Standard name
Description Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0.07 0.05 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0.06 0 0 0.07 0.07 0.08 0.05 0.07 0.09 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 – 0.94 0.97 0.95 0.93 0.75 0.88 0.8 0.72 0.77 0.67 0.99 1.0 0.99 1.0 0.99 0.98 0.94 0.91 0.84 0.88 0.93 0.77
Endoplasmic Reticulum 0 – 0 0 0 0 0.14 0.05 0.13 0.1 0.12 0.17 0 0 0 0 0.08 0.06 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 3 1 3 7 4 13 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 1 0 1 2 0 0 0 0 0 0 0 0 0 0 0 2 2 6
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 1 1 2 3 8 9 4 7 7 19 1 0 2 1 2 1 0 0 0 1 1 4
Cytoplasm 119 – 15 74 109 39 86 97 66 76 62 80 91 68 104 90 88 135 122 27 20 78 100 102
Endoplasmic Reticulum 0 – 0 1 4 1 16 6 11 10 10 20 1 0 1 4 7 8 1 0 0 2 0 5
Endosome 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 1 1 3 6 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 1 0 5 2 6 1 0 0 0 0 0 0 1 0 0 0 0 2
Nucleus 0 – 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 – 0 0 0 0 0 0 0 5 0 3 0 0 0 0 0 2 0 0 0 0 0 5
Unique Cell Count 120 16 76 115 42 114 110 83 105 81 120 92 68 105 90 89 138 130 30 24 89 109 132
Labelled Cell Count 120 16 76 117 43 118 114 93 115 90 140 93 68 107 95 97 146 130 30 24 89 109 132


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 146.1 – 117.7 121.4 93.0 61.7 58.2 57.9 45.5 47.5 44.2 43.6 139.5 145.7 151.6 238.0 305.2 301.0 127.5 122.7 122.1
Std Deviation (1e-4) 36.2 – 37.1 41.8 38.2 25.3 23.8 20.5 19.3 18.1 13.7 12.3 40.2 44.3 37.8 69.9 71.5 89.1 41.7 54.3 60.6
Intensity Change (Log2) – – – 0.04 -0.34 -0.93 -1.02 -1.02 -1.37 -1.31 -1.41 -1.43 0.25 0.31 0.37 1.02 1.37 1.35 0.12 0.06 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.2 -0.1 -1.6 -0.7 -1.4 -1.8 -1.6 -2.2 1.4 2.1 1.5 2.4 1.4 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0