Standard name
Human Ortholog
Description RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.21 0.09 0.08 0 0 0 0.05 0 0 0 0.11 0.08 0.17 0.14 0.27 0.19 0.27 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.18 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0 0.09 0.27 0.26 0.25 0.33 0.44 0.43 0.4 0 0 0 0.11 0.14 0.17 0 0 0 0 0 0
Nucleus 0.8 0.76 0.62 0.76 0.76 0.6 0.61 0.58 0.54 0.54 0.52 0.5 0.84 0.68 0.65 0.4 0.46 0.29 0.9 0.78 0.7 0.63 0.48 0.38
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Nucleolus 0.25 0.12 0.3 0.22 0.37 0.59 0.55 0.51 0.6 0.64 0.68 0.62 0.08 0.14 0.21 0.14 0.09 0.11 0 0 0.1 0.14 0.16 0.19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.13 0.1 0.12 0 0.09 0.1 0.16 0.21 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 1 2 3
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Cytoplasm 3 37 10 8 5 6 12 16 6 13 6 33 12 26 32 45 22 29 0 0 0 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 14 14 20 1 5 0 2 7 8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 1 1 0 1 1 0 1 0 0 0 2 3 4 2 2 1 0 0 0 0 3 3
Mitochondria 4 1 8 4 15 53 97 77 95 187 128 117 0 0 3 19 16 18 0 0 0 0 0 1
Nucleus 82 131 68 72 129 118 224 180 154 231 153 146 133 103 149 67 52 32 114 174 99 75 122 98
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 11 6
Nucleolus 26 21 33 21 63 115 202 158 169 272 201 180 12 21 49 23 10 12 2 8 13 17 41 48
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2
Vac/Vac Membrane 2 10 4 3 2 3 1 10 4 5 4 2 3 3 11 22 11 13 4 18 14 18 52 66
Unique Cell Count 103 173 109 95 170 196 367 309 284 428 296 290 159 152 230 167 114 109 128 223 143 120 256 257
Labelled Cell Count 119 200 124 109 214 296 537 441 431 708 492 479 163 156 248 192 127 127 128 223 143 120 256 257


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 128.0 154.6 140.5 125.6 121.9 105.0 107.2 100.8 101.3 93.4 89.1 93.6 158.2 159.3 149.5 189.7 169.1 180.9 140.9 153.4 163.1
Std Deviation (1e-4) 30.5 38.2 38.2 41.4 33.3 33.1 34.4 34.7 29.7 30.6 27.4 32.7 45.8 54.5 51.3 63.6 59.7 64.5 44.2 43.1 49.7
Intensity Change (Log2) – – – -0.16 -0.2 -0.42 -0.39 -0.48 -0.47 -0.59 -0.66 -0.59 0.17 0.18 0.09 0.43 0.27 0.36 0.0 0.13 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -2.3 -2.3 -2.6 -1.5 0 0 0 0.6 -0.5 1.8 1.2 3.6 2.2 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.1 3.8 4.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.9 0.4 4.1 4.2 3.9 5.3 7.0 6.8 6.3 0 0 0 1.1 1.6 2.1
Nucleus 2.1 2.4 -0.4 -0.3 -0.8 -1.5 -1.6 -1.9 -2.1 3.9 0.9 0.4 -3.6 -2.5 -4.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.3 1.2 4.8 4.5 3.8 5.2 6.3 6.8 5.7 -4.9 -3.2 -1.8 -3.3 -4.1 -3.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.6 1.8 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 93.1025 148.4228 124.9524 138.9225 46.6213 124.0974 129.765 193.5742 176.1996 167.7305 156.3638 181.7743 179.3778 192.2623 185.4258 176.6317 134.822 180.4142
Actin 0.0138 0 0.0131 0 0.0085 0 0.0074 0 0.0095 0.0024 0.0118 0 0.0126 0.0002 0 0 0.0119 0.0002
Bud 0.0005 0 0.0006 0 0.001 0 0.0013 0 0.0004 0.0001 0.0004 0.0001 0.0002 0 0.0001 0 0.0002 0.0001
Bud Neck 0.0004 0 0.0002 0 0.0018 0.0001 0.004 0 0.0002 0.0002 0.0032 0.0001 0.0005 0 0 0 0.0001 0.0001
Bud Periphery 0.0007 0 0.0006 0 0.0018 0.0001 0.0007 0 0.0005 0.0001 0.0006 0 0.0002 0 0.0001 0 0.0001 0
Bud Site 0.004 0 0.0029 0 0.0017 0 0.0039 0 0.0022 0.0001 0.0025 0 0.0008 0 0 0 0.0003 0.0004
Cell Periphery 0.0004 0 0.0001 0 0.0022 0.0002 0.0029 0 0.0003 0 0.0002 0.0001 0.0002 0 0.0004 0 0.0005 0.0004
Cytoplasm 0.0075 0.0001 0.0016 0.0002 0.0371 0.0004 0.0137 0.0001 0.0029 0.0002 0.0081 0.0001 0.0034 0.0004 0.0001 0.0001 0.0054 0.0043
Cytoplasmic Foci 0.027 0 0.004 0 0.0108 0.0003 0.0481 0 0.0155 0.0091 0.0097 0 0.0063 0 0.0002 0 0.0153 0.0105
Eisosomes 0.0007 0 0.0004 0 0.0003 0 0.0007 0 0.0001 0 0.0001 0 0.0002 0 0 0 0.0002 0.0003
Endoplasmic Reticulum 0.0026 0 0.0012 0 0.0132 0 0.0024 0 0.0002 0.0002 0.0283 0 0.0022 0.0002 0 0 0.0068 0.0002
Endosome 0.0112 0 0.0033 0 0.0953 0.0001 0.0161 0 0.0047 0.0119 0.0278 0 0.008 0.0004 0 0 0.04 0.0004
Golgi 0.004 0 0.0028 0 0.0114 0 0.0033 0 0.0013 0.0086 0.0055 0 0.0036 0 0 0 0.0049 0.0002
Lipid Particles 0.0128 0 0.0022 0 0.0089 0.0001 0.0129 0 0.0012 0.0026 0.0092 0 0.0053 0 0.0002 0 0.0097 0.0038
Mitochondria 0.0083 0.0007 0.0058 0.0005 0.0378 0.0009 0.0179 0.0006 0.0063 0.0082 0.005 0.0011 0.0109 0.0015 0.0049 0.0004 0.0101 0.0013
None 0.0126 0 0.0081 0 0.0389 0.0002 0.0163 0 0.0012 0.0001 0.0035 0 0.0033 0.0001 0.0001 0 0.0076 0.003
Nuclear Periphery 0.0142 0.0021 0.0103 0.0005 0.1817 0.0086 0.0193 0.0004 0.0013 0.0088 0.0216 0.0003 0.0032 0.0015 0.0001 0.0003 0.0223 0.0008
Nucleolus 0.0576 0.0162 0.0358 0.0082 0.0124 0.0752 0.0906 0.0332 0.0503 0.0166 0.0673 0.052 0.0268 0.0311 0.0369 0.0331 0.1388 0.0479
Nucleus 0.7916 0.9807 0.9023 0.9906 0.4252 0.9059 0.6967 0.9655 0.8976 0.9276 0.782 0.9456 0.9074 0.9637 0.9543 0.9659 0.7079 0.9226
Peroxisomes 0.0073 0 0.0007 0 0.0013 0 0.0036 0 0.0021 0.0016 0.0035 0 0.0014 0 0.0001 0 0.0014 0.0002
Punctate Nuclear 0.0173 0 0.0023 0 0.0435 0.0004 0.0207 0 0.0005 0.0003 0.0064 0 0.0017 0.0001 0 0 0.0085 0.0017
Vacuole 0.0024 0 0.0005 0 0.0351 0.0023 0.0104 0 0.0009 0.0003 0.0019 0.0002 0.0009 0.0002 0.0009 0 0.0025 0.0009
Vacuole Periphery 0.0028 0.0002 0.0011 0.0001 0.0301 0.0052 0.0069 0.0002 0.0009 0.0008 0.0016 0.0004 0.0009 0.0003 0.0015 0.0001 0.0054 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 201.6261 248.7728 309.8225 275.298 260.8425 270.1623 279.9717 269.7912 288.431 220.75
Translational Efficiency 3.1514 2.8293 2.4391 2.7329 2.914 2.6212 2.3499 2.6579 2.4705 2.5164

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (28%), cytoplasm (1%), mixed (70%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Npl3

Npl3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npl3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available