Standard name
Description Calmodulin-binding actin-associated protein; roles in endocytic membrane tabulation and constriction, and exocytosis; N-BAR domain protein that interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.73 0.59 – 0.3 0.38 0.25 0.29 0.4 0.39 0.39 0.14 0.4 0.47 0.43 0.49 0.47 0.62 0.61 0.78 0.72 0.34 0.52 0.25 0.16
Bud 0.23 0.24 – 0.13 0.19 0.13 0.21 0.14 0.24 0.18 0.19 0.15 0.15 0.16 0.18 0.14 0.14 0.17 0 0.08 0 0.07 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0
Bud Site 0.08 0 – 0 0 0 0 0.05 0 0.09 0.14 0 0.06 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.09 – 0 0 0.06 0 0 0.09 0 0 0.07 0.06 0.16 0.16 0.09 0.1 0.11 0 0 0 0 0 0
Cytoplasm 0.16 0.18 – 0.43 0.4 0.49 0.38 0.3 0.18 0.15 0.14 0.16 0.41 0.42 0.28 0.36 0.29 0.17 0 0.05 0.09 0.15 0.54 0.58
Endoplasmic Reticulum 0.06 0.1 – 0.13 0.08 0.13 0.06 0.16 0.05 0.07 0.19 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0.09 0.06 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0.05 0.08 0.06 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0.1 0 0 0.07 0.06 0.14 0.1 0.33 0 0 0 0 0 0
Mitochondria 0.18 0.3 – 0.13 0.13 0.23 0.28 0.36 0.45 0.56 0.48 0.53 0 0 0 0.08 0.1 0.22 0 0 0.25 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.16 0 – 0.17 0.09 0 0.13 0.14 0.07 0.13 0.19 0.12 0 0.07 0.14 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 115 54 – 7 32 18 34 54 40 33 3 36 55 94 116 40 13 11 149 80 12 61 47 33
Bud 36 22 – 3 16 9 25 19 24 15 4 14 17 34 42 12 3 3 7 9 1 8 4 8
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 5 2 4 3 5
Bud Site 13 4 – 0 1 0 0 7 2 8 3 3 7 0 1 0 0 0 – – – – – –
Cell Periphery 7 8 – 1 3 4 3 3 9 3 0 6 7 35 39 8 2 2 1 0 0 0 3 3
Cytoplasm 26 16 – 10 34 35 45 40 18 13 3 15 47 93 66 31 6 3 5 5 3 17 101 120
Endoplasmic Reticulum 9 9 – 3 7 9 7 21 5 6 4 4 4 16 12 2 0 0 1 0 0 3 3 6
Endosome 4 0 – 2 5 1 2 6 2 1 0 0 2 3 18 5 0 0 5 5 2 6 6 5
Golgi 1 4 – 0 1 1 2 6 1 1 2 3 4 16 15 12 2 6 3 0 0 1 0 1
Mitochondria 28 27 – 3 11 16 33 49 46 48 10 48 5 1 10 7 2 4 5 0 9 1 7 4
Nucleus 1 0 – 0 0 2 3 3 0 0 0 0 0 1 2 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 – 0 0 1 2 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 2 – 0 0 0 1 1 1 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 26 4 – 4 8 1 15 19 7 11 4 11 5 15 34 3 0 0 4 1 0 1 0 2
SpindlePole 0 0 – 0 1 0 3 6 2 3 0 2 1 1 9 4 0 0 2 0 0 1 0 0
Vac/Vac Membrane 1 0 – 0 1 1 2 1 1 1 0 1 3 0 2 0 0 0 0 2 2 3 8 8
Unique Cell Count 158 91 23 85 71 119 135 102 85 21 91 116 219 239 86 21 18 192 113 37 118 190 208
Labelled Cell Count 268 151 33 120 98 177 236 159 143 33 144 159 309 368 125 28 29 192 113 37 118 190 208


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 10.4 – 7.5 7.8 7.5 7.1 6.9 7.1 7.0 6.4 6.9 11.1 11.6 11.5 13.5 15.2 16.3 10.0 10.9 12.5
Std Deviation (1e-4) 1.5 1.9 – 1.0 1.8 1.8 2.0 1.9 1.7 1.9 1.6 2.4 2.2 1.8 2.4 3.7 3.9 2.8 2.2 2.2 2.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.8059 10.2482 9.6954 8.8982 9.4239 10.0235 6.1774 6.5023 6.199 6.1329 5.2026 6.9984 9.2661 10.7398 9.6769 9.2693 8.8923 10.0893
Actin 0.7032 0.5346 0.7163 0.7432 0.7455 0.8083 0.6944 0.4626 0.7246 0.7838 0.6812 0.8037 0.8025 0.745 0.8172 0.8925 0.8482 0.8692
Bud 0.0027 0.0048 0.0347 0.0188 0.006 0.0021 0.0009 0.0033 0.048 0.003 0.01 0.0004 0.0026 0.0033 0.0068 0.0045 0.0085 0.0005
Bud Neck 0.0103 0.0089 0.0118 0.0023 0.0214 0.0881 0.0516 0.0062 0.017 0.001 0.0015 0.0527 0.0059 0.0056 0.0087 0.0052 0.0004 0.0754
Bud Periphery 0.0042 0.0024 0.0372 0.0225 0.0042 0.0032 0.0008 0.0022 0.0576 0.0026 0.0227 0.0003 0.0023 0.0023 0.0063 0.0073 0.0307 0.0005
Bud Site 0.0204 0.1488 0.0361 0.0157 0.0042 0.0041 0.0521 0.1754 0.0354 0.0018 0.0029 0.0009 0.0188 0.0687 0.0248 0.0035 0.0021 0.0007
Cell Periphery 0.0046 0.0017 0.0008 0.0012 0.0003 0.0003 0.0016 0.0036 0.0014 0.0002 0.0005 0.0002 0.0005 0.0004 0.0007 0.0003 0.0005 0.0002
Cytoplasm 0.0574 0.1903 0.0678 0.0652 0.0774 0.03 0.014 0.3053 0.0672 0.1861 0.0271 0.0728 0.0202 0.082 0.0746 0.014 0.0397 0.0075
Cytoplasmic Foci 0.0478 0.0515 0.0334 0.0179 0.0534 0.0276 0.0903 0.0109 0.0216 0.0053 0.1143 0.0524 0.0378 0.0475 0.0171 0.0143 0.0039 0.0147
Eisosomes 0.0007 0.0002 0.0005 0.0004 0.0006 0.0004 0.0013 0.002 0.0012 0.0002 0.0002 0.0004 0.0006 0.0002 0.0003 0.0007 0.0003 0.0003
Endoplasmic Reticulum 0.0103 0.0009 0.0013 0.0045 0.0037 0.001 0.0012 0.0026 0.0006 0.0025 0.0238 0.0008 0.0015 0.0003 0.0023 0.0005 0.0155 0.002
Endosome 0.0274 0.0027 0.0084 0.0235 0.0203 0.0095 0.0053 0.003 0.0019 0.0023 0.0143 0.0017 0.0182 0.0055 0.0062 0.007 0.0089 0.0034
Golgi 0.0222 0.0088 0.0163 0.0101 0.02 0.0126 0.0048 0.0154 0.0072 0.0055 0.0151 0.0053 0.0242 0.0071 0.0055 0.0141 0.0061 0.0058
Lipid Particles 0.0118 0.0006 0.0023 0.0017 0.0113 0.001 0.0039 0.0023 0.0007 0.0005 0.0055 0.0006 0.0127 0.0003 0.0076 0.0069 0.0011 0.0015
Mitochondria 0.007 0.0007 0.0011 0.0084 0.0027 0.0009 0.0013 0.0012 0.0043 0.0016 0.0017 0.0003 0.0135 0.0003 0.0007 0.0197 0.0191 0.006
None 0.0047 0.0004 0.0026 0.0014 0.001 0.0004 0.0357 0.0006 0.0003 0.0002 0.0012 0.0001 0.0015 0.0003 0.0033 0.0004 0.0015 0.0003
Nuclear Periphery 0.0168 0.0002 0.0053 0.0104 0.0019 0.0002 0.0008 0.0002 0.0001 0.0001 0.0016 0 0.0018 0.0001 0.0003 0.0002 0.0054 0.0006
Nucleolus 0.0006 0 0.0011 0.0004 0.0001 0.0001 0.003 0.0001 0 0 0 0 0.0003 0 0.0001 0.0001 0.0001 0
Nucleus 0.0039 0.0003 0.0025 0.0039 0.0011 0.0004 0.003 0.0002 0.0001 0.0001 0.0003 0 0.0005 0.0001 0.0003 0.0001 0.0009 0.0003
Peroxisomes 0.0311 0.0415 0.0162 0.031 0.0219 0.0091 0.0075 0.002 0.0103 0.0025 0.0711 0.0069 0.0294 0.0304 0.0162 0.0075 0.0039 0.0105
Punctate Nuclear 0.0075 0.0005 0.0036 0.0013 0.0024 0.0003 0.0263 0.0003 0.0003 0.0001 0.0046 0.0004 0.0038 0.0003 0.0007 0.0004 0.0008 0.0003
Vacuole 0.0034 0.0002 0.0004 0.0103 0.0004 0.0003 0.0003 0.0003 0.0002 0.0005 0.0003 0 0.0009 0.0002 0.0002 0.0003 0.0016 0.0001
Vacuole Periphery 0.0022 0 0.0002 0.0059 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0002 0 0.0005 0 0.0001 0.0003 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 138.5919 106.8744 133.8595 121.4682 150.5648 143.8426 106.512 108.4714 146.2208 119.3087
Translational Efficiency 1.375 1.4103 1.1545 1.4564 1.1739 1.2391 1.2677 1.5371 1.2212 1.3014

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘