Standard name | |
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Description | Methylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 3 |
Bud | 0 | 3 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 13 | 7 | 18 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 3 | 2 | 7 | 1 | 6 | – | 7 | 4 | 8 | 9 | 2 | 2 | 8 | 9 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 92 | 232 | 286 | 197 | 205 | 169 | 233 | – | 157 | 149 | 157 | 173 | 287 | 325 | 351 | 102 | 44 | 16 | 120 | 212 | 276 | 295 | 272 | 260 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3 | 1 | 0 | 3 | – | 8 | 6 | 1 | 13 | 0 | 0 | 1 | 8 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 4 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 5 | 0 | 1 | 1 | 4 |
Golgi | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 2 | 1 | 1 | 1 |
Mitochondria | 4 | 0 | 0 | 3 | 3 | 24 | 13 | – | 15 | 14 | 20 | 16 | 1 | 0 | 1 | 2 | 0 | 0 | 1 | 2 | 1 | 1 | 2 | 3 |
Nucleus | 1 | 1 | 2 | 0 | 1 | 3 | 5 | – | 1 | 5 | 5 | 4 | 1 | 3 | 2 | 0 | 0 | 0 | 1 | 5 | 1 | 0 | 0 | 1 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 1 |
Vac/Vac Membrane | 6 | 4 | 0 | 19 | 46 | 17 | 99 | – | 42 | 48 | 55 | 46 | 1 | 1 | 7 | 10 | 4 | 2 | 4 | 2 | 0 | 5 | 8 | 5 |
Unique Cell Count | 104 | 235 | 289 | 214 | 241 | 199 | 327 | 214 | 219 | 233 | 254 | 289 | 327 | 360 | 108 | 47 | 18 | 145 | 239 | 290 | 328 | 306 | 316 | |
Labelled Cell Count | 107 | 242 | 293 | 224 | 264 | 217 | 360 | 231 | 233 | 254 | 267 | 292 | 331 | 371 | 131 | 51 | 18 | 145 | 239 | 290 | 328 | 306 | 316 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 7.8 | 7.9 | 7.9 | 9.0 | 7.4 | 10.1 | – | 12.7 | 12.3 | 14.3 | 15.3 | 10.6 | 12.7 | 13.8 | 8.0 | 8.3 | 7.5 | 8.1 | 8.2 | 9.3 |
Std Deviation (1e-4) | 1.7 | 2.0 | 1.7 | 2.0 | 2.3 | 2.7 | 2.9 | – | 5.2 | 4.5 | 6.0 | 6.1 | 3.4 | 5.0 | 6.1 | 3.1 | 2.4 | 1.7 | 6.9 | 6.4 | 7.0 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.19 | -0.09 | 0.36 | – | 0.69 | 0.65 | 0.86 | 0.96 | 0.43 | 0.69 | 0.81 | 0.03 | 0.08 | -0.07 | 0.05 | 0.06 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.9 | -6.1 | -6.1 | -9.4 | – | -8.8 | -9.8 | -10.0 | -9.9 | 0.4 | 0.6 | -1.4 | -2.7 | -2.6 | -3.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.1 | 3.4 | – | 4.6 | 4.4 | 5.1 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 5.2 | 7.8 | 5.1 | 10.2 | – | 7.9 | 8.4 | 8.7 | 7.6 | 0 | 0 | 0 | 0 | 0 | 0 |