Standard name
Human Ortholog
Description Transmembrane protein; subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0.07 0 0 0.05 0.05 0.06 0.05 0 0.09 0.1 0.15 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.07 0.08 0.07 0.05 0.16 0.12 0.14 0.16 0.27 0 0 0 0 0 0.05
Cytoplasm 0.17 0.14 0.2 0.1 0.14 0.19 0.1 0.16 0.16 0.16 0.16 0.15 0.16 0.16 0.21 0.34 0.26 0.24 0 0 0 0 0 0
Endoplasmic Reticulum 0.23 0.75 0.56 0.65 0.38 0.28 0.27 0.41 0.43 0.36 0.41 0.45 0.8 0.77 0.69 0.05 0 0 0.9 0.81 0.75 0.76 0.8 0.77
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.09 0 0 0 0 0 0
Mitochondria 0.22 0 0.24 0.36 0.29 0.52 0.6 0.4 0.45 0.55 0.51 0.54 0 0 0.05 0.08 0.07 0.05 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0.1 0.05 0.05 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.43 0.14 0.11 0.05 0.32 0.2 0.21 0.12 0.13 0.13 0.1 0.08 0 0 0.06 0.35 0.33 0.28 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 10 8 4 5 7 6 20 9 12 9 9 5 10 5 26 18 22 2 6 5 1 5 8
Bud 0 0 2 2 5 8 16 7 6 16 6 3 0 0 0 2 0 2 1 5 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 1 0
Bud Site 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0
Cell Periphery 3 1 1 1 3 8 6 12 12 25 13 11 4 31 16 40 30 40 0 0 0 1 4 9
Cytoplasm 18 36 34 13 27 46 27 45 35 58 27 25 13 31 27 100 49 36 0 10 5 3 2 4
Endoplasmic Reticulum 24 191 94 84 72 70 69 117 97 126 67 75 65 146 90 14 1 4 136 242 141 55 113 129
Endosome 6 1 1 0 5 2 2 1 0 0 0 0 0 0 0 11 2 1 0 4 2 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11 39 13 0 5 1 0 1 1
Mitochondria 23 8 40 46 55 129 156 112 100 195 83 89 2 5 7 22 13 8 4 4 11 2 2 5
Nucleus 0 1 2 0 1 3 2 3 4 4 0 2 0 1 0 2 2 2 0 0 0 1 1 0
Nuclear Periphery 1 9 9 13 9 13 16 15 15 15 7 7 2 5 2 1 3 0 2 4 6 0 1 0
Nucleolus 1 0 1 0 1 0 1 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0
Vac/Vac Membrane 45 35 19 7 61 49 54 33 30 46 16 14 0 3 8 104 63 41 2 9 7 2 4 4
Unique Cell Count 104 255 169 129 191 247 260 283 223 353 164 166 81 190 131 293 189 148 152 299 188 72 143 169
Labelled Cell Count 122 292 211 170 244 335 356 366 309 500 228 237 91 232 155 336 222 169 152 299 188 72 143 169


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 9.1 7.1 6.8 7.1 6.5 5.8 6.9 6.8 6.3 6.4 6.6 9.0 9.2 8.7 9.6 11.9 12.0 7.7 8.7 9.7
Std Deviation (1e-4) 1.9 2.2 1.9 1.6 1.5 1.6 1.4 1.6 1.5 1.4 1.3 1.7 2.0 2.2 2.4 2.6 3.0 3.0 1.5 2.1 1.8
Intensity Change (Log2) -0.06 -0.0 -0.14 -0.3 -0.06 -0.08 -0.17 -0.17 -0.11 0.34 0.37 0.28 0.43 0.74 0.75 0.12 0.28 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.7 -1.1 -1.0 -1.4 1.0 -0.3 -0.7 0.3 0.3 0 0.2 -0.4 1.6 1.7 3.1
Bud 0 0 0 2.5 0 0 2.0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.6 3.2 3.3 3.0 0 5.2 4.3 4.8 5.1 7.0
Cytoplasm -2.4 -1.5 -0.4 -2.8 -1.1 -1.1 -1.0 -0.9 -1.2 -0.8 -0.9 0.1 3.2 1.3 0.9
Endoplasmic Reticulum 1.7 -3.4 -5.6 -6.1 -2.9 -2.4 -4.3 -2.7 -1.9 3.8 4.3 2.3 -12.4 -11.8 -10.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 6.3 3.9
Mitochondria 2.3 1.1 5.8 7.4 3.5 4.3 6.8 5.1 5.6 -4.2 -6.0 -4.3 -4.9 -4.5 -4.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1.6 -0.3 0 0.4 0 0.6 -0.5 -0.5 -0.5 0 -1.3 0 0 -2.0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.8 4.7 2.3 2.6 0.1 0.7 0.6 -0.4 -0.9 -3.1 -3.8 -1.5 5.7 5.0 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7751 5.174 5.0691 5.8209 9.1021 5.151 3.6614 4.6706 4.7568 4.1224 3.1993 5.0494 5.4307 5.4348 5.609 5.4337 5.5714 5.8577
Actin 0 0.0012 0.0011 0.0006 0.0023 0.0046 0.002 0.0021 0.029 0.0123 0.0474 0.0035 0.0063 0.0117 0.0162 0.0067 0.0029 0.0132
Bud 0 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0003 0 0.0003 0.0004 0.0003 0.0002 0.0004 0
Bud Neck 0 0.0006 0.0092 0.0001 0.0002 0.003 0.0002 0.0001 0.0005 0.002 0.0007 0.0004 0.0006 0.0014 0.0004 0.0003 0.0007 0.0004
Bud Periphery 0 0.0001 0.0003 0 0.0002 0.0001 0.0001 0.0007 0.0004 0.0002 0.0004 0.0001 0.0005 0.0015 0.0007 0.0003 0.0006 0.0001
Bud Site 0 0.008 0.0004 0 0.0002 0.0002 0.0003 0.0017 0.0034 0.0006 0.0016 0 0.0024 0.0171 0.0009 0.0004 0.0023 0
Cell Periphery 0.0001 0.0159 0.0055 0.0003 0.0004 0.0025 0.0005 0.004 0.0024 0.0006 0.0005 0.0003 0.0008 0.0032 0.0012 0.0007 0.0004 0.0005
Cytoplasm 0.0012 0.2391 0.1763 0.033 0.0138 0.0836 0.0556 0.2001 0.0415 0.165 0.1312 0.0629 0.1263 0.1249 0.0792 0.1127 0.0911 0.0801
Cytoplasmic Foci 0.0001 0.0169 0.0032 0.0008 0.0019 0.0038 0.0241 0.0058 0.0069 0.0344 0.0307 0.0061 0.0277 0.0108 0.0053 0.0033 0.0332 0.0044
Eisosomes 0 0 0.0003 0 0.0001 0 0.0003 0.0023 0.0003 0.0001 0.0008 0.0001 0.0001 0.0002 0.0002 0.0001 0 0.0001
Endoplasmic Reticulum 0.927 0.5912 0.6696 0.9402 0.9426 0.8265 0.6739 0.612 0.7589 0.5579 0.5077 0.7731 0.6654 0.725 0.8084 0.7739 0.6019 0.82
Endosome 0.0035 0.087 0.0041 0.0047 0.0115 0.0061 0.0362 0.0059 0.0381 0.0459 0.0406 0.0172 0.0408 0.0114 0.0093 0.0339 0.0895 0.0047
Golgi 0.0001 0.0047 0.0011 0.0003 0.0025 0.0017 0.036 0.0012 0.0195 0.0646 0.0502 0.0137 0.012 0.0025 0.0042 0.0111 0.0225 0.0005
Lipid Particles 0.0014 0.0157 0.006 0.0008 0.0019 0.0071 0.0317 0.0062 0.0065 0.0075 0.0536 0.0066 0.0137 0.0099 0.0066 0.0033 0.0324 0.0043
Mitochondria 0.0001 0.0002 0.0002 0.0001 0.0009 0.0004 0.0033 0.0005 0.0177 0.0094 0.0802 0.0026 0.0042 0.001 0.0013 0.0094 0.0045 0.0001
None 0.0004 0.0011 0.1103 0.0013 0.0005 0.0014 0.0869 0.142 0.0474 0.0668 0.0193 0.0802 0.0233 0.0139 0.0317 0.0213 0.032 0.0416
Nuclear Periphery 0.0606 0.0106 0.0076 0.0132 0.0169 0.0447 0.0129 0.0081 0.0125 0.0077 0.0082 0.0262 0.0404 0.0405 0.0256 0.0152 0.0457 0.0247
Nucleolus 0 0 0 0 0 0.0001 0.0004 0.0002 0.0002 0.0001 0.0003 0 0.0005 0.0002 0.0002 0.0001 0.0004 0
Nucleus 0.0005 0.0012 0.0013 0.0013 0.0009 0.0017 0.0009 0.001 0.001 0.001 0.0008 0.0012 0.0091 0.0083 0.0023 0.0012 0.0064 0.0016
Peroxisomes 0 0.0002 0.0001 0 0.0001 0.0001 0.0111 0.0001 0.0063 0.0191 0.0193 0.0004 0.0109 0.0034 0.0008 0.0001 0.0023 0.0001
Punctate Nuclear 0.0001 0.0012 0.0003 0.0003 0.0002 0.0004 0.0206 0.0006 0.0006 0.001 0.0017 0.0004 0.0027 0.0032 0.0011 0.0003 0.018 0.0003
Vacuole 0.0026 0.0046 0.0027 0.0025 0.0022 0.0102 0.0023 0.0048 0.0047 0.0028 0.0034 0.0037 0.0068 0.0067 0.0027 0.0038 0.0104 0.0027
Vacuole Periphery 0.0022 0.0005 0.0002 0.0004 0.0007 0.0015 0.0007 0.0004 0.0016 0.0007 0.0011 0.0013 0.005 0.0025 0.0014 0.0017 0.0024 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.8741 17.2078 19.6094 34.5569 24.9577 20.613 33.8655 35.8581 29.7731 43.5309
Translational Efficiency 1.4649 1.8815 1.6278 0.7706 1.201 1.7209 1.0876 0.6825 0.9684 0.7988

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2466 1319 1728 1679 587 2238 81 82 3053 3557 1809 1761

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 715.43 749.83 916.68 917.55 751.75 820.45 998.85 995.19 722.41 794.26 920.36 921.17
Standard Deviation 81.24 113.13 146.24 109.88 84.01 111.06 102.22 121.75 83.02 116.92 145.55 111.66
Intensity Change Log 2 0.067753 0.357608 0.358976 0.126162 0.410015 0.404719 0.097976 0.384698 0.382595

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000312 0.002156 0.001808 0.016130 0.001488 0.000634 0.001763 0.014315 0.000538 0.001198 0.001806 0.016046
Bud Neck 0.042805 0.024454 0.014499 0.027788 0.024508 0.011000 0.028297 0.018037 0.039287 0.015989 0.015117 0.027334
Bud Site 0.022624 0.075401 0.053376 0.134412* 0.036622 0.030758 0.053892 0.138499* 0.025316 0.047313 0.053399 0.134602*
Cell Periphery 0.000433 0.000326 0.000146 0.000292 0.000616 0.000206 0.000203 0.000254 0.000468 0.000251 0.000149 0.000290
Cytoplasm 0.112914* 0.043361 0.060905 0.263707* 0.045160 0.031366 0.120409* 0.191589* 0.099887 0.035814 0.063570 0.260349*
Cytoplasmic Foci 0.232265* 0.119743 0.142985 0.132182 0.188032* 0.128377 0.161444 0.184282* 0.223760* 0.125175 0.143812 0.134608
Eisosomes 0.000214 0.000121 0.000064 0.000185 0.000223 0.000089 0.000172 0.000170 0.000216 0.000101 0.000069 0.000185
Endoplasmic Reticulum 0.004186 0.001645 0.005628 0.004166 0.001690 0.000661 0.005024 0.002827 0.003706 0.001026 0.005601 0.004104
Endosome 0.085354 0.098947 0.081786 0.023152 0.100802 0.096661 0.098226 0.040691 0.088324 0.097509 0.082522 0.023968
Golgi 0.054499 0.093339 0.067464 0.022632 0.102898 0.150650* 0.006251 0.013062 0.063805 0.129398* 0.064723 0.022186
Lipid Particles 0.024502 0.004665 0.003229 0.002981 0.007680 0.002366 0.008567 0.002834 0.021268 0.003219 0.003468 0.002974
Mitochondria 0.248737* 0.344136* 0.134895* 0.040255 0.345103* 0.429900* 0.023684 0.002612 0.267265* 0.398098* 0.129916* 0.038502
Mitotic Spindle 0.004165 0.022877 0.127027* 0.056636 0.012055 0.020647 0.096381 0.082647 0.005682 0.021474 0.125655* 0.057847
None 0.002074 0.001106 0.001089 0.000651 0.000557 0.000670 0.001214 0.000328 0.001783 0.000831 0.001095 0.000636
Nuclear Periphery 0.000733 0.000472 0.003173 0.000817 0.000252 0.000184 0.001445 0.000461 0.000640 0.000291 0.003095 0.000801
Nuclear Periphery Foci 0.000505 0.000768 0.002679 0.001343 0.000306 0.000191 0.002639 0.001478 0.000467 0.000405 0.002678 0.001349
Nucleolus 0.002681 0.002711 0.003184 0.000587 0.001412 0.000598 0.005630 0.000406 0.002437 0.001381 0.003293 0.000578
Nucleus 0.028054 0.010325 0.025866 0.051454 0.009175 0.002372 0.058910 0.026629 0.024424 0.005321 0.027345 0.050298
Peroxisomes 0.017994 0.031757 0.013646 0.058480 0.039830 0.018754 0.033156 0.072125 0.022193 0.023576 0.014520 0.059115
Vacuole 0.087109 0.081101 0.194460 0.155983 0.045776 0.043697 0.274877* 0.205533* 0.079162 0.057567 0.198061 0.158291
Vacuole Periphery 0.027839 0.040589 0.062088 0.006168 0.035817 0.030219 0.017817 0.001222 0.029373 0.034064 0.060106 0.005938

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.98 -10.82 -10.22 -8.39 -8.93 3.21 -1.19 -1.54 -1.69 -1.44 -4.27 -9.12 -10.17 -9.57 -9.03
Bud Neck 6.44 12.24 7.06 -0.50 -6.35 4.94 -0.60 2.12 -1.38 2.08 11.99 11.94 6.58 -5.89 -5.91
Bud Site -13.68 -10.89 -19.89 -6.11 -11.21 1.52 -1.58 -3.17 -3.50 -2.21 -10.45 -10.22 -19.60 -13.75 -11.43
Cell Periphery 0.88 2.36 1.42 2.43 -5.81 3.25 3.24 2.96 -0.36 -0.33 2.12 3.15 2.06 -0.29 -5.81
Cytoplasm 15.10 10.53 -22.24 -33.41 -30.17 3.15 -4.62 -5.22 -5.79 -1.65 18.70 7.79 -24.84 -37.05 -29.73
Cytoplasmic Foci 20.38 17.19 22.22 1.21 5.38 7.61 2.74 2.10 -0.92 -0.17 22.98 16.88 21.90 2.38 5.19
Eisosomes 7.68 13.75 3.12 -3.90 -9.63 5.88 1.16 1.73 -1.98 0.83 11.41 14.65 3.51 -6.33 -9.34
Endoplasmic Reticulum 6.17 -3.07 -0.58 -7.87 2.72 4.20 -3.39 -2.47 -3.70 1.05 8.19 -4.38 -1.68 -10.40 2.84
Endosome -3.09 1.46 24.07 21.51 22.65* 0.65 1.32 7.98 8.88 4.41 -2.91 2.37 26.97 31.97 23.06
Golgi -8.37 -3.06 14.68 17.65 15.37 -6.59 15.67 13.76 28.74 -1.01 -18.16 0.35 18.71 34.72 15.25
Lipid Particles 12.60 15.62 15.86 1.95 0.64 5.33 -0.75 3.54 -1.82 3.62 15.65 15.70 16.21 0.36 1.28
Mitochondria -9.00 15.94 35.65* 34.31* 18.78* -5.83 21.91 26.33 60.65* 2.16 -17.20 20.24 41.62* 61.46* 18.78*
Mitotic Spindle -9.18 -23.26* -13.40 -7.05 12.17 -4.05 -3.11 -2.61 -2.23 0.40 -12.31 -23.17 -13.24 -8.33 11.92
None 3.92 4.47 6.48 2.25 2.88 -0.54 -1.25 0.71 1.04 1.46 4.92 3.69 6.22 0.85 3.12
Nuclear Periphery 1.35 -5.62 -1.90 -2.60 4.31 0.84 -4.63 -2.63 -2.92 2.09 3.27 -5.95 -2.39 -4.06 4.37
Nuclear Periphery Foci -0.92 -7.18 -3.51 -1.84 2.93 1.55 -3.88 -4.48 -4.68 1.18 0.47 -7.77 -4.00 -3.94 3.04
Nucleolus -0.40 -1.13 6.09 3.90 6.96 1.89 -1.61 2.20 1.07 2.21 2.80 -1.83 6.33 3.42 7.31
Nucleus 8.06 -0.14 -13.71 -19.19 -12.31 4.18 -4.51 -3.45 -4.26 1.72 13.43 -2.35 -15.34 -23.51 -11.61
Peroxisomes -5.54 3.29 -9.87 -3.92 -11.25 5.34 0.82 -1.01 -2.59 -1.36 -0.95 5.40 -8.52 -7.83 -11.24
Vacuole 0.95 -19.04 -20.00 -19.64 -2.20 0.45 -10.19 -8.27 -8.36 -0.38 6.89 -22.25 -22.76 -27.16 -2.24
Vacuole Periphery -5.38 -12.37 17.43 16.74 22.78* 1.61 4.87 10.98 23.15 4.04 -3.00 -11.43 19.43 23.46 22.88*