Standard name
Description Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.06 0.09 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.96 0.97 0.95 0.93 0.86 0.86 0.75 0.7 0.7 0.73 0.99 0.99 0.99 0.94 0.93 0.96 0.97 0.92 0.89 0.83 0.86 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.1 0.14 0.31 0.32 0.34 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0.08 0.05 0.11 0.08 0.09 0 0 0 0 0.08 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 3 1 0 0 1 3 0 0 0 0 0 0 0 2 0 0 5 4
Bud 0 3 1 1 0 2 1 2 5 9 7 6 0 0 0 0 0 0 0 3 0 1 9 11
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 5 5 5 9 5 8 7 8 11 15 3 3 1 10 18 13 18 0 0 0 0 0 0
Cytoplasm 239 408 258 221 332 267 294 262 269 247 225 232 286 458 553 285 209 190 229 400 245 186 500 350
Endoplasmic Reticulum 1 2 1 1 1 1 9 1 4 1 3 6 2 5 3 8 6 6 0 1 2 8 16 8
Endosome 0 1 1 1 2 0 0 0 0 0 2 1 0 1 2 1 0 0 1 4 2 1 6 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 2 0 0 3 0
Mitochondria 1 1 3 4 11 21 33 42 112 112 109 83 1 0 0 1 1 1 0 3 8 4 9 6
Nucleus 1 5 1 0 0 4 6 4 1 1 5 5 2 1 0 3 2 3 0 2 1 0 0 1
Nuclear Periphery 0 0 0 0 0 2 0 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
SpindlePole 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 2 0 2 1
Vac/Vac Membrane 0 8 5 2 16 8 7 23 18 39 26 28 0 1 0 12 17 2 0 7 3 11 13 8
Unique Cell Count 241 419 268 229 351 287 341 306 357 354 320 318 290 464 556 303 225 198 238 434 275 224 583 407
Labelled Cell Count 245 434 275 236 372 310 362 343 418 422 396 368 296 467 568 329 249 221 238 434 275 224 583 407


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 7.3 6.6 6.4 7.2 6.0 6.0 6.2 5.3 5.3 5.3 5.7 7.4 7.9 7.8 9.3 10.1 9.6 6.7 6.8 7.5
Std Deviation (1e-4) 0.8 0.9 1.0 0.6 1.1 1.3 1.2 1.1 1.0 1.1 1.1 1.0 1.1 1.2 1.2 1.3 1.3 1.1 1.5 1.3 1.6
Intensity Change (Log2) -0.05 0.12 -0.13 -0.15 -0.09 -0.33 -0.31 -0.32 -0.22 0.16 0.25 0.23 0.49 0.61 0.54 0.02 0.04 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.6 0 0.4 0.4 0.3 1.0 1.9 0 0 0 0 2.5 2.3 3.6
Cytoplasm 0.1 -1.0 -1.7 -4.2 -4.4 -7.1 -8.4 -8.2 -7.6 1.8 2.2 3.4 -1.2 -1.7 -0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.7 3.6 4.4 5.6 9.7 9.7 10.1 8.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.8 0.7 0.2 3.1 2.1 4.4 3.4 3.6 0 0 0 1.5 3.0 0