Standard name
Description Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.1 0.11 0.16 0.09 – 0.16 0.09 0.16 0.12 0 0.16 0.26 0.11 0.1 0.13 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0 – 0 0 0.16 0.15 – 0.38 0.29 0.6 0.4 0.06 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0.91 0.93 – 0.87 0.85 0.7 0.81 – 0.66 0.74 0.52 0.72 0.94 0.81 0.7 0.78 0.8 0.73 0.84 0.89 0.81 0.75 0.67 0.55
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 – 0 0 0.05 0.05 – 0 0 0 0.05 0.06 0 0 0.09 0.12 0.15 0.05 0.06 0.11 0.14 0.17 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 1 3 2 1 0 0 1 0 0 1 0 0 0 0 0 0
Bud Neck 0 0 – 0 1 1 3 – 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 5 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 3 – 13 25 28 30 – 27 23 14 5 0 23 46 18 16 25 0 0 0 1 2 4
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 1 0 0 1 0 6 2 6 0 0 0 0 1 0
Endosome 0 0 – 0 3 4 3 – 0 2 0 0 0 0 1 0 3 6 1 0 0 0 2 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 3 2 1 0 1 0 0 0 1
Mitochondria 12 2 – 1 0 27 51 – 62 72 52 16 1 1 2 3 4 6 1 0 0 0 4 2
Nucleus 40 100 – 111 192 120 276 – 109 184 45 29 16 113 126 132 128 136 51 102 15 13 82 82
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 – 0 0 0 0 – 1 0 3 1 1 1 1 1 1 2 0 0 0 0 2 6
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 2 7 5 13 – 2 7 5 0 0 2 2 0 1 0 0 0 0 0 1 3
Vac/Vac Membrane 1 9 – 4 7 9 16 – 3 11 0 2 1 6 8 16 20 27 2 7 2 2 20 42
Unique Cell Count 44 108 128 226 171 339 164 247 86 40 17 140 180 169 161 186 61 116 19 18 124 150
Labelled Cell Count 55 114 131 235 194 393 205 308 124 55 19 147 187 179 178 210 61 116 19 18 124 150


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.6 – 6.3 6.0 5.2 4.9 – 4.6 4.6 4.4 4.2 5.2 6.5 6.6 8.3 8.0 7.5 – 5.8 6.2
Std Deviation (1e-4) 0.5 0.8 – 1.7 1.5 1.7 1.4 – 1.8 1.3 1.9 1.5 0.8 1.3 2.2 1.8 1.7 1.6 – 1.6 2.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –