Standard name
Human Ortholog
Description Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.99 0.98 0.93 0.92 0.88 – 0.76 0.8 0.72 0.74 0.99 0.99 1.0 0.92 0.96 0.99 0.95 0.95 0.97 0.95 0.96 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.18 0 – 0.26 0.14 0.3 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0.12 – 0.11 0.09 0.08 0.06 0 0 0 0.05 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1
Bud 1 0 0 0 0 3 0 – 1 1 1 2 0 0 0 0 0 0 0 2 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 0 2 4 4 2 3 – 1 5 3 7 2 1 4 0 3 3 0 0 0 0 0 0
Cytoplasm 307 527 232 211 309 219 291 – 288 333 148 160 392 376 482 85 93 67 301 497 221 199 362 399
Endoplasmic Reticulum 0 2 2 0 1 0 0 – 0 3 0 2 3 2 2 1 3 2 0 1 0 0 2 2
Endosome 1 0 0 1 0 0 0 – 1 1 0 1 0 0 0 0 0 0 5 1 1 0 2 1
Golgi 0 0 1 1 0 0 0 – 0 0 0 0 0 0 0 0 1 0 2 1 0 0 1 1
Mitochondria 2 3 0 2 3 43 13 – 98 59 62 47 0 0 0 4 1 1 0 2 0 2 1 0
Nucleus 3 2 0 0 1 8 7 – 8 16 6 10 1 4 0 0 2 0 1 5 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 1 1 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 1 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 – 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 5 3 3 6 23 2 38 – 40 38 17 12 0 0 2 5 9 0 0 2 0 1 0 5
Unique Cell Count 315 534 234 216 331 239 330 379 418 205 216 394 380 484 92 97 68 317 522 229 210 379 416
Labelled Cell Count 323 537 240 225 342 277 353 439 457 238 245 398 383 490 95 112 73 317 522 229 210 379 416


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 8.2 8.2 8.0 7.9 5.1 6.5 – 5.6 6.0 5.6 6.1 9.1 7.9 8.9 8.8 9.2 9.0 8.1 8.6 8.6
Std Deviation (1e-4) 1.6 1.4 1.2 1.5 1.5 1.5 1.5 – 1.1 1.5 1.3 2.1 1.6 1.5 1.4 1.6 1.7 1.6 1.5 1.7 1.7
Intensity Change (Log2) – – – -0.02 -0.04 -0.67 -0.33 – -0.55 -0.44 -0.54 -0.43 0.16 -0.05 0.12 0.11 0.18 0.14 -0.02 0.08 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -3.4 -3.9 -4.9 – -7.8 -7.0 -8.3 -7.9 0.5 -0.2 0.7 -3.3 -2.0 -0.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.8 3.1 – 8.5 6.0 9.1 7.5 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 3.0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.2 0 4.6 – 4.4 3.9 3.5 2.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4287 2.5853 2.2664 1.7521 1.7144 2.1081 2.5809 3.9911 3.6498 2.8728 3.3113 3.752 5.8387 6.9733 6.5606 6.2287 6.1878 6.6161
Actin 0.0091 0.0004 0.0002 0.0147 0.0001 0.0005 0.0109 0.0031 0.0062 0.0008 0.0002 0.0033 0.0264 0.0044 0.0085 0.0081 0.0007 0.0037
Bud 0.0038 0.0003 0.0006 0.0002 0.0001 0.0002 0.0002 0.0003 0.0016 0.0002 0 0.0003 0.0011 0.0012 0.0009 0.0002 0.0003 0.0006
Bud Neck 0.001 0.0003 0.0003 0.0222 0.0003 0.0007 0.001 0.0002 0.0005 0.0005 0.0002 0.0047 0.009 0.0002 0.0012 0.0004 0.0028 0.0009
Bud Periphery 0.0008 0.0001 0.0001 0.0001 0.0001 0.0003 0.0003 0.0002 0.0009 0.0001 0 0.0004 0.0026 0.0005 0.0011 0.0001 0.0002 0.0002
Bud Site 0.0047 0.0028 0.0003 0.0019 0 0.0001 0.0005 0.0018 0.0012 0.0001 0 0.0002 0.006 0.0013 0.0057 0.0006 0.0091 0.0001
Cell Periphery 0.0002 0.0002 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0 0 0 0.0003 0.0001 0.0002 0.0001 0.0001 0
Cytoplasm 0.6813 0.8141 0.8478 0.7436 0.7843 0.8479 0.8225 0.9111 0.8723 0.9043 0.9232 0.928 0.7413 0.9242 0.8876 0.8654 0.8679 0.9273
Cytoplasmic Foci 0.0109 0.0079 0.0075 0.0222 0.0061 0.0124 0.0099 0.0068 0.0103 0.0073 0.0037 0.0073 0.0266 0.0089 0.0191 0.0224 0.0259 0.0082
Eisosomes 0.0002 0.0001 0 0.0001 0 0 0.0002 0 0 0 0 0 0.0002 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0031 0.0034 0.0023 0.0034 0.0006 0.0025 0.0045 0.0016 0.0026 0.0055 0.0016 0.0019 0.0024 0.0022 0.0013 0.0013 0.0028 0.0017
Endosome 0.0056 0.0041 0.0032 0.0055 0.0012 0.0039 0.0074 0.0024 0.0033 0.0046 0.0019 0.0045 0.0162 0.0025 0.0095 0.0095 0.0091 0.0022
Golgi 0.0011 0.0003 0.0001 0.0032 0.0001 0.0003 0.0014 0.0002 0.0004 0.0003 0.0001 0.0002 0.0098 0.0012 0.0061 0.0114 0.0018 0.0003
Lipid Particles 0.0014 0.0002 0.0003 0.0098 0 0 0.0022 0.0001 0.0002 0.0001 0 0 0.009 0.0005 0.0029 0.0241 0.0037 0
Mitochondria 0.0008 0.0002 0.0002 0.0005 0.0002 0.0004 0.0007 0.0002 0.0003 0.0006 0.0001 0.0003 0.0137 0.0003 0.0068 0.0126 0.0003 0.0003
None 0.2625 0.155 0.1256 0.1385 0.2034 0.1226 0.1217 0.0626 0.0849 0.0695 0.0657 0.0415 0.1153 0.0484 0.0389 0.0173 0.0474 0.0481
Nuclear Periphery 0.0017 0.0013 0.0012 0.0021 0.0004 0.0007 0.0046 0.0009 0.0016 0.0014 0.0005 0.0007 0.0016 0.0005 0.0004 0.0005 0.001 0.0006
Nucleolus 0.001 0.0002 0.0002 0.0003 0 0 0.0001 0.0001 0.0001 0 0 0 0.0003 0 0.0001 0.0001 0.0017 0
Nucleus 0.0043 0.0058 0.0054 0.0036 0.0021 0.0054 0.0044 0.0058 0.0062 0.0026 0.0018 0.0045 0.0024 0.0018 0.0019 0.0018 0.0028 0.004
Peroxisomes 0.0018 0.0001 0.0001 0.0175 0.0002 0.0004 0.0036 0.0005 0.0013 0.0001 0 0.0003 0.0122 0.0009 0.006 0.0232 0.0014 0.0009
Punctate Nuclear 0.0028 0.0016 0.0032 0.0096 0.0005 0.0008 0.0022 0.0013 0.0048 0.0013 0.0003 0.0009 0.002 0.0004 0.001 0.0005 0.0195 0.0005
Vacuole 0.0016 0.0016 0.001 0.0007 0.0003 0.0006 0.0011 0.0007 0.0009 0.0005 0.0004 0.0007 0.0014 0.0005 0.0006 0.0003 0.0014 0.0003
Vacuole Periphery 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0.0002 0.0004 0.0001 0.0001 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.4073 28.9048 24.1297 36.1302 49.7832 52.514 31.5089 44.382 53.4129 56.836
Translational Efficiency 1.0773 1.0973 1.0501 0.848 0.8248 0.8782 1.0167 0.65 0.6218 0.6042

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘