Standard name
Human Ortholog
Description RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 – 0 0.09 0.07 0.05 – 0.05 0 0 0 – 0 0.07 0.06 0 0.11 0 0 0 0 0 0
Endoplasmic Reticulum – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0
Mitochondria – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0.1 0.06 0.14 0 0 0 0 0 0
Nucleus – 0.92 – 0.75 0.74 0.72 0.77 – 0.67 0.7 0.64 0.67 – 0.75 0.67 0.61 0.67 0.43 0.46 0.74 0.36 0.43 0.43 0.39
Nuclear Periphery – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0.68 – 0.6 0.3 0.54 0.34 – 0.55 0.67 0.67 0.56 – 0.59 0.52 0.53 0.31 0.55 0.46 0.23 0.54 0.51 0.5 0.51
Peroxisomes – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 5 3
Bud Neck – 0 – 0 0 0 0 – 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 – 0 0 1 0 – 0 0 0 1 – 1 2 0 0 0 – – – – – –
Cell Periphery – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 – 2 10 7 11 – 9 3 3 6 – 7 15 6 3 6 0 0 0 0 0 2
Endoplasmic Reticulum – 0 – 0 0 0 0 – 0 1 0 0 – 0 0 2 0 0 0 0 0 0 0 0
Endosome – 0 – 0 1 0 2 – 0 0 0 0 – 2 1 0 0 1 0 0 0 1 0 0
Golgi – 0 – 0 0 0 0 – 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0
Mitochondria – 0 – 0 1 4 0 – 5 2 4 1 – 2 1 10 4 8 0 0 0 0 0 1
Nucleus – 23 – 41 80 72 156 – 124 134 107 119 – 117 149 62 45 24 6 52 6 55 102 69
Nuclear Periphery – 0 – 0 0 0 0 – 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 17 – 33 32 54 69 – 101 128 113 100 – 92 116 54 21 31 6 15 9 65 120 90
Peroxisomes – 1 – 0 0 0 0 – 0 0 0 1 – 1 3 0 1 0 0 0 0 0 0 0
SpindlePole – 1 – 0 1 0 2 – 1 0 0 0 – 1 7 0 0 1 0 0 0 0 2 1
Vac/Vac Membrane – 1 – 1 2 0 1 – 2 1 0 1 – 1 1 1 0 1 0 1 1 1 4 6
Unique Cell Count 25 55 108 100 203 185 191 168 177 157 222 101 67 56 14 70 17 128 239 178
Labelled Cell Count 43 77 127 138 241 242 269 227 231 224 295 136 74 72 14 70 17 128 239 178


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 24.9 – 18.8 19.5 11.0 17.9 – 15.2 15.1 14.3 15.3 – 17.5 23.6 21.5 23.5 23.7 20.2 22.3 21.9
Std Deviation (1e-4) – 6.9 – 5.6 9.1 3.9 4.8 – 6.9 4.5 3.8 5.7 – 7.1 11.4 9.9 9.3 12.4 5.9 10.6 7.9
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6393 13.7446 12.1116 12.1176 6.5842 10.8705 15.244 18.414 15.2703 15.1915 12.5399 13.9334 18.1633 18.1197 15.5534 15.8032 17.8465 16.5882
Actin 0.0215 0 0.0022 0 0.057 0.0018 0.0081 0 0.0098 0.0021 0.0002 0.0009 0.0018 0 0.0087 0 0 0.0031
Bud 0.0009 0 0.0002 0 0.001 0.0001 0.0018 0.0001 0.0009 0.0004 0.0001 0.0004 0.0002 0 0.0003 0.0001 0.0001 0.0001
Bud Neck 0.0005 0 0.0002 0.0001 0.0011 0.0002 0.0004 0 0.0002 0.0003 0.0002 0.0009 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002
Bud Periphery 0.0011 0 0.0003 0 0.0024 0.0001 0.002 0 0.0011 0.0006 0.0002 0.0005 0.0003 0 0.0004 0.0001 0.0003 0.0001
Bud Site 0.002 0 0.0005 0 0.0017 0.0001 0.0079 0.0001 0.0023 0.0013 0.0002 0.0003 0.0006 0 0.0008 0.0002 0.0002 0.0001
Cell Periphery 0.0003 0 0.0002 0 0.0006 0 0.0003 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0 0 0.0001 0.0003 0
Cytoplasm 0.0124 0 0.0004 0 0.0087 0.0022 0.0027 0.0001 0.001 0.0002 0.0087 0.0013 0.0017 0.0001 0.0002 0 0 0.0001
Cytoplasmic Foci 0.0232 0 0.0018 0 0.0127 0.0008 0.0332 0 0.0026 0.0022 0.0005 0.0055 0.0051 0 0.0026 0 0 0.0013
Eisosomes 0.0002 0 0 0 0.0006 0 0.0002 0 0.0001 0.0001 0 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0054 0 0.0013 0 0.0092 0.0015 0.0023 0 0.001 0.0002 0.0016 0.0009 0.0022 0 0.0002 0 0 0.0001
Endosome 0.0696 0 0.0022 0 0.0608 0.0083 0.02 0 0.0076 0.0009 0.0091 0.0174 0.0093 0 0.0013 0 0 0.0027
Golgi 0.0359 0 0.0004 0 0.0187 0.0016 0.0057 0 0.0028 0.0005 0.0001 0.0033 0.0008 0 0.0013 0 0 0.0028
Lipid Particles 0.0493 0 0.0003 0 0.0357 0.0024 0.0496 0 0.0121 0.0034 0.0001 0.0015 0.0021 0 0.0013 0 0.0002 0.0129
Mitochondria 0.0147 0.0001 0.0044 0.0002 0.0695 0.0011 0.0095 0.0004 0.0159 0.0135 0.0051 0.0017 0.0068 0.0005 0.0014 0.0008 0.0322 0.0011
None 0.0054 0 0.0003 0 0.0033 0.0003 0.0036 0 0.0008 0.0003 0.0007 0.0006 0.0015 0 0.0003 0 0 0.0001
Nuclear Periphery 0.1179 0.0002 0.0152 0.0001 0.0606 0.0263 0.029 0.0048 0.0156 0.0057 0.0178 0.0047 0.1537 0.0033 0.0047 0.0001 0.0001 0.0022
Nucleolus 0.2158 0.1544 0.188 0.1738 0.3192 0.462 0.1585 0.1344 0.1058 0.1843 0.6279 0.4877 0.2286 0.1838 0.1519 0.151 0.7758 0.4373
Nucleus 0.3625 0.845 0.7444 0.8253 0.2856 0.486 0.5816 0.8596 0.803 0.7677 0.3201 0.454 0.4707 0.8093 0.8116 0.8467 0.1892 0.5303
Peroxisomes 0.0116 0 0.0003 0 0.0088 0.0002 0.0103 0 0.0019 0.0004 0.0001 0.0004 0.0003 0 0.0011 0 0.0001 0.0024
Punctate Nuclear 0.0318 0.0001 0.0339 0.0001 0.0138 0.0029 0.0669 0.0001 0.0084 0.0136 0.0014 0.0154 0.0854 0.0025 0.0115 0.0004 0.0002 0.0026
Vacuole 0.0076 0 0.0021 0.0001 0.0191 0.0008 0.0027 0.0002 0.0036 0.0007 0.0036 0.0011 0.0045 0.0001 0.0001 0.0001 0.0003 0.0001
Vacuole Periphery 0.0104 0 0.0014 0.0001 0.01 0.0012 0.0038 0.0001 0.0036 0.0013 0.0022 0.0012 0.0241 0.0002 0.0002 0.0001 0.0007 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.6292 38.2316 26.951 53.0236 38.8151 29.9208 40.5888 46.3101 42.8994 56.0477
Translational Efficiency 1.9116 2.0073 2.5092 1.2978 1.6684 2.1021 2.156 1.6477 1.5881 1.4192

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘