Standard name | |
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Description | mRNA 5'-end-capping quality-control protein; has distributive, 5'-3' exoRNase activity; similar to Rai1p; |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 1 | 4 |
Bud | 0 | 0 | 0 | 0 | 2 | 12 | 12 | 15 | 14 | 4 | 22 | 18 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 7 | 5 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 3 | 6 | 6 | 1 | 4 | 3 | 6 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 6 | 1 | 3 | 1 | 1 | 0 | 0 | 5 | 1 | 2 | 4 | 0 | 0 | 1 | 0 | 1 | 2 |
Cytoplasm | 17 | 102 | 254 | 228 | 354 | 391 | 501 | 518 | 301 | 321 | 225 | 314 | 276 | 303 | 469 | 172 | 130 | 100 | 41 | 102 | 216 | 167 | 243 | 232 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 3 | 1 | 2 | 3 | 12 | 8 | 7 | 0 | 0 | 0 | 3 | 2 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 2 | 0 | 0 | 1 | 3 | 2 | 1 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 2 | 24 | 29 | 52 | 172 | 275 | 272 | 215 | 220 | 185 | 251 | 0 | 0 | 0 | 16 | 8 | 4 | 3 | 4 | 16 | 9 | 15 | 27 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 6 | 3 | 2 | 3 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 4 | 2 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 2 | 5 | 6 | 5 | 6 | 11 | 8 | 9 | 0 | 0 | 1 | 0 | 3 | 0 | 2 | 0 | 3 | 2 | 2 | 2 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 4 | 1 | 0 | 3 | 2 | 1 | 2 | 2 | 0 | 2 | 0 | 0 | 2 | 5 | 10 | 15 |
Unique Cell Count | 17 | 106 | 263 | 232 | 367 | 453 | 631 | 672 | 406 | 443 | 339 | 464 | 282 | 308 | 474 | 180 | 137 | 106 | 49 | 113 | 257 | 205 | 301 | 310 |
Labelled Cell Count | 18 | 107 | 281 | 261 | 416 | 590 | 807 | 840 | 548 | 570 | 453 | 610 | 284 | 311 | 481 | 209 | 156 | 119 | 49 | 113 | 257 | 205 | 301 | 310 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 4.3 | 3.5 | 3.4 | 3.5 | 3.2 | 2.6 | 3.0 | 2.7 | 2.8 | 2.7 | 2.8 | 5.4 | 5.4 | 5.4 | 5.0 | 5.1 | 5.2 | 6.1 | 6.1 | 5.9 |
Std Deviation (1e-4) | 0.3 | 0.7 | 0.8 | 1.2 | 1.5 | 1.2 | 1.1 | 1.4 | 1.3 | 1.4 | 1.2 | 1.4 | 1.4 | 1.8 | 1.6 | 1.4 | 1.1 | 1.3 | 2.3 | 2.1 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.01 | -0.1 | -0.39 | -0.22 | -0.34 | -0.32 | -0.35 | -0.31 | 0.64 | 0.63 | 0.64 | 0.53 | 0.55 | 0.58 | 0.83 | 0.81 | 0.77 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |