Standard name
Description Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress; mutation is functionally complemented by human FKBP2

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.1 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.92 0.79 0.91 0.93 0.91 0.88 0.9 0.87 0.86 0.88 0.88 0.84 0.88 0.87 0.34 0.32 0.36 0.13 0.25 0.11 0.16 0.09 0.07
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0.18 0.08 0.14 0 0 0 0.17 0.08 0.12 0.24 0.13 0.15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0.07 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.29 0.28 0.26 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.22 0.08 0.24 0.15 0.08 0.06 0.09 0.08 0.11 0.15 0.12 0.14 0 0 0 0.58 0.59 0.56 0.64 0.59 0.7 0.52 0.67 0.67
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 2 5 2 3 8 5 1 1 2 2 1 0 0 2 3 1 1 0 2
Bud 2 1 1 3 3 18 25 21 12 15 16 13 1 3 0 2 1 0 3 3 4 1 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0 1 0 4 7 1 1 2 1
Bud Site 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 0 0 0
Cell Periphery 1 0 0 0 0 11 7 1 12 6 11 10 23 50 62 0 0 0 0 0 0 0 0 0
Cytoplasm 371 436 294 274 286 395 549 591 399 480 305 377 310 437 531 153 109 100 67 127 43 52 26 27
Endoplasmic Reticulum 26 8 7 3 3 10 4 5 2 3 0 4 66 41 85 9 10 11 87 39 47 79 38 62
Endosome 4 2 12 5 2 4 3 6 3 7 0 2 1 1 1 14 7 3 11 11 12 5 8 16
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 1 0 0 0 2
Mitochondria 1 5 1 1 3 10 18 23 32 39 21 28 0 0 0 13 1 5 2 8 5 9 6 11
Nucleus 10 1 1 0 3 5 14 13 8 8 2 8 3 2 4 129 94 72 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 1 0 6 0 0 2 0 0 0 1 0 0 0 0 3 0 1 0 1 1
Nucleolus 0 0 0 1 0 2 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 2 4 0 0 2 0
Vac/Vac Membrane 103 36 89 45 26 27 56 52 53 86 43 58 1 7 5 258 202 154 338 304 288 175 197 277
Unique Cell Count 474 475 373 301 306 433 623 659 461 559 347 428 371 495 611 446 340 276 531 519 411 334 294 416
Labelled Cell Count 519 490 406 332 327 484 689 720 525 659 403 501 406 547 690 580 425 345 531 519 411 334 294 416


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 5.4 5.2 5.9 5.9 6.0 5.2 5.5 5.3 5.2 5.3 5.5 7.5 7.3 7.3 7.6 7.6 7.6 8.2 8.4 8.4
Std Deviation (1e-4) 1.1 0.9 1.1 1.8 1.4 1.5 1.3 1.3 1.3 1.3 1.2 1.5 1.2 1.1 1.2 1.5 1.8 1.8 1.5 1.5 1.9
Intensity Change (Log2) 0.18 0.17 0.2 -0.0 0.07 0.01 -0.02 0.02 0.08 0.52 0.48 0.48 0.53 0.54 0.54 0.65 0.68 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 3.6 3.6 3.1 2.7 2.8 3.8 3.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3.1 0 0 3.1 0 3.5 0 4.9 6.3 6.4 0 0 0
Cytoplasm 4.3 5.4 5.0 3.9 4.8 3.0 2.8 3.3 3.5 1.7 3.8 3.3 -12.7 -12.6 -11.0
Endoplasmic Reticulum 0 0 0.4 0 0 0 0 0 -1.1 7.3 4.1 6.3 0.1 0.9 1.6
Endosome -1.3 -2.3 -2.3 -3.4 -2.7 -2.8 -2.1 -3.4 -3.0 -3.1 -3.6 0 -0.1 -1.0 -1.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.5 2.9 3.3 4.9 5.0 4.5 4.7 0 0 0 2.9 0 0
Nucleus 0 0 0 2.5 2.3 0 0 0 0 0 0 0 11.2 10.7 10.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.9 -5.3 -7.1 -6.4 -7.2 -4.7 -3.2 -4.0 -3.8 -9.9 -10.4 -11.9 9.8 9.6 8.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fpr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available