Standard name
Human Ortholog
Description Xanthine-guanine phosphoribosyl transferase; required for xanthine utilization and for optimal utilization of guanine
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 1.0 0.97 0.85 0.82 0.79 – 0.63 0.69 0.66 0.61 0.98 0.99 0.98 0.95 0.95 0.95 0.93 0.86 0.94 0.94 0.87 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0 – 0.14 0.14 0.21 0.2 0 0 0 0 0 0 0 0.05 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.19 0.12 0.18 – 0.22 0.22 0.16 0.21 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 4 4
Bud 0 1 1 1 0 2 0 – 0 1 1 5 0 0 0 0 0 0 4 0 1 2 10 9
Bud Neck 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 1 0 0 2 – 1 3 4 16 1 0 2 4 8 6 0 0 0 0 0 1
Cytoplasm 253 89 17 60 148 69 188 – 59 115 108 147 237 413 408 366 228 297 235 80 46 195 375 339
Endoplasmic Reticulum 0 0 0 0 1 0 8 – 3 3 1 0 0 1 0 8 6 5 1 1 0 0 2 6
Endosome 2 0 0 0 1 0 0 – 0 0 1 1 0 2 2 1 1 2 0 1 0 0 4 13
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 2 1 0 1 0 0 0 3 3
Mitochondria 0 4 0 0 0 8 7 – 13 23 34 48 0 0 0 0 1 3 1 4 0 1 9 14
Nucleus 2 1 0 0 2 1 8 – 4 3 9 14 2 1 1 7 4 3 0 0 0 0 0 2
Nuclear Periphery 0 1 0 0 1 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 1 3 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 1 2 0 0 0 9 8
Vac/Vac Membrane 3 0 0 3 33 10 43 – 20 37 26 51 2 4 12 22 17 20 0 1 0 1 1 4
Unique Cell Count 259 92 17 62 174 84 239 93 167 164 242 241 419 416 386 241 314 252 93 49 207 431 418
Labelled Cell Count 262 96 20 65 188 90 256 100 186 184 282 244 421 425 410 267 340 252 93 49 207 431 418


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.1 6.3 7.1 10.5 6.8 9.8 – 8.2 8.2 7.7 8.4 7.2 6.8 6.5 10.9 11.3 11.1 6.1 6.2 6.3
Std Deviation (1e-4) 2.0 1.6 1.3 2.0 2.2 1.6 2.5 – 1.8 2.1 2.0 2.0 2.2 1.8 1.7 2.3 2.2 2.2 2.0 2.2 2.5
Intensity Change (Log2) – – – 0.18 0.74 0.13 0.65 – 0.39 0.4 0.3 0.42 0.2 0.13 0.05 0.8 0.85 0.83 -0.04 -0.01 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -1.7 -1.9 -2.1 – -3.0 -2.7 -2.9 -3.2 -0.5 -0.5 -0.6 -1.0 -1.0 -1.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4483 0.2066 -0.3217 -0.1954 -0.5642 -0.3929 0.3853 1.1545 0.9471 0.3037 0.786 1.0025 1.6406 2.6839 2.0266 2.7627 1.7072 2.4891
Actin 0.03 0.0004 0.0053 0.0165 0.0171 0.003 0.0089 0.0002 0.005 0.0331 0.031 0.0045 0.0018 0.0002 0.0004 0.0112 0.0122 0.0002
Bud 0.001 0.0003 0.0009 0.0004 0.0013 0.0021 0.0009 0.0003 0.0017 0.0003 0.0021 0.0002 0 0.0002 0.0004 0.0005 0.0002 0
Bud Neck 0.0024 0.0002 0.0025 0.0014 0.001 0.0053 0.003 0.0003 0.0005 0.0005 0.0012 0.0019 0.0005 0.0001 0.0001 0.0002 0.0002 0.0002
Bud Periphery 0.0016 0.0003 0.0006 0.0008 0.0034 0.0006 0.0012 0.0002 0.0014 0.0003 0.0025 0.0002 0 0.0002 0.0008 0.0006 0.0005 0
Bud Site 0.0051 0.0018 0.0013 0.0011 0.0037 0.0004 0.0095 0.0102 0.0024 0.0006 0.0009 0.0001 0.0001 0.0026 0.0004 0.0005 0.0001 0
Cell Periphery 0.0004 0.0001 0.0001 0.0001 0.0003 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.3894 0.6148 0.4562 0.4966 0.4562 0.5119 0.4166 0.5781 0.495 0.4587 0.567 0.6148 0.1779 0.3591 0.319 0.2662 0.3257 0.382
Cytoplasmic Foci 0.0455 0.0097 0.0188 0.0231 0.0166 0.0228 0.0268 0.0056 0.0123 0.0102 0.0224 0.0114 0.0026 0.0054 0.0047 0.0117 0.0069 0.0039
Eisosomes 0.0004 0 0.0001 0.0002 0.0007 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0.0004 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.0055 0.0031 0.0027 0.0052 0.0035 0.0021 0.0044 0.0018 0.0028 0.002 0.0027 0.0014 0.0022 0.0012 0.0015 0.0014 0.0013 0.0016
Endosome 0.0284 0.0043 0.0109 0.0225 0.02 0.0077 0.0201 0.0018 0.0119 0.0064 0.0255 0.0032 0.0013 0.0026 0.0008 0.0035 0.0018 0.0014
Golgi 0.0059 0.0002 0.0029 0.0087 0.0142 0.001 0.0038 0.0001 0.003 0.0047 0.01 0.0008 0.0003 0.0002 0.0001 0.0006 0.0003 0.0001
Lipid Particles 0.0097 0.0002 0.0031 0.0289 0.0099 0.0016 0.0099 0.0002 0.004 0.006 0.0103 0.0025 0.0003 0.0003 0.0001 0.0008 0.0001 0.0001
Mitochondria 0.0135 0.0004 0.0063 0.0052 0.0089 0.0007 0.0114 0.0001 0.0022 0.0027 0.0019 0.0004 0.0001 0.0001 0.0002 0.0005 0.0003 0.0001
None 0.4169 0.3546 0.4769 0.3339 0.3957 0.4217 0.4521 0.3951 0.4341 0.4561 0.3018 0.3432 0.8081 0.625 0.6679 0.6906 0.6467 0.607
Nuclear Periphery 0.0116 0.0014 0.0019 0.0057 0.0192 0.0022 0.006 0.0007 0.0072 0.0019 0.0026 0.0026 0.0018 0.0005 0.0005 0.0015 0.0005 0.0005
Nucleolus 0.0013 0.0003 0.0003 0.0013 0.0026 0.0003 0.0015 0.0001 0.0003 0.0001 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001 0
Nucleus 0.005 0.0036 0.0029 0.0045 0.0048 0.0072 0.0062 0.0025 0.0054 0.0102 0.0036 0.0054 0.0009 0.0008 0.0012 0.0016 0.0012 0.0017
Peroxisomes 0.0084 0.0002 0.0012 0.0345 0.0027 0.0011 0.0055 0.0001 0.0061 0.0021 0.0059 0.0025 0.0001 0.0001 0.0003 0.0023 0.0004 0.0001
Punctate Nuclear 0.0122 0.0013 0.0026 0.006 0.0093 0.0071 0.008 0.0009 0.0021 0.0024 0.0052 0.0034 0.0008 0.0006 0.0008 0.0038 0.0006 0.0005
Vacuole 0.0044 0.0025 0.0017 0.0022 0.0056 0.0009 0.0028 0.0014 0.0016 0.001 0.0025 0.0007 0.0003 0.0007 0.0004 0.0016 0.0004 0.0003
Vacuole Periphery 0.0014 0.0002 0.0006 0.0011 0.0033 0.0002 0.0008 0.0001 0.0006 0.0003 0.0008 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.0345 37.5762 45.3102 33.5944 17.0311 45.5121 38.7848 36.8617 37.3676 34.4526
Translational Efficiency 0.971 1.1194 0.8928 1.265 2.1093 0.9678 1.072 1.256 1.3567 1.4137

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
203 1570 2203 1664 1908 2438 98 1658 2111 4008 2301 3322

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 874.54 821.21 927.68 789.90 753.34 772.74 996.53 841.45 764.99 791.73 930.61 815.63
Standard Deviation 161.68 144.04 148.14 111.87 105.48 101.75 156.74 126.54 117.67 122.41 149.17 122.17
Intensity Change Log 2 — -0.090773 0.085103 -0.146854 — 0.036682 0.403612 0.159576 — -0.030388 0.241272 0.003072

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000625 0.000338 0.000155 0.000262 0.000286 0.000146 0.000496 0.000661 0.000319 0.000221 0.000170 0.000461
Bud Neck 0.004835 0.009436 0.004906 0.004748 0.008748 0.026967 0.002233 0.008346 0.008371 0.020100 0.004792 0.006544
Bud Site 0.023637 0.010609 0.006914 0.010987 0.006520 0.016137 0.002922 0.039934 0.008166 0.013971 0.006744 0.025434
Cell Periphery 0.000344 0.000119 0.000087 0.000130 0.000210 0.000181 0.000171 0.000183 0.000223 0.000157 0.000091 0.000156
Cytoplasm 0.620204* 0.639946* 0.440259* 0.565437* 0.595426* 0.540356* 0.325675* 0.559069* 0.597809* 0.579367* 0.435379* 0.562259*
Cytoplasmic Foci 0.136305 0.085312 0.004332 0.017226 0.120103 0.119915 0.008763 0.014909 0.121661 0.106360 0.004521 0.016069
Eisosomes 0.000069 0.000042 0.000015 0.000035 0.000069 0.000098 0.000056 0.000032 0.000069 0.000076 0.000017 0.000034
Endoplasmic Reticulum 0.005921 0.001941 0.001666 0.002164 0.001062 0.000736 0.007850 0.001363 0.001530 0.001208 0.001929 0.001764
Endosome 0.005984 0.005508 0.000900 0.001930 0.004295 0.009957 0.003091 0.005599 0.004458 0.008214 0.000994 0.003761
Golgi 0.001055 0.001257 0.000059 0.000708 0.000913 0.001546 0.000097 0.001828 0.000927 0.001433 0.000061 0.001267
Lipid Particles 0.003043 0.001779 0.000437 0.001027 0.002707 0.004348 0.001806 0.000821 0.002740 0.003342 0.000495 0.000924
Mitochondria 0.000802 0.001810 0.000806 0.001098 0.001418 0.005708 0.000457 0.002593 0.001359 0.004181 0.000791 0.001844
Mitotic Spindle 0.003847 0.006309 0.016867 0.012418 0.002209 0.005027 0.008397 0.035975 0.002366 0.005529 0.016507 0.024175
None 0.002698 0.006401 0.008975 0.005439 0.004164 0.006736 0.005667 0.006387 0.004023 0.006605 0.008834 0.005912
Nuclear Periphery 0.000461 0.000512 0.001638 0.001312 0.000633 0.000449 0.002239 0.001024 0.000617 0.000474 0.001664 0.001168
Nuclear Periphery Foci 0.002749 0.001114 0.000557 0.001247 0.000645 0.000232 0.003580 0.000766 0.000847 0.000578 0.000686 0.001007
Nucleolus 0.000564 0.001119 0.000421 0.000608 0.000835 0.001443 0.000408 0.000511 0.000809 0.001316 0.000420 0.000559
Nucleus 0.112238 0.178707* 0.478482* 0.284064* 0.214546* 0.195319* 0.495586* 0.233599* 0.204708* 0.188812* 0.479211* 0.258877*
Peroxisomes 0.001180 0.002781 0.000141 0.000505 0.001724 0.003899 0.000164 0.001013 0.001671 0.003461 0.000142 0.000759
Vacuole 0.071881 0.043996 0.032224 0.088445 0.033063 0.059356 0.130172 0.081884 0.036796 0.053340 0.036395 0.085170
Vacuole Periphery 0.001557 0.000962 0.000158 0.000210 0.000423 0.001445 0.000169 0.003504 0.000532 0.001256 0.000159 0.001854

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.30 3.62 2.99 0.72 -2.55 0.91 -2.83 -1.31 -3.73 2.05 0.69 0.54 -0.59 -3.13 -3.09
Bud Neck -4.02 -0.44 -0.99 3.82 -0.75 -11.21 7.37 0.74 11.26 -5.58 -10.54 4.34 2.00 12.19 -2.61
Bud Site 2.63 3.61 2.66 0.16 -2.71 -5.33 1.70 -7.30 -3.28 -8.45 -4.01 1.80 -5.62 -2.24 -7.54
Cell Periphery 5.68 6.40 5.72 0.30 -3.34 0.73 0.47 1.74 2.82 2.49 1.80 3.43 2.57 3.16 -4.12
Cytoplasm -0.98 12.83 7.80 17.96 -10.55 6.40 15.93 14.56 9.07 -9.91 2.76 29.59 18.07 17.84 -13.61
Cytoplasmic Foci 4.61 12.55 11.23 19.66 -14.59 -0.07 29.52 28.63 32.00 -4.80 3.56 33.98 30.34 37.10 -17.98
Eisosomes 2.88 5.46 2.47 -1.29 -13.32 -3.67 -1.25 4.76 9.65 4.89 -1.04 8.95 4.73 7.80 -14.50
Endoplasmic Reticulum 2.83 2.41 2.25 -3.39 -1.10 2.95 -6.96 -7.94 -11.32 6.14 1.79 -6.72 -5.29 -10.23 2.67
Endosome 0.82 7.81 6.08 6.68 -6.14 -8.05 1.38 1.51 8.19 0.26 -7.21 12.10 4.71 10.92 -6.47
Golgi -0.61 4.41 2.05 2.71 -1.27 -2.29 4.29 0.20 1.98 -2.46 -2.23 5.14 1.26 3.37 -2.85
Lipid Particles 2.00 4.02 2.72 1.83 -9.31 -3.56 0.80 7.18 7.88 4.69 -1.77 10.25 7.21 7.91 -10.67
Mitochondria -2.81 -0.73 -0.18 2.66 0.48 -4.69 3.19 -0.43 4.43 -3.26 -4.71 1.81 0.49 5.12 -1.54
Mitotic Spindle -0.83 -5.05 -3.31 -3.58 2.16 -2.40 -1.38 -8.41 -7.26 -2.61 -3.06 -8.20 -9.87 -8.02 -0.04
None -3.55 -6.61 -2.93 1.37 4.34 -2.80 -0.22 -2.82 0.70 -1.64 -3.51 -5.74 -2.71 1.53 3.79
Nuclear Periphery -0.45 -12.90 -10.27 -16.62 2.64 3.48 -8.16 -15.76 -19.23 3.42 2.94 -24.08 -20.19 -25.22 4.76
Nuclear Periphery Foci 3.48 4.56 0.53 -2.63 -3.65 5.36 -5.26 -3.62 -5.57 4.13 2.97 -0.22 -4.08 -5.23 -4.04
Nucleolus -2.25 0.88 -1.07 1.74 -2.72 -3.00 2.31 -1.24 0.50 -2.60 -2.81 2.66 -0.84 1.73 -3.42
Nucleus -5.66 -33.04 -15.62 -16.42 25.86 3.15 -7.69 -9.73 -13.08 5.17 2.85 -41.93 -14.94 -20.69 30.48
Peroxisomes -3.22 8.38 4.55 4.78 -2.94 -5.26 6.57 4.26 8.91 -3.99 -5.13 7.23 5.04 10.00 -4.87
Vacuole 3.07 2.33 -8.91 -19.82 -18.63 -9.49 -8.02 -22.21 -16.80 3.76 -7.73 -8.58 -30.18 -25.78 -20.01
Vacuole Periphery 1.49 4.18 4.12 3.50 -0.67 -4.60 5.92 -2.10 -0.21 -2.55 -4.32 8.09 -1.20 1.22 -2.65
Standard name
Human Ortholog
Description Xanthine-guanine phosphoribosyl transferase; required for xanthine utilization and for optimal utilization of guanine
Localization
Cell Percentages cytoplasm (72%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Xpt1

Xpt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Xpt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available