Standard name
Human Ortholog
Description Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.12 0.09 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.07 0.21 0.06 0.07 0.13 0.18 0.19 0.17 0.22 0.16 0.16 0.16 0.05 0.23 0.28 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0.2 0.15 0.05 – – – 0 0 0 0 0 0
Endosome 0 0 0.2 0.37 0.23 0.18 0.14 0.2 0.19 0.13 0.14 0.24 0.25 0.22 0.28 – – – 0.12 0.15 0.3 0.28 0.19 0.14
Golgi 0 0 0.08 0.2 0.11 0.09 0.07 0 0 0 0 0.07 0.14 0.07 0 – – – 0.07 0 0.29 0.33 0.21 0.16
Mitochondria 0.14 0.38 0.28 0.28 0.13 0.26 0.24 0.18 0.36 0.41 0.42 0.35 0.15 0 0 – – – 0.05 0 0 0.08 0.08 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.06 0.06 0.05 0.07 0.05 0.05 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.07 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.84 0.61 0.63 0.35 0.61 0.46 0.51 0.47 0.32 0.37 0.26 0.23 0.32 0.34 0.27 – – – 0.73 0.73 0.34 0.27 0.48 0.61
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 4 2 3 2 4 9 2 15 13 25 27 20 – – – 0 0 3 1 2 1
Bud 0 1 3 6 2 6 5 6 15 11 25 27 3 7 5 – – – 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 2 2 0 0 0 – – – – – – – – –
Cell Periphery 0 0 2 0 1 0 0 3 3 1 5 4 2 2 1 – – – 0 0 0 0 0 0
Cytoplasm 4 21 28 21 53 73 101 112 106 69 70 79 12 53 63 – – – 0 0 0 0 1 5
Endoplasmic Reticulum 3 3 8 2 5 9 12 21 5 11 11 11 46 35 12 – – – 0 0 0 1 5 7
Endosome 1 0 92 113 96 72 72 127 90 55 61 121 59 50 63 – – – 11 17 146 81 108 78
Golgi 0 0 35 61 47 37 38 26 20 8 13 36 32 16 10 – – – 6 5 142 95 123 89
Mitochondria 8 38 124 87 53 107 127 114 172 178 185 176 34 8 10 – – – 4 5 17 23 43 25
Nucleus 0 1 1 0 0 1 1 5 4 2 1 1 0 2 2 – – – 0 0 0 0 1 1
Nuclear Periphery 1 0 3 0 2 1 5 6 1 2 2 4 3 1 0 – – – 1 0 3 1 3 1
Nucleolus 0 0 4 5 13 26 32 39 26 30 21 25 0 0 1 – – – 0 0 0 0 0 0
Peroxisomes 0 0 5 1 3 7 4 11 5 3 8 6 0 0 2 – – – 0 0 2 5 2 0
SpindlePole 0 0 6 11 4 5 12 11 13 7 10 23 12 7 16 – – – 0 0 1 0 0 0
Vac/Vac Membrane 49 62 283 108 251 186 270 300 154 160 115 119 75 77 61 – – – 69 88 167 79 275 343
Unique Cell Count 58 101 450 308 413 408 530 643 480 438 440 509 234 226 225 96 121 487 291 575 561
Labelled Cell Count 66 126 595 419 532 533 681 785 623 540 544 647 303 285 266 96 121 487 291 575 561


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.4 7.7 9.7 10.7 9.9 9.4 9.4 9.1 8.3 8.8 8.8 7.8 7.3 6.9 – – – 9.7 9.0 9.6
Std Deviation (1e-4) 1.0 1.3 1.4 1.7 2.2 2.1 2.1 2.2 2.2 2.0 1.9 1.7 0.9 0.8 0.9 – – – 1.8 1.7 2.1
Intensity Change (Log2) – – – 0.34 0.48 0.36 0.3 0.3 0.25 0.11 0.2 0.2 0.03 -0.07 -0.15 – – – 0.34 0.24 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 3.6 3.0 6.8 7.2 6.1 – – –
Bud 0 0 0 0 0 2.7 2.2 4.3 4.1 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0.3 3.3 5.3 5.9 5.5 6.9 4.6 4.6 4.6 -0.6 6.5 7.8 – – –
Endoplasmic Reticulum 0 -0.7 0.4 0.5 1.5 -1.0 0.8 0.7 0.4 8.2 6.9 2.6 – – –
Endosome 4.9 1.0 -1.0 -2.9 -0.3 -0.7 -3.2 -2.6 1.2 1.4 0.5 2.2 – – –
Golgi 4.9 1.8 0.7 -0.4 -2.6 -2.3 -4.1 -3.2 -0.4 2.5 -0.3 -1.6 – – –
Mitochondria 0.2 -5.4 -0.4 -1.3 -3.9 2.7 4.1 4.5 2.3 -3.8 -7.4 -7.1 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 2.4 4.4 4.3 4.3 3.9 4.6 3.5 3.6 0 0 0 – – –
Peroxisomes 0 0 0.8 0 0.8 0 0 0.9 0.1 0 0 0 – – –
SpindlePole 2.0 0 -0.1 1.1 0.5 1.5 0.3 1.1 2.9 2.9 0 4.0 – – –
Vacuole -7.5 -0.6 -5.1 -3.8 -5.3 -9.4 -7.9 -11.0 -12.4 -7.7 -7.1 -8.8 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.3461 9.2326 8.7059 6.9498 7.3772 8.4984 8.2038 10.4318 9.8245 8.6096 8.4531 9.969 9.4995 11.6953 10.16 9.4912 9.5081 10.46
Actin 0.0122 0.0013 0.0011 0.009 0.0003 0.0007 0.0206 0.0007 0.0076 0.0004 0.0122 0.0039 0.0388 0.0003 0.0078 0.0608 0.0004 0.0026
Bud 0.0001 0.0001 0.0001 0.0006 0 0.0002 0.0004 0.0001 0.0002 0.0001 0.0002 0.0007 0.0004 0.0002 0.0003 0.0002 0.0006 0.0002
Bud Neck 0.0003 0.0003 0.0002 0.0108 0.0001 0.0003 0.001 0.0002 0.0004 0.0001 0.0003 0.0004 0.0007 0.0002 0.0005 0.0003 0.0005 0.0005
Bud Periphery 0.0002 0.0002 0.0001 0.0007 0 0.0003 0.0005 0.0001 0.0003 0.0001 0.0002 0.0015 0.0005 0.0004 0.0004 0.0003 0.0017 0.0004
Bud Site 0.0002 0.0002 0.0001 0.0019 0 0.0002 0.0029 0.0001 0.0004 0.0002 0.0003 0.0003 0.0016 0.0002 0.0003 0.0005 0.0017 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0003 0.0004 0.0002 0.0002 0.0002 0.0013 0.0003
Cytoplasm 0.0013 0.0045 0.0021 0.0011 0.0006 0.0011 0.0021 0.002 0.0019 0.0038 0.0013 0.0009 0.0021 0.0012 0.001 0.0014 0.001 0.0008
Cytoplasmic Foci 0.0398 0.033 0.0292 0.0281 0.0306 0.0217 0.0453 0.0231 0.0256 0.0204 0.0301 0.0183 0.0229 0.0178 0.0141 0.0207 0.0207 0.0119
Eisosomes 0 0 0 0.0001 0 0 0.0002 0 0.0002 0 0.0001 0 0.0003 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0016 0.0022 0.0107 0.0007 0.0008 0.002 0.004 0.0035 0.0032 0.0031 0.0023 0.0029 0.0041 0.0029 0.0023 0.003 0.0013 0.0027
Endosome 0.3669 0.4576 0.4234 0.4752 0.4044 0.3947 0.3238 0.4305 0.3386 0.461 0.5178 0.334 0.3549 0.3605 0.3574 0.437 0.5384 0.3341
Golgi 0.4583 0.4108 0.4426 0.3165 0.4684 0.3715 0.4501 0.3752 0.4628 0.3077 0.2975 0.4671 0.3676 0.4001 0.4063 0.2346 0.1969 0.4375
Lipid Particles 0.037 0.0046 0.0087 0.0064 0.0052 0.0138 0.027 0.0051 0.0061 0.0052 0.0253 0.0144 0.0256 0.0067 0.0066 0.0129 0.009 0.0249
Mitochondria 0.0266 0.0086 0.0124 0.0347 0.0026 0.0158 0.0572 0.0286 0.0575 0.0355 0.0218 0.0388 0.0965 0.0378 0.0246 0.0166 0.0113 0.0277
None 0.0001 0 0 0.0001 0 0.0001 0.0003 0 0.0001 0.0113 0.0007 0.0001 0.0005 0.0001 0.0001 0.001 0.0002 0.0001
Nuclear Periphery 0.005 0.0012 0.001 0.0011 0.0011 0.0029 0.0019 0.0037 0.0036 0.0023 0.0023 0.0021 0.0014 0.0036 0.0034 0.0019 0.0015 0.002
Nucleolus 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0017 0.0001
Nucleus 0.0002 0.0002 0.0001 0.0002 0.0001 0.0004 0.0003 0.0002 0.0003 0.0003 0.0003 0.0002 0.0003 0.0003 0.0003 0.0002 0.0012 0.0003
Peroxisomes 0.0054 0.0007 0.0005 0.0061 0.0004 0.0006 0.0144 0.0002 0.0016 0.0012 0.0034 0.0021 0.0054 0.0004 0.0009 0.0078 0.0013 0.0004
Punctate Nuclear 0.0003 0.0001 0.0001 0.0007 0 0.0001 0.0006 0 0.0001 0.0002 0.0027 0.0001 0.0002 0 0.0001 0.0007 0.0002 0.0001
Vacuole 0.0212 0.0412 0.0329 0.0509 0.0341 0.0817 0.0192 0.0501 0.0408 0.0484 0.0267 0.0426 0.0295 0.0672 0.0683 0.0692 0.115 0.0578
Vacuole Periphery 0.0232 0.033 0.0346 0.0546 0.0508 0.0917 0.0277 0.0765 0.0486 0.0984 0.0542 0.0693 0.046 0.0996 0.1051 0.1302 0.0939 0.0954

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 249.9057 201.8768 205.9768 204.6771 207.7563 241.4695 214.6235 184.3627 203.4436 211.2997
Translational Efficiency 0.9139 0.8773 0.832 0.8172 0.7739 0.8609 0.7304 0.8863 0.8608 0.7876

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
Localization
Cell Percentages Golgi (21%), vacuole (15%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kre2

Kre2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kre2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available