Standard name | |
---|---|
Human Ortholog | |
Description | Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress; mutation is functionally complemented by human FKBP2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 2 | 5 | 2 | 3 | 8 | 5 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 2 | 3 | 1 | 1 | 0 | 2 |
Bud | 2 | 1 | 1 | 3 | 3 | 18 | 25 | 21 | 12 | 15 | 16 | 13 | 1 | 3 | 0 | 2 | 1 | 0 | 3 | 3 | 4 | 1 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 4 | 7 | 1 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 11 | 7 | 1 | 12 | 6 | 11 | 10 | 23 | 50 | 62 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 371 | 436 | 294 | 274 | 286 | 395 | 549 | 591 | 399 | 480 | 305 | 377 | 310 | 437 | 531 | 153 | 109 | 100 | 67 | 127 | 43 | 52 | 26 | 27 |
Endoplasmic Reticulum | 26 | 8 | 7 | 3 | 3 | 10 | 4 | 5 | 2 | 3 | 0 | 4 | 66 | 41 | 85 | 9 | 10 | 11 | 87 | 39 | 47 | 79 | 38 | 62 |
Endosome | 4 | 2 | 12 | 5 | 2 | 4 | 3 | 6 | 3 | 7 | 0 | 2 | 1 | 1 | 1 | 14 | 7 | 3 | 11 | 11 | 12 | 5 | 8 | 16 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 |
Mitochondria | 1 | 5 | 1 | 1 | 3 | 10 | 18 | 23 | 32 | 39 | 21 | 28 | 0 | 0 | 0 | 13 | 1 | 5 | 2 | 8 | 5 | 9 | 6 | 11 |
Nucleus | 10 | 1 | 1 | 0 | 3 | 5 | 14 | 13 | 8 | 8 | 2 | 8 | 3 | 2 | 4 | 129 | 94 | 72 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 2 | 0 |
Vac/Vac Membrane | 103 | 36 | 89 | 45 | 26 | 27 | 56 | 52 | 53 | 86 | 43 | 58 | 1 | 7 | 5 | 258 | 202 | 154 | 338 | 304 | 288 | 175 | 197 | 277 |
Unique Cell Count | 474 | 475 | 373 | 301 | 306 | 433 | 623 | 659 | 461 | 559 | 347 | 428 | 371 | 495 | 611 | 446 | 340 | 276 | 531 | 519 | 411 | 334 | 294 | 416 |
Labelled Cell Count | 519 | 490 | 406 | 332 | 327 | 484 | 689 | 720 | 525 | 659 | 403 | 501 | 406 | 547 | 690 | 580 | 425 | 345 | 531 | 519 | 411 | 334 | 294 | 416 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 5.4 | 5.2 | 5.9 | 5.9 | 6.0 | 5.2 | 5.5 | 5.3 | 5.2 | 5.3 | 5.5 | 7.5 | 7.3 | 7.3 | 7.6 | 7.6 | 7.6 | 8.2 | 8.4 | 8.4 |
Std Deviation (1e-4) | 1.1 | 0.9 | 1.1 | 1.8 | 1.4 | 1.5 | 1.3 | 1.3 | 1.3 | 1.3 | 1.2 | 1.5 | 1.2 | 1.1 | 1.2 | 1.5 | 1.8 | 1.8 | 1.5 | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.17 | 0.2 | -0.0 | 0.07 | 0.01 | -0.02 | 0.02 | 0.08 | 0.52 | 0.48 | 0.48 | 0.53 | 0.54 | 0.54 | 0.65 | 0.68 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 3.6 | 3.6 | 3.1 | 2.7 | 2.8 | 3.8 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 3.1 | 0 | 0 | 3.1 | 0 | 3.5 | 0 | 4.9 | 6.3 | 6.4 | 0 | 0 | 0 |
Cytoplasm | 4.3 | 5.4 | 5.0 | 3.9 | 4.8 | 3.0 | 2.8 | 3.3 | 3.5 | 1.7 | 3.8 | 3.3 | -12.7 | -12.6 | -11.0 |
Endoplasmic Reticulum | 0 | 0 | 0.4 | 0 | 0 | 0 | 0 | 0 | -1.1 | 7.3 | 4.1 | 6.3 | 0.1 | 0.9 | 1.6 |
Endosome | -1.3 | -2.3 | -2.3 | -3.4 | -2.7 | -2.8 | -2.1 | -3.4 | -3.0 | -3.1 | -3.6 | 0 | -0.1 | -1.0 | -1.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.5 | 2.9 | 3.3 | 4.9 | 5.0 | 4.5 | 4.7 | 0 | 0 | 0 | 2.9 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 2.5 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11.2 | 10.7 | 10.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.9 | -5.3 | -7.1 | -6.4 | -7.2 | -4.7 | -3.2 | -4.0 | -3.8 | -9.9 | -10.4 | -11.9 | 9.8 | 9.6 | 8.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2498 | 3.7813 | 3.9753 | 3.6078 | 3.2966 | 3.8586 | 6.4365 | 7.709 | 7.2339 | 6.7556 | 6.5723 | 7.3573 | 5.5123 | 6.0852 | 5.6866 | 4.764 | 5.2203 | 5.2878 |
Actin | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0041 | 0.0002 | 0.0237 | 0.0106 | 0.1586 | 0.0028 |
Bud | 0.0016 | 0.0022 | 0.0009 | 0.0009 | 0.001 | 0.0014 | 0.0002 | 0.002 | 0.0005 | 0.0003 | 0.0008 | 0.0004 | 0.0008 | 0.0002 | 0.001 | 0.0003 | 0.0028 | 0.0003 |
Bud Neck | 0.0013 | 0.0042 | 0.0045 | 0.0018 | 0.0031 | 0.0039 | 0.0004 | 0.0019 | 0.0015 | 0.0017 | 0.0019 | 0.0037 | 0.0017 | 0.0015 | 0.0041 | 0.001 | 0.0022 | 0.006 |
Bud Periphery | 0.0024 | 0.0019 | 0.0018 | 0.0011 | 0.0026 | 0.0035 | 0.0007 | 0.0017 | 0.0014 | 0.0006 | 0.0014 | 0.0014 | 0.0014 | 0.0006 | 0.0016 | 0.0005 | 0.0034 | 0.0009 |
Bud Site | 0.01 | 0.0103 | 0.0043 | 0.0025 | 0.0023 | 0.0015 | 0.0006 | 0.0057 | 0.0018 | 0.0013 | 0.0033 | 0.001 | 0.002 | 0.0026 | 0.0085 | 0.001 | 0.0028 | 0.0008 |
Cell Periphery | 0.0034 | 0.0034 | 0.0034 | 0.0013 | 0.0026 | 0.0043 | 0.0063 | 0.0072 | 0.0061 | 0.0068 | 0.0048 | 0.0052 | 0.0055 | 0.0095 | 0.0073 | 0.0044 | 0.0013 | 0.0036 |
Cytoplasm | 0.1909 | 0.1995 | 0.2179 | 0.1743 | 0.1289 | 0.1947 | 0.2207 | 0.2382 | 0.2215 | 0.1737 | 0.1816 | 0.2563 | 0.2045 | 0.2629 | 0.1891 | 0.1252 | 0.1093 | 0.1623 |
Cytoplasmic Foci | 0.0241 | 0.0182 | 0.0233 | 0.024 | 0.0139 | 0.0212 | 0.0118 | 0.0167 | 0.0157 | 0.0065 | 0.0136 | 0.0078 | 0.021 | 0.0126 | 0.0277 | 0.0164 | 0.022 | 0.0153 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.1372 | 0.073 | 0.1388 | 0.0566 | 0.0623 | 0.1206 | 0.3785 | 0.2727 | 0.2733 | 0.4155 | 0.2584 | 0.2997 | 0.1889 | 0.2339 | 0.2609 | 0.214 | 0.06 | 0.2728 |
Endosome | 0.1765 | 0.1413 | 0.1839 | 0.2254 | 0.1671 | 0.1444 | 0.0912 | 0.079 | 0.1193 | 0.0713 | 0.1277 | 0.0533 | 0.1074 | 0.0579 | 0.0883 | 0.1615 | 0.1288 | 0.0822 |
Golgi | 0.0024 | 0.0023 | 0.0039 | 0.0027 | 0.0021 | 0.0023 | 0.0022 | 0.0021 | 0.0038 | 0.001 | 0.0026 | 0.0013 | 0.0106 | 0.0012 | 0.008 | 0.0039 | 0.016 | 0.007 |
Lipid Particles | 0.0088 | 0.0041 | 0.0051 | 0.0016 | 0.0027 | 0.0053 | 0.0157 | 0.0147 | 0.0214 | 0.0111 | 0.0057 | 0.0054 | 0.0197 | 0.0127 | 0.0282 | 0.0152 | 0.0108 | 0.0111 |
Mitochondria | 0.0029 | 0.0018 | 0.0061 | 0.001 | 0.001 | 0.0032 | 0.0007 | 0.0005 | 0.0012 | 0.0002 | 0.0008 | 0.0008 | 0.0036 | 0.0003 | 0.0057 | 0.0011 | 0.0071 | 0.0021 |
None | 0.0217 | 0.0259 | 0.0193 | 0.0016 | 0.0025 | 0.0218 | 0.0183 | 0.0081 | 0.0045 | 0.0009 | 0.016 | 0.0103 | 0.0298 | 0.0105 | 0.0178 | 0.0083 | 0.0026 | 0.0036 |
Nuclear Periphery | 0.019 | 0.0157 | 0.0189 | 0.011 | 0.0136 | 0.013 | 0.0136 | 0.0118 | 0.0113 | 0.0103 | 0.0192 | 0.0133 | 0.0373 | 0.017 | 0.0154 | 0.0286 | 0.0343 | 0.0242 |
Nucleolus | 0.0026 | 0.003 | 0.0026 | 0.0007 | 0.0014 | 0.0022 | 0.0003 | 0.0023 | 0.0009 | 0.0003 | 0.0007 | 0.0007 | 0.0012 | 0.0008 | 0.0015 | 0.0007 | 0.001 | 0.0005 |
Nucleus | 0.0141 | 0.0194 | 0.0128 | 0.0063 | 0.0093 | 0.0194 | 0.0034 | 0.0092 | 0.004 | 0.0036 | 0.0084 | 0.0067 | 0.052 | 0.0056 | 0.0065 | 0.0078 | 0.0209 | 0.0109 |
Peroxisomes | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0012 | 0.0001 | 0.0048 | 0.001 | 0.0459 | 0.0054 |
Punctate Nuclear | 0.0012 | 0.0013 | 0.0016 | 0.0003 | 0.0005 | 0.0015 | 0.0004 | 0.0017 | 0.0003 | 0.0003 | 0.0007 | 0.0005 | 0.0045 | 0.0005 | 0.002 | 0.0079 | 0.0065 | 0.001 |
Vacuole | 0.3587 | 0.4547 | 0.3292 | 0.4558 | 0.5562 | 0.4145 | 0.2261 | 0.3177 | 0.2993 | 0.2882 | 0.3414 | 0.3223 | 0.2876 | 0.361 | 0.2852 | 0.3756 | 0.3389 | 0.3738 |
Vacuole Periphery | 0.0208 | 0.0173 | 0.0211 | 0.0307 | 0.0267 | 0.0206 | 0.0077 | 0.0064 | 0.012 | 0.0064 | 0.0107 | 0.0095 | 0.0149 | 0.0084 | 0.0127 | 0.0151 | 0.0247 | 0.0134 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 94.6548 | 60.4397 | 77.1401 | 81.1515 | 87.6601 | 97.3053 | 91.6658 | 80.8412 | 94.7312 | 91.8758 |
Translational Efficiency | 1.661 | 2.1227 | 1.578 | 1.7857 | 1.5763 | 1.5471 | 1.3445 | 1.5398 | 1.4631 | 1.3318 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
669 | 1987 | 1365 | 169 | 1607 | 1869 | 324 | 1385 | 2276 | 3856 | 1689 | 1554 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 748.56 | 847.03 | 1025.32 | 967.34 | 775.33 | 822.99 | 1086.40 | 1068.04 | 767.46 | 835.38 | 1037.04 | 1057.09 |
Standard Deviation | 88.92 | 130.24 | 153.06 | 119.43 | 111.81 | 117.96 | 127.98 | 185.77 | 106.30 | 125.02 | 150.51 | 182.46 |
Intensity Change Log 2 | — | 0.178295 | 0.453884 | 0.369905 | — | 0.086064 | 0.486673 | 0.462083 | — | 0.132107 | 0.470660 | 0.417539 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000070 | 0.000423 | 0.001996 | 0.000585 | 0.000098 | 0.000231 | 0.001279 | 0.005771 | 0.000090 | 0.000330 | 0.001858 | 0.005207 |
Bud Neck | 0.008565 | 0.013622 | 0.006214 | 0.004246 | 0.010217 | 0.013994 | 0.003786 | 0.016180 | 0.009731 | 0.013802 | 0.005748 | 0.014883 |
Bud Site | 0.002736 | 0.012198 | 0.017894 | 0.006308 | 0.006193 | 0.022095 | 0.010646 | 0.049804 | 0.005177 | 0.016995 | 0.016503 | 0.045074 |
Cell Periphery | 0.000347 | 0.000769 | 0.002480 | 0.001012 | 0.000320 | 0.000942 | 0.002527 | 0.011297 | 0.000328 | 0.000853 | 0.002489 | 0.010179 |
Cytoplasm | 0.550495* | 0.322678* | 0.238806* | 0.181118* | 0.292490* | 0.229713* | 0.160606* | 0.335854* | 0.368327* | 0.277618* | 0.223805* | 0.319026* |
Cytoplasmic Foci | 0.198920* | 0.094804 | 0.020988 | 0.081330 | 0.106804 | 0.073489 | 0.017991 | 0.042866 | 0.133880 | 0.084473 | 0.020413 | 0.047049 |
Eisosomes | 0.000258 | 0.000250 | 0.000372 | 0.000341 | 0.000130 | 0.000331 | 0.000308 | 0.001298 | 0.000168 | 0.000289 | 0.000359 | 0.001194 |
Endoplasmic Reticulum | 0.007641 | 0.069087 | 0.539648* | 0.069690 | 0.042640 | 0.122468* | 0.629295* | 0.258232* | 0.032353 | 0.094961 | 0.556845* | 0.237727* |
Endosome | 0.014079 | 0.031558 | 0.007834 | 0.023549 | 0.046603 | 0.046659 | 0.006954 | 0.013498 | 0.037043 | 0.038877 | 0.007665 | 0.014591 |
Golgi | 0.000952 | 0.003651 | 0.001448 | 0.000347 | 0.001705 | 0.004591 | 0.000980 | 0.004801 | 0.001484 | 0.004107 | 0.001358 | 0.004316 |
Lipid Particles | 0.015693 | 0.014691 | 0.013010 | 0.021031 | 0.011798 | 0.017555 | 0.010736 | 0.039367 | 0.012943 | 0.016079 | 0.012574 | 0.037373 |
Mitochondria | 0.000299 | 0.002319 | 0.000415 | 0.000178 | 0.001094 | 0.003627 | 0.000253 | 0.001894 | 0.000860 | 0.002953 | 0.000384 | 0.001707 |
Mitotic Spindle | 0.000030 | 0.001363 | 0.000594 | 0.002355 | 0.000095 | 0.004509 | 0.000098 | 0.002122 | 0.000076 | 0.002887 | 0.000499 | 0.002147 |
None | 0.003097 | 0.001819 | 0.000839 | 0.000163 | 0.002075 | 0.001784 | 0.000366 | 0.000989 | 0.002375 | 0.001802 | 0.000749 | 0.000899 |
Nuclear Periphery | 0.000720 | 0.000655 | 0.003724 | 0.005855 | 0.000949 | 0.001653 | 0.002758 | 0.000371 | 0.000881 | 0.001139 | 0.003539 | 0.000967 |
Nuclear Periphery Foci | 0.003413 | 0.001962 | 0.027591 | 0.040023 | 0.002042 | 0.001712 | 0.026673 | 0.009818 | 0.002445 | 0.001841 | 0.027415 | 0.013103 |
Nucleolus | 0.000591 | 0.000791 | 0.000104 | 0.000519 | 0.000914 | 0.000902 | 0.000077 | 0.000248 | 0.000819 | 0.000845 | 0.000098 | 0.000278 |
Nucleus | 0.098480 | 0.037664 | 0.045581 | 0.087867 | 0.027264 | 0.023670 | 0.033806 | 0.028115 | 0.048197 | 0.030881 | 0.043322 | 0.034613 |
Peroxisomes | 0.001028 | 0.001688 | 0.000479 | 0.001474 | 0.001372 | 0.001666 | 0.000362 | 0.002451 | 0.001270 | 0.001677 | 0.000457 | 0.002344 |
Vacuole | 0.092177 | 0.384412* | 0.069572 | 0.471712* | 0.442317* | 0.417157* | 0.090284 | 0.173789 | 0.339398* | 0.400284* | 0.073545 | 0.206188* |
Vacuole Periphery | 0.000408 | 0.003595 | 0.000412 | 0.000299 | 0.002881 | 0.011252 | 0.000215 | 0.001235 | 0.002154 | 0.007306 | 0.000374 | 0.001133 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.92 | -4.80 | -3.94 | -0.90 | 3.38 | -5.34 | -2.77 | -9.68 | -9.45 | -6.00 | -3.77 | -5.29 | -9.77 | -9.23 | -5.18 |
Bud Neck | -2.61 | 1.06 | 2.15 | 6.68 | 1.65 | -3.05 | 5.23 | -4.18 | -1.19 | -7.89 | -4.14 | 3.66 | -3.88 | -0.64 | -6.75 |
Bud Site | -9.25 | -7.61 | -2.01 | 2.90 | 4.48 | -8.00 | -1.44 | -13.41 | -7.44 | -8.52 | -10.48 | -6.55 | -13.71 | -9.16 | -8.32 |
Cell Periphery | -6.55 | -8.78 | -6.73 | -1.81 | 5.88 | -8.75 | -3.15 | -11.77 | -11.01 | -7.19 | -10.59 | -9.09 | -11.79 | -11.09 | -8.59 |
Cytoplasm | 22.67 | 28.36 | 24.12 | 10.30 | 4.20 | 7.80 | 9.82 | -4.69 | -11.88 | -12.59 | 13.44 | 17.39 | 5.62 | -5.44 | -10.38 |
Cytoplasmic Foci | 16.48 | 30.27 | 14.66 | 2.39 | -9.66 | 8.53 | 23.31 | 16.82 | 9.05 | -8.15 | 14.47 | 36.19 | 24.46 | 13.30 | -11.20 |
Eisosomes | 0.45 | -2.12 | -2.46 | -2.47 | 0.65 | -4.27 | -1.96 | -10.87 | -8.07 | -6.77 | -4.57 | -4.23 | -10.66 | -9.00 | -7.67 |
Endoplasmic Reticulum | -15.18 | -49.63* | -4.82 | 0.49 | 30.33* | -12.94 | -26.99 | -20.71 | -11.28 | 16.54 | -15.54 | -53.11 | -22.12 | -14.32 | 26.26 |
Endosome | -11.42 | 5.10 | -4.96 | 4.69 | -9.59 | -0.09 | 24.00 | 19.27 | 19.59 | -6.46 | -1.22 | 22.89 | 16.55 | 20.86 | -7.48 |
Golgi | -9.00 | -2.80 | 5.77 | 11.68 | 7.67 | -6.57 | 2.96 | -5.24 | 0.25 | -7.00 | -9.29 | 0.76 | -5.53 | 0.20 | -6.00 |
Lipid Particles | 0.54 | 1.06 | -1.54 | -2.75 | -3.05 | -5.48 | 0.69 | -10.55 | -8.03 | -10.28 | -3.44 | 0.07 | -10.16 | -9.00 | -9.78 |
Mitochondria | -7.62 | -2.76 | 0.92 | 7.40 | 1.95 | -5.35 | 3.31 | -1.14 | 2.72 | -2.76 | -7.06 | 2.64 | -1.43 | 2.47 | -2.47 |
Mitotic Spindle | -2.11 | -3.45 | -1.06 | -0.37 | -0.68 | -3.75 | -0.18 | -3.21 | 1.56 | -3.20 | -4.28 | -3.24 | -3.40 | 0.57 | -2.48 |
None | 1.54 | 2.95 | 3.90 | 5.18 | 4.24 | 0.32 | 2.50 | 1.67 | 1.32 | -2.01 | 0.91 | 3.07 | 2.81 | 2.50 | -0.43 |
Nuclear Periphery | 0.61 | -7.85 | -4.34 | -4.41 | -1.44 | -3.75 | -3.49 | 5.62 | 7.78 | 4.62 | -2.32 | -8.18 | -0.29 | 1.44 | 7.69 |
Nuclear Periphery Foci | 3.74 | -15.63 | -9.17 | -9.60 | -2.40 | 1.52 | -9.27 | -11.91 | -12.33 | 6.15 | 3.27 | -18.80 | -13.99 | -14.91 | 9.70 |
Nucleolus | -3.61 | 12.56 | 1.00 | 3.24 | -4.94 | 0.09 | 5.64 | 4.51 | 3.67 | -6.87 | -0.17 | 6.83 | 5.13 | 6.30 | -8.20 |
Nucleus | 15.80 | 11.85 | 1.18 | -5.75 | -4.47 | 2.30 | -1.96 | -1.41 | -3.52 | 1.42 | 9.84 | 0.88 | 5.38 | -2.79 | 3.65 |
Peroxisomes | -5.01 | 4.84 | -0.72 | 0.53 | -1.71 | -1.94 | 8.07 | -4.47 | -3.10 | -9.90 | -3.72 | 7.98 | -4.99 | -2.91 | -9.39 |
Vacuole | -38.29* | 2.93 | -20.54 | -5.20 | -21.73 | 2.47 | 30.81* | 27.30 | 23.91 | -8.89 | -8.01 | 37.89 | 13.20 | 22.65 | -20.96 |
Vacuole Periphery | -14.39 | -0.03 | 2.81 | 14.97 | 1.50 | -10.24 | 15.51 | 7.82 | 12.41 | -6.88 | -12.06 | 12.96 | 6.21 | 14.57 | -5.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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