Standard name
Human Ortholog
Description Myo-inositol transporter; member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress; ITR1 has a paralog, ITR2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.07 0.08 0.08 0 0 – 0 0 0 0 0 0.1 0.12 0.13 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0.47 0.34 0.73 0.53 0.26 0.09 – 0 0 0 0 0 0.77 0.77 0.81 0 0.11 – 0.25 0.19 0.64 0.26 0.35 0.41
Cytoplasm 0.19 0.21 0.09 0.2 0.18 0.3 – 0.13 0.27 0.27 0.37 0.35 0.09 0.09 0.07 0.12 0.15 – 0.11 0.13 0 0.12 0 0
Endoplasmic Reticulum 0.43 0.52 0.22 0.36 0.51 0.39 – 0.07 0.09 0.08 0.05 0.08 0.2 0.21 0.1 0.05 0.05 – 0.34 0.38 0.18 0.3 0.34 0.32
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.09 – 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0.11 – 0.25 0.21 0.17 0.11 0.15 0 0 0 0.45 0.38 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0.22 – 0.71 0.57 0.63 0.6 0.54 0 0 0 0.52 0.42 – 0.25 0.26 0.1 0.19 0.22 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 9 23 19 13 4 – 8 2 4 4 8 28 32 32 0 2 – 2 0 6 0 0 0
Bud 7 2 2 4 3 1 – 4 2 3 2 2 4 4 3 0 0 – 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 2 2 0 0 1 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 103 41 204 135 87 27 – 1 16 11 14 24 211 211 203 2 6 – 70 27 195 14 30 34
Cytoplasm 42 26 26 51 59 91 – 103 173 165 197 205 24 24 18 8 8 – 30 19 6 6 2 2
Endoplasmic Reticulum 96 63 61 92 171 119 – 54 60 49 29 46 55 57 24 3 3 – 95 55 55 16 29 26
Endosome 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 2 0 2 1 4 1 – 2 2 4 1 6 3 3 2 2 0 – 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 0 – 5 6 9 6 14 0 0 0 3 5 – 3 1 3 2 1 0
Nucleus 5 2 1 1 12 34 – 194 133 107 60 85 2 2 4 29 21 – 1 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 3 1 1 1 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 – 3 2 3 0 1 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 – 1 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 5 1 2 5 38 67 – 557 362 388 323 314 9 7 9 33 23 – 70 38 31 10 19 14
Unique Cell Count 221 122 278 253 333 307 790 634 619 534 578 273 273 252 64 55 278 149 305 54 89 82
Labelled Cell Count 275 144 322 308 387 345 935 759 744 637 705 336 340 296 80 68 278 149 305 54 89 82


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.0 32.1 34.6 38.3 43.4 42.3 – 37.1 36.3 33.8 31.6 31.7 22.8 22.8 18.6 19.5 23.2 – 37.0 32.8 33.8
Std Deviation (1e-4) 8.3 9.2 9.3 10.8 12.1 13.6 – 14.4 14.7 13.7 13.9 12.8 5.2 5.2 3.7 8.0 8.5 – 13.3 9.4 9.6
Intensity Change (Log2) – – – 0.15 0.33 0.29 – 0.1 0.07 -0.04 -0.13 -0.13 -0.61 -0.61 -0.9 -0.83 -0.58 – 0.09 -0.08 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.3 -2.3 -4.0 – -6.2 -6.8 -6.2 -5.7 -5.1 0.8 1.4 1.7 0 0 –
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery -4.8 -11.6 -16.0 – -26.7 -23.0 -23.2 -21.6 -21.5 1.1 1.1 2.0 -10.4 -8.8 –
Cytoplasm 3.5 3.0 6.1 – 1.6 6.0 5.9 8.3 8.1 -0.2 -0.2 -0.9 0.8 1.2 –
Endoplasmic Reticulum 3.7 7.5 4.4 – -7.0 -5.1 -5.9 -7.1 -5.8 -0.5 -0.3 -3.9 -3.2 -2.8 –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Nucleus 0 2.8 5.5 – 9.0 8.1 7.2 5.6 6.5 0 0 0 11.5 0 –
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 5.3 7.9 – 20.0 16.0 17.3 16.5 15.2 2.2 0 2.3 12.1 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 34.518 49.6368 44.4674 44.88 42.6583 43.3596 33.9459 48.4913 42.7975 40.6583 44.3325 42.6415 30.6745 44.337 38.4065 33.0976 34.6557 41.0549
Actin 0 0 0 0 0.0001 0 0.0001 0 0 0 0 0 0.0069 0.0001 0.0062 0 0 0
Bud 0.0001 0 0.0001 0.0001 0.0002 0 0.0002 0 0.0001 0 0 0 0.0005 0.0002 0.0036 0.0002 0.0001 0.0001
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002 0.0004 0.0002 0 0 0.0001 0.0005 0.0001 0.0006 0.0001 0.0001 0.0007
Bud Periphery 0.0008 0.0003 0.0008 0.0011 0.0027 0.0007 0.0017 0.0004 0.0007 0.0004 0.0005 0.0006 0.0019 0.0008 0.0061 0.0047 0.0008 0.001
Bud Site 0.0002 0.0004 0.0002 0.0001 0.0002 0 0.0004 0.0001 0.0005 0 0 0 0.0012 0.0008 0.0064 0.0001 0.0002 0.0001
Cell Periphery 0.6135 0.5789 0.606 0.5694 0.5644 0.5899 0.5414 0.5553 0.5581 0.5599 0.5439 0.5251 0.4515 0.4982 0.4611 0.5134 0.5275 0.4797
Cytoplasm 0.0062 0.0034 0.0066 0.0015 0.0013 0.0031 0.0015 0.0005 0.0012 0.0003 0.0002 0.0003 0.0031 0.0011 0.0219 0.0004 0.0008 0.0008
Cytoplasmic Foci 0.0057 0 0 0 0 0 0.0002 0 0.0005 0 0 0 0.0053 0.0005 0.0003 0 0 0.0001
Eisosomes 0.0002 0.0001 0 0 0 0 0.0001 0.0003 0.0001 0 0.0002 0 0.0015 0.0001 0.0002 0 0 0.0005
Endoplasmic Reticulum 0.014 0.0102 0.0158 0.0056 0.0077 0.0075 0.009 0.0086 0.0119 0.005 0.0075 0.0036 0.0036 0.0023 0.0222 0.0054 0.0012 0.0067
Endosome 0.0003 0.0001 0.0001 0.0001 0.0002 0 0.0014 0.0001 0.0004 0.0001 0.0001 0.0002 0.0095 0.0013 0.0012 0.0002 0.0002 0.0018
Golgi 0.0001 0 0 0 0 0 0.0003 0.0001 0.0002 0 0 0 0.002 0.0002 0.0005 0 0 0.0005
Lipid Particles 0.0112 0.0105 0.0111 0.0037 0.0051 0.0067 0.0242 0.0081 0.0252 0.0072 0.0066 0.01 0.0482 0.0313 0.0489 0.0083 0.0064 0.0329
Mitochondria 0 0 0 0 0 0 0.0028 0 0.0003 0 0 0 0.0108 0.0002 0.0005 0.0001 0 0.0004
None 0.0001 0 0 0 0 0 0.0001 0 0.0005 0 0 0 0.0066 0 0.0003 0 0 0
Nuclear Periphery 0.0037 0.0031 0.0033 0.0013 0.0045 0.0016 0.0015 0.002 0.002 0.0009 0.0012 0.0008 0.0014 0.0011 0.0038 0.0013 0.0003 0.0019
Nucleolus 0.0002 0.0002 0.0003 0.0002 0.0002 0.0001 0.0009 0.0003 0.0024 0.0002 0.0003 0.0003 0.002 0.001 0.0014 0.0004 0.0015 0.0004
Nucleus 0.0002 0.0002 0.0002 0.0003 0.0002 0.0002 0.0005 0.0002 0.0012 0.0002 0.0002 0.0002 0.0011 0.0005 0.0023 0.0005 0.0007 0.0004
Peroxisomes 0.0007 0 0 0 0 0 0.0001 0 0 0 0 0 0.0035 0 0.0001 0 0 0
Punctate Nuclear 0.0006 0 0 0 0 0 0 0 0.0004 0 0 0 0.0003 0 0.0001 0 0 0
Vacuole 0.3405 0.3915 0.3539 0.4144 0.4115 0.3886 0.4063 0.4216 0.3904 0.4223 0.4363 0.456 0.4272 0.4494 0.4042 0.459 0.4578 0.466
Vacuole Periphery 0.0017 0.001 0.0014 0.002 0.0016 0.0011 0.0071 0.002 0.0038 0.0033 0.0028 0.0027 0.0112 0.0106 0.0083 0.0058 0.0022 0.0059

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 257.7754 168.6113 113.4393 141.7208 134.0944 191.0252 159.1172 146.707 155.5623 171.7758
Translational Efficiency 0.6043 0.6196 0.7885 0.6953 0.6719 0.7864 0.6203 0.6092 0.6393 0.5374

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘