Standard name
Human Ortholog
Description ER-resident protein involved in peroxisomal biogenesis; ER-localized protein that associates with peroxisomes; interacts with Pex30p and reticulons Rtn1p and Yop1p to regulate peroxisome biogenesis from the ER; role in peroxisomal-destined vesicular flow from the ER; regulates peroxisomal size, number and distribution; Pex28p and Pex29p may act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.32 0.2 – 0.19 0.19 0.15 0.13 0.13 0.08 0.1 0.09 0.06 0.13 0.16 0.13 0.46 0.39 0.4 0.23 0.16 0.16 0.12 0.19 0.19
Bud 0.09 0 – 0 0 0 0.05 0.06 0.07 0.09 0.09 0.07 0 0 0 0.07 0.12 0.1 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0.06
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.08 – 0.09 0.15 0.1 0 0 0 0 0 0 0.07 0.1 0.1 0.22 0.29 0.17 0 0.05 0.08 0.05 0.11 0.14
Cytoplasm 0.25 0.31 – 0.62 0.51 0.49 0.39 0.42 0.38 0.35 0.28 0.36 0.73 0.68 0.61 0.21 0.16 0.29 0.05 0.1 0 0.13 0.12 0.11
Endoplasmic Reticulum 0.39 0.45 – 0.18 0.24 0.25 0.28 0.25 0.18 0.16 0.15 0.1 0.17 0.12 0.2 0.05 0.07 0.06 0.32 0.32 0.18 0.21 0.22 0.29
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Mitochondria 0.25 0 – 0 0 0.18 0.25 0.17 0.37 0.39 0.57 0.47 0 0 0 0.1 0.07 0.11 0.15 0.14 0.27 0.2 0.12 0.08
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0.05 0.07 0.05 0.06 0.07 0 0 0 0 0 0 0.08 0.11 0.09 0.11 0.08 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 41 37 – 35 51 52 77 67 24 42 44 37 48 95 109 85 105 130 40 37 9 15 87 120
Bud 12 5 – 7 10 15 32 30 20 36 41 42 10 23 10 12 31 33 3 2 2 5 13 20
Bud Neck 0 1 – 1 7 1 2 2 1 1 1 0 3 10 3 0 12 0 3 9 3 7 34 36
Bud Site 0 0 – 0 0 0 0 0 2 1 0 2 0 0 0 1 0 2 – – – – – –
Cell Periphery 4 14 – 17 40 35 27 19 9 11 11 17 24 63 88 40 78 56 5 11 4 7 51 86
Cytoplasm 32 56 – 114 134 170 239 208 113 141 130 208 267 411 516 39 43 95 9 22 2 16 53 69
Endoplasmic Reticulum 51 82 – 33 64 87 173 125 53 63 69 58 61 75 174 10 19 20 56 74 10 27 100 186
Endosome 4 0 – 3 0 6 9 12 12 15 2 18 1 4 16 3 5 11 7 7 2 2 9 5
Golgi 0 0 – 2 1 0 0 0 0 0 0 0 0 1 1 9 10 5 4 4 0 1 1 1
Mitochondria 33 4 – 5 6 62 153 84 110 160 269 272 0 6 1 18 20 35 26 32 16 26 56 50
Nucleus 0 2 – 0 1 0 8 6 6 3 1 3 1 3 4 3 5 7 0 1 0 1 2 2
Nuclear Periphery 0 0 – 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 1 0 0 2 0 1 0 3 0 1 10 7 2 0 0 0 0 0
SpindlePole 3 1 – 3 4 2 10 12 2 3 2 7 6 10 46 13 16 18 0 0 0 0 2 0
Vac/Vac Membrane 1 0 – 0 0 7 18 23 20 20 26 42 2 4 16 7 11 11 14 25 5 14 34 43
Unique Cell Count 130 181 185 263 349 612 501 301 406 469 580 368 601 849 183 267 325 176 234 62 132 456 633
Labelled Cell Count 181 202 220 318 438 749 588 372 499 596 708 423 708 984 241 365 430 176 234 62 132 456 633


Ambiguous, Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.3 – 6.1 6.3 5.7 5.7 6.0 5.3 5.4 5.0 5.2 7.0 6.7 7.5 8.7 8.6 7.5 6.4 6.9 7.3
Std Deviation (1e-4) 0.6 0.9 – 1.2 0.9 1.0 1.0 0.9 0.9 0.8 0.9 0.8 1.2 1.3 1.2 1.5 1.5 1.5 1.5 1.6 1.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7475 2.3822 2.663 2.7865 2.5666 2.4159 2.575 2.3274 2.5798 2.5075 2.1892 2.1924 0.4864 0.7499 0.83 0.6962 0.2265 0.9039
Actin 0.0594 0.0392 0.0608 0.0499 0.0439 0.031 0.1323 0.0626 0.171 0.0735 0.0396 0.1013 0.1304 0.0397 0.1444 0.1777 0.0442 0.0554
Bud 0.0059 0.0039 0.0245 0.0052 0.0028 0.0051 0.001 0.0016 0.04 0.0049 0.0007 0.0013 0.005 0.0079 0.0427 0.0085 0.0077 0.001
Bud Neck 0.0268 0.0047 0.0131 0.0302 0.051 0.0825 0.0089 0.0052 0.0073 0.0051 0.0023 0.1374 0.0163 0.0051 0.0104 0.0181 0.0012 0.1246
Bud Periphery 0.0165 0.0084 0.1014 0.0302 0.0094 0.0149 0.0017 0.0045 0.1385 0.0276 0.0022 0.0047 0.0094 0.005 0.1306 0.0537 0.0965 0.0036
Bud Site 0.0361 0.0822 0.0838 0.0044 0.0219 0.0071 0.0269 0.0974 0.0528 0.01 0.0012 0.0035 0.0375 0.1306 0.0658 0.0037 0.0009 0.0021
Cell Periphery 0.0225 0.0227 0.0209 0.0096 0.0125 0.0118 0.0222 0.0378 0.0335 0.0434 0.0101 0.0187 0.0211 0.0146 0.0153 0.0135 0.0051 0.012
Cytoplasm 0.3181 0.4394 0.3286 0.4728 0.4899 0.4626 0.419 0.4293 0.3342 0.4671 0.6403 0.5019 0.3835 0.4675 0.3143 0.3988 0.3517 0.5305
Cytoplasmic Foci 0.0895 0.0417 0.0396 0.026 0.0299 0.0653 0.0497 0.0567 0.0214 0.049 0.029 0.0309 0.0631 0.0437 0.0299 0.0509 0.0291 0.0366
Eisosomes 0.003 0.0025 0.0021 0.0008 0.0014 0.0008 0.002 0.0018 0.001 0.0012 0.0005 0.0012 0.002 0.0009 0.0016 0.0011 0.0007 0.001
Endoplasmic Reticulum 0.0549 0.069 0.058 0.0819 0.0821 0.0573 0.1027 0.0794 0.0398 0.1468 0.1159 0.0681 0.0767 0.0994 0.0657 0.0644 0.0762 0.1031
Endosome 0.0664 0.0432 0.0611 0.0651 0.035 0.0618 0.0798 0.0173 0.0241 0.032 0.0458 0.0279 0.0677 0.0241 0.033 0.0266 0.0598 0.0258
Golgi 0.0318 0.0224 0.0333 0.0133 0.0273 0.0328 0.0305 0.0167 0.0342 0.0316 0.0409 0.0185 0.0345 0.0166 0.0213 0.0421 0.0255 0.0254
Lipid Particles 0.0397 0.024 0.0127 0.0097 0.0055 0.0198 0.0297 0.0274 0.0071 0.0524 0.027 0.0108 0.0511 0.0319 0.0098 0.0128 0.0046 0.0087
Mitochondria 0.0181 0.0097 0.0337 0.1333 0.0279 0.0157 0.0053 0.0016 0.0061 0.0188 0.0044 0.0022 0.0066 0.0014 0.0052 0.0027 0.0018 0.0041
None 0.1384 0.1631 0.0814 0.028 0.1222 0.0942 0.0257 0.146 0.0732 0.0097 0.0189 0.0615 0.0453 0.096 0.0841 0.1164 0.2843 0.0582
Nuclear Periphery 0.0105 0.004 0.0038 0.0042 0.0084 0.0031 0.0103 0.0021 0.0025 0.0023 0.003 0.0012 0.0039 0.0028 0.0064 0.0016 0.0022 0.0013
Nucleolus 0.0053 0.0008 0.0013 0.0006 0.0021 0.0011 0.0029 0.0005 0.0003 0.0003 0.0002 0.0002 0.0117 0.0005 0.0011 0.0001 0.0003 0.0001
Nucleus 0.0084 0.0034 0.0043 0.0028 0.0072 0.003 0.014 0.0013 0.0013 0.0009 0.0008 0.0009 0.006 0.0016 0.0028 0.0007 0.0012 0.0009
Peroxisomes 0.0209 0.0027 0.0061 0.0065 0.0014 0.011 0.0158 0.0019 0.0017 0.0146 0.0092 0.0007 0.0123 0.0016 0.0027 0.0012 0.0003 0.0008
Punctate Nuclear 0.0066 0.002 0.0017 0.0007 0.0014 0.0065 0.0057 0.003 0.0008 0.0009 0.002 0.0006 0.0031 0.0031 0.0033 0.0009 0.0017 0.0008
Vacuole 0.0185 0.0099 0.0245 0.0166 0.0139 0.0106 0.0113 0.0058 0.0081 0.0069 0.005 0.006 0.0105 0.0055 0.0085 0.0037 0.0041 0.0035
Vacuole Periphery 0.0027 0.0011 0.0033 0.0081 0.003 0.0021 0.0027 0.0003 0.001 0.0009 0.0007 0.0007 0.002 0.0004 0.0013 0.0006 0.0009 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6152 6.5166 5.2752 12.116 9.4515 13.9091 14.3758 16.3733 18.0431 14.4102
Translational Efficiency 0.8374 0.9216 1.3232 0.5449 0.6578 0.7403 0.5882 0.5561 0.583 0.6348

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ER-resident protein involved in peroxisomal biogenesis; ER-localized protein that associates with peroxisomes; interacts with Pex30p and reticulons Rtn1p and Yop1p to regulate peroxisome biogenesis from the ER; role in peroxisomal-destined vesicular flow from the ER; regulates peroxisomal size, number and distribution; Pex28p and Pex29p may act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
Localization
Cell Percentages ER (53%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Pex29

Pex29


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex29-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available