Standard name
Description Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 – – 0.65 0.56 – 0.89 0.8 0.81 0.53 – 0.79 1.0 0.98 0.95 0.69 0.88 0.6 0.88 0.66 0.75 0.78 0.85 0.82
Endoplasmic Reticulum 0 – – 0 0 – 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Mitochondria 0 – – 0 0 – 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – – 0 0 – 0 0 0 0 – 0.05 0 0 0 0 0 0 0.07 0.22 0.1 0.12 0.07 0.06
Nuclear Periphery 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – – 0.47 0.54 – 0.1 0.14 0.21 0.43 – 0.18 0 0 0.06 0.43 0.06 0.4 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – – 0 0 – 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – – 0 0 – 0 0 0 0 – 1 1 0 0 0 0 0 0 2 0 0 0 3
Bud Neck 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – – 0 0 – 0 0 1 0 – 0 0 0 1 0 0 3 0 0 1 0 0 0
Cytoplasm 117 – – 11 40 – 92 64 34 32 – 31 116 119 118 34 15 36 110 42 22 63 105 147
Endoplasmic Reticulum 0 – – 0 0 – 0 4 0 0 – 1 0 0 1 1 0 1 0 0 0 0 1 1
Endosome 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 1 1 2 0 0 0 0 0 1
Mitochondria 1 – – 0 1 – 1 2 1 3 – 0 0 1 0 1 0 1 1 1 0 0 1 1
Nucleus 0 – – 0 0 – 0 0 1 1 – 2 0 1 0 0 0 1 8 14 2 9 8 10
Nuclear Periphery 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – – 0 0 – 2 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – – 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 – – 8 38 – 10 11 9 26 – 7 1 1 7 21 1 24 2 2 0 3 0 5
Unique Cell Count 119 17 71 103 80 42 60 39 116 121 124 49 17 60 126 65 30 81 124 179
Labelled Cell Count 122 19 79 105 82 46 62 42 118 122 127 58 17 68 126 65 30 81 124 179


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 – – 11.8 12.1 – 11.6 12.1 12.2 9.7 – 10.9 13.5 13.5 12.9 14.8 15.9 15.5 10.5 11.7 13.1
Std Deviation (1e-4) 1.8 – – 1.4 1.9 – 2.7 2.8 2.7 2.3 – 2.5 2.0 1.8 2.0 3.2 2.6 4.7 2.0 2.3 3.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.6714 14.9788 12.8277 10.8387 10.7638 12.7733 11.1981 15.0242 13.4358 12.8596 12.3887 12.9551 8.2638 12.7851 11.2947 9.6772 9.6919 11.7823
Actin 0.0145 0.0011 0.0122 0.0036 0.0205 0.002 0.0024 0.0001 0.0001 0.0001 0.0009 0.0001 0.0495 0.0004 0.0126 0.0945 0.0035 0.0003
Bud 0.0003 0.0003 0.0005 0.0006 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0009 0.0004 0.0003 0.0003 0.0003 0.0001
Bud Neck 0.0007 0.0005 0.0008 0.0014 0.001 0.0022 0.0006 0.0004 0.0003 0.0006 0.0009 0.0018 0.0013 0.0003 0.0006 0.0005 0.0006 0.0013
Bud Periphery 0.0004 0.0001 0.0007 0.0005 0.0002 0.0001 0.0001 0 0 0 0.0001 0 0.0009 0.0001 0.0002 0.0002 0.0002 0
Bud Site 0.0014 0.0018 0.0012 0.0014 0.0004 0.0003 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0054 0.0009 0.001 0.0012 0.0005 0
Cell Periphery 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0 0 0 0 0 0 0.0004 0.0001 0.0001 0 0 0
Cytoplasm 0.2444 0.4065 0.2457 0.2826 0.1567 0.2741 0.2557 0.3649 0.2624 0.3351 0.3185 0.2425 0.2231 0.5558 0.4925 0.3409 0.3173 0.366
Cytoplasmic Foci 0.0083 0.0044 0.0075 0.0125 0.0089 0.0051 0.0034 0.0007 0.0002 0.0006 0.0019 0.0044 0.0325 0.0091 0.0049 0.0007 0.0274 0.0002
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0 0.0004 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0063 0.0009 0.0045 0.0127 0.0051 0.0029 0.0029 0.0007 0.0007 0.0004 0.0015 0.0016 0.007 0.0015 0.0062 0.0006 0.002 0.0008
Endosome 0.0343 0.0024 0.0158 0.0695 0.0622 0.0146 0.0043 0.0004 0.0003 0.0002 0.0199 0.0022 0.0385 0.0172 0.0124 0.0003 0.0924 0.0002
Golgi 0.0072 0.0015 0.0062 0.0047 0.0418 0.0126 0.0004 0 0 0 0.0035 0.0008 0.0216 0.0016 0.0103 0.0012 0.0809 0
Lipid Particles 0.0055 0.0007 0.0039 0.0057 0.0037 0.0057 0.0014 0 0 0 0.0067 0.0003 0.0233 0.0018 0.0022 0.0002 0.0048 0
Mitochondria 0.0058 0.001 0.0043 0.0062 0.0446 0.0177 0.0005 0.0002 0.0002 0.0001 0.0066 0.0002 0.0173 0.0006 0.0038 0.0014 0.004 0.0002
None 0.0013 0.0002 0.0004 0.0015 0.0005 0.0003 0.0013 0.0006 0.0002 0.0004 0.0005 0.0003 0.0071 0.0007 0.0047 0.0003 0.0004 0.0002
Nuclear Periphery 0.0337 0.0177 0.0192 0.0175 0.0208 0.0183 0.027 0.0117 0.0153 0.0101 0.0134 0.0204 0.0362 0.0139 0.0182 0.0153 0.0264 0.0226
Nucleolus 0.0005 0.0001 0.0003 0.0006 0.0002 0.0003 0.0004 0.0002 0.0003 0.0001 0.0002 0.0003 0.0018 0.0002 0.0001 0.0002 0.0002 0.0002
Nucleus 0.6233 0.5582 0.6607 0.5647 0.6256 0.6362 0.6881 0.6169 0.7172 0.6501 0.6202 0.72 0.5001 0.3909 0.4174 0.5392 0.43 0.6056
Peroxisomes 0.0025 0.0006 0.0103 0.0029 0.0014 0.0021 0.0023 0 0 0 0.0008 0.0004 0.0165 0.0002 0.0093 0.0009 0.002 0
Punctate Nuclear 0.0031 0.0011 0.0025 0.0031 0.002 0.0027 0.0073 0.0025 0.002 0.0018 0.0022 0.003 0.0114 0.0018 0.0018 0.0015 0.0032 0.0017
Vacuole 0.0047 0.0007 0.0025 0.006 0.0024 0.0018 0.0013 0.0005 0.0004 0.0002 0.0013 0.0013 0.0032 0.0022 0.0007 0.0003 0.0024 0.0004
Vacuole Periphery 0.0014 0.0002 0.0007 0.0023 0.0014 0.0005 0.0003 0.0001 0.0001 0.0001 0.0009 0.0002 0.0016 0.0003 0.0006 0.0002 0.0013 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 89.8244 66.5186 71.219 67.329 60.6425 90.3058 67.4971 73.9272 58.3918 69.4003
Translational Efficiency 1.1911 1.4118 1.1682 1.3732 1.3072 1.3205 1.3076 1.1433 1.4412 1.1875

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘