Standard name
Human Ortholog
Description Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.1 0.11 0.16 0.09 – 0.16 0.09 0.16 0.12 0 0.16 0.26 0.11 0.1 0.13 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0 – 0 0 0.16 0.15 – 0.38 0.29 0.6 0.4 0.06 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0.91 0.93 – 0.87 0.85 0.7 0.81 – 0.66 0.74 0.52 0.72 0.94 0.81 0.7 0.78 0.8 0.73 0.84 0.89 0.81 0.75 0.67 0.55
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 – 0 0 0.05 0.05 – 0 0 0 0.05 0.06 0 0 0.09 0.12 0.15 0.05 0.06 0.11 0.14 0.17 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 1 3 2 1 0 0 1 0 0 1 0 0 0 0 0 0
Bud Neck 0 0 – 0 1 1 3 – 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 5 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 3 – 13 25 28 30 – 27 23 14 5 0 23 46 18 16 25 0 0 0 1 2 4
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 1 0 0 1 0 6 2 6 0 0 0 0 1 0
Endosome 0 0 – 0 3 4 3 – 0 2 0 0 0 0 1 0 3 6 1 0 0 0 2 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 3 2 1 0 1 0 0 0 1
Mitochondria 12 2 – 1 0 27 51 – 62 72 52 16 1 1 2 3 4 6 1 0 0 0 4 2
Nucleus 40 100 – 111 192 120 276 – 109 184 45 29 16 113 126 132 128 136 51 102 15 13 82 82
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 – 0 0 0 0 – 1 0 3 1 1 1 1 1 1 2 0 0 0 0 2 6
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 2 7 5 13 – 2 7 5 0 0 2 2 0 1 0 0 0 0 0 1 3
Vac/Vac Membrane 1 9 – 4 7 9 16 – 3 11 0 2 1 6 8 16 20 27 2 7 2 2 20 42
Unique Cell Count 44 108 128 226 171 339 164 247 86 40 17 140 180 169 161 186 61 116 19 18 124 150
Labelled Cell Count 55 114 131 235 194 393 205 308 124 55 19 147 187 179 178 210 61 116 19 18 124 150


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.6 – 6.3 6.0 5.2 4.9 – 4.6 4.6 4.4 4.2 5.2 6.5 6.6 8.3 8.0 7.5 – 5.8 6.2
Std Deviation (1e-4) 0.5 0.8 – 1.7 1.5 1.7 1.4 – 1.8 1.3 1.9 1.5 0.8 1.3 2.2 1.8 1.7 1.6 – 1.6 2.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4589 1.4148 1.624 1.1988 1.8359 1.2749 0.9824 1.1786 1.271 0.6053 0.8184 0.9033 -1.1126 -0.8227 -0.6157 -0.8067 -0.9176 -0.758
Actin 0.0072 0.0002 0.0026 0.0065 0.0071 0.0003 0.0603 0.0002 0.001 0.0005 0.0178 0.0015 0.0309 0.0001 0.0282 0.0613 0 0.0001
Bud 0.0006 0.0001 0.0009 0.0002 0.0005 0.0002 0.0011 0.0001 0.0005 0.0002 0.0005 0.0001 0.0004 0 0.0002 0.0001 0 0.0002
Bud Neck 0.0042 0.0002 0.0002 0.0006 0.0055 0.0012 0.001 0.0003 0.0063 0.0001 0.0015 0.0051 0.0046 0.0002 0.0009 0.0004 0.0011 0.0019
Bud Periphery 0.0015 0.0001 0.0004 0.0007 0.0009 0.0002 0.0041 0.0001 0.0011 0.0003 0.0011 0.0004 0.0007 0 0.0002 0.0002 0 0.0002
Bud Site 0.0046 0.0002 0.0011 0.0004 0.002 0.0002 0.004 0.0007 0.0187 0.0002 0.001 0.0003 0.0027 0.0001 0.0138 0.0006 0 0.0001
Cell Periphery 0.0006 0 0.0001 0.0002 0.0002 0.0001 0.0008 0 0.0009 0 0.0004 0.0001 0.0004 0 0.0001 0.0001 0 0
Cytoplasm 0.0107 0.0036 0.0026 0.0008 0.0025 0.0016 0.0047 0.0094 0.0008 0.0002 0.0008 0.0007 0.002 0.0035 0.003 0.0012 0.0013 0.019
Cytoplasmic Foci 0.0112 0.0011 0.0078 0.0107 0.0137 0.0008 0.0313 0.0003 0.0054 0 0.0186 0.0026 0.0157 0.001 0.0156 0.0084 0 0.0002
Eisosomes 0.0002 0 0.0004 0.0001 0.0001 0.0001 0.0029 0 0 0 0.0001 0 0.0008 0 0.0001 0.0006 0 0
Endoplasmic Reticulum 0.0018 0.0003 0.0006 0.0009 0.0006 0.0006 0.008 0.0007 0.0006 0.0001 0.0019 0.0002 0.0005 0.0006 0.0002 0.0005 0.0001 0.0002
Endosome 0.0164 0.0009 0.0111 0.0065 0.0432 0.0045 0.034 0.0015 0.0061 0.0001 0.0249 0.0008 0.0072 0.0007 0.0039 0.0029 0 0.0005
Golgi 0.0042 0.0001 0.0069 0.005 0.0105 0.0012 0.0207 0.0001 0.0018 0 0.0328 0.0008 0.002 0 0.0016 0.0031 0 0.0001
Lipid Particles 0.0335 0.0002 0.0049 0.0161 0.0179 0.0098 0.02 0.0002 0.0075 0 0.0362 0.0041 0.0043 0.0004 0.0069 0.0078 0 0.0008
Mitochondria 0.0152 0.0014 0.0029 0.0278 0.0105 0.0045 0.0096 0.0003 0.0015 0.0092 0.0213 0.0004 0.0015 0.0003 0.0003 0.0014 0.0002 0.0001
None 0.0081 0.0029 0.0025 0.0003 0.0007 0.0006 0.0214 0.0069 0.0003 0.0001 0.0002 0.0001 0.0024 0.0139 0.0201 0.0063 0.0001 0.0029
Nuclear Periphery 0.0207 0.009 0.0041 0.0051 0.0049 0.0047 0.014 0.0168 0.0045 0.0014 0.0102 0.001 0.003 0.0209 0.0022 0.009 0.0044 0.0154
Nucleolus 0.0272 0.0109 0.0096 0.0042 0.0353 0.0278 0.0048 0.0089 0.0093 0.005 0.0075 0.0147 0.0155 0.0073 0.0053 0.0046 0.0066 0.0122
Nucleus 0.7776 0.9351 0.9052 0.806 0.8124 0.8913 0.7271 0.9392 0.9248 0.9817 0.7469 0.9539 0.8763 0.9395 0.8876 0.8733 0.9849 0.9313
Peroxisomes 0.0151 0.0003 0.0026 0.015 0.011 0.0019 0.0107 0.0001 0.0065 0.0002 0.0246 0.0105 0.0042 0 0.0028 0.0138 0 0
Punctate Nuclear 0.0302 0.0326 0.0269 0.0879 0.0073 0.0472 0.0099 0.0118 0.0006 0.0002 0.0006 0.0016 0.0067 0.0108 0.0065 0.0037 0.0008 0.0139
Vacuole 0.0072 0.0007 0.0048 0.0022 0.011 0.0006 0.0069 0.0021 0.0015 0.0002 0.0342 0.0012 0.0152 0.0004 0.0004 0.0004 0.0001 0.0005
Vacuole Periphery 0.0022 0.0002 0.0018 0.0028 0.0023 0.0006 0.0027 0.0004 0.0004 0.0002 0.0169 0.0001 0.0029 0.0003 0.0001 0.0005 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.7719 7.3316 6.2773 11.109 13.9053 11.98 6.8427 7.5048 9.8439 17.2399
Translational Efficiency 1.0939 1.032 0.9506 0.7121 0.8761 1.6122 1.0349 1.0612 0.9657 0.7765

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2162 1026 918 1063 1140 955 489 846 3302 1981 1407 1909

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 638.53 687.28 928.65 831.91 682.77 740.42 903.20 830.23 653.80 712.90 919.80 831.17
Standard Deviation 345.93 118.54 115.60 114.68 83.24 103.12 101.53 124.50 284.94 114.49 111.58 119.13
Intensity Change Log 2 — 0.106144 0.540381 0.381673 — 0.116944 0.403646 0.282111 — 0.111735 0.471343 0.331084

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000066 0.000331 0.000607 0.000769 0.000128 0.001333 0.000617 0.000501 0.000087 0.000814 0.000610 0.000650
Bud Neck 0.003979 0.003763 0.007097 0.012166 0.002398 0.007326 0.006362 0.009816 0.003433 0.005480 0.006842 0.011124
Bud Site 0.002764 0.001695 0.001942 0.013093 0.001048 0.003648 0.000930 0.008681 0.002171 0.002636 0.001590 0.011138
Cell Periphery 0.000296 0.000193 0.000116 0.000198 0.000092 0.000117 0.000068 0.000135 0.000225 0.000157 0.000100 0.000170
Cytoplasm 0.143150* 0.055397 0.018452 0.063071 0.033185 0.038211 0.010473 0.039323 0.105185* 0.047112 0.015679 0.052547
Cytoplasmic Foci 0.009570 0.008021 0.000656 0.005148 0.003801 0.005658 0.000072 0.001727 0.007578 0.006882 0.000453 0.003632
Eisosomes 0.000020 0.000027 0.000032 0.000028 0.000017 0.000026 0.000025 0.000019 0.000019 0.000026 0.000030 0.000024
Endoplasmic Reticulum 0.001055 0.003899 0.006624 0.006344 0.001668 0.004530 0.006593 0.006505 0.001266 0.004203 0.006614 0.006415
Endosome 0.000514 0.001162 0.000643 0.007522 0.000404 0.002220 0.000270 0.003000 0.000476 0.001672 0.000514 0.005518
Golgi 0.000098 0.001051 0.000061 0.002213 0.000205 0.000689 0.000042 0.001518 0.000135 0.000876 0.000054 0.001905
Lipid Particles 0.000674 0.002488 0.000567 0.000614 0.001429 0.001632 0.000185 0.000293 0.000935 0.002075 0.000434 0.000472
Mitochondria 0.001055 0.002770 0.000964 0.003955 0.001395 0.005218 0.000686 0.001611 0.001173 0.003950 0.000867 0.002916
Mitotic Spindle 0.000337 0.002760 0.001949 0.038631 0.001565 0.010868 0.000475 0.006899 0.000761 0.006669 0.001437 0.024568
None 0.024926 0.023047 0.004067 0.008649 0.012858 0.004722 0.003891 0.006574 0.020760 0.014213 0.004005 0.007729
Nuclear Periphery 0.000935 0.001279 0.000847 0.002180 0.000795 0.002494 0.000643 0.001103 0.000887 0.001865 0.000776 0.001702
Nuclear Periphery Foci 0.000277 0.000582 0.000318 0.001309 0.000487 0.000534 0.000133 0.000521 0.000349 0.000559 0.000254 0.000959
Nucleolus 0.003777 0.008085 0.002097 0.001392 0.005711 0.004987 0.001417 0.001014 0.004445 0.006591 0.001861 0.001225
Nucleus 0.803822* 0.878981* 0.945707* 0.809450* 0.931232* 0.893071* 0.963133* 0.896274* 0.847810* 0.885774* 0.951763* 0.847927*
Peroxisomes 0.000272 0.001268 0.000177 0.000797 0.000461 0.001931 0.000091 0.000131 0.000337 0.001587 0.000147 0.000502
Vacuole 0.002038 0.002912 0.006931 0.019424 0.000945 0.008464 0.003823 0.012000 0.001661 0.005588 0.005851 0.016134
Vacuole Periphery 0.000377 0.000289 0.000145 0.003048 0.000178 0.002321 0.000072 0.002356 0.000308 0.001269 0.000119 0.002742

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.94 -13.78 -12.81 -6.54 -1.18 -1.31 -9.55 -11.24 0.67 1.55 -1.72 -16.77 -16.99 0.04 0.02
Bud Neck -0.39 -3.58 -9.73 -9.48 -4.42 -5.21 -6.68 -7.03 -2.97 -3.28 -4.43 -5.83 -11.51 -7.78 -5.30
Bud Site 1.09 0.29 -4.11 -4.80 -4.23 -3.15 -1.02 -3.96 -1.83 -3.77 -1.31 0.03 -5.46 -4.69 -5.47
Cell Periphery 1.77 5.11 4.19 0.98 -1.75 -1.13 1.10 -1.17 0.16 -1.96 1.97 5.22 3.81 0.88 -2.16
Cytoplasm 14.54 27.27 14.09 -0.52 -9.52 -1.42 6.11 -1.40 0.01 -6.66 13.69 26.54 13.05 -0.59 -11.45
Cytoplasmic Foci 0.42 10.67 6.90 4.07 -3.24 -1.38 5.45 4.03 5.55 -2.83 0.23 11.96 8.41 6.17 -3.91
Eisosomes -4.19 -8.00 -13.16 -6.23 -3.18 -3.87 -5.50 -5.96 -0.41 0.35 -5.66 -9.66 -13.99 -5.01 -2.01
Endoplasmic Reticulum -10.04 -16.98 -21.64 -10.85 -3.23 -8.22 -13.06 -14.40 -5.57 -0.11 -13.14 -21.35 -25.87 -11.63 -2.47
Endosome -2.32 -2.59 -3.62 -2.59 -3.28 -3.44 0.63 -2.68 0.67 -2.78 -4.11 -2.20 -4.40 -1.74 -4.07
Golgi -1.24 1.18 -2.78 0.18 -2.86 -2.13 2.17 -1.37 -0.14 -1.83 -1.80 2.47 -2.95 0.09 -3.29
Lipid Particles -2.03 0.07 -1.25 1.89 -1.27 -0.34 3.09 2.75 4.13 -3.22 -2.25 2.92 2.36 3.17 -1.53
Mitochondria -1.86 -0.07 -2.49 0.14 -2.55 -2.98 1.39 -0.55 2.89 -3.63 -3.60 0.91 -2.58 2.14 -3.38
Mitotic Spindle -2.29 -1.97 -7.53 -6.44 -6.83 -4.05 0.42 -3.88 0.60 -4.17 -4.80 -1.51 -8.53 -4.56 -7.86
None 0.47 10.99 9.24 6.21 -3.14 4.37 5.24 3.80 -0.87 -2.09 3.23 12.15 10.01 5.32 -3.71
Nuclear Periphery -1.62 -0.65 -9.88 -8.67 -12.55 -2.35 -1.98 -8.59 0.70 -7.32 -2.69 -1.16 -11.41 -2.77 -14.33
Nuclear Periphery Foci -3.22 -2.14 -4.43 -1.78 -3.42 -1.07 2.58 0.38 1.48 -2.76 -3.23 -0.16 -3.90 -0.74 -3.89
Nucleolus -4.46 2.89 4.19 7.02 1.36 0.89 7.55 8.31 5.19 0.94 -3.22 6.39 8.26 8.67 1.91
Nucleus -7.58 -19.93 0.51 6.70 15.19 5.23 -3.58 6.00 0.40 9.07 -4.48 -19.07 1.92 5.60 17.15
Peroxisomes -3.50 1.97 -0.79 2.97 -1.50 -2.89 4.41 4.23 3.69 -0.62 -4.42 4.61 1.23 4.67 -1.44
Vacuole -2.86 -6.29 -13.20 -12.36 -10.05 -5.84 -4.52 -9.50 -4.18 -5.11 -6.35 -7.72 -15.90 -12.42 -11.08
Vacuole Periphery 0.68 1.97 -2.19 -2.45 -2.81 -2.31 2.19 -1.85 1.01 -2.04 -2.11 2.39 -2.78 -0.03 -3.39
Standard name
Human Ortholog
Description Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response
Localization
Cell Percentages nucleus (87%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Dot1

Dot1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dot1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available