Standard name | |
---|---|
Description | Kinesin motor protein; involved in mitotic spindle assembly and chromosome segregation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 7 | 4 |
Bud | 1 | 1 | 2 | 3 | 3 | 7 | 12 | 18 | 5 | 7 | 6 | 10 | 1 | 1 | 3 | 0 | 0 | 1 | 9 | 9 | 2 | 7 | 6 | 5 |
Bud Neck | 3 | 2 | 4 | 1 | 4 | 6 | 9 | 14 | 7 | 6 | 4 | 8 | 1 | 1 | 3 | 3 | 1 | 1 | 1 | 3 | 0 | 0 | 2 | 2 |
Bud Site | 0 | 3 | 3 | 4 | 5 | 10 | 15 | 25 | 6 | 11 | 3 | 12 | 0 | 1 | 4 | 3 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 30 | 35 | 15 | 38 | 30 | 29 | 55 | 107 | 37 | 51 | 34 | 58 | 32 | 76 | 99 | 46 | 12 | 53 | 11 | 19 | 10 | 17 | 77 | 134 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 3 | 5 | 0 | 1 | 0 | 0 | 1 | 0 |
Endosome | 32 | 14 | 0 | 0 | 7 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 14 | 10 | 5 | 47 | 27 | 48 | 14 | 11 | 4 | 7 | 15 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 |
Mitochondria | 21 | 32 | 14 | 37 | 50 | 62 | 100 | 144 | 50 | 60 | 44 | 111 | 9 | 4 | 8 | 13 | 6 | 19 | 7 | 7 | 4 | 3 | 7 | 10 |
Nucleus | 21 | 9 | 3 | 9 | 9 | 9 | 22 | 24 | 6 | 7 | 4 | 8 | 2 | 2 | 3 | 15 | 6 | 10 | 12 | 8 | 2 | 3 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 42 | 23 | 7 | 7 | 20 | 21 | 33 | 32 | 11 | 9 | 4 | 19 | 15 | 21 | 19 | 47 | 19 | 42 | 7 | 9 | 5 | 3 | 0 | 2 |
Peroxisomes | 4 | 3 | 0 | 0 | 4 | 1 | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 6 | 10 | 17 | 7 | 18 | 1 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 73 | 43 | 21 | 26 | 80 | 91 | 84 | 138 | 39 | 54 | 30 | 47 | 28 | 65 | 58 | 157 | 56 | 148 | 109 | 86 | 46 | 59 | 50 | 30 |
Vac/Vac Membrane | 14 | 21 | 0 | 0 | 3 | 0 | 3 | 3 | 1 | 1 | 0 | 1 | 7 | 4 | 1 | 25 | 9 | 28 | 8 | 7 | 2 | 3 | 10 | 4 |
Unique Cell Count | 169 | 146 | 50 | 93 | 163 | 181 | 236 | 366 | 126 | 151 | 99 | 199 | 90 | 157 | 181 | 272 | 97 | 272 | 191 | 170 | 82 | 111 | 190 | 214 |
Labelled Cell Count | 241 | 187 | 69 | 125 | 215 | 236 | 333 | 512 | 168 | 206 | 130 | 275 | 109 | 192 | 213 | 381 | 146 | 376 | 191 | 170 | 82 | 111 | 190 | 214 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 3.9 | 3.1 | 3.2 | 3.3 | 3.1 | 2.9 | 2.9 | 2.8 | 2.4 | 2.4 | 2.3 | 4.9 | 5.2 | 4.7 | 5.8 | 6.2 | 6.6 | 4.9 | 4.7 | 4.8 |
Std Deviation (1e-4) | 0.8 | 0.7 | 0.3 | 1.2 | 0.6 | 0.7 | 0.8 | 0.9 | 1.0 | 0.5 | 0.4 | 0.6 | 2.1 | 2.0 | 1.9 | 1.7 | 2.4 | 2.0 | 2.4 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.12 | 0.04 | -0.07 | -0.06 | -0.15 | -0.35 | -0.32 | -0.43 | 0.69 | 0.76 | 0.61 | 0.92 | 1.02 | 1.1 | 0.68 | 0.6 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2388 | 0.9262 | 1.3612 | 1.2936 | 0.9852 | 1.0214 | 1.5369 | 2.014 | 2.3332 | 2.5048 | 2.2162 | 1.9771 | -1.2378 | 0.1177 | 0.1415 | 0.3362 | 0.2497 | -0.3591 |
Actin | 0.0353 | 0.0007 | 0.0149 | 0.0033 | 0.0324 | 0.0032 | 0.056 | 0.0072 | 0.0016 | 0.0227 | 0.0014 | 0.0019 | 0.0207 | 0.0001 | 0.0003 | 0.0005 | 0.0003 | 0.0004 |
Bud | 0.0024 | 0.0006 | 0.0006 | 0.0002 | 0.0009 | 0.0005 | 0.0012 | 0.0008 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 |
Bud Neck | 0.0092 | 0.0015 | 0.0028 | 0.0008 | 0.048 | 0.013 | 0.005 | 0.0091 | 0.0005 | 0.0183 | 0.0034 | 0.0158 | 0.001 | 0.0008 | 0.0005 | 0.0083 | 0.0075 | 0.0062 |
Bud Periphery | 0.0055 | 0.0011 | 0.0013 | 0.0007 | 0.0036 | 0.0019 | 0.0018 | 0.0012 | 0.0002 | 0.0002 | 0.0011 | 0.0011 | 0.001 | 0.0001 | 0 | 0 | 0.0006 | 0.0003 |
Bud Site | 0.0173 | 0.0112 | 0.0027 | 0.0004 | 0.0061 | 0.001 | 0.0134 | 0.004 | 0.001 | 0.0017 | 0.0014 | 0.0003 | 0.0024 | 0.0009 | 0.0002 | 0.0001 | 0.0008 | 0.0002 |
Cell Periphery | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0012 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.0377 | 0.0077 | 0.0126 | 0.0005 | 0.0009 | 0.0126 | 0.0092 | 0.004 | 0.001 | 0.0006 | 0.0017 | 0.003 | 0.0524 | 0.0052 | 0.0019 | 0.0004 | 0.0032 | 0.001 |
Cytoplasmic Foci | 0.0909 | 0.0716 | 0.0479 | 0.0556 | 0.0395 | 0.042 | 0.0699 | 0.0378 | 0.0244 | 0.043 | 0.0064 | 0.0159 | 0.0953 | 0.0391 | 0.0475 | 0.0661 | 0.0023 | 0.0539 |
Eisosomes | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0016 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0021 | 0.0001 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0071 | 0.0003 | 0.0028 | 0.0012 | 0.0036 | 0.0022 | 0.0038 | 0.0003 | 0.0011 | 0.0002 | 0.0005 | 0.0006 | 0.0027 | 0.001 | 0.0001 | 0.0001 | 0.0005 | 0.0002 |
Endosome | 0.0386 | 0.0028 | 0.0016 | 0.0093 | 0.0176 | 0.008 | 0.0175 | 0.0012 | 0.0089 | 0.0115 | 0.0145 | 0.009 | 0.0075 | 0.0011 | 0.0013 | 0.0051 | 0.0041 | 0.002 |
Golgi | 0.0205 | 0.0006 | 0.0015 | 0.0044 | 0.0094 | 0.0037 | 0.0149 | 0.0005 | 0.0047 | 0.0062 | 0.0055 | 0.0049 | 0.003 | 0.0012 | 0.0005 | 0.0022 | 0.0003 | 0.0008 |
Lipid Particles | 0.0627 | 0.0082 | 0.0096 | 0.0364 | 0.0601 | 0.0202 | 0.0208 | 0.004 | 0.007 | 0.0097 | 0.0165 | 0.0123 | 0.0159 | 0.0061 | 0.0073 | 0.0211 | 0.0031 | 0.0112 |
Mitochondria | 0.0259 | 0.0009 | 0.0165 | 0.0125 | 0.0381 | 0.0147 | 0.0062 | 0.0004 | 0.0118 | 0.0006 | 0.0433 | 0.0014 | 0.002 | 0.0002 | 0.0001 | 0.0002 | 0.0009 | 0.0028 |
None | 0.0463 | 0.0807 | 0.0006 | 0.0004 | 0.003 | 0.0047 | 0.0447 | 0.0456 | 0.0008 | 0.0004 | 0.0023 | 0.0067 | 0.2131 | 0.2113 | 0.0064 | 0.0006 | 0.0041 | 0.0017 |
Nuclear Periphery | 0.0491 | 0.0013 | 0.0049 | 0.0078 | 0.0542 | 0.024 | 0.0065 | 0.0015 | 0.0011 | 0.0016 | 0.0347 | 0.0042 | 0.0085 | 0.0007 | 0.003 | 0.0057 | 0.0058 | 0.0065 |
Nucleolus | 0.0152 | 0.0405 | 0.0433 | 0.029 | 0.1606 | 0.077 | 0.025 | 0.1078 | 0.0395 | 0.0369 | 0.1886 | 0.0837 | 0.0132 | 0.0478 | 0.0131 | 0.0027 | 0.1113 | 0.0379 |
Nucleus | 0.1048 | 0.016 | 0.0168 | 0.0046 | 0.1661 | 0.1483 | 0.0229 | 0.0656 | 0.0302 | 0.06 | 0.464 | 0.1457 | 0.055 | 0.0587 | 0.01 | 0.0009 | 0.4091 | 0.1128 |
Peroxisomes | 0.0198 | 0.0028 | 0.0044 | 0.0059 | 0.0116 | 0.0035 | 0.0084 | 0.0027 | 0.0048 | 0.0046 | 0.0035 | 0.0066 | 0.0033 | 0.002 | 0.0012 | 0.0074 | 0.0005 | 0.0016 |
Punctate Nuclear | 0.4018 | 0.7503 | 0.8131 | 0.8245 | 0.3329 | 0.6151 | 0.6689 | 0.7043 | 0.8604 | 0.7812 | 0.2067 | 0.6845 | 0.4977 | 0.6233 | 0.9066 | 0.8785 | 0.4421 | 0.7597 |
Vacuole | 0.0047 | 0.0007 | 0.0012 | 0.001 | 0.0048 | 0.0024 | 0.0022 | 0.0014 | 0.0006 | 0.0002 | 0.0022 | 0.0015 | 0.0019 | 0.0003 | 0.0001 | 0.0001 | 0.0026 | 0.0005 |
Vacuole Periphery | 0.0042 | 0.0001 | 0.0003 | 0.0012 | 0.0039 | 0.0017 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0015 | 0.0006 | 0.0008 | 0 | 0 | 0.0001 | 0.0005 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.5972 | 21.3501 | 5.8496 | 5.6283 | 22.1523 | 15.4237 | 19.2623 | 15.0181 | 7.9564 | 24.1253 |
Translational Efficiency | 0.6572 | 0.7233 | 0.4943 | 0.4775 | 0.53 | 0.843 | 0.5983 | 0.4495 | 0.5718 | 0.555 |
Dataset | Hit |
---|---|
Protein Abundance | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |