Standard name
Description Kinesin motor protein; involved in mitotic spindle assembly and chromosome segregation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.05 0 0.05 0.06 0.05 0 0 0 0 0 0 0.05 0.05 0 0.07 0 0
Bud Neck 0 0 0.08 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0.06 0 0 0.06 0.06 0.07 0.05 0.07 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.24 0.3 0.41 0.18 0.16 0.23 0.29 0.29 0.34 0.34 0.29 0.36 0.48 0.55 0.17 0.12 0.19 0.06 0.12 0.13 0.16 0.41 0.63
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.19 0.1 0 0 0 0 0 0 0 0 0 0 0.16 0.06 0 0.17 0.28 0.18 0.08 0.07 0.05 0.07 0.08 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.22 0.28 0.4 0.31 0.34 0.42 0.39 0.4 0.4 0.44 0.56 0.1 0 0 0.05 0.06 0.07 0 0 0.05 0 0 0.05
Nucleus 0.12 0.06 0.06 0.1 0.06 0.05 0.09 0.07 0.05 0.05 0 0 0 0 0 0.06 0.06 0 0.07 0.05 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.25 0.16 0.14 0.08 0.12 0.12 0.14 0.09 0.09 0.06 0 0.1 0.17 0.13 0.1 0.17 0.2 0.15 0 0.06 0.07 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0.07 0 0 0 0 0 0
SpindlePole 0.43 0.29 0.42 0.28 0.49 0.5 0.36 0.38 0.31 0.36 0.3 0.24 0.31 0.41 0.32 0.58 0.58 0.54 0.58 0.51 0.57 0.53 0.26 0.14
Vac/Vac Membrane 0.08 0.14 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.09 0.09 0.1 0 0 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 7 4
Bud 1 1 2 3 3 7 12 18 5 7 6 10 1 1 3 0 0 1 9 9 2 7 6 5
Bud Neck 3 2 4 1 4 6 9 14 7 6 4 8 1 1 3 3 1 1 1 3 0 0 2 2
Bud Site 0 3 3 4 5 10 15 25 6 11 3 12 0 1 4 3 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 30 35 15 38 30 29 55 107 37 51 34 58 32 76 99 46 12 53 11 19 10 17 77 134
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 3 5 0 1 0 0 1 0
Endosome 32 14 0 0 7 0 0 1 2 0 0 0 14 10 5 47 27 48 14 11 4 7 15 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 2 2
Mitochondria 21 32 14 37 50 62 100 144 50 60 44 111 9 4 8 13 6 19 7 7 4 3 7 10
Nucleus 21 9 3 9 9 9 22 24 6 7 4 8 2 2 3 15 6 10 12 8 2 3 1 0
Nuclear Periphery 0 1 0 0 0 0 0 1 1 0 0 1 0 1 0 0 0 1 0 0 0 0 0 1
Nucleolus 42 23 7 7 20 21 33 32 11 9 4 19 15 21 19 47 19 42 7 9 5 3 0 2
Peroxisomes 4 3 0 0 4 1 0 5 2 0 0 0 0 6 10 17 7 18 1 0 0 0 1 0
SpindlePole 73 43 21 26 80 91 84 138 39 54 30 47 28 65 58 157 56 148 109 86 46 59 50 30
Vac/Vac Membrane 14 21 0 0 3 0 3 3 1 1 0 1 7 4 1 25 9 28 8 7 2 3 10 4
Unique Cell Count 169 146 50 93 163 181 236 366 126 151 99 199 90 157 181 272 97 272 191 170 82 111 190 214
Labelled Cell Count 241 187 69 125 215 236 333 512 168 206 130 275 109 192 213 381 146 376 191 170 82 111 190 214


Microtubule, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 3.9 3.1 3.2 3.3 3.1 2.9 2.9 2.8 2.4 2.4 2.3 4.9 5.2 4.7 5.8 6.2 6.6 4.9 4.7 4.8
Std Deviation (1e-4) 0.8 0.7 0.3 1.2 0.6 0.7 0.8 0.9 1.0 0.5 0.4 0.6 2.1 2.0 1.9 1.7 2.4 2.0 2.4 1.9 2.2
Intensity Change (Log2) – – – 0.04 0.12 0.04 -0.07 -0.06 -0.15 -0.35 -0.32 -0.43 0.69 0.76 0.61 0.92 1.02 1.1 0.68 0.6 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2388 0.9262 1.3612 1.2936 0.9852 1.0214 1.5369 2.014 2.3332 2.5048 2.2162 1.9771 -1.2378 0.1177 0.1415 0.3362 0.2497 -0.3591
Actin 0.0353 0.0007 0.0149 0.0033 0.0324 0.0032 0.056 0.0072 0.0016 0.0227 0.0014 0.0019 0.0207 0.0001 0.0003 0.0005 0.0003 0.0004
Bud 0.0024 0.0006 0.0006 0.0002 0.0009 0.0005 0.0012 0.0008 0.0001 0.0001 0.0005 0.0002 0.0003 0.0001 0 0 0.0002 0.0001
Bud Neck 0.0092 0.0015 0.0028 0.0008 0.048 0.013 0.005 0.0091 0.0005 0.0183 0.0034 0.0158 0.001 0.0008 0.0005 0.0083 0.0075 0.0062
Bud Periphery 0.0055 0.0011 0.0013 0.0007 0.0036 0.0019 0.0018 0.0012 0.0002 0.0002 0.0011 0.0011 0.001 0.0001 0 0 0.0006 0.0003
Bud Site 0.0173 0.0112 0.0027 0.0004 0.0061 0.001 0.0134 0.004 0.001 0.0017 0.0014 0.0003 0.0024 0.0009 0.0002 0.0001 0.0008 0.0002
Cell Periphery 0.0007 0.0001 0.0002 0.0001 0.0012 0.0002 0.0005 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0 0 0.0002 0.0001
Cytoplasm 0.0377 0.0077 0.0126 0.0005 0.0009 0.0126 0.0092 0.004 0.001 0.0006 0.0017 0.003 0.0524 0.0052 0.0019 0.0004 0.0032 0.001
Cytoplasmic Foci 0.0909 0.0716 0.0479 0.0556 0.0395 0.042 0.0699 0.0378 0.0244 0.043 0.0064 0.0159 0.0953 0.0391 0.0475 0.0661 0.0023 0.0539
Eisosomes 0.0005 0.0001 0.0003 0.0001 0.0016 0.0001 0.0005 0 0.0001 0.0001 0.0001 0 0.0021 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0071 0.0003 0.0028 0.0012 0.0036 0.0022 0.0038 0.0003 0.0011 0.0002 0.0005 0.0006 0.0027 0.001 0.0001 0.0001 0.0005 0.0002
Endosome 0.0386 0.0028 0.0016 0.0093 0.0176 0.008 0.0175 0.0012 0.0089 0.0115 0.0145 0.009 0.0075 0.0011 0.0013 0.0051 0.0041 0.002
Golgi 0.0205 0.0006 0.0015 0.0044 0.0094 0.0037 0.0149 0.0005 0.0047 0.0062 0.0055 0.0049 0.003 0.0012 0.0005 0.0022 0.0003 0.0008
Lipid Particles 0.0627 0.0082 0.0096 0.0364 0.0601 0.0202 0.0208 0.004 0.007 0.0097 0.0165 0.0123 0.0159 0.0061 0.0073 0.0211 0.0031 0.0112
Mitochondria 0.0259 0.0009 0.0165 0.0125 0.0381 0.0147 0.0062 0.0004 0.0118 0.0006 0.0433 0.0014 0.002 0.0002 0.0001 0.0002 0.0009 0.0028
None 0.0463 0.0807 0.0006 0.0004 0.003 0.0047 0.0447 0.0456 0.0008 0.0004 0.0023 0.0067 0.2131 0.2113 0.0064 0.0006 0.0041 0.0017
Nuclear Periphery 0.0491 0.0013 0.0049 0.0078 0.0542 0.024 0.0065 0.0015 0.0011 0.0016 0.0347 0.0042 0.0085 0.0007 0.003 0.0057 0.0058 0.0065
Nucleolus 0.0152 0.0405 0.0433 0.029 0.1606 0.077 0.025 0.1078 0.0395 0.0369 0.1886 0.0837 0.0132 0.0478 0.0131 0.0027 0.1113 0.0379
Nucleus 0.1048 0.016 0.0168 0.0046 0.1661 0.1483 0.0229 0.0656 0.0302 0.06 0.464 0.1457 0.055 0.0587 0.01 0.0009 0.4091 0.1128
Peroxisomes 0.0198 0.0028 0.0044 0.0059 0.0116 0.0035 0.0084 0.0027 0.0048 0.0046 0.0035 0.0066 0.0033 0.002 0.0012 0.0074 0.0005 0.0016
Punctate Nuclear 0.4018 0.7503 0.8131 0.8245 0.3329 0.6151 0.6689 0.7043 0.8604 0.7812 0.2067 0.6845 0.4977 0.6233 0.9066 0.8785 0.4421 0.7597
Vacuole 0.0047 0.0007 0.0012 0.001 0.0048 0.0024 0.0022 0.0014 0.0006 0.0002 0.0022 0.0015 0.0019 0.0003 0.0001 0.0001 0.0026 0.0005
Vacuole Periphery 0.0042 0.0001 0.0003 0.0012 0.0039 0.0017 0.0007 0.0002 0.0002 0.0001 0.0015 0.0006 0.0008 0 0 0.0001 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.5972 21.3501 5.8496 5.6283 22.1523 15.4237 19.2623 15.0181 7.9564 24.1253
Translational Efficiency 0.6572 0.7233 0.4943 0.4775 0.53 0.843 0.5983 0.4495 0.5718 0.555

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘