Standard name
Human Ortholog
Description Sulfite reductase beta subunit; involved in amino acid biosynthesis, transcription repressed by methionine

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.99 0.96 0.95 0.92 0.89 0.82 0.72 0.75 0.98 0.99 0.99 0.96 0.95 0.97 0.96 0.95 0.9 0.91 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.05 0.16 0.22 0.21 0.44 0.49 0.5 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 2
Bud 0 0 6 1 7 2 5 14 10 10 9 2 5 14 1 0 0 0 1 0 4 9 9
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 2 1 3
Bud Site 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0
Cell Periphery 5 3 4 5 13 6 3 9 7 4 6 6 4 9 12 10 14 0 0 0 0 0 0
Cytoplasm 190 269 231 258 543 327 654 782 371 326 431 327 476 585 437 270 311 189 255 216 249 432 516
Endoplasmic Reticulum 3 1 1 0 3 2 2 1 2 0 0 0 0 1 14 6 11 1 2 3 4 4 9
Endosome 0 0 0 0 3 0 0 9 0 3 0 0 0 0 1 5 2 0 1 0 0 4 5
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 0 0 0 1
Mitochondria 1 3 7 19 29 55 158 185 198 221 284 1 5 20 16 2 4 1 0 8 4 4 7
Nucleus 1 0 0 1 1 1 1 3 3 2 0 1 1 0 6 8 6 0 0 0 0 2 0
Nuclear Periphery 0 1 0 1 0 0 1 1 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 2 3 2 4 0 0 0 1 0 0 0 0 0 0 1 3
Vac/Vac Membrane 0 2 0 0 7 0 5 9 1 6 7 3 1 0 11 7 8 0 2 5 5 12 4
Unique Cell Count 194 273 236 261 566 343 714 878 454 454 571 333 481 593 456 285 321 197 269 240 274 480 570
Labelled Cell Count 201 280 249 285 606 394 832 1018 597 576 742 341 492 631 501 309 357 197 269 240 274 480 570


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 7.1 5.4 5.2 5.4 4.8 4.3 4.4 3.6 3.5 3.1 6.8 6.6 5.7 7.0 7.6 8.1 6.5 5.9 6.6
Std Deviation (1e-4) 2.2 2.1 1.3 1.4 1.3 1.3 1.4 1.3 1.2 1.1 0.8 1.9 1.8 1.5 1.9 1.9 1.9 1.7 1.5 1.6
Intensity Change (Log2) -0.03 0.02 -0.16 -0.33 -0.3 -0.59 -0.62 -0.77 0.34 0.31 0.08 0.38 0.5 0.6 0.28 0.14 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 -0.2 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.5 0 0 0 0 0 0 0 0 0 0.8 1.3 1.8
Cytoplasm 0.9 -1.4 -1.6 -3.3 -4.2 -6.0 -8.2 -7.5 0.3 1.2 0.8 -1.4 -1.9 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.3 0 2.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.2 1.3 0 0 0 0 0 0 0 0 0.3 0.4 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.2154 29.752 21.2456 20.966 23.0265 26.853 24.083 36.8279 31.6705 34.133 32.794 35.201 10.7066 14.888 15.1093 15.0325 17.3891 15.5383
Actin 0.0413 0.0009 0.0218 0.0056 0.0003 0.0019 0.0079 0.0002 0.0013 0.0024 0.0059 0.0035 0.0364 0.0097 0.0037 0.0043 0.0798 0.008
Bud 0.0012 0.0012 0.0028 0.0059 0.0009 0.0022 0.0011 0.0004 0.0007 0.0036 0.001 0.0007 0.0019 0.0021 0.0038 0.0041 0.0051 0.0007
Bud Neck 0.0023 0.0008 0.0011 0.0035 0.0011 0.005 0.0003 0.0002 0.0003 0.0004 0.0007 0.0007 0.0007 0.0003 0.0004 0.0005 0.0006 0.0009
Bud Periphery 0.0012 0.0004 0.0033 0.0036 0.0003 0.0052 0.0007 0.0001 0.0002 0.0013 0.0017 0.0004 0.0015 0.0005 0.0013 0.0025 0.0034 0.0006
Bud Site 0.0078 0.005 0.0068 0.0581 0.0001 0.0004 0.0015 0.0004 0.0004 0.0003 0.0006 0.0001 0.0037 0.0052 0.0044 0.0023 0.0024 0.0003
Cell Periphery 0.0025 0.0014 0.0033 0.0017 0.0002 0.0015 0.0007 0.0021 0.0002 0.0001 0.0004 0.0001 0.0006 0.0003 0.0004 0.0005 0.0003 0.0002
Cytoplasm 0.6536 0.9623 0.7989 0.8044 0.83 0.8482 0.8217 0.9836 0.9342 0.9603 0.8824 0.9291 0.7271 0.9465 0.9095 0.8694 0.812 0.9066
Cytoplasmic Foci 0.019 0.0022 0.0105 0.0245 0.0062 0.005 0.0085 0.002 0.0067 0.0041 0.0032 0.0059 0.0333 0.0078 0.0107 0.0221 0.0129 0.012
Eisosomes 0.0003 0 0.0003 0 0 0 0.0002 0 0 0 0.0003 0 0.0003 0 0 0 0.0002 0
Endoplasmic Reticulum 0.1139 0.0042 0.027 0.0057 0.0662 0.039 0.0264 0.0014 0.004 0.0015 0.0109 0.0138 0.0748 0.0097 0.0137 0.007 0.0377 0.0248
Endosome 0.0412 0.0023 0.017 0.0153 0.0306 0.0134 0.0135 0.0015 0.0158 0.0097 0.0134 0.0153 0.0376 0.0075 0.0218 0.0379 0.0214 0.0226
Golgi 0.0104 0.0004 0.0046 0.0017 0.0018 0.0018 0.003 0.0001 0.0064 0.0003 0.0022 0.0042 0.0107 0.0014 0.002 0.0065 0.0015 0.0079
Lipid Particles 0.0039 0.0001 0.0027 0.0012 0.0001 0.0002 0.0025 0.0006 0.0028 0 0.0002 0.0002 0.0098 0.0004 0.0012 0.0029 0.0001 0.0005
Mitochondria 0.0093 0.0002 0.0212 0.0021 0.0047 0.0025 0.0366 0.0001 0.0084 0.0002 0.0519 0.0103 0.0048 0.0003 0.0042 0.0012 0.0065 0.0043
None 0.0091 0.0008 0.019 0.0028 0.0005 0.0004 0.0062 0.0006 0.0007 0.0007 0.0005 0.0005 0.0146 0.001 0.0041 0.0014 0.0019 0.0017
Nuclear Periphery 0.0155 0.0011 0.0173 0.0049 0.0092 0.0362 0.04 0.0005 0.002 0.0016 0.0054 0.0036 0.0113 0.001 0.0027 0.003 0.0024 0.0013
Nucleolus 0.001 0.0002 0.0003 0.0011 0.0001 0.0001 0.0006 0 0.0002 0 0 0 0.0005 0 0.0001 0.0001 0 0
Nucleus 0.0091 0.0017 0.0031 0.016 0.0038 0.0037 0.0078 0.0008 0.0012 0.0013 0.0019 0.0017 0.003 0.001 0.0016 0.0021 0.0014 0.0009
Peroxisomes 0.0042 0 0.0007 0.0006 0 0.0001 0.0008 0 0.0004 0 0.0001 0.0001 0.008 0.0002 0.001 0.0034 0.0005 0.0005
Punctate Nuclear 0.0019 0.0001 0.001 0.0017 0.0002 0.0001 0.0015 0 0.0001 0.0001 0.0001 0.0001 0.0051 0.0002 0.0006 0.0005 0.0002 0.0002
Vacuole 0.0443 0.013 0.0273 0.0334 0.0342 0.022 0.0134 0.005 0.0112 0.009 0.0133 0.0071 0.0119 0.0042 0.0108 0.0208 0.0064 0.0046
Vacuole Periphery 0.0069 0.0018 0.0098 0.006 0.0095 0.0111 0.0051 0.0004 0.003 0.0031 0.0039 0.0026 0.0025 0.0005 0.0019 0.0076 0.003 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.1048 30.7602 34.6608 46.6459 31.7253 30.06 26.4363 27.1334 34.2114 34.2841
Translational Efficiency 1.1127 1.1419 0.827 0.7992 0.9734 1.2475 0.7035 0.4923 0.5942 0.7034

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1641 1161 194 995 1330 1822 184 72 2971 2983 378 1067

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 612.05 807.05 1187.67 902.71 773.57 819.94 989.82 954.92 684.36 814.92 1091.36 906.23
Standard Deviation 82.10 166.61 145.46 120.39 132.20 129.03 101.70 130.76 134.16 144.96 160.23 121.82
Intensity Change Log 2 0.399009 0.956413 0.560613 0.083986 0.355634 0.303848 0.231674 0.652134 0.422932

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000165 0.000189 0.000978 0.002190 0.000255 0.000473 0.000139 0.000401 0.000205 0.000363 0.000569 0.002070
Bud Neck 0.023040 0.008416 0.003178 0.005245 0.005474 0.012764 0.001485 0.001916 0.015176 0.011072 0.002354 0.005020
Bud Site 0.022294 0.022851 0.032823 0.037435 0.016832 0.026985 0.003998 0.046978 0.019849 0.025376 0.018792 0.038079
Cell Periphery 0.000379 0.000105 0.000274 0.000116 0.000130 0.000101 0.000124 0.000162 0.000268 0.000103 0.000201 0.000119
Cytoplasm 0.582342* 0.644758* 0.413865* 0.524185* 0.700044* 0.568426* 0.601036* 0.533246* 0.635033* 0.598135* 0.504975* 0.524796*
Cytoplasmic Foci 0.218570* 0.164412 0.028656 0.039674 0.158023 0.184821* 0.038321 0.037574 0.191465* 0.176878 0.033361 0.039532
Eisosomes 0.000279 0.000037 0.000073 0.000036 0.000078 0.000058 0.000058 0.000047 0.000189 0.000050 0.000066 0.000037
Endoplasmic Reticulum 0.001909 0.000775 0.005989 0.001137 0.002545 0.000733 0.001405 0.001425 0.002194 0.000749 0.003758 0.001157
Endosome 0.010849 0.027242 0.006886 0.010840 0.016415 0.033433 0.006391 0.004944 0.013341 0.031024 0.006645 0.010442
Golgi 0.008210 0.004756 0.000279 0.004921 0.004385 0.007657 0.000089 0.000404 0.006498 0.006528 0.000186 0.004616
Lipid Particles 0.004768 0.002057 0.002963 0.001866 0.002541 0.002637 0.001697 0.001182 0.003771 0.002411 0.002347 0.001820
Mitochondria 0.011610 0.001187 0.000243 0.005076 0.002106 0.005943 0.000063 0.041114 0.007355 0.004092 0.000156 0.007508
Mitotic Spindle 0.000200 0.003369 0.006526 0.079100 0.001415 0.017938 0.000728 0.028174 0.000744 0.012268 0.003703 0.075663
None 0.005295 0.004709 0.001649 0.002990 0.002798 0.000828 0.003497 0.003515 0.004177 0.002339 0.002549 0.003025
Nuclear Periphery 0.000141 0.000182 0.001410 0.000846 0.000202 0.000114 0.000699 0.000392 0.000168 0.000141 0.001064 0.000815
Nuclear Periphery Foci 0.000366 0.000926 0.010858 0.002382 0.000799 0.000246 0.003865 0.003263 0.000560 0.000511 0.007454 0.002441
Nucleolus 0.001157 0.001058 0.000205 0.000541 0.000901 0.000671 0.000193 0.000150 0.001042 0.000821 0.000199 0.000514
Nucleus 0.055390 0.022989 0.220952* 0.043797 0.021880 0.015693 0.086792 0.065932 0.040389 0.018533 0.155647* 0.045291
Peroxisomes 0.002158 0.003796 0.001780 0.002886 0.001563 0.004136 0.000213 0.000809 0.001892 0.004004 0.001018 0.002746
Vacuole 0.047044 0.082931 0.260163* 0.228840* 0.058791 0.111817 0.249078* 0.227197* 0.052303 0.100575 0.254767* 0.228729*
Vacuole Periphery 0.003834 0.003253 0.000249 0.005897 0.002822 0.004526 0.000130 0.001177 0.003381 0.004031 0.000191 0.005579

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.28 -8.69 -1.68 -1.63 -0.22 -1.36 0.81 -0.15 1.26 -0.95 -1.66 -6.65 -1.61 -1.30 -0.85
Bud Neck 9.44 14.37 14.66 5.95 0.89 -7.36 8.49 6.73 11.36 0.14 4.17 15.84 14.61 11.28 -0.83
Bud Site -0.16 1.27 2.18 2.21 -0.33 -4.69 9.23 -0.44 0.37 -1.45 -3.45 3.33 1.12 3.31 -2.14
Cell Periphery 12.00 4.29 14.95 2.37 10.27 2.58 0.83 0.69 -0.60 0.18 12.37 4.54 14.69 3.03 9.37
Cytoplasm -5.88 13.31 11.73 14.94 -5.84 13.54 10.20 7.66 3.93 0.75 5.30 15.69 17.92 13.79 -3.14
Cytoplasmic Foci 7.97 42.03 41.54 24.71 -2.91 -4.15 22.06 19.14 23.58 0.17 3.20 44.25 46.17 39.93 -0.74
Eisosomes 13.56 11.07 13.61 -0.19 7.83 0.60 0.27 0.93 1.42 3.03 7.66 6.40 8.45 3.01 9.29
Endoplasmic Reticulum 3.88 -7.06 3.28 -0.97 9.22 2.25 0.50 1.70 -1.43 3.07 3.65 -4.24 3.09 -1.33 9.54
Endosome -7.21 5.23 6.64 10.13 2.25 -9.18 8.16 9.25 19.38 1.74 -12.88 9.64 9.83 19.81 1.95
Golgi 5.33 19.05 10.38 4.61 -2.26 -4.85 17.31 16.12 11.87 -1.56 0.03 24.47 9.62 8.11 -2.42
Lipid Particles 7.32 4.91 10.04 2.60 5.31 -0.20 2.13 4.28 4.78 2.70 4.88 5.35 8.92 4.32 4.56
Mitochondria 8.34 9.23 7.26 -0.51 -1.90 -3.46 3.36 -0.86 -0.58 -1.01 3.54 9.76 3.77 1.37 -1.96
Mitotic Spindle -2.80 -2.37 -7.60 -6.59 -6.03 -6.85 0.19 -2.04 -0.91 -2.06 -7.60 -2.19 -7.74 -4.61 -7.02
None 0.45 4.36 3.46 1.70 -2.00 5.70 0.20 0.65 -1.89 0.40 2.69 3.63 3.43 -0.07 -0.57
Nuclear Periphery -1.07 -13.65 -9.88 -9.25 6.22 1.13 -8.43 -3.64 -5.22 3.86 0.67 -15.67 -9.47 -10.41 5.15
Nuclear Periphery Foci -2.79 -11.88 -4.94 -3.02 10.32 4.96 -5.52 -2.27 -2.89 0.81 0.42 -12.35 -5.25 -5.97 9.78
Nucleolus 0.20 2.79 2.38 3.46 -1.03 0.95 2.75 2.97 2.72 1.99 0.96 3.88 3.19 3.05 -1.11
Nucleus 10.50 -10.50 -2.54 -11.82 9.86 3.98 -8.96 -3.77 -4.33 3.65 12.71 -13.17 -7.84 -15.80 10.48
Peroxisomes -2.82 2.67 3.61 4.18 -0.60 -6.45 6.81 4.17 9.50 -1.07 -6.52 5.00 3.01 7.35 -1.57
Vacuole -9.06 -16.25 -33.55 -28.56 -0.64 -12.77 -13.62 -8.92 -7.46 -0.76 -16.58 -21.13 -34.49 -28.80 -1.22
Vacuole Periphery 1.32 12.74 2.29 1.58 -1.58 -2.72 6.69 4.86 7.49 -1.25 -1.54 13.41 2.05 2.67 -1.66