Standard name
Human Ortholog
Description Methylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Cytoplasm 0.88 0.99 0.99 0.92 0.85 0.85 0.71 0.73 0.68 0.67 0.68 0.99 0.99 0.97 0.94 0.94 0.89 0.83 0.89 0.95 0.9 0.89 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0 0.07 0.06 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0.09 0.19 0.09 0.3 0.2 0.22 0.24 0.18 0 0 0 0.09 0.09 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 3 1 0 0 0 0 0 0 1 1 1 0 1 3
Bud 0 3 0 0 0 2 0 0 0 1 4 0 0 0 0 0 0 1 3 1 13 7 18
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 2 3 2 7 1 6 7 4 8 9 2 2 8 9 3 0 0 0 0 0 0 0
Cytoplasm 92 232 286 197 205 169 233 157 149 157 173 287 325 351 102 44 16 120 212 276 295 272 260
Endoplasmic Reticulum 0 0 0 3 1 0 3 8 6 1 13 0 0 1 8 0 0 1 0 0 2 3 4
Endosome 1 0 0 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 5 0 1 1 4
Golgi 0 0 2 0 0 0 0 0 2 3 1 0 0 0 0 0 0 5 0 2 1 1 1
Mitochondria 4 0 0 3 3 24 13 15 14 20 16 1 0 1 2 0 0 1 2 1 1 2 3
Nucleus 1 1 2 0 1 3 5 1 5 5 4 1 3 2 0 0 0 1 5 1 0 0 1
Nuclear Periphery 2 0 0 0 0 1 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 2 2 2 1
Vac/Vac Membrane 6 4 0 19 46 17 99 42 48 55 46 1 1 7 10 4 2 4 2 0 5 8 5
Unique Cell Count 104 235 289 214 241 199 327 214 219 233 254 289 327 360 108 47 18 145 239 290 328 306 316
Labelled Cell Count 107 242 293 224 264 217 360 231 233 254 267 292 331 371 131 51 18 145 239 290 328 306 316


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.8 7.9 7.9 9.0 7.4 10.1 12.7 12.3 14.3 15.3 10.6 12.7 13.8 8.0 8.3 7.5 8.1 8.2 9.3
Std Deviation (1e-4) 1.7 2.0 1.7 2.0 2.3 2.7 2.9 5.2 4.5 6.0 6.1 3.4 5.0 6.1 3.1 2.4 1.7 6.9 6.4 7.0
Intensity Change (Log2) 0.01 0.19 -0.09 0.36 0.69 0.65 0.86 0.96 0.43 0.69 0.81 0.03 0.08 -0.07 0.05 0.06 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 2.0 0 0 0 0 0 0
Cytoplasm -3.9 -6.1 -6.1 -9.4 -8.8 -9.8 -10.0 -9.9 0.4 0.6 -1.4 -2.7 -2.6 -3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 3.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.1 3.4 4.6 4.4 5.1 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 5.2 7.8 5.1 10.2 7.9 8.4 8.7 7.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.5318 10.3283 10.0557 9.1321 8.2614 9.2261 3.3512 4.6689 4.2419 3.8793 4.4546 4.3679 16.9429 22.0674 19.2242 17.2103 14.8533 18.0471
Actin 0.0109 0.0002 0.0098 0.0021 0.0209 0.0027 0.0105 0.0011 0.0207 0.006 0.003 0.0038 0.0013 0.002 0.0035 0.0042 0.0005 0.0029
Bud 0.0008 0.0003 0.0012 0.0002 0.0009 0.0009 0.0009 0.0102 0.0132 0.0041 0.0052 0.0041 0.001 0.0023 0.0033 0.0116 0.0013 0.0007
Bud Neck 0.0007 0.0002 0.0009 0.0006 0.0011 0.0028 0.0069 0.0002 0.0006 0.0005 0.0012 0.003 0.0003 0.0002 0.0004 0.0021 0.0006 0.0012
Bud Periphery 0.0005 0.0001 0.0007 0.0001 0.0005 0.0005 0.0005 0.0022 0.0069 0.0015 0.002 0.002 0.0004 0.0006 0.0015 0.0103 0.0004 0.0004
Bud Site 0.0035 0.0003 0.0017 0.0002 0.0009 0.0004 0.0126 0.0078 0.0082 0.0013 0.0012 0.0004 0.0024 0.0031 0.0019 0.0019 0.0003 0.0002
Cell Periphery 0.0002 0 0.0001 0 0.0002 0.0001 0.0004 0.0004 0.0026 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0 0.0001
Cytoplasm 0.8897 0.987 0.9242 0.8935 0.8949 0.9301 0.7607 0.9315 0.8312 0.8262 0.775 0.8783 0.9544 0.9771 0.9558 0.9292 0.9726 0.9575
Cytoplasmic Foci 0.0143 0.001 0.0103 0.0071 0.0065 0.0117 0.0409 0.0036 0.0091 0.0032 0.0316 0.0291 0.0048 0.0017 0.0023 0.002 0.0022 0.0047
Eisosomes 0.0001 0 0.0002 0 0.0004 0 0 0 0.0002 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0056 0.0006 0.0045 0.0053 0.0036 0.0029 0.0057 0.001 0.002 0.0011 0.0303 0.0017 0.0021 0.0006 0.0012 0.0019 0.0015 0.0022
Endosome 0.0206 0.0003 0.006 0.041 0.0136 0.0093 0.0522 0.0008 0.0176 0.0232 0.0304 0.0136 0.0011 0.0003 0.0015 0.0007 0.0008 0.0016
Golgi 0.0036 0 0.0017 0.0056 0.0103 0.0019 0.0046 0.0001 0.0028 0.0003 0.001 0.0083 0.0001 0 0.0002 0.0001 0 0.0001
Lipid Particles 0.0041 0 0.0025 0.0061 0.0081 0.0017 0.0019 0.0001 0.0004 0 0.0006 0.0011 0.0001 0 0 0 0 0.0001
Mitochondria 0.0104 0.0001 0.0031 0.0031 0.009 0.0028 0.0012 0.0006 0.0028 0.0003 0.0018 0.0009 0.0002 0.0001 0.0011 0.0006 0.0001 0.0002
None 0.007 0.0014 0.0056 0.0016 0.0025 0.0048 0.0566 0.0242 0.0655 0.085 0.0401 0.0393 0.0102 0.0022 0.01 0.0046 0.0015 0.0083
Nuclear Periphery 0.0062 0.0011 0.0071 0.01 0.0099 0.0041 0.02 0.0008 0.0016 0.0012 0.0208 0.0011 0.003 0.0012 0.0019 0.0028 0.0025 0.0025
Nucleolus 0.0002 0 0.0002 0.0001 0.0004 0.0002 0.0005 0.0021 0.0008 0 0.0015 0.0003 0.0001 0 0.0001 0.0001 0 0
Nucleus 0.0126 0.0063 0.0128 0.0161 0.0102 0.0176 0.0083 0.0048 0.0064 0.0021 0.0159 0.0081 0.0148 0.0071 0.0133 0.0246 0.0135 0.0144
Peroxisomes 0.0018 0 0.0034 0.0007 0.0019 0.0007 0.0015 0.0002 0.0003 0.0001 0.0005 0.0004 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001
Punctate Nuclear 0.0025 0.0003 0.0022 0.0015 0.0019 0.003 0.0044 0.0009 0.0051 0.0007 0.0185 0.0032 0.0016 0.0009 0.0008 0.001 0.0012 0.0019
Vacuole 0.0035 0.0004 0.0015 0.0032 0.0011 0.0017 0.0083 0.0045 0.0016 0.0273 0.0169 0.0007 0.0017 0.0003 0.0007 0.0012 0.0007 0.0008
Vacuole Periphery 0.0014 0.0001 0.0004 0.0016 0.0013 0.0005 0.0014 0.0029 0.0005 0.0156 0.0023 0.0002 0.0003 0 0.0002 0.0003 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4538 16.5778 21.5275 14.3699 20.0366 16.9898 12.2221 4.7138 14.5172 11.5024
Translational Efficiency 1.743 1.6408 1.2755 2.6417 1.5504 1.6187 1.546 4.7408 1.7628 1.828

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1575 1297 388 1669 1526 2146 184 1573 3101 3443 572 3242

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 746.99 1039.51 2004.36 1344.39 856.05 1180.47 1253.78 1515.07 800.66 1127.37 1762.91 1427.20
Standard Deviation 102.29 228.21 537.86 458.63 149.19 238.11 271.93 593.32 138.71 244.18 585.62 535.13
Intensity Change Log 2 0.476743 1.423981 0.847791 0.463594 0.550517 0.823617 0.469736 1.023238 0.834932

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000156 0.000353 0.000524 0.001980 0.000103 0.000080 0.000954 0.000191 0.000130 0.000183 0.000662 0.001112
Bud Neck 0.007999 0.001175 0.003291 0.006778 0.001722 0.002146 0.002529 0.001112 0.004910 0.001780 0.003046 0.004029
Bud Site 0.006905 0.006387 0.004462 0.022059 0.002854 0.005086 0.005778 0.006414 0.004911 0.005576 0.004885 0.014468
Cell Periphery 0.000130 0.000085 0.000544 0.000236 0.000097 0.000036 0.000305 0.000154 0.000114 0.000054 0.000467 0.000196
Cytoplasm 0.606436* 0.855904* 0.542449* 0.654175* 0.765691* 0.875340* 0.375464* 0.766212* 0.684805* 0.868019* 0.488733* 0.708535*
Cytoplasmic Foci 0.196131* 0.027991 0.016629 0.009459 0.083610 0.019242 0.012512 0.004924 0.140760 0.022538 0.015305 0.007259
Eisosomes 0.000086 0.000008 0.000006 0.000012 0.000030 0.000004 0.000079 0.000003 0.000058 0.000005 0.000030 0.000007
Endoplasmic Reticulum 0.001188 0.000770 0.000554 0.000737 0.000884 0.000319 0.007954 0.000279 0.001038 0.000489 0.002935 0.000515
Endosome 0.008218 0.002539 0.000740 0.002133 0.004861 0.000909 0.006228 0.000573 0.006566 0.001523 0.002505 0.001376
Golgi 0.002321 0.001447 0.000539 0.001994 0.000508 0.000429 0.000275 0.001762 0.001429 0.000812 0.000454 0.001881
Lipid Particles 0.004421 0.001270 0.001573 0.000706 0.001352 0.000152 0.003355 0.000284 0.002910 0.000573 0.002147 0.000501
Mitochondria 0.003015 0.001495 0.000441 0.001032 0.001352 0.000533 0.000322 0.000262 0.002196 0.000895 0.000403 0.000659
Mitotic Spindle 0.000850 0.001802 0.007591 0.053511 0.003175 0.005281 0.003084 0.012838 0.001994 0.003970 0.006141 0.033777
None 0.004181 0.008101 0.012354 0.010947 0.006136 0.013295 0.003159 0.017740 0.005143 0.011339 0.009396 0.014243
Nuclear Periphery 0.000276 0.000209 0.000769 0.000600 0.000204 0.000063 0.001898 0.000206 0.000241 0.000118 0.001132 0.000409
Nuclear Periphery Foci 0.000694 0.002974 0.003851 0.001222 0.002265 0.000445 0.013114 0.000942 0.001467 0.001398 0.006830 0.001086
Nucleolus 0.000667 0.000460 0.000191 0.000278 0.000374 0.000169 0.000325 0.000191 0.000523 0.000278 0.000234 0.000236
Nucleus 0.099397 0.062501 0.374679* 0.187510* 0.081800 0.061402 0.315050* 0.169761* 0.090738 0.061816 0.355498* 0.178898*
Peroxisomes 0.002382 0.000986 0.000057 0.002222 0.000936 0.000638 0.000272 0.000054 0.001670 0.000769 0.000126 0.001170
Vacuole 0.053406 0.022710 0.028614 0.040529 0.041749 0.014242 0.247107* 0.015753 0.047669 0.017432 0.098899 0.028508
Vacuole Periphery 0.001142 0.000834 0.000141 0.001881 0.000299 0.000191 0.000236 0.000345 0.000727 0.000433 0.000171 0.001136

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.45 -10.16 -3.02 -2.25 -0.53 1.49 -9.63 -2.08 -2.25 4.20 -1.73 -13.92 -3.58 -3.18 1.24
Bud Neck 8.98 1.19 3.40 -5.07 2.45 -0.98 -4.00 -0.99 0.41 3.92 6.77 -1.45 2.93 -3.81 4.31
Bud Site 0.53 -0.37 -3.84 -4.06 -3.53 -3.51 -6.77 -5.42 -2.07 -0.15 -1.12 -3.63 -5.89 -4.79 -2.94
Cell Periphery 3.32 -9.62 -2.73 -4.81 8.69 12.15 -7.09 -6.42 -11.81 3.63 8.88 -10.41 -5.43 -10.47 9.05
Cytoplasm -29.65 11.63 2.94 35.26 -9.88 -17.31 25.91 7.41 26.92 -22.79 -34.21 25.79 6.77 45.02 -22.23
Cytoplasmic Foci 31.59* 30.21 37.62 10.54 8.14 19.57 23.10 24.17 9.28 0.63 36.07 33.31 42.34 14.19 7.84
Eisosomes 14.70 13.82 13.47 -6.09 -1.85 16.03 -6.47 14.88 -4.21 10.89 18.67 7.63 16.92 -8.90 8.90
Endoplasmic Reticulum 2.09 2.71 3.53 0.45 0.94 2.51 -8.23 6.32 0.23 9.31 3.38 -5.86 6.43 0.01 8.55
Endosome 7.77 10.99 11.51 3.45 1.73 6.94 0.64 7.76 2.24 7.80 11.21 9.35 13.74 3.77 7.38
Golgi 1.60 4.93 4.83 1.38 1.08 0.67 2.81 -0.69 -0.87 -1.21 2.53 4.78 2.53 0.23 -0.23
Lipid Particles 5.50 1.08 8.14 -0.09 6.71 11.25 -5.43 3.14 -6.71 7.00 8.95 -1.87 8.64 -2.89 8.84
Mitochondria 1.71 4.20 4.06 0.99 -1.08 1.16 1.38 1.57 1.49 3.64 2.48 3.76 3.87 1.27 1.18
Mitotic Spindle -1.65 -5.85 -11.01 -10.62 -7.11 -1.47 -0.24 -5.38 -4.30 -5.78 -2.30 -4.84 -12.08 -10.69 -8.00
None -4.22 -5.12 -9.18 -3.09 -1.67 -6.79 4.16 -6.46 0.28 -9.98 -8.42 -2.80 -10.45 -0.47 -6.34
Nuclear Periphery 0.81 -12.97 -15.62 -8.59 5.78 5.52 -15.40 -6.67 -17.13 13.87 3.17 -19.22 -16.54 -15.47 13.31
Nuclear Periphery Foci -7.49 -8.93 -7.59 4.58 7.37 9.75 -7.54 3.81 -7.87 8.48 -0.03 -10.97 -0.67 -0.50 10.88
Nucleolus 1.15 3.59 3.25 1.01 -1.05 3.89 1.73 3.32 -0.44 2.39 2.85 4.09 4.34 0.63 1.07
Nucleus 8.67 -25.95 -22.31 -30.82 14.78 6.18 -13.89 -22.21 -28.27 7.20 10.96 -29.35 -31.32 -42.32 16.71
Peroxisomes 4.59 12.01 4.91 0.69 -1.99 0.90 2.76 3.33 3.13 5.92 4.16 9.53 5.86 1.78 -1.62
Vacuole 9.51 -3.90 -14.38 -20.17 -5.92 11.42 -14.42 -3.46 -12.72 13.70 15.68 -12.06 -13.67 -24.07 6.95
Vacuole Periphery 1.11 5.61 2.17 0.69 -1.50 2.61 2.68 1.19 -0.05 0.23 2.29 6.00 2.39 0.26 -1.34