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Description | Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 3 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | 0 | 1 | 1 | 3 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | – | 13 | 25 | 28 | 30 | – | 27 | 23 | 14 | 5 | 0 | 23 | 46 | 18 | 16 | 25 | 0 | 0 | 0 | 1 | 2 | 4 |
Endoplasmic Reticulum | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 6 | 2 | 6 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 0 | 0 | – | 0 | 3 | 4 | 3 | – | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 6 | 1 | 0 | 0 | 0 | 2 | 0 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 12 | 2 | – | 1 | 0 | 27 | 51 | – | 62 | 72 | 52 | 16 | 1 | 1 | 2 | 3 | 4 | 6 | 1 | 0 | 0 | 0 | 4 | 2 |
Nucleus | 40 | 100 | – | 111 | 192 | 120 | 276 | – | 109 | 184 | 45 | 29 | 16 | 113 | 126 | 132 | 128 | 136 | 51 | 102 | 15 | 13 | 82 | 82 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 6 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 2 | 7 | 5 | 13 | – | 2 | 7 | 5 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 1 | 9 | – | 4 | 7 | 9 | 16 | – | 3 | 11 | 0 | 2 | 1 | 6 | 8 | 16 | 20 | 27 | 2 | 7 | 2 | 2 | 20 | 42 |
Unique Cell Count | 44 | 108 | 128 | 226 | 171 | 339 | 164 | 247 | 86 | 40 | 17 | 140 | 180 | 169 | 161 | 186 | 61 | 116 | 19 | 18 | 124 | 150 | ||
Labelled Cell Count | 55 | 114 | 131 | 235 | 194 | 393 | 205 | 308 | 124 | 55 | 19 | 147 | 187 | 179 | 178 | 210 | 61 | 116 | 19 | 18 | 124 | 150 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.6 | – | 6.3 | 6.0 | 5.2 | 4.9 | – | 4.6 | 4.6 | 4.4 | 4.2 | 5.2 | 6.5 | 6.6 | 8.3 | 8.0 | 7.5 | – | 5.8 | 6.2 |
Std Deviation (1e-4) | 0.5 | 0.8 | – | 1.7 | 1.5 | 1.7 | 1.4 | – | 1.8 | 1.3 | 1.9 | 1.5 | 0.8 | 1.3 | 2.2 | 1.8 | 1.7 | 1.6 | – | 1.6 | 2.0 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |