Standard name
Description Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.05 0 0.06 0.08 0.06 0.09 0.11 0.12 0.16 0 0 0.06 0.05 0.05 0.10 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.06 0 0.13 0.11 0.06 0.08 0.06 0.05 0.08 0.09 0.12 0.12 0.05 0.18 0.14 0.61 0.62 0.53 0 0 0 0 0 0
Cytoplasm 0.16 0.19 0.10 0.14 0.18 0.16 0.13 0.15 0.21 0.15 0.21 0.22 0.16 0.18 0.08 0.32 0.34 0.29 0.05 0.08 0 0.07 0.07 0.09
Endoplasmic Reticulum 0.87 0.84 0.87 0.80 0.80 0.76 0.77 0.71 0.61 0.68 0.60 0.58 0.83 0.71 0.90 0 0 0 0.92 0.88 0.91 0.78 0.79 0.77
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.12 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.10 0 0.09 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 11 14 12 18 37 23 32 54 33 64 1 2 8 7 8 12 1 1 1 2 6 5
Bud 2 0 4 3 2 7 6 7 1 6 9 16 0 1 2 2 3 2 0 0 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 11 7 37 28 19 27 27 21 31 43 34 49 5 20 17 92 91 61 2 1 8 4 8 5
Cytoplasm 30 34 27 36 54 52 62 56 77 75 57 88 18 20 10 48 50 33 9 15 8 12 15 21
Endoplasmic Reticulum 166 149 238 210 240 246 364 275 223 329 164 236 92 78 112 4 2 2 175 173 260 142 183 188
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 2 0 4 17 4 0 6 1 6 17 7 7 1 1 1 9 6 14 0 1 1 2 4 4
Mitochondria 0 0 2 1 5 8 12 8 35 18 25 51 0 1 0 3 2 4 0 0 1 3 1 1
Nucleus 0 0 0 0 0 1 3 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 4 0 0 1 3 1 2 2 3 1 0 0 0 0 0 0 0 0 1 1 0 0
Nucleolus 0 0 0 0 0 0 0 4 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 5 3 3 8 29 15 35 10 8 3 3 1 1 2 0 0 1 1 5 4 8
Unique Cell Count 190 178 275 261 301 325 471 386 367 484 273 404 111 110 124 152 146 115 192 197 288 184 231 245
Labelled Cell Count 216 194 327 314 339 363 528 425 422 579 342 523 120 126 151 166 164 128 192 197 288 184 231 245


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 37.2 34.6 24.0 29.9 29.0 24.7 22.6 22.6 22.7 22.8 22.9 22.7 38.5 33.4 33.6 36.3 37.9 41.8 36.8 41.0 41.3
Std Deviation (1e-4) 6.7 6.3 4.4 6.4 6.9 5.6 5.4 5.4 5.8 6.0 5.9 6.3 9.3 8.9 7.5 9.1 9.5 8.7 9.8 10.6 12.1
Intensity Change (Log2) 0.32 0.27 0.04 -0.08 -0.09 -0.08 -0.07 -0.07 -0.08 0.68 0.48 0.48 0.6 0.66 0.8 0.62 0.77 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.7 0 0.9 2.1 1.1 2.4 3.4 3.5 4.8 0 0 1.1 0.3 0.7 2.5
Bud 0 0 0.6 0 0 0 0 1.4 1.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.0 -2.9 -2.0 -3.6 -3.6 -2.0 -2.0 -0.3 -0.5 -2.6 1.2 0.1 10.1 10.4 8.2
Cytoplasm 1.4 2.8 2.2 1.4 1.8 3.8 2.2 3.6 4.1 1.8 2.3 -0.6 5.7 6.2 4.7
Endoplasmic Reticulum -1.9 -2.2 -3.4 -3.1 -4.7 -7.2 -5.7 -7.0 -7.8 -0.9 -3.6 1.1 -16.8 -16.8 -15.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.0 0 0 0 0 0 1.7 0.9 0 0 0 0 0 0 4.6
Mitochondria 0 0 0 1.8 0 4.7 2.5 4.6 5.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.6 3.4 4.6 0 0 0 0 0 0 0 0