Standard name | |
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Human Ortholog | |
Description | Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 0 | 2 | 4 | 3 | 5 | 2 | 5 | 0 | 2 | 3 | 1 | 4 | 3 | 4 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 12 | 23 |
Bud | 13 | 0 | 7 | 11 | 48 | 40 | 42 | 66 | 40 | 36 | 24 | 31 | 5 | 5 | 12 | 5 | 0 | 0 | 100 | 13 | 30 | 9 | 6 | 13 |
Bud Neck | 59 | 1 | 20 | 21 | 76 | 38 | 71 | 97 | 42 | 49 | 28 | 32 | 13 | 19 | 27 | 20 | 7 | 11 | 131 | 8 | 33 | 12 | 12 | 22 |
Bud Site | 41 | 2 | 10 | 24 | 50 | 35 | 39 | 28 | 22 | 17 | 8 | 23 | 10 | 21 | 19 | 9 | 1 | 4 | – | – | – | – | – | – |
Cell Periphery | 28 | 0 | 0 | 6 | 6 | 9 | 7 | 6 | 12 | 11 | 12 | 22 | 6 | 6 | 14 | 12 | 3 | 7 | 1 | 0 | 0 | 0 | 2 | 9 |
Cytoplasm | 158 | 11 | 50 | 71 | 107 | 96 | 217 | 254 | 145 | 207 | 149 | 180 | 35 | 49 | 54 | 32 | 24 | 43 | 102 | 23 | 29 | 38 | 124 | 98 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 1 | 1 | 3 |
Endosome | 6 | 0 | 2 | 1 | 6 | 4 | 5 | 5 | 0 | 0 | 0 | 0 | 2 | 6 | 2 | 1 | 1 | 3 | 3 | 0 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 1 | 1 | 8 | 20 | 15 | 27 | 43 | 79 | 67 | 103 | 67 | 103 | 1 | 2 | 4 | 8 | 0 | 5 | 1 | 0 | 1 | 2 | 1 | 5 |
Nucleus | 19 | 0 | 6 | 13 | 18 | 25 | 24 | 39 | 12 | 20 | 9 | 6 | 1 | 7 | 11 | 0 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 42 | 1 | 12 | 5 | 27 | 26 | 33 | 70 | 28 | 40 | 16 | 21 | 8 | 13 | 12 | 15 | 5 | 12 | 3 | 0 | 3 | 0 | 0 | 0 |
Peroxisomes | 3 | 1 | 2 | 3 | 13 | 7 | 4 | 4 | 4 | 6 | 2 | 2 | 8 | 8 | 5 | 2 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 36 | 0 | 4 | 15 | 45 | 43 | 65 | 64 | 29 | 31 | 15 | 25 | 9 | 16 | 13 | 6 | 3 | 4 | 21 | 1 | 7 | 0 | 0 | 0 |
Vac/Vac Membrane | 7 | 1 | 0 | 1 | 3 | 4 | 2 | 10 | 2 | 1 | 5 | 5 | 1 | 0 | 0 | 5 | 1 | 3 | 7 | 1 | 1 | 1 | 3 | 5 |
Unique Cell Count | 322 | 18 | 96 | 150 | 309 | 246 | 421 | 534 | 275 | 375 | 250 | 316 | 75 | 120 | 132 | 83 | 37 | 81 | 383 | 54 | 115 | 69 | 172 | 192 |
Labelled Cell Count | 418 | 19 | 123 | 195 | 417 | 359 | 556 | 730 | 404 | 523 | 339 | 451 | 104 | 155 | 177 | 119 | 46 | 100 | 383 | 54 | 115 | 69 | 172 | 192 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.4 | 6.1 | 5.2 | 5.1 | 5.5 | 5.0 | 4.7 | 4.7 | 4.2 | 4.1 | 4.0 | 3.7 | 8.0 | 8.1 | 7.2 | 12.1 | 11.4 | 14.6 | 6.8 | 7.2 | 6.6 |
Std Deviation (1e-4) | 2.9 | 1.4 | 1.3 | 1.2 | 1.4 | 1.3 | 1.4 | 1.5 | 1.1 | 1.4 | 1.3 | 1.3 | 2.4 | 2.4 | 2.2 | 3.0 | 4.7 | 5.8 | 1.2 | 7.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.08 | -0.05 | -0.15 | -0.14 | -0.3 | -0.34 | -0.37 | -0.47 | 0.63 | 0.66 | 0.49 | 1.22 | 1.14 | 1.5 | 0.39 | 0.47 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2.1 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | -1.0 | 0.5 | -0.3 | 0 | 0 |
Bud Neck | -1.4 | 0.8 | -1.2 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -0.9 | -0.1 | 0.5 | -0.2 | -1.3 |
Bud Site | 1.2 | 1.4 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 1.5 | 0.9 | 0.1 | 0 | -1.3 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.9 | 0 | 0 |
Cytoplasm | -0.7 | -3.1 | -2.2 | -0.1 | -0.8 | 0.1 | 0.5 | 1.3 | 0.8 | -0.7 | -1.6 | -1.7 | -1.8 | 1.3 | 0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.2 | -1.3 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | 0.3 | 0 | -0.5 |
Nucleus | 0.7 | -0.2 | 1.1 | -0.2 | 0.4 | 0 | -0.4 | 0 | 0 | 0 | -0.1 | 0.6 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.8 | -1.1 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | -0.4 | -0.8 | 1.0 | 0 | 0.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.7 | 2.7 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.3 | 1.6 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3191 | 5.053 | 5.2925 | 5.0205 | 4.2327 | 4.4829 | 4.5631 | 6.928 | 8.1346 | 6.9925 | 6.1684 | 6.6168 | 2.4267 | 4.7324 | 5.2301 | 5.104 | 4.9833 | 3.6826 |
Actin | 0.0053 | 0.0008 | 0.001 | 0.0001 | 0.0002 | 0.0103 | 0.0731 | 0.0173 | 0.032 | 0.0529 | 0.0274 | 0.015 | 0.0102 | 0.0015 | 0.0067 | 0.0116 | 0.0002 | 0.0063 |
Bud | 0.0289 | 0.0257 | 0.0083 | 0.0002 | 0.0019 | 0.0207 | 0.017 | 0.033 | 0.0092 | 0.0006 | 0.0021 | 0.0228 | 0.0238 | 0.0407 | 0.0075 | 0.0007 | 0.0012 | 0.0381 |
Bud Neck | 0.0457 | 0.0409 | 0.6618 | 0.9053 | 0.9193 | 0.4683 | 0.0382 | 0.0244 | 0.5757 | 0.8695 | 0.4883 | 0.4191 | 0.0571 | 0.0287 | 0.6881 | 0.953 | 0.9163 | 0.4249 |
Bud Periphery | 0.0182 | 0.0291 | 0.0087 | 0.0002 | 0.0023 | 0.0207 | 0.0119 | 0.0262 | 0.0052 | 0.0008 | 0.0038 | 0.033 | 0.0176 | 0.0184 | 0.0074 | 0.0008 | 0.0017 | 0.0411 |
Bud Site | 0.4261 | 0.7021 | 0.2879 | 0.0048 | 0.0028 | 0.0433 | 0.2494 | 0.6486 | 0.2874 | 0.0025 | 0.018 | 0.0226 | 0.4617 | 0.6388 | 0.1984 | 0.0037 | 0.0255 | 0.0278 |
Cell Periphery | 0.0087 | 0.0055 | 0.0033 | 0.0009 | 0.0014 | 0.0052 | 0.0068 | 0.0089 | 0.0042 | 0.001 | 0.0017 | 0.002 | 0.0067 | 0.0083 | 0.0031 | 0.0006 | 0.0014 | 0.0053 |
Cytoplasm | 0.2281 | 0.0855 | 0.0003 | 0.0439 | 0.0004 | 0.3054 | 0.2412 | 0.1632 | 0.0098 | 0.0011 | 0.0197 | 0.3886 | 0.1697 | 0.073 | 0.0006 | 0.003 | 0.0001 | 0.3318 |
Cytoplasmic Foci | 0.1161 | 0.027 | 0.0076 | 0.0035 | 0.0131 | 0.074 | 0.1224 | 0.0316 | 0.0406 | 0.0279 | 0.0651 | 0.0323 | 0.0892 | 0.0436 | 0.0239 | 0.0023 | 0.0246 | 0.0526 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0011 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0054 | 0.0003 | 0 | 0.0003 | 0 | 0.0012 | 0.0149 | 0.0008 | 0.0001 | 0.0012 | 0.0054 | 0.0013 | 0.0048 | 0.0006 | 0.0001 | 0.0004 | 0 | 0.0017 |
Endosome | 0.0013 | 0.0008 | 0.0001 | 0.0012 | 0.0005 | 0.0015 | 0.0634 | 0.001 | 0.0025 | 0.008 | 0.1115 | 0.0038 | 0.0148 | 0.0011 | 0.0016 | 0.0013 | 0.0002 | 0.0043 |
Golgi | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0006 | 0.0013 | 0.0319 | 0.0011 | 0.0039 | 0.015 | 0.0689 | 0.0033 | 0.0049 | 0.0003 | 0.0016 | 0.0006 | 0.0003 | 0.0015 |
Lipid Particles | 0.0064 | 0.0005 | 0.0005 | 0.0003 | 0.0038 | 0.0028 | 0.0439 | 0.0009 | 0.0044 | 0.0032 | 0.0244 | 0.0068 | 0.0115 | 0.0007 | 0.0065 | 0.0002 | 0.0152 | 0.0042 |
Mitochondria | 0.0016 | 0.0034 | 0.0002 | 0.0001 | 0.0006 | 0.0005 | 0.0073 | 0.0012 | 0.0042 | 0.0008 | 0.0513 | 0.0045 | 0.0127 | 0.0014 | 0.0066 | 0.0003 | 0.001 | 0.0049 |
None | 0.071 | 0.0024 | 0.0001 | 0.0002 | 0 | 0.0088 | 0.0112 | 0.002 | 0.0003 | 0.0002 | 0.0087 | 0.0157 | 0.0369 | 0.0162 | 0.0004 | 0.0013 | 0.0001 | 0.0241 |
Nuclear Periphery | 0.0005 | 0.0014 | 0 | 0 | 0 | 0.0003 | 0.0207 | 0.0011 | 0.0001 | 0.0002 | 0.0088 | 0.0003 | 0.0017 | 0.0007 | 0.0003 | 0.0005 | 0 | 0.0007 |
Nucleolus | 0.013 | 0.0165 | 0.0122 | 0.034 | 0.0423 | 0.0065 | 0.0045 | 0.0109 | 0.0094 | 0.0037 | 0.0113 | 0.0214 | 0.0272 | 0.0307 | 0.0219 | 0.0144 | 0.007 | 0.0047 |
Nucleus | 0.0026 | 0.0378 | 0.0033 | 0.0029 | 0.0042 | 0.0032 | 0.0041 | 0.0069 | 0.0022 | 0.0008 | 0.0065 | 0.0013 | 0.0044 | 0.037 | 0.0033 | 0.0025 | 0.0018 | 0.0048 |
Peroxisomes | 0.0056 | 0.0007 | 0.0004 | 0.0001 | 0.0037 | 0.003 | 0.0141 | 0.0011 | 0.0049 | 0.0063 | 0.0111 | 0.0026 | 0.0181 | 0.0031 | 0.0139 | 0.0007 | 0.0017 | 0.0026 |
Punctate Nuclear | 0.0088 | 0.0092 | 0.0024 | 0.0006 | 0.0011 | 0.0198 | 0.0112 | 0.0029 | 0.001 | 0.0033 | 0.0488 | 0.0015 | 0.0155 | 0.0092 | 0.0061 | 0.002 | 0.0009 | 0.0076 |
Vacuole | 0.0052 | 0.0085 | 0.0016 | 0.0009 | 0.0015 | 0.003 | 0.0089 | 0.0159 | 0.0023 | 0.0005 | 0.0113 | 0.0018 | 0.0104 | 0.043 | 0.0018 | 0.0002 | 0.0006 | 0.0101 |
Vacuole Periphery | 0.0003 | 0.0016 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0033 | 0.0008 | 0.0002 | 0.0001 | 0.0046 | 0.0004 | 0.001 | 0.0026 | 0.0002 | 0 | 0.0001 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 78.5158 | 28.7657 | 19.6378 | 40.0398 | 71.5515 | 80.402 | 29.0123 | 21.986 | 32.3802 | 77.2415 |
Translational Efficiency | 1.5588 | 1.5435 | 0.9404 | 1.0186 | 1.2272 | 1.569 | 1.1018 | 0.8093 | 0.8441 | 1.0614 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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