Standard name
Description Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.85 0.95 0.94 0.89 0.87 0.88 0.89 0.82 0.83 0.69 0.83 0.94 0.98 0.95 0.58 0.54 0.3 0.64 0.68 0.63
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.14 0.08 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0 0.1 0.08 0.25 0.12 0.44 0.28 0 0 0 0.13 0.08 0.17 0.06 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.12 0.06 0 0.13 0.09 0.06 0 0.06 0 0 0 0 0 0 0.12 0.17 0.35 0.15 0.16 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 1 1 0 2 2 0 0 0 0 0 2 0 0 0 2
Bud 0 0 0 1 2 0 1 6 2 6 9 2 0 0 0 0 0 1 0 0 3
Bud Neck 0 0 1 1 0 2 3 4 2 6 2 2 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 1 0 0 3 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 121 149 63 215 317 296 418 425 170 272 197 334 16 83 154 87 108 30 15 88 122
Endoplasmic Reticulum 2 1 0 5 1 1 5 0 0 0 6 1 1 0 0 1 0 1 0 0 0
Endosome 1 9 0 3 8 8 11 11 2 5 0 3 0 2 2 15 27 7 0 5 10
Golgi 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0
Mitochondria 8 3 0 0 0 15 47 40 52 40 126 110 0 0 0 19 15 17 1 6 6
Nucleus 1 1 1 0 0 3 2 2 2 6 1 1 0 0 1 0 0 1 0 0 1
Nuclear Periphery 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 2 0 3 0 3 6 9 3 7 1 5 0 0 0 2 3 0 0 3 6
Vac/Vac Membrane 3 21 4 9 46 29 27 15 13 10 10 7 0 0 7 18 33 35 3 20 35
Unique Cell Count 133 175 66 229 356 340 475 479 207 326 287 400 17 85 162 152 200 100 24 130 195
Labelled Cell Count 137 187 70 240 374 359 523 514 246 357 354 466 17 85 165 152 200 100 24 130 195


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.7 5.5 5.2 5.9 4.8 4.8 5.0 4.1 4.2 3.9 4.2 4.5 5.4 5.2 4.8 5.4
Std Deviation (1e-4) 0.6 1.2 1.3 1.2 1.8 1.4 1.3 1.2 1.3 0.9 1.0 1.3 0.7 1.1 1.2 1.1 1.2
Intensity Change (Log2) -0.09 0.1 -0.2 -0.2 -0.13 -0.42 -0.38 -0.52 -0.39 -0.3 -0.04 -0.09 -0.2 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available