Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0.07 0.06 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.99 0.96 0.9 – 0.71 0.77 0.63 0.67 0.65 0.94 0.98 0.95 – – – 0.98 0.93 0.9 0.9 0.87 0.83
Endoplasmic Reticulum 0 0 0 0 0 0.09 – 0.19 0.14 0.26 0.14 0.22 0 0 0 – – – 0 0 0.06 0.05 0.06 0.07
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0.06 0 0 0.05 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0.07 0.12 0.09 0.25 0.18 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 3 – 8 5 7 7 6 0 0 0 – – – 0 0 0 0 0 1
Bud 0 0 0 0 0 0 – 1 0 0 0 0 0 1 2 – – – 1 0 0 1 2 4
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 2 2 2 2 3 4 – 8 6 13 11 9 5 4 10 – – – 0 0 1 0 0 0
Cytoplasm 257 316 222 208 174 209 – 175 153 118 128 145 198 191 293 – – – 237 288 208 165 154 156
Endoplasmic Reticulum 2 10 7 3 6 20 – 46 27 48 26 50 8 6 9 – – – 0 8 12 8 9 12
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 1 0 0 0 1 1 – 15 4 8 9 4 0 0 0 – – – 0 0 0 0 0 2
Mitochondria 0 0 3 0 0 4 – 18 23 16 48 40 0 0 0 – – – 0 1 2 0 2 2
Nucleus 0 0 1 0 0 0 – 0 0 0 0 0 0 0 1 – – – 1 1 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 3 0 0 2 1 0 0 1 – – – 0 4 2 2 2 2
Unique Cell Count 259 323 230 211 182 233 246 199 187 190 223 210 194 310 241 310 232 185 177 188
Labelled Cell Count 262 328 236 213 184 242 274 218 211 231 256 211 202 316 241 310 232 185 177 188


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 50.2 57.8 31.2 50.3 51.1 41.8 – 34.0 30.5 30.6 27.0 26.2 54.2 54.5 53.6 – – – 53.1 58.5 57.0
Std Deviation (1e-4) 12.3 10.8 8.4 10.4 12.2 9.4 – 8.6 6.7 7.9 6.7 6.8 13.7 11.4 10.3 – – – 11.9 18.4 18.3
Intensity Change (Log2) – – – 0.69 0.71 0.42 – 0.12 -0.03 -0.03 -0.21 -0.26 0.8 0.8 0.78 – – – 0.76 0.91 0.87


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 – 0 0 3.3 2.9 2.2 0 0 1.8 – – –
Cytoplasm 1.4 -0.5 -2.9 – -7.4 -6.1 -8.7 -8.0 -8.5 -1.1 1.2 -1.1 – – –
Endoplasmic Reticulum 0 0.1 2.5 – 5.4 4.0 6.8 4.0 6.2 0.4 0 -0.1 – – –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 – 3.8 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 – 3.2 4.4 3.5 7.5 6.0 0 0 0 – – –
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 82.1796 121.3527 110.5823 94.0008 98.7552 93.2452 66.8249 103.9227 101.0622 89.6284 77.6343 97.9287 98.1046 123.3063 115.4588 114.3482 107.2465 114.4426
Actin 0.0023 0.0004 0.008 0.0007 0.0003 0.0025 0.0383 0.0008 0.0026 0.0117 0.0059 0.0022 0.0012 0.0002 0.0008 0.0001 0.0006 0.001
Bud 0.0033 0.0005 0.0028 0.0017 0.0011 0.0035 0.0048 0.0009 0.0015 0.0032 0.006 0.0005 0.0009 0.0003 0.0012 0.0006 0.0002 0.0007
Bud Neck 0.0006 0.0005 0.0008 0.0005 0.0008 0.0012 0.0048 0.0006 0.0005 0.0127 0.0008 0.0013 0.0006 0.0005 0.0007 0.0003 0.0004 0.0014
Bud Periphery 0.0038 0.0003 0.0025 0.0007 0.0006 0.004 0.0094 0.0004 0.001 0.006 0.0125 0.0001 0.0011 0.0002 0.0017 0.0001 0.0001 0.0004
Bud Site 0.0013 0.0007 0.0022 0.0005 0.0002 0.0006 0.0053 0.0006 0.0015 0.0047 0.0017 0.0001 0.0005 0.0002 0.0004 0.0001 0.0001 0.0001
Cell Periphery 0.01 0.0128 0.0164 0.0191 0.0016 0.0006 0.0085 0.0066 0.008 0.0086 0.0021 0.0014 0.0128 0.0118 0.0102 0.0049 0.0012 0.0087
Cytoplasm 0.702 0.9067 0.801 0.636 0.8126 0.7027 0.6432 0.9357 0.8655 0.7249 0.5535 0.9605 0.8136 0.928 0.8656 0.8099 0.8816 0.8438
Cytoplasmic Foci 0.0159 0.0023 0.0099 0.0407 0.0258 0.0337 0.0159 0.0015 0.005 0.0024 0.0055 0.0072 0.0017 0.0012 0.0025 0.0018 0.0067 0.0062
Eisosomes 0.0002 0.0005 0.001 0.0003 0.0002 0.0001 0.001 0 0.0001 0.0016 0.0003 0 0.0015 0 0 0 0 0.0065
Endoplasmic Reticulum 0.0541 0.019 0.0132 0.004 0.011 0.0079 0.0634 0.0076 0.0073 0.0031 0.0165 0.0036 0.0349 0.0096 0.0262 0.0016 0.0022 0.0465
Endosome 0.0192 0.002 0.0052 0.0114 0.0191 0.1005 0.0208 0.0023 0.0067 0.0601 0.0403 0.0097 0.0084 0.0024 0.0075 0.0053 0.01 0.0291
Golgi 0.003 0.0007 0.0019 0.0017 0.001 0.0217 0.0089 0.0006 0.0016 0.005 0.0045 0.002 0.0012 0.0004 0.0018 0.0001 0.0008 0.0073
Lipid Particles 0.0065 0.0012 0.0031 0.0464 0.0171 0.0035 0.0068 0.0006 0.0018 0.0011 0.0006 0.001 0.0013 0.0033 0.0014 0.0003 0.0059 0.0038
Mitochondria 0.014 0.0011 0.0106 0.023 0.0027 0.0205 0.0142 0.0004 0.0271 0.0067 0.1068 0.0005 0.0095 0.0001 0.0017 0.0001 0.0005 0.0031
None 0.0113 0.0005 0.0075 0.0051 0.0039 0.0054 0.0106 0.0002 0.0009 0.0004 0.0026 0.0005 0.0015 0.0001 0.0003 0.0001 0.0002 0.0007
Nuclear Periphery 0.021 0.0028 0.0072 0.0092 0.0067 0.0068 0.0236 0.0022 0.0031 0.0052 0.0161 0.0015 0.0146 0.0021 0.0053 0.004 0.0035 0.0091
Nucleolus 0.0108 0.0016 0.0033 0.007 0.0014 0.0038 0.007 0.0001 0.0011 0.0018 0.0003 0.0002 0.0018 0.0002 0.0002 0.0002 0.0001 0.0005
Nucleus 0.0113 0.002 0.0043 0.0058 0.0023 0.0058 0.0105 0.0014 0.002 0.0041 0.0034 0.0012 0.0071 0.0011 0.0018 0.0023 0.0012 0.0017
Peroxisomes 0.0018 0.0001 0.0005 0.0041 0.0006 0.001 0.0018 0 0.0008 0.0002 0.0005 0.0001 0.0001 0 0 0 0.0283 0.0001
Punctate Nuclear 0.0017 0 0.0006 0.0014 0.0003 0.0158 0.0027 0 0.0004 0.0001 0.0001 0.0001 0.0003 0 0 0 0.0002 0.0001
Vacuole 0.0842 0.04 0.0837 0.1316 0.0664 0.035 0.08 0.0345 0.0509 0.1118 0.1356 0.0046 0.0727 0.0349 0.0606 0.1377 0.0482 0.0187
Vacuole Periphery 0.0218 0.0041 0.0142 0.0492 0.0242 0.0233 0.0185 0.0031 0.0105 0.0245 0.0845 0.0017 0.0129 0.003 0.0099 0.0304 0.0081 0.0104

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1966.4085 2131.2063 2320.7295 2022.0366 2138.7364 1620.9737 1826.4245 1680.1128 1695.8021 1641.8428
Translational Efficiency 1.4444 1.4552 1.2884 1.3436 1.4834 1.6299 1.3207 1.3959 1.2756 1.4412

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘