0 0 0 0 2 2 1 2 2 0 0 1 3 2 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1 2 24 29 52 172 275 272 215 220 185 251 0 0 0 16 8 4 3 4 16 9 15 27
Nucleus 0 0 0 1 1 2 0 6 3 2 3 2 1 0 0 2 0 0 0 0 0 1 1 0
Nuclear Periphery 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 1 1 1 2 4 2 2 1 1 0 1 0 0 1 1 0 0 0 0 2 0
Peroxisomes 0 0 0 0 1 0 0 2 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 5 6 5 6 11 8 9 0 0 1 0 3 0 2 0 3 2 2 2
Vac/Vac Membrane 0 0 0 1 1 1 2 1 4 1 0 3 2 1 2 2 0 2 0 0 2 5 10 15
Unique Cell Count 17 106 263 232 367 453 631 672 406 443 339 464 282 308 474 180 137 106 49 113 257 205 301 310
Labelled Cell Count 18 107 281 261 416 590 807 840 548 570 453 610 284 311 481 209 156 119 49 113 257 205 301 310


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.3 3.5 3.4 3.5 3.2 2.6 3.0 2.7 2.8 2.7 2.8 5.4 5.4 5.4 5.0 5.1 5.2 6.1 6.1 5.9
Std Deviation (1e-4) 0.3 0.7 0.8 1.2 1.5 1.2 1.1 1.4 1.3 1.4 1.2 1.4 1.4 1.8 1.6 1.4 1.1 1.3 2.3 2.1 1.8
Intensity Change (Log2) – – – -0.02 0.01 -0.1 -0.39 -0.22 -0.34 -0.32 -0.35 -0.31 0.64 0.63 0.64 0.53 0.55 0.58 0.83 0.81 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dxo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available