Standard name
Description ER-resident protein involved in peroxisomal biogenesis; ER-localized protein that associates with peroxisomes; interacts with Pex30p and reticulons Rtn1p and Yop1p to regulate peroxisome biogenesis from the ER; role in peroxisomal-destined vesicular flow from the ER; regulates peroxisomal size, number and distribution; Pex28p and Pex29p may act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.32 0.2 0.19 0.19 0.15 0.13 0.13 0.08 0.1 0.09 0.06 0.13 0.16 0.13 0.46 0.39 0.4 0.23 0.16 0.16 0.12 0.19 0.19
Bud 0.09 0 0 0 0 0.05 0.06 0.07 0.09 0.09 0.07 0 0 0 0.07 0.12 0.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.08 0.09 0.15 0.1 0 0 0 0 0 0 0.07 0.1 0.1 0.22 0.29 0.17 0 0.05 0.08 0.05 0.11 0.14
Cytoplasm 0.25 0.31 0.62 0.51 0.49 0.39 0.42 0.38 0.35 0.28 0.36 0.73 0.68 0.61 0.21 0.16 0.29 0.05 0.1 0 0.13 0.12 0.11
Endoplasmic Reticulum 0.39 0.45 0.18 0.24 0.25 0.28 0.25 0.18 0.16 0.15 0.1 0.17 0.12 0.2 0.05 0.07 0.06 0.32 0.32 0.18 0.21 0.22 0.29
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Mitochondria 0.25 0 0 0 0.18 0.25 0.17 0.37 0.39 0.57 0.47 0 0 0 0.1 0.07 0.11 0.15 0.14 0.27 0.2 0.12 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.07 0.05 0.06 0.07 0 0 0 0 0 0 0.08 0.11 0.09 0.11 0.08 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 41 37 35 51 52 77 67 24 42 44 37 48 95 109 85 105 130 40 37 9 15 87 120
Bud 12 5 7 10 15 32 30 20 36 41 42 10 23 10 12 31 33 3 2 2 5 13 20
Bud Neck 0 1 1 7 1 2 2 1 1 1 0 3 10 3 0 12 0 3 9 3 7 34 36
Bud Site 0 0 0 0 0 0 0 2 1 0 2 0 0 0 1 0 2
Cell Periphery 4 14 17 40 35 27 19 9 11 11 17 24 63 88 40 78 56 5 11 4 7 51 86
Cytoplasm 32 56 114 134 170 239 208 113 141 130 208 267 411 516 39 43 95 9 22 2 16 53 69
Endoplasmic Reticulum 51 82 33 64 87 173 125 53 63 69 58 61 75 174 10 19 20 56 74 10 27 100 186
Endosome 4 0 3 0 6 9 12 12 15 2 18 1 4 16 3 5 11 7 7 2 2 9 5
Golgi 0 0 2 1 0 0 0 0 0 0 0 0 1 1 9 10 5 4 4 0 1 1 1
Mitochondria 33 4 5 6 62 153 84 110 160 269 272 0 6 1 18 20 35 26 32 16 26 56 50
Nucleus 0 2 0 1 0 8 6 6 3 1 3 1 3 4 3 5 7 0 1 0 1 2 2
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 2 0 1 0 3 0 1 10 7 2 0 0 0 0 0
SpindlePole 3 1 3 4 2 10 12 2 3 2 7 6 10 46 13 16 18 0 0 0 0 2 0
Vac/Vac Membrane 1 0 0 0 7 18 23 20 20 26 42 2 4 16 7 11 11 14 25 5 14 34 43
Unique Cell Count 130 181 185 263 349 612 501 301 406 469 580 368 601 849 183 267 325 176 234 62 132 456 633
Labelled Cell Count 181 202 220 318 438 749 588 372 499 596 708 423 708 984 241 365 430 176 234 62 132 456 633


Ambiguous, Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.3 6.1 6.3 5.7 5.7 6.0 5.3 5.4 5.0 5.2 7.0 6.7 7.5 8.7 8.6 7.5 6.4 6.9 7.3
Std Deviation (1e-4) 0.6 0.9 1.2 0.9 1.0 1.0 0.9 0.9 0.8 0.9 0.8 1.2 1.3 1.2 1.5 1.5 1.5 1.5 1.6 1.4
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole