Standard name
Human Ortholog
Description Cytosolic branched-chain amino acid (BCAA) aminotransferase; preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase; BAT2 has a paralog, BAT1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.99 0.99 0.98 0.93 0.92 0.94 0.89 0.91 0.9 0.99 1.0 1.0 0.99 0.99 0.97 0.97 0.97 0.95 0.94 0.96 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.07 0.05 0.05 0.07 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 0 0 0 1 0 0 1 1 4 1 0 0 0 0 0 0 0 0 0 1 6 6 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 0 0 4 6 8 8 9 8 8 13 2 2 2 5 4 3 0 0 0 0 0 0
Cytoplasm 345 136 312 271 415 354 368 359 334 280 290 267 408 497 502 237 291 238 300 129 277 315 540 474
Endoplasmic Reticulum 1 0 4 1 5 8 19 27 19 15 22 19 1 2 0 5 15 9 0 0 1 0 0 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 2 0 0 1 0 0 1 0 0 1 0 0 0 2 2 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0
Nucleus 0 1 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 3 3 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 1 0 0 0 1 0 0 0 1 1 0 0 0 1 1 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 1 1 0 1 9 0 1 0 0 1 4 1 3 1 0 1 2 3 4
Unique Cell Count 346 137 317 274 420 362 395 391 356 314 318 296 411 499 503 240 294 245 311 133 293 337 562 496
Labelled Cell Count 347 137 318 274 426 369 398 397 364 319 323 303 413 501 505 252 314 258 311 133 293 337 562 496


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 108.2 147.2 110.2 104.3 131.5 123.6 111.4 115.7 106.7 96.3 96.3 100.4 116.3 123.3 121.9 142.7 144.8 143.4 118.8 124.4 128.6
Std Deviation (1e-4) 24.4 36.3 26.8 26.9 29.9 28.5 28.8 29.6 27.9 33.0 25.8 27.6 30.4 32.3 31.9 35.7 46.6 45.4 32.4 32.0 35.4
Intensity Change (Log2) -0.08 0.25 0.17 0.02 0.07 -0.05 -0.2 -0.2 -0.13 0.08 0.16 0.15 0.37 0.39 0.38 0.11 0.17 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 3.8 0 0 0 0 0 0
Cytoplasm 0.5 0.4 -0.6 -3.4 -3.9 -3.0 -4.8 -4.1 -4.4 1.1 1.8 2.3 0.3 0.6 -1.0
Endoplasmic Reticulum 0 0 0.9 2.7 3.6 2.9 2.6 3.6 3.4 0 0 0 0 2.7 1.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.3739 48.8177 47.744 43.2678 39.0782 42.0081 29.7293 40.4196 37.643 35.7399 34.6637 38.2079 37.4679 57.1684 47.7653 42.3211 62.5145 48.5474
Actin 0.0016 0.0007 0.0011 0 0.0045 0.0003 0.0083 0.0005 0.0021 0.0007 0.0075 0.0006 0.0048 0.0001 0.0016 0 0.0001 0.001
Bud 0.0007 0.0005 0.0001 0.0001 0.001 0.0001 0.0008 0.0002 0.0008 0.0002 0.0004 0.0002 0.0011 0.0006 0.0008 0.0001 0 0.0003
Bud Neck 0.0004 0.0001 0.0002 0.0002 0.0005 0.0004 0.0005 0.0001 0.0005 0.0004 0.0005 0.0006 0.0003 0.0001 0.0002 0.0001 0.0001 0.0007
Bud Periphery 0.0006 0.0001 0.0001 0 0.0008 0 0.0006 0 0.0005 0.0002 0.0004 0.0001 0.0007 0.0001 0.0003 0 0 0.0003
Bud Site 0.0004 0.0004 0.0003 0 0.0007 0.0001 0.001 0.0001 0.0035 0.0002 0.0007 0 0.0009 0.0002 0.0007 0 0 0
Cell Periphery 0.0003 0.0001 0.0001 0 0.0013 0 0.0004 0 0.0003 0.0002 0.0004 0 0.0002 0 0.0001 0 0 0
Cytoplasm 0.9388 0.9896 0.9695 0.9855 0.8578 0.9636 0.8902 0.9897 0.9609 0.9661 0.8847 0.97 0.9187 0.9947 0.9752 0.9944 0.9925 0.9713
Cytoplasmic Foci 0.0039 0.0007 0.0044 0.0005 0.0092 0.0009 0.0085 0.0004 0.003 0.0007 0.0071 0.0005 0.0016 0.0003 0.002 0.0003 0.0002 0.0007
Eisosomes 0 0 0 0 0.0002 0 0.0001 0 0 0 0.0004 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0019 0.0002 0.0009 0.0003 0.0038 0.0006 0.0052 0.0003 0.0013 0.006 0.0057 0.0012 0.0038 0.0001 0.0006 0.0001 0.0002 0.0045
Endosome 0.0024 0.0001 0.001 0.0003 0.0119 0.0002 0.0083 0.0001 0.0015 0.0005 0.0085 0.0002 0.0139 0 0.0026 0.0001 0 0.0046
Golgi 0.0007 0 0.0003 0 0.0018 0 0.0025 0 0.0004 0.0001 0.0029 0 0.0032 0 0.0005 0 0 0.0006
Lipid Particles 0.0058 0 0.0019 0 0.005 0 0.0098 0 0.0009 0.0002 0.0082 0 0.0016 0 0 0 0 0
Mitochondria 0.0028 0.0001 0.0033 0 0.0151 0.0003 0.0063 0.0001 0.0005 0.0002 0.022 0.0003 0.0091 0 0.0051 0 0 0.0016
None 0.002 0.0004 0.0007 0.0002 0.0124 0.0013 0.0028 0.0003 0.0015 0.0011 0.0052 0.0003 0.0019 0.0003 0.0006 0.0006 0.0002 0.0003
Nuclear Periphery 0.0043 0.0008 0.0024 0.0012 0.0101 0.0034 0.009 0.0013 0.0027 0.0053 0.0074 0.0036 0.0125 0.0005 0.0019 0.0006 0.0009 0.0041
Nucleolus 0.0023 0 0.0002 0 0.0078 0.0038 0.0017 0 0.0007 0.0001 0.0013 0 0.0005 0 0.0001 0 0 0
Nucleus 0.0202 0.0055 0.0098 0.0104 0.0261 0.023 0.0274 0.0066 0.0132 0.0158 0.021 0.0213 0.0146 0.0026 0.0053 0.003 0.0055 0.0076
Peroxisomes 0.004 0 0.0016 0 0.0009 0 0.0035 0 0.0002 0 0.0028 0 0.0003 0 0.0001 0 0 0
Punctate Nuclear 0.0012 0.0001 0.0011 0.0001 0.002 0.0007 0.0049 0.0001 0.0009 0.0005 0.0028 0.0004 0.0005 0.0001 0.0002 0.0001 0.0002 0.0002
Vacuole 0.0036 0.0004 0.0008 0.001 0.0162 0.0008 0.0066 0.0002 0.004 0.0015 0.0086 0.0005 0.0061 0.0003 0.0015 0.0005 0.0001 0.0008
Vacuole Periphery 0.0021 0.0001 0.0003 0.0001 0.0111 0.0003 0.0015 0 0.0008 0.0002 0.0015 0.0001 0.0037 0 0.0005 0.0001 0 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 544.3852 416.0048 424.5345 446.9228 320.022 476.8047 537.0206 560.3435 589.8049 438.5941
Translational Efficiency 1.4663 1.5666 1.4389 1.6636 1.5629 1.6073 1.3603 1.3526 1.3056 1.3007

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency