Standard name
Human Ortholog
Description S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0.07 0.09 0.07 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 0.98 0.99 0.96 0.91 0.83 0.85 0.88 0.89 0.85 1.0 0.99 0.98 1.0 1.0 1.0 0.97 0.95 0.96 0.94 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.13 0.11 0.09 0.07 0.11 0 0 0 0 0.13 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 4 2 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Bud 2 0 1 3 1 0 5 3 0 4 1 4 0 0 0 0 0 0 2 1 0 4 9 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 1 8 4 7 9 24 31 29 21 33 41 5 7 7 3 0 0 0 0 0 0 0 1
Cytoplasm 256 114 272 265 293 342 438 364 275 256 299 353 367 585 475 72 70 53 237 103 261 213 375 393
Endoplasmic Reticulum 1 1 1 2 0 6 30 56 34 27 22 47 1 1 3 2 9 3 1 0 6 0 2 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 1 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0 0 0 1 0 3 2 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 1 0 1 2 1 0 2 1 1 0 3 6 1 0 0 0 0 0 1 1 1
Unique Cell Count 257 115 273 271 295 355 483 437 322 290 337 415 367 589 483 72 70 53 245 109 273 226 396 416
Labelled Cell Count 260 116 282 275 302 360 501 459 343 315 357 451 373 597 491 78 79 56 245 109 273 226 396 416


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 46.1 63.6 56.2 55.1 60.6 61.2 56.9 60.1 56.4 51.3 52.2 50.5 60.8 54.5 49.8 91.8 90.8 85.3 52.9 47.8 48.5
Std Deviation (1e-4) 11.3 17.5 14.5 16.2 18.2 19.0 17.2 17.5 16.6 13.3 14.0 13.8 21.7 17.9 16.5 39.6 37.8 38.2 17.4 16.9 17.2
Intensity Change (Log2) -0.03 0.11 0.12 0.02 0.09 0.0 -0.13 -0.11 -0.16 0.11 -0.04 -0.17 0.71 0.69 0.6 -0.09 -0.24 -0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.2 -0.4 -0.3 1.3 2.4 3.1 2.3 3.4 3.5 -1.4 0 -1.4 0 0 0
Cytoplasm -1.9 -0.5 -2.8 -4.9 -6.9 -6.4 -5.6 -5.5 -6.5 1.2 -0.6 -1.6 0.5 0.5 0.4
Endoplasmic Reticulum 0 0 0 3.9 5.9 5.3 4.9 4.0 5.5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 45.1486 66.7326 55.8375 51.5324 55.4496 62.5455 61.6883 84.9896 76.4297 66.7324 73.4171 79.0435 62.3891 84.8951 71.5425 70.9266 66.6978 81.4619
Actin 0.0065 0.0001 0.0062 0 0.0165 0.0005 0.0018 0.0001 0.0022 0 0.0006 0.0001 0.0024 0.0001 0.0013 0.0002 0.0018 0.0001
Bud 0.0001 0 0.0001 0 0.0004 0.0001 0.0008 0 0.0002 0 0.0002 0.0001 0.0015 0 0.0023 0.0002 0.0004 0.0001
Bud Neck 0.0007 0.0001 0.0002 0.0002 0.0011 0.0004 0.0012 0.0001 0.0006 0.0001 0.0004 0.0004 0.0004 0.0001 0.0002 0.0001 0.0009 0.0003
Bud Periphery 0.0001 0 0.0001 0 0.0005 0.0001 0.0006 0 0.0001 0 0.0001 0 0.0012 0 0.003 0.0001 0.0003 0.0001
Bud Site 0.0003 0 0.0002 0 0.0011 0.0001 0.0026 0 0.0023 0 0.0002 0 0.0005 0 0.0005 0.0005 0.0007 0
Cell Periphery 0.0001 0 0 0 0.0007 0.0003 0.0015 0.0001 0.0002 0 0.0004 0 0.0004 0 0.0005 0.0005 0.0003 0.0001
Cytoplasm 0.9226 0.9987 0.9627 0.9978 0.897 0.9727 0.9072 0.9973 0.9731 0.9972 0.9499 0.9843 0.9337 0.9985 0.944 0.9541 0.9396 0.9868
Cytoplasmic Foci 0.0056 0.0001 0.001 0.0004 0.009 0.0026 0.0115 0.0002 0.0011 0.0001 0.0111 0.0041 0.0072 0.0003 0.0097 0.0101 0.0033 0.0004
Eisosomes 0.0003 0 0.0003 0 0.0003 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0018 0.0001 0.0002 0.0001 0.0032 0.0025 0.0033 0.0002 0.0007 0.0001 0.0029 0.0003 0.0021 0.0001 0.0005 0.0004 0.0009 0.0005
Endosome 0.0077 0 0.0023 0.0001 0.0142 0.0022 0.0075 0 0.0025 0 0.0059 0.0004 0.0072 0 0.0035 0.0008 0.0086 0.0004
Golgi 0.0021 0 0.0013 0 0.0059 0.0004 0.0016 0 0.0006 0 0.0004 0 0.0019 0 0.0015 0.0002 0.0048 0
Lipid Particles 0.0068 0 0.0047 0 0.005 0.0018 0.0075 0 0.0008 0 0.0038 0.0005 0.0053 0 0.0069 0.0089 0.0026 0.0001
Mitochondria 0.0011 0 0.0012 0 0.0032 0.0015 0.0067 0 0.0038 0 0.0008 0.0003 0.0041 0 0.004 0.003 0.0035 0.0002
None 0.0087 0.0001 0.0037 0.0001 0.0089 0.0019 0.0058 0.0001 0.0006 0.0001 0.0088 0.0018 0.0038 0.0001 0.0044 0.0044 0.0097 0.0005
Nuclear Periphery 0.0137 0.0002 0.0035 0.0002 0.0134 0.0027 0.0087 0.0003 0.0009 0.0004 0.0033 0.0011 0.0047 0.0001 0.001 0.0012 0.0025 0.0008
Nucleolus 0.002 0 0.0018 0 0.0032 0.0005 0.0045 0 0.0016 0 0.0003 0.0008 0.0021 0 0.0027 0.0049 0.0016 0.0002
Nucleus 0.0095 0.0006 0.005 0.0009 0.0071 0.0037 0.0083 0.001 0.0035 0.0016 0.003 0.004 0.0075 0.0005 0.0037 0.0029 0.0068 0.007
Peroxisomes 0.0017 0 0.0025 0 0.0007 0.0001 0.002 0 0.0002 0 0.0003 0.0001 0.0012 0 0.0032 0.0005 0.0004 0
Punctate Nuclear 0.0067 0 0.0022 0 0.0037 0.0003 0.0025 0 0.0003 0 0.0012 0.0005 0.0028 0 0.002 0.0009 0.0005 0.0001
Vacuole 0.0014 0.0001 0.0004 0.0001 0.0038 0.0033 0.0109 0.0003 0.0027 0.0003 0.0052 0.001 0.0072 0.0001 0.0034 0.0036 0.0079 0.0019
Vacuole Periphery 0.0007 0 0.0004 0 0.0013 0.0022 0.0034 0 0.0018 0 0.001 0.0004 0.0027 0 0.0016 0.0024 0.0027 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 301.4061 150.3049 178.7059 231.4348 154.2088 210.0559 134.9194 140.1618 225.7264 177.2572
Translational Efficiency 1.4021 1.3886 1.3815 1.5153 1.5015 1.9311 1.3891 1.5443 1.5866 1.69

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1937 220 1403 216 1121 1730 503 999 3058 1950 1906 1215

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3170.33 3103.37 2888.31 2699.77 2953.30 2328.85 2676.48 2871.38 3090.77 2416.23 2832.41 2840.87
Standard Deviation 886.02 905.61 1029.49 932.69 852.31 716.45 844.16 1158.50 880.05 779.72 988.40 1123.60
Intensity Change Log 2 -0.030797 -0.134407 -0.231797 -0.342710 -0.141991 -0.040584 -0.172845 -0.138060 -0.136412

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000043 0.000211 0.000153 0.000861 0.000118 0.000080 0.000336 0.000329 0.000070 0.000095 0.000201 0.000424
Bud Neck 0.000194 0.001397 0.002146 0.005962 0.000576 0.000334 0.002806 0.002530 0.000334 0.000454 0.002320 0.003141
Bud Site 0.001028 0.004804 0.003865 0.017916 0.002351 0.002431 0.008020 0.014627 0.001513 0.002699 0.004962 0.015212
Cell Periphery 0.000710 0.002495 0.001062 0.005367 0.002558 0.000121 0.000783 0.002014 0.001387 0.000389 0.000988 0.002610
Cytoplasm 0.931617* 0.851240* 0.810501* 0.716590* 0.889233* 0.928507* 0.789604* 0.815340* 0.916080* 0.919789* 0.804986* 0.797784*
Cytoplasmic Foci 0.004463 0.021593 0.019038 0.027470 0.009759 0.008088 0.020152 0.019426 0.006404 0.009612 0.019332 0.020856
Eisosomes 0.000000 0.000002 0.000002 0.000007 0.000001 0.000001 0.000003 0.000003 0.000001 0.000001 0.000002 0.000004
Endoplasmic Reticulum 0.000051 0.000117 0.000074 0.000503 0.000149 0.000091 0.000142 0.000065 0.000087 0.000094 0.000092 0.000143
Endosome 0.000088 0.000346 0.000467 0.002312 0.000387 0.000422 0.001070 0.001065 0.000198 0.000413 0.000626 0.001287
Golgi 0.002376 0.004654 0.001972 0.007612 0.011170 0.001063 0.002194 0.002623 0.005600 0.001469 0.002031 0.003510
Lipid Particles 0.001612 0.007275 0.001724 0.012903 0.004857 0.000342 0.004137 0.005884 0.002802 0.001124 0.002361 0.007132
Mitochondria 0.000068 0.000118 0.000207 0.000072 0.000148 0.000062 0.000175 0.000312 0.000097 0.000068 0.000199 0.000270
Mitotic Spindle 0.000352 0.002768 0.005142 0.006425 0.001218 0.001450 0.010122 0.010682 0.000669 0.001599 0.006456 0.009926
None 0.008503 0.005958 0.004811 0.002097 0.005486 0.006452 0.005148 0.003951 0.007397 0.006396 0.004900 0.003622
Nuclear Periphery 0.000053 0.000303 0.000324 0.000330 0.000173 0.000041 0.000317 0.000207 0.000097 0.000071 0.000322 0.000229
Nuclear Periphery Foci 0.002384 0.005879 0.002213 0.010709 0.005734 0.001171 0.005372 0.004326 0.003612 0.001702 0.003046 0.005460
Nucleolus 0.000041 0.000102 0.000151 0.000131 0.000070 0.000061 0.000116 0.000121 0.000051 0.000065 0.000142 0.000123
Nucleus 0.041104 0.069817 0.126980 0.076338 0.048820 0.044034 0.117166 0.076068 0.043932 0.046943 0.124390 0.076116
Peroxisomes 0.000009 0.000031 0.000025 0.000158 0.000013 0.000026 0.000247 0.000214 0.000010 0.000027 0.000084 0.000204
Vacuole 0.004460 0.016208 0.018000 0.103594 0.012060 0.004873 0.031719 0.038089 0.007246 0.006152 0.021620 0.049734
Vacuole Periphery 0.000847 0.004682 0.001144 0.002642 0.005118 0.000349 0.000370 0.002123 0.002412 0.000838 0.000939 0.002215

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.89 -9.51 -5.90 -4.37 -4.57 2.25 -7.71 -6.47 -7.54 1.07 -2.55 -11.60 -9.69 -8.78 -4.13
Bud Neck -4.25 -13.16 -7.60 -5.40 -4.19 3.42 -8.92 -12.57 -13.99 2.18 -3.15 -15.65 -15.14 -14.18 -1.59
Bud Site -5.35 -13.71 -5.77 -4.44 -4.38 -0.07 -4.19 -7.32 -7.33 -3.23 -3.86 -10.05 -9.32 -8.51 -6.26
Cell Periphery -2.68 -3.80 -5.12 -1.54 -4.26 8.10 5.03 3.26 -10.97 -3.38 7.07 1.63 -2.53 -9.26 -4.43
Cytoplasm 8.80 30.74 17.00 9.65 7.26 -7.05 15.72 18.65 25.64 0.14 0.20 32.32 28.46 28.04 6.11
Cytoplasmic Foci -7.23 -16.02 -8.09 -3.01 -3.27 1.59 -9.07 -10.11 -10.87 -2.61 -5.12 -17.62 -13.56 -11.71 -4.92
Eisosomes -5.47 -12.74 -10.07 -7.90 -7.32 3.26 -7.97 -11.75 -13.86 0.09 -1.44 -13.94 -16.53 -16.17 -6.89
Endoplasmic Reticulum -2.27 -3.14 -3.91 -2.66 -3.32 1.55 -1.00 3.01 0.59 4.65 -0.32 -2.43 -2.01 -1.06 -0.27
Endosome -4.52 -8.88 -5.58 -4.62 -3.84 -0.35 -5.08 -2.24 -1.80 1.97 -2.25 -9.18 -5.10 -3.17 -1.10
Golgi -2.36 1.24 -2.71 -0.13 -3.10 8.25 7.37 7.69 -0.88 1.53 8.26 7.30 6.23 -1.43 0.26
Lipid Particles -2.05 -2.78 -6.82 -2.12 -6.32 5.68 -0.44 -3.46 -8.34 -3.17 3.52 -1.26 -7.44 -9.12 -7.10
Mitochondria -1.06 -9.77 -1.41 0.76 8.53 3.07 -0.81 -0.66 -1.88 -0.30 2.05 -7.74 -1.34 -1.81 1.04
Mitotic Spindle -2.67 -8.31 -2.17 -1.49 -0.38 -0.55 -3.68 -6.76 -6.65 -3.28 -2.42 -8.31 -7.28 -6.83 -4.32
None 2.84 7.66 12.14 3.95 10.43 -1.55 1.78 4.41 6.08 1.50 2.29 7.58 10.70 7.20 5.04
Nuclear Periphery -2.64 -15.50 -10.11 -1.47 0.41 4.86 -7.70 -5.49 -13.14 3.32 1.50 -16.14 -12.24 -12.85 3.36
Nuclear Periphery Foci -4.50 -1.21 -7.42 -3.53 -6.98 8.32 -1.38 -0.25 -8.43 1.15 6.35 -0.82 -5.89 -9.82 -5.17
Nucleolus -2.72 -13.08 -7.36 -0.99 2.18 0.47 -5.86 -4.43 -6.21 1.91 -1.80 -13.61 -9.35 -6.38 3.98
Nucleus -5.98 -27.89 -7.19 -1.77 5.53 1.30 -13.62 -10.48 -12.96 6.61 -2.61 -30.30 -15.06 -12.95 11.26
Peroxisomes -3.70 -10.11 -3.05 -2.63 -2.60 -2.38 -1.90 -2.99 -2.78 -0.32 -3.45 -2.66 -3.50 -3.20 -2.22
Vacuole -4.83 -12.76 -10.95 -9.38 -8.65 4.04 -9.70 -15.45 -17.26 -2.42 -0.64 -15.36 -19.89 -19.61 -10.87
Vacuole Periphery -0.94 -0.49 -1.54 0.73 -1.08 4.34 4.35 3.57 -1.41 -1.44 2.77 3.20 2.45 -0.53 -0.48