Standard name
Description RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.21 0.09 0.08 0 0 0 0.05 0 0 0 0.11 0.08 0.17 0.14 0.27 0.19 0.27 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.18 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0 0.09 0.27 0.26 0.25 0.33 0.44 0.43 0.4 0 0 0 0.11 0.14 0.17 0 0 0 0 0 0
Nucleus 0.8 0.76 0.62 0.76 0.76 0.6 0.61 0.58 0.54 0.54 0.52 0.5 0.84 0.68 0.65 0.4 0.46 0.29 0.9 0.78 0.7 0.63 0.48 0.38
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Nucleolus 0.25 0.12 0.3 0.22 0.37 0.59 0.55 0.51 0.6 0.64 0.68 0.62 0.08 0.14 0.21 0.14 0.09 0.11 0 0 0.1 0.14 0.16 0.19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.13 0.1 0.12 0 0.09 0.1 0.16 0.21 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 1 2 3
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Cytoplasm 3 37 10 8 5 6 12 16 6 13 6 33 12 26 32 45 22 29 0 0 0 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 14 14 20 1 5 0 2 7 8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 1 1 0 1 1 0 1 0 0 0 2 3 4 2 2 1 0 0 0 0 3 3
Mitochondria 4 1 8 4 15 53 97 77 95 187 128 117 0 0 3 19 16 18 0 0 0 0 0 1
Nucleus 82 131 68 72 129 118 224 180 154 231 153 146 133 103 149 67 52 32 114 174 99 75 122 98
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 11 6
Nucleolus 26 21 33 21 63 115 202 158 169 272 201 180 12 21 49 23 10 12 2 8 13 17 41 48
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2
Vac/Vac Membrane 2 10 4 3 2 3 1 10 4 5 4 2 3 3 11 22 11 13 4 18 14 18 52 66
Unique Cell Count 103 173 109 95 170 196 367 309 284 428 296 290 159 152 230 167 114 109 128 223 143 120 256 257
Labelled Cell Count 119 200 124 109 214 296 537 441 431 708 492 479 163 156 248 192 127 127 128 223 143 120 256 257


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 128.0 154.6 140.5 125.6 121.9 105.0 107.2 100.8 101.3 93.4 89.1 93.6 158.2 159.3 149.5 189.7 169.1 180.9 140.9 153.4 163.1
Std Deviation (1e-4) 30.5 38.2 38.2 41.4 33.3 33.1 34.4 34.7 29.7 30.6 27.4 32.7 45.8 54.5 51.3 63.6 59.7 64.5 44.2 43.1 49.7
Intensity Change (Log2) -0.16 -0.2 -0.42 -0.39 -0.48 -0.47 -0.59 -0.66 -0.59 0.17 0.18 0.09 0.43 0.27 0.36 0.0 0.13 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -2.3 -2.3 -2.6 -1.5 0 0 0 0.6 -0.5 1.8 1.2 3.6 2.2 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.1 3.8 4.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.9 0.4 4.1 4.2 3.9 5.3 7.0 6.8 6.3 0 0 0 1.1 1.6 2.1
Nucleus 2.1 2.4 -0.4 -0.3 -0.8 -1.5 -1.6 -1.9 -2.1 3.9 0.9 0.4 -3.6 -2.5 -4.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.3 1.2 4.8 4.5 3.8 5.2 6.3 6.8 5.7 -4.9 -3.2 -1.8 -3.3 -4.1 -3.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.6 1.8 2.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npl3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available