Standard name
Human Ortholog
Description Acetylated cis-Golgi protein, homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; required for unconventional protein secretion (UPS) of Acb1p in response to starvation; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.17 0.4 0.57 0.29 0.32 0.23 0.18 0.2 0.17 0.17 0.11 0.2 0.46 0.43 0.3 0.37 0.32 0.44 0.11 0.2 0.11 0.16 0.18 0.14
Bud 0.07 0 0 0.05 0.08 0.07 0.09 0 0.11 0 0.1 0.08 0.05 0.05 0.06 0.05 0.07 0.11 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0.09 0.08 0.07 0.06 0.05 0.11 0 0 0 0 0.11 0.16 0.19 0.1 0.1 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.2 0.19 0.1 0 0.08 0.05 0.09 0 0 0.06 0 0.2 0.15 0.12 0 0 0.06 0 0 0 0 0.11 0.14
Endosome 0.13 0 0.08 0.06 0.09 0.08 0.05 0.07 0 0.07 0 0.09 0.06 0.08 0.09 0 0 0 0.09 0 0.13 0.09 0.05 0
Golgi 0.4 0.73 0.58 0.63 0.73 0.54 0.23 0.33 0.15 0.16 0.12 0.28 0.56 0.61 0.61 0.74 0.76 0.72 0.71 0.69 0.63 0.65 0.58 0.55
Mitochondria 0.8 0.33 0.14 0.49 0.32 0.6 0.77 0.6 0.87 0.79 0.88 0.72 0 0 0 0.06 0.12 0.09 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.12 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 23 55 64 30 71 49 46 51 34 35 33 68 90 97 76 111 107 127 16 33 19 25 37 29
Bud 9 3 4 5 19 15 24 11 22 8 30 27 10 11 14 16 24 33 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 2 1 0 0 0 0 0 0 0 1
Cell Periphery 0 2 1 3 1 2 0 0 2 2 1 4 1 5 6 6 15 10 0 0 0 0 0 0
Cytoplasm 5 10 10 8 16 12 14 28 7 8 13 7 21 37 47 29 35 25 2 3 2 3 6 8
Endoplasmic Reticulum 6 27 22 11 10 17 12 23 4 5 18 10 39 34 31 12 15 17 2 3 4 6 23 29
Endosome 17 2 9 6 20 17 13 17 4 15 10 31 11 18 22 7 4 4 14 3 22 14 9 8
Golgi 54 101 66 66 164 116 59 85 31 33 35 96 109 139 153 222 258 208 113 114 110 102 119 112
Mitochondria 108 45 16 51 72 129 200 152 176 160 261 247 7 6 1 17 42 26 3 1 9 2 2 5
Nucleus 1 0 1 1 0 0 0 1 0 0 1 2 0 0 0 0 1 1 1 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 1 2 0 2 2 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 1 1 1 0 1 0 2 7 7 13 3 7 1 0 0 4 2 2 0 0 0 0 0 0
Peroxisomes 1 0 1 0 2 1 0 4 6 1 6 8 0 1 1 0 0 0 1 1 1 1 1 0
SpindlePole 0 1 2 1 7 2 6 6 6 6 3 11 7 8 3 13 4 3 0 0 0 0 0 0
Vac/Vac Membrane 2 2 2 2 2 3 1 3 1 8 4 7 2 3 5 51 42 13 0 2 1 0 0 1
Unique Cell Count 135 138 113 105 225 214 260 255 202 203 297 343 195 228 250 302 339 288 160 167 174 158 206 203
Labelled Cell Count 228 249 199 184 385 363 379 392 302 297 420 526 298 359 359 488 549 470 160 167 174 158 206 203


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 15.7 11.5 10.7 11.1 10.0 9.3 9.7 8.8 8.7 8.7 9.0 12.8 13.5 13.7 20.3 20.4 19.2 12.2 13.1 14.3
Std Deviation (1e-4) 1.9 2.3 2.0 1.8 1.6 1.7 1.9 1.8 1.9 1.6 1.9 1.6 2.2 1.9 2.2 4.8 5.1 5.0 2.0 2.1 2.4
Intensity Change (Log2) -0.1 -0.06 -0.2 -0.31 -0.24 -0.39 -0.41 -0.4 -0.35 0.16 0.23 0.25 0.82 0.83 0.74 0.08 0.19 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.2 -4.4 -6.1 -7.6 -7.0 -7.3 -7.2 -9.7 -7.5 -1.8 -2.5 -4.8 -3.7 -4.8 -2.3
Bud 0 1.7 1.3 1.9 0 2.3 0 2.2 1.6 0.6 0 0.8 0.7 1.4 2.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -0.6 -1.1 -1.3 0.6 -2.0 -1.8 -1.8 0 0.5 1.9 2.4 0.2 0.5 -0.1
Endoplasmic Reticulum -1.9 -4.5 -3.1 -4.6 -2.8 -5.4 -5.2 -4.1 -6.0 0.1 -1.1 -1.8 -5.1 -5.1 -4.1
Endosome -0.7 0.3 0 -1.1 -0.4 0 -0.2 -2.0 0.3 -0.8 0 0.3 0 0 0
Golgi 0.7 2.7 -0.7 -6.7 -4.5 -7.9 -7.7 -9.8 -5.9 -0.4 0.5 0.5 3.0 3.6 2.7
Mitochondria 5.5 3.5 8.0 11.3 8.1 12.7 11.1 14.2 10.8 -3.4 -4.1 -5.7 -2.9 -0.5 -1.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2.3 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 4.1 3.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.7276 11.1645 10.3744 10.4507 9.6376 10.5666 9.4457 10.704 9.756 9.3168 9.4201 10.0484 8.8656 9.9701 8.9264 8.7558 8.4534 9.112
Actin 0.0732 0.0659 0.0768 0.0046 0.0321 0.056 0.0659 0.0201 0.0777 0.0249 0.048 0.0248 0.0678 0.0169 0.0608 0.0259 0.0035 0.0697
Bud 0.0003 0.0004 0.0003 0 0 0.0001 0.0001 0.0002 0.0005 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0.0001
Bud Neck 0.0005 0.0002 0.0018 0.0002 0.0001 0.0004 0.0015 0.0002 0.0004 0.0002 0.0005 0.0004 0.0003 0.0002 0.0003 0.0002 0.0001 0.0006
Bud Periphery 0.0002 0.0002 0.0003 0 0 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0.0004 0 0.0001 0 0 0.0001
Bud Site 0.0015 0.0014 0.0006 0 0 0.0001 0.0004 0.0021 0.0021 0.0001 0.0001 0.0001 0.0005 0.0013 0.0001 0.0001 0 0.0001
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0048 0.0032 0.002 0.0015 0.001 0.0026 0.0042 0.0095 0.0028 0.0014 0.0018 0.0023 0.0013 0.0057 0.0017 0.0015 0.0012 0.0024
Cytoplasmic Foci 0.0753 0.1082 0.0567 0.0614 0.0627 0.0525 0.0875 0.1335 0.0872 0.097 0.0797 0.0634 0.077 0.1209 0.0769 0.1041 0.0817 0.0639
Eisosomes 0.0001 0 0.0001 0 0 0.0001 0.0002 0 0 0 0 0 0.0001 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0029 0.0004 0.0004 0.0006 0.0003 0.0007 0.0007 0.0005 0.0004 0.0003 0.0004 0.0007 0.0004 0.0003 0.0004 0.0003 0.0003 0.0005
Endosome 0.1032 0.0957 0.0602 0.076 0.0806 0.0628 0.0847 0.1267 0.0773 0.1022 0.0733 0.0797 0.08 0.0971 0.0733 0.11 0.0875 0.0646
Golgi 0.7133 0.7168 0.7687 0.852 0.8198 0.8084 0.7284 0.6878 0.7315 0.762 0.7839 0.8154 0.7561 0.7478 0.7749 0.748 0.8203 0.784
Lipid Particles 0.0083 0.0012 0.0016 0.0011 0.0007 0.0017 0.0067 0.0011 0.0035 0.0028 0.0022 0.001 0.0028 0.005 0.0027 0.0025 0.0009 0.0017
Mitochondria 0.0068 0.0011 0.0031 0.001 0.0009 0.0028 0.0028 0.0014 0.0022 0.0013 0.0019 0.0073 0.0048 0.0008 0.002 0.0008 0.0013 0.0029
None 0.0005 0 0.0007 0 0 0.0006 0.0044 0 0 0 0 0 0.0003 0 0 0 0 0.0001
Nuclear Periphery 0.0007 0 0.0001 0 0 0.0013 0.0004 0.0002 0 0 0 0.0001 0.0003 0 0 0 0 0.0007
Nucleolus 0.0001 0 0.0009 0 0 0.0002 0.0004 0 0 0 0 0 0.0001 0 0 0 0 0
Nucleus 0.0001 0 0.0004 0 0 0.0003 0.0008 0.0001 0 0 0 0 0.0002 0 0 0 0 0.0001
Peroxisomes 0.0053 0.0047 0.0243 0.001 0.0012 0.0071 0.0077 0.0106 0.0135 0.0071 0.0072 0.0034 0.0062 0.0034 0.0063 0.006 0.0021 0.0076
Punctate Nuclear 0.0006 0.0001 0.0002 0 0 0.0017 0.0026 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0 0 0 0.0002
Vacuole 0.0011 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0042 0.0001 0.0001 0.0002 0.0004 0.0005 0.0001 0.0001 0.0002 0.0004 0.0002
Vacuole Periphery 0.001 0.0003 0.0003 0.0003 0.0004 0.0004 0.0003 0.0017 0.0003 0.0003 0.0003 0.0006 0.0006 0.0002 0.0003 0.0004 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.8937 34.3777 35.3212 57.1842 54.9744 36.4641 44.1175 45.1157 61.8581 64.9534
Translational Efficiency 1.1761 1.1136 1.2206 1.0482 1.0913 1.4117 0.9697 1.0957 1.0975 0.9076

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
21 1299 232 2030 1313 1799 795 1646 1334 3098 1027 3676

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1276.83 1210.06 1470.21 1505.33 1148.44 1211.22 1557.77 1485.65 1150.46 1210.73 1537.99 1496.52
Standard Deviation 218.69 183.79 195.14 218.83 173.73 163.38 201.65 233.97 175.26 172.23 203.52 225.95
Intensity Change Log 2 -0.077488 0.203456 0.237513 0.076785 0.439807 0.371419 -0.002375 0.320210 0.302473

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.027988 0.060320 0.038450 0.079031 0.046327 0.075366 0.079632 0.088302 0.046038 0.069058 0.070329 0.083182
Bud Neck 0.003805 0.000161 0.000916 0.000410 0.000188 0.000163 0.000708 0.000597 0.000245 0.000162 0.000755 0.000494
Bud Site 0.008123 0.001254 0.003237 0.004481 0.001243 0.002041 0.002290 0.007317 0.001352 0.001711 0.002504 0.005750
Cell Periphery 0.001194 0.000198 0.000313 0.000707 0.000203 0.000172 0.000260 0.000594 0.000219 0.000183 0.000272 0.000656
Cytoplasm 0.087299 0.000735 0.000296 0.001625 0.000551 0.000929 0.000429 0.003114 0.001917 0.000848 0.000399 0.002292
Cytoplasmic Foci 0.099879 0.185392* 0.133600 0.101444 0.210483* 0.204891* 0.096103 0.132397 0.208742* 0.196715* 0.104574 0.115304
Eisosomes 0.000608 0.000388 0.000278 0.000620 0.000293 0.000416 0.000366 0.000378 0.000298 0.000404 0.000346 0.000512
Endoplasmic Reticulum 0.070156 0.004923 0.010314 0.008188 0.004352 0.004111 0.007690 0.003070 0.005388 0.004451 0.008282 0.005896
Endosome 0.124881 0.171634 0.206729* 0.141612 0.151985 0.156266 0.146919 0.152880 0.151558 0.162710 0.160430 0.146657
Golgi 0.272230* 0.505738* 0.461468* 0.611805* 0.532720* 0.512996* 0.546657* 0.558234* 0.528619* 0.509953* 0.527413* 0.587818*
Lipid Particles 0.021440 0.062687 0.130709* 0.037821 0.046699 0.036039 0.107958* 0.038495 0.046301 0.047213 0.113098* 0.038122
Mitochondria 0.004264 0.000627 0.000075 0.000935 0.000807 0.001256 0.000083 0.000651 0.000861 0.000992 0.000081 0.000808
Mitotic Spindle 0.000597 0.000965 0.000905 0.001226 0.000288 0.000693 0.002898 0.001880 0.000292 0.000807 0.002448 0.001519
None 0.001362 0.000017 0.000010 0.000030 0.000010 0.000015 0.000016 0.000021 0.000031 0.000016 0.000015 0.000026
Nuclear Periphery 0.001480 0.000020 0.000130 0.000057 0.000016 0.000019 0.000084 0.000042 0.000039 0.000019 0.000095 0.000050
Nuclear Periphery Foci 0.052909 0.000144 0.001127 0.000336 0.000187 0.000245 0.000649 0.000289 0.001017 0.000202 0.000757 0.000315
Nucleolus 0.024261 0.000002 0.000001 0.000003 0.000001 0.000013 0.000001 0.000003 0.000382 0.000008 0.000001 0.000003
Nucleus 0.083006 0.000017 0.000015 0.000020 0.000010 0.000028 0.000017 0.000019 0.001316 0.000024 0.000017 0.000019
Peroxisomes 0.002753 0.002442 0.000585 0.001230 0.001172 0.002300 0.000624 0.003614 0.001197 0.002360 0.000615 0.002297
Vacuole 0.098750 0.001216 0.008957 0.005778 0.001127 0.001058 0.005171 0.005455 0.002663 0.001124 0.006026 0.005634
Vacuole Periphery 0.013016 0.001120 0.001886 0.002644 0.001340 0.000983 0.001444 0.002649 0.001524 0.001040 0.001544 0.002647

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.42 -1.12 -5.26 -3.23 -5.05 -5.64 -4.71 -6.99 -2.04 -1.09 -5.30 -4.01 -8.18 -3.31 -2.14
Bud Neck 3.25 2.79 3.10 -5.57 3.43 0.56 -4.75 -3.89 -4.28 0.71 2.19 -4.90 -3.30 -6.43 2.56
Bud Site 2.79 2.11 1.80 -6.47 -1.16 -2.51 -2.88 -8.08 -6.83 -6.38 -1.32 -2.91 -9.42 -8.94 -6.13
Cell Periphery 2.84 2.44 1.19 -7.14 -4.36 1.39 -2.12 -3.02 -3.28 -2.58 1.54 -1.97 -6.12 -6.79 -5.36
Cytoplasm 2.10 2.11 2.08 -3.30 -5.22 -5.01 2.62 -6.70 -5.67 -7.01 1.54 2.15 -0.43 -6.38 -8.53
Cytoplasmic Foci -3.96 -1.74 -0.53 20.56 3.30 1.17 22.74 16.22 16.64 -7.89 2.74 20.66 22.62 27.52 -2.75
Eisosomes 1.02 1.68 -0.43 -4.13 -5.31 -4.27 -2.19 -2.67 1.14 -0.30 -4.13 -1.64 -6.35 -3.18 -4.47
Endoplasmic Reticulum 2.17 1.96 2.05 -3.32 1.14 0.29 -3.70 2.50 1.62 5.08 1.15 -3.21 -0.82 -2.25 2.65
Endosome -2.39 -3.52 -1.22 6.74 6.01 -1.01 0.93 -0.19 0.82 -1.10 -2.95 -1.76 1.23 5.38 3.00
Golgi -4.67 -3.75 -6.51 -12.73 -8.12 2.63 -1.23 -2.85 -5.41 -0.96 2.63 0.09 -8.23 -13.37 -6.36
Lipid Particles -4.35 -6.78 -1.97 5.53 6.72 2.60 -8.84 1.93 -0.72 10.48 -0.24 -10.27 2.21 3.29 12.75
Mitochondria 0.94 1.11 0.85 -0.75 -2.82 -0.67 1.66 0.32 1.09 -2.91 -0.27 1.78 0.09 0.50 -3.83
Mitotic Spindle -0.60 -0.63 -1.47 -0.48 -0.72 -0.85 -1.73 -3.60 -2.08 0.60 -1.34 -1.83 -3.96 -1.79 0.73
None 1.96 1.97 1.94 -3.49 -10.07 -1.84 -4.89 -6.48 -1.60 -2.74 1.33 1.46 0.56 -3.92 -8.15
Nuclear Periphery 1.91 1.77 1.86 -2.72 3.58 -0.38 -5.87 -2.98 -2.14 2.91 1.39 -3.51 -0.82 -3.39 3.54
Nuclear Periphery Foci 2.71 2.67 2.70 -3.27 2.18 -0.43 -2.32 -1.07 -0.38 1.79 2.26 0.94 2.00 -1.42 2.59
Nucleolus 2.39 2.39 2.39 -0.81 -2.24 -1.14 -1.73 -2.95 0.97 -1.59 2.12 2.16 2.15 0.92 -2.32
Nucleus 3.01 3.01 3.01 -0.37 -2.55 -1.67 -6.48 -3.96 0.92 -1.12 2.54 2.55 2.55 0.80 -2.08
Peroxisomes 0.83 2.33 1.82 3.00 -4.87 -4.76 4.33 -4.49 -2.28 -5.55 -4.98 4.93 -4.10 0.19 -6.68
Vacuole 3.14 2.95 3.02 -7.53 2.15 -0.18 -6.54 -8.85 -7.92 -0.48 2.45 -2.95 -2.89 -10.60 0.45
Vacuole Periphery 2.03 1.88 1.74 -4.71 -2.06 1.70 -0.52 -2.55 -3.37 -2.47 2.11 -0.18 -3.46 -5.73 -3.97