Standard name
Description Methylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Cytoplasm 0.88 0.99 0.99 0.92 0.85 0.85 0.71 – 0.73 0.68 0.67 0.68 0.99 0.99 0.97 0.94 0.94 0.89 0.83 0.89 0.95 0.9 0.89 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0 – 0.07 0.06 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0.09 0.19 0.09 0.3 – 0.2 0.22 0.24 0.18 0 0 0 0.09 0.09 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 1 3 1 0 0 0 0 0 0 1 1 1 0 1 3
Bud 0 3 0 0 0 2 0 – 0 0 1 4 0 0 0 0 0 0 1 3 1 13 7 18
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 3 2 7 1 6 – 7 4 8 9 2 2 8 9 3 0 0 0 0 0 0 0
Cytoplasm 92 232 286 197 205 169 233 – 157 149 157 173 287 325 351 102 44 16 120 212 276 295 272 260
Endoplasmic Reticulum 0 0 0 3 1 0 3 – 8 6 1 13 0 0 1 8 0 0 1 0 0 2 3 4
Endosome 1 0 0 0 1 0 0 – 0 0 1 0 0 0 1 0 0 0 0 5 0 1 1 4
Golgi 0 0 2 0 0 0 0 – 0 2 3 1 0 0 0 0 0 0 5 0 2 1 1 1
Mitochondria 4 0 0 3 3 24 13 – 15 14 20 16 1 0 1 2 0 0 1 2 1 1 2 3
Nucleus 1 1 2 0 1 3 5 – 1 5 5 4 1 3 2 0 0 0 1 5 1 0 0 1
Nuclear Periphery 2 0 0 0 0 1 0 – 0 4 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 1 0 2 2 2 1
Vac/Vac Membrane 6 4 0 19 46 17 99 – 42 48 55 46 1 1 7 10 4 2 4 2 0 5 8 5
Unique Cell Count 104 235 289 214 241 199 327 214 219 233 254 289 327 360 108 47 18 145 239 290 328 306 316
Labelled Cell Count 107 242 293 224 264 217 360 231 233 254 267 292 331 371 131 51 18 145 239 290 328 306 316


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.8 7.9 7.9 9.0 7.4 10.1 – 12.7 12.3 14.3 15.3 10.6 12.7 13.8 8.0 8.3 7.5 8.1 8.2 9.3
Std Deviation (1e-4) 1.7 2.0 1.7 2.0 2.3 2.7 2.9 – 5.2 4.5 6.0 6.1 3.4 5.0 6.1 3.1 2.4 1.7 6.9 6.4 7.0
Intensity Change (Log2) – – – 0.01 0.19 -0.09 0.36 – 0.69 0.65 0.86 0.96 0.43 0.69 0.81 0.03 0.08 -0.07 0.05 0.06 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 2.0 0 0 0 0 0 0
Cytoplasm -3.9 -6.1 -6.1 -9.4 – -8.8 -9.8 -10.0 -9.9 0.4 0.6 -1.4 -2.7 -2.6 -3.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 3.9 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.1 3.4 – 4.6 4.4 5.1 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 5.2 7.8 5.1 10.2 – 7.9 8.4 8.7 7.6 0 0 0 0 0 0