Standard name
Human Ortholog
Description Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress; mutation is functionally complemented by human FKBP2

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.1 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.92 0.79 0.91 0.93 0.91 0.88 0.9 0.87 0.86 0.88 0.88 0.84 0.88 0.87 0.34 0.32 0.36 0.13 0.25 0.11 0.16 0.09 0.07
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0.18 0.08 0.14 0 0 0 0.17 0.08 0.12 0.24 0.13 0.15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0.07 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.29 0.28 0.26 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.22 0.08 0.24 0.15 0.08 0.06 0.09 0.08 0.11 0.15 0.12 0.14 0 0 0 0.58 0.59 0.56 0.64 0.59 0.7 0.52 0.67 0.67
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 2 5 2 3 8 5 1 1 2 2 1 0 0 2 3 1 1 0 2
Bud 2 1 1 3 3 18 25 21 12 15 16 13 1 3 0 2 1 0 3 3 4 1 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0 1 0 4 7 1 1 2 1
Bud Site 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 0 0 0
Cell Periphery 1 0 0 0 0 11 7 1 12 6 11 10 23 50 62 0 0 0 0 0 0 0 0 0
Cytoplasm 371 436 294 274 286 395 549 591 399 480 305 377 310 437 531 153 109 100 67 127 43 52 26 27
Endoplasmic Reticulum 26 8 7 3 3 10 4 5 2 3 0 4 66 41 85 9 10 11 87 39 47 79 38 62
Endosome 4 2 12 5 2 4 3 6 3 7 0 2 1 1 1 14 7 3 11 11 12 5 8 16
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 1 0 0 0 2
Mitochondria 1 5 1 1 3 10 18 23 32 39 21 28 0 0 0 13 1 5 2 8 5 9 6 11
Nucleus 10 1 1 0 3 5 14 13 8 8 2 8 3 2 4 129 94 72 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 1 0 6 0 0 2 0 0 0 1 0 0 0 0 3 0 1 0 1 1
Nucleolus 0 0 0 1 0 2 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 2 4 0 0 2 0
Vac/Vac Membrane 103 36 89 45 26 27 56 52 53 86 43 58 1 7 5 258 202 154 338 304 288 175 197 277
Unique Cell Count 474 475 373 301 306 433 623 659 461 559 347 428 371 495 611 446 340 276 531 519 411 334 294 416
Labelled Cell Count 519 490 406 332 327 484 689 720 525 659 403 501 406 547 690 580 425 345 531 519 411 334 294 416


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 5.4 5.2 5.9 5.9 6.0 5.2 5.5 5.3 5.2 5.3 5.5 7.5 7.3 7.3 7.6 7.6 7.6 8.2 8.4 8.4
Std Deviation (1e-4) 1.1 0.9 1.1 1.8 1.4 1.5 1.3 1.3 1.3 1.3 1.2 1.5 1.2 1.1 1.2 1.5 1.8 1.8 1.5 1.5 1.9
Intensity Change (Log2) 0.18 0.17 0.2 -0.0 0.07 0.01 -0.02 0.02 0.08 0.52 0.48 0.48 0.53 0.54 0.54 0.65 0.68 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 3.6 3.6 3.1 2.7 2.8 3.8 3.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3.1 0 0 3.1 0 3.5 0 4.9 6.3 6.4 0 0 0
Cytoplasm 4.3 5.4 5.0 3.9 4.8 3.0 2.8 3.3 3.5 1.7 3.8 3.3 -12.7 -12.6 -11.0
Endoplasmic Reticulum 0 0 0.4 0 0 0 0 0 -1.1 7.3 4.1 6.3 0.1 0.9 1.6
Endosome -1.3 -2.3 -2.3 -3.4 -2.7 -2.8 -2.1 -3.4 -3.0 -3.1 -3.6 0 -0.1 -1.0 -1.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.5 2.9 3.3 4.9 5.0 4.5 4.7 0 0 0 2.9 0 0
Nucleus 0 0 0 2.5 2.3 0 0 0 0 0 0 0 11.2 10.7 10.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.9 -5.3 -7.1 -6.4 -7.2 -4.7 -3.2 -4.0 -3.8 -9.9 -10.4 -11.9 9.8 9.6 8.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2498 3.7813 3.9753 3.6078 3.2966 3.8586 6.4365 7.709 7.2339 6.7556 6.5723 7.3573 5.5123 6.0852 5.6866 4.764 5.2203 5.2878
Actin 0.0001 0.0002 0.0003 0.0001 0 0.0001 0.0011 0.0002 0.0002 0.0001 0.0001 0.0001 0.0041 0.0002 0.0237 0.0106 0.1586 0.0028
Bud 0.0016 0.0022 0.0009 0.0009 0.001 0.0014 0.0002 0.002 0.0005 0.0003 0.0008 0.0004 0.0008 0.0002 0.001 0.0003 0.0028 0.0003
Bud Neck 0.0013 0.0042 0.0045 0.0018 0.0031 0.0039 0.0004 0.0019 0.0015 0.0017 0.0019 0.0037 0.0017 0.0015 0.0041 0.001 0.0022 0.006
Bud Periphery 0.0024 0.0019 0.0018 0.0011 0.0026 0.0035 0.0007 0.0017 0.0014 0.0006 0.0014 0.0014 0.0014 0.0006 0.0016 0.0005 0.0034 0.0009
Bud Site 0.01 0.0103 0.0043 0.0025 0.0023 0.0015 0.0006 0.0057 0.0018 0.0013 0.0033 0.001 0.002 0.0026 0.0085 0.001 0.0028 0.0008
Cell Periphery 0.0034 0.0034 0.0034 0.0013 0.0026 0.0043 0.0063 0.0072 0.0061 0.0068 0.0048 0.0052 0.0055 0.0095 0.0073 0.0044 0.0013 0.0036
Cytoplasm 0.1909 0.1995 0.2179 0.1743 0.1289 0.1947 0.2207 0.2382 0.2215 0.1737 0.1816 0.2563 0.2045 0.2629 0.1891 0.1252 0.1093 0.1623
Cytoplasmic Foci 0.0241 0.0182 0.0233 0.024 0.0139 0.0212 0.0118 0.0167 0.0157 0.0065 0.0136 0.0078 0.021 0.0126 0.0277 0.0164 0.022 0.0153
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0.0001 0 0.0004 0
Endoplasmic Reticulum 0.1372 0.073 0.1388 0.0566 0.0623 0.1206 0.3785 0.2727 0.2733 0.4155 0.2584 0.2997 0.1889 0.2339 0.2609 0.214 0.06 0.2728
Endosome 0.1765 0.1413 0.1839 0.2254 0.1671 0.1444 0.0912 0.079 0.1193 0.0713 0.1277 0.0533 0.1074 0.0579 0.0883 0.1615 0.1288 0.0822
Golgi 0.0024 0.0023 0.0039 0.0027 0.0021 0.0023 0.0022 0.0021 0.0038 0.001 0.0026 0.0013 0.0106 0.0012 0.008 0.0039 0.016 0.007
Lipid Particles 0.0088 0.0041 0.0051 0.0016 0.0027 0.0053 0.0157 0.0147 0.0214 0.0111 0.0057 0.0054 0.0197 0.0127 0.0282 0.0152 0.0108 0.0111
Mitochondria 0.0029 0.0018 0.0061 0.001 0.001 0.0032 0.0007 0.0005 0.0012 0.0002 0.0008 0.0008 0.0036 0.0003 0.0057 0.0011 0.0071 0.0021
None 0.0217 0.0259 0.0193 0.0016 0.0025 0.0218 0.0183 0.0081 0.0045 0.0009 0.016 0.0103 0.0298 0.0105 0.0178 0.0083 0.0026 0.0036
Nuclear Periphery 0.019 0.0157 0.0189 0.011 0.0136 0.013 0.0136 0.0118 0.0113 0.0103 0.0192 0.0133 0.0373 0.017 0.0154 0.0286 0.0343 0.0242
Nucleolus 0.0026 0.003 0.0026 0.0007 0.0014 0.0022 0.0003 0.0023 0.0009 0.0003 0.0007 0.0007 0.0012 0.0008 0.0015 0.0007 0.001 0.0005
Nucleus 0.0141 0.0194 0.0128 0.0063 0.0093 0.0194 0.0034 0.0092 0.004 0.0036 0.0084 0.0067 0.052 0.0056 0.0065 0.0078 0.0209 0.0109
Peroxisomes 0.0003 0.0002 0.0004 0.0003 0.0002 0.0005 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0012 0.0001 0.0048 0.001 0.0459 0.0054
Punctate Nuclear 0.0012 0.0013 0.0016 0.0003 0.0005 0.0015 0.0004 0.0017 0.0003 0.0003 0.0007 0.0005 0.0045 0.0005 0.002 0.0079 0.0065 0.001
Vacuole 0.3587 0.4547 0.3292 0.4558 0.5562 0.4145 0.2261 0.3177 0.2993 0.2882 0.3414 0.3223 0.2876 0.361 0.2852 0.3756 0.3389 0.3738
Vacuole Periphery 0.0208 0.0173 0.0211 0.0307 0.0267 0.0206 0.0077 0.0064 0.012 0.0064 0.0107 0.0095 0.0149 0.0084 0.0127 0.0151 0.0247 0.0134

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 94.6548 60.4397 77.1401 81.1515 87.6601 97.3053 91.6658 80.8412 94.7312 91.8758
Translational Efficiency 1.661 2.1227 1.578 1.7857 1.5763 1.5471 1.3445 1.5398 1.4631 1.3318

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress; mutation is functionally complemented by human FKBP2
Localization
Cell Percentages vacuole (39%), cytoplasm (13%), mixed (26%)
Cell Cycle Regulation Yes
vacuole - S/G2 (9.1e-06)
Subcompartmental Group N/A

Fpr2

Fpr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fpr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available