Standard name
Description Myo-inositol transporter; member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress; ITR1 has a paralog, ITR2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.07 0.08 0.08 0 0 0 0 0 0 0 0.1 0.12 0.13 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.47 0.34 0.73 0.53 0.26 0.09 0 0 0 0 0 0.77 0.77 0.81 0 0.11 0.25 0.19 0.64 0.26 0.35 0.41
Cytoplasm 0.19 0.21 0.09 0.2 0.18 0.3 0.13 0.27 0.27 0.37 0.35 0.09 0.09 0.07 0.12 0.15 0.11 0.13 0 0.12 0 0
Endoplasmic Reticulum 0.43 0.52 0.22 0.36 0.51 0.39 0.07 0.09 0.08 0.05 0.08 0.2 0.21 0.1 0.05 0.05 0.34 0.38 0.18 0.3 0.34 0.32
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0.11 0.25 0.21 0.17 0.11 0.15 0 0 0 0.45 0.38 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0.22 0.71 0.57 0.63 0.6 0.54 0 0 0 0.52 0.42 0.25 0.26 0.1 0.19 0.22 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 9 23 19 13 4 8 2 4 4 8 28 32 32 0 2 2 0 6 0 0 0
Bud 7 2 2 4 3 1 4 2 3 2 2 4 4 3 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 103 41 204 135 87 27 1 16 11 14 24 211 211 203 2 6 70 27 195 14 30 34
Cytoplasm 42 26 26 51 59 91 103 173 165 197 205 24 24 18 8 8 30 19 6 6 2 2
Endoplasmic Reticulum 96 63 61 92 171 119 54 60 49 29 46 55 57 24 3 3 95 55 55 16 29 26
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2 0 2 1 4 1 2 2 4 1 6 3 3 2 2 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 0 5 6 9 6 14 0 0 0 3 5 3 1 3 2 1 0
Nucleus 5 2 1 1 12 34 194 133 107 60 85 2 2 4 29 21 1 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 3 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 3 2 3 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 5 1 2 5 38 67 557 362 388 323 314 9 7 9 33 23 70 38 31 10 19 14
Unique Cell Count 221 122 278 253 333 307 790 634 619 534 578 273 273 252 64 55 278 149 305 54 89 82
Labelled Cell Count 275 144 322 308 387 345 935 759 744 637 705 336 340 296 80 68 278 149 305 54 89 82


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.0 32.1 34.6 38.3 43.4 42.3 37.1 36.3 33.8 31.6 31.7 22.8 22.8 18.6 19.5 23.2 37.0 32.8 33.8
Std Deviation (1e-4) 8.3 9.2 9.3 10.8 12.1 13.6 14.4 14.7 13.7 13.9 12.8 5.2 5.2 3.7 8.0 8.5 13.3 9.4 9.6
Intensity Change (Log2) 0.15 0.33 0.29 0.1 0.07 -0.04 -0.13 -0.13 -0.61 -0.61 -0.9 -0.83 -0.58 0.09 -0.08 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.3 -2.3 -4.0 -6.2 -6.8 -6.2 -5.7 -5.1 0.8 1.4 1.7 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -4.8 -11.6 -16.0 -26.7 -23.0 -23.2 -21.6 -21.5 1.1 1.1 2.0 -10.4 -8.8
Cytoplasm 3.5 3.0 6.1 1.6 6.0 5.9 8.3 8.1 -0.2 -0.2 -0.9 0.8 1.2
Endoplasmic Reticulum 3.7 7.5 4.4 -7.0 -5.1 -5.9 -7.1 -5.8 -0.5 -0.3 -3.9 -3.2 -2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 2.8 5.5 9.0 8.1 7.2 5.6 6.5 0 0 0 11.5 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.3 7.9 20.0 16.0 17.3 16.5 15.2 2.2 0 2.3 12.1 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Itr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available