Standard name
Description Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.12 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.21 0.06 0.07 0.13 0.18 0.19 0.17 0.22 0.16 0.16 0.16 0.05 0.23 0.28 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0.2 0.15 0.05 0 0 0 0 0 0
Endosome 0 0 0.2 0.37 0.23 0.18 0.14 0.2 0.19 0.13 0.14 0.24 0.25 0.22 0.28 0.12 0.15 0.3 0.28 0.19 0.14
Golgi 0 0 0.08 0.2 0.11 0.09 0.07 0 0 0 0 0.07 0.14 0.07 0 0.07 0 0.29 0.33 0.21 0.16
Mitochondria 0.14 0.38 0.28 0.28 0.13 0.26 0.24 0.18 0.36 0.41 0.42 0.35 0.15 0 0 0.05 0 0 0.08 0.08 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.06 0.06 0.05 0.07 0.05 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.84 0.61 0.63 0.35 0.61 0.46 0.51 0.47 0.32 0.37 0.26 0.23 0.32 0.34 0.27 0.73 0.73 0.34 0.27 0.48 0.61
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 4 2 3 2 4 9 2 15 13 25 27 20 0 0 3 1 2 1
Bud 0 1 3 6 2 6 5 6 15 11 25 27 3 7 5 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 2 2 0 0 0
Cell Periphery 0 0 2 0 1 0 0 3 3 1 5 4 2 2 1 0 0 0 0 0 0
Cytoplasm 4 21 28 21 53 73 101 112 106 69 70 79 12 53 63 0 0 0 0 1 5
Endoplasmic Reticulum 3 3 8 2 5 9 12 21 5 11 11 11 46 35 12 0 0 0 1 5 7
Endosome 1 0 92 113 96 72 72 127 90 55 61 121 59 50 63 11 17 146 81 108 78
Golgi 0 0 35 61 47 37 38 26 20 8 13 36 32 16 10 6 5 142 95 123 89
Mitochondria 8 38 124 87 53 107 127 114 172 178 185 176 34 8 10 4 5 17 23 43 25
Nucleus 0 1 1 0 0 1 1 5 4 2 1 1 0 2 2 0 0 0 0 1 1
Nuclear Periphery 1 0 3 0 2 1 5 6 1 2 2 4 3 1 0 1 0 3 1 3 1
Nucleolus 0 0 4 5 13 26 32 39 26 30 21 25 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 5 1 3 7 4 11 5 3 8 6 0 0 2 0 0 2 5 2 0
SpindlePole 0 0 6 11 4 5 12 11 13 7 10 23 12 7 16 0 0 1 0 0 0
Vac/Vac Membrane 49 62 283 108 251 186 270 300 154 160 115 119 75 77 61 69 88 167 79 275 343
Unique Cell Count 58 101 450 308 413 408 530 643 480 438 440 509 234 226 225 96 121 487 291 575 561
Labelled Cell Count 66 126 595 419 532 533 681 785 623 540 544 647 303 285 266 96 121 487 291 575 561


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.4 7.7 9.7 10.7 9.9 9.4 9.4 9.1 8.3 8.8 8.8 7.8 7.3 6.9 9.7 9.0 9.6
Std Deviation (1e-4) 1.0 1.3 1.4 1.7 2.2 2.1 2.1 2.2 2.2 2.0 1.9 1.7 0.9 0.8 0.9 1.8 1.7 2.1
Intensity Change (Log2) 0.34 0.48 0.36 0.3 0.3 0.25 0.11 0.2 0.2 0.03 -0.07 -0.15 0.34 0.24 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 3.6 3.0 6.8 7.2 6.1
Bud 0 0 0 0 0 2.7 2.2 4.3 4.1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 3.3 5.3 5.9 5.5 6.9 4.6 4.6 4.6 -0.6 6.5 7.8
Endoplasmic Reticulum 0 -0.7 0.4 0.5 1.5 -1.0 0.8 0.7 0.4 8.2 6.9 2.6
Endosome 4.9 1.0 -1.0 -2.9 -0.3 -0.7 -3.2 -2.6 1.2 1.4 0.5 2.2
Golgi 4.9 1.8 0.7 -0.4 -2.6 -2.3 -4.1 -3.2 -0.4 2.5 -0.3 -1.6
Mitochondria 0.2 -5.4 -0.4 -1.3 -3.9 2.7 4.1 4.5 2.3 -3.8 -7.4 -7.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.4 4.4 4.3 4.3 3.9 4.6 3.5 3.6 0 0 0
Peroxisomes 0 0 0.8 0 0.8 0 0 0.9 0.1 0 0 0
SpindlePole 2.0 0 -0.1 1.1 0.5 1.5 0.3 1.1 2.9 2.9 0 4.0
Vacuole -7.5 -0.6 -5.1 -3.8 -5.3 -9.4 -7.9 -11.0 -12.4 -7.7 -7.1 -8.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kre2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available