Standard name | |
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Description | Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 4 | 11 | 14 | 12 | 18 | 37 | 23 | 32 | 54 | 33 | 64 | 1 | 2 | 8 | 7 | 8 | 12 | 1 | 1 | 1 | 2 | 6 | 5 |
Bud | 2 | 0 | 4 | 3 | 2 | 7 | 6 | 7 | 1 | 6 | 9 | 16 | 0 | 1 | 2 | 2 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 11 | 7 | 37 | 28 | 19 | 27 | 27 | 21 | 31 | 43 | 34 | 49 | 5 | 20 | 17 | 92 | 91 | 61 | 2 | 1 | 8 | 4 | 8 | 5 |
Cytoplasm | 30 | 34 | 27 | 36 | 54 | 52 | 62 | 56 | 77 | 75 | 57 | 88 | 18 | 20 | 10 | 48 | 50 | 33 | 9 | 15 | 8 | 12 | 15 | 21 |
Endoplasmic Reticulum | 166 | 149 | 238 | 210 | 240 | 246 | 364 | 275 | 223 | 329 | 164 | 236 | 92 | 78 | 112 | 4 | 2 | 2 | 175 | 173 | 260 | 142 | 183 | 188 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Golgi | 2 | 0 | 4 | 17 | 4 | 0 | 6 | 1 | 6 | 17 | 7 | 7 | 1 | 1 | 1 | 9 | 6 | 14 | 0 | 1 | 1 | 2 | 4 | 4 |
Mitochondria | 0 | 0 | 2 | 1 | 5 | 8 | 12 | 8 | 35 | 18 | 25 | 51 | 0 | 1 | 0 | 3 | 2 | 4 | 0 | 0 | 1 | 3 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 4 | 0 | 0 | 1 | 3 | 1 | 2 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 5 | 3 | 3 | 8 | 29 | 15 | 35 | 10 | 8 | 3 | 3 | 1 | 1 | 2 | 0 | 0 | 1 | 1 | 5 | 4 | 8 |
Unique Cell Count | 190 | 178 | 275 | 261 | 301 | 325 | 471 | 386 | 367 | 484 | 273 | 404 | 111 | 110 | 124 | 152 | 146 | 115 | 192 | 197 | 288 | 184 | 231 | 245 |
Labelled Cell Count | 216 | 194 | 327 | 314 | 339 | 363 | 528 | 425 | 422 | 579 | 342 | 523 | 120 | 126 | 151 | 166 | 164 | 128 | 192 | 197 | 288 | 184 | 231 | 245 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 37.2 | 34.6 | 24.0 | 29.9 | 29.0 | 24.7 | 22.6 | 22.6 | 22.7 | 22.8 | 22.9 | 22.7 | 38.5 | 33.4 | 33.6 | 36.3 | 37.9 | 41.8 | 36.8 | 41.0 | 41.3 |
Std Deviation (1e-4) | 6.7 | 6.3 | 4.4 | 6.4 | 6.9 | 5.6 | 5.4 | 5.4 | 5.8 | 6.0 | 5.9 | 6.3 | 9.3 | 8.9 | 7.5 | 9.1 | 9.5 | 8.7 | 9.8 | 10.6 | 12.1 |
Intensity Change (Log2) | – | – | – | 0.32 | 0.27 | 0.04 | -0.08 | -0.09 | -0.08 | -0.07 | -0.07 | -0.08 | 0.68 | 0.48 | 0.48 | 0.6 | 0.66 | 0.8 | 0.62 | 0.77 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.7 | 0 | 0.9 | 2.1 | 1.1 | 2.4 | 3.4 | 3.5 | 4.8 | 0 | 0 | 1.1 | 0.3 | 0.7 | 2.5 |
Bud | 0 | 0 | 0.6 | 0 | 0 | 0 | 0 | 1.4 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.0 | -2.9 | -2.0 | -3.6 | -3.6 | -2.0 | -2.0 | -0.3 | -0.5 | -2.6 | 1.2 | 0.1 | 10.1 | 10.4 | 8.2 |
Cytoplasm | 1.4 | 2.8 | 2.2 | 1.4 | 1.8 | 3.8 | 2.2 | 3.6 | 4.1 | 1.8 | 2.3 | -0.6 | 5.7 | 6.2 | 4.7 |
Endoplasmic Reticulum | -1.9 | -2.2 | -3.4 | -3.1 | -4.7 | -7.2 | -5.7 | -7.0 | -7.8 | -0.9 | -3.6 | 1.1 | -16.8 | -16.8 | -15.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3.0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 |
Mitochondria | 0 | 0 | 0 | 1.8 | 0 | 4.7 | 2.5 | 4.6 | 5.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 4.6 | 3.4 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 80.0962 | 77.5305 | 81.7096 | 77.6399 | 73.9651 | 81.4317 | 83.1994 | 82.6829 | 87.8545 | 86.6779 | 81.5557 | 84.0268 | 87.0265 | 106.105 | 100.9513 | 81.8845 | 105.9571 | 102.6312 |
Actin | 0.0051 | 0.0006 | 0.0032 | 0.0001 | 0.001 | 0.0002 | 0.0168 | 0.0009 | 0.0013 | 0.0001 | 0.0028 | 0.0007 | 0.0311 | 0.0011 | 0.0198 | 0.0015 | 0.0083 | 0.0075 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0004 |
Bud Neck | 0.0005 | 0.0005 | 0.0011 | 0.0008 | 0.002 | 0.0014 | 0.001 | 0.0001 | 0.0006 | 0.0003 | 0.0027 | 0.0014 | 0.0005 | 0.0006 | 0.0009 | 0.0007 | 0.0086 | 0.0056 |
Bud Periphery | 0.001 | 0.0002 | 0.0005 | 0 | 0.0012 | 0.0002 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.001 | 0.0007 | 0.0004 | 0.0005 | 0.0006 | 0.0005 | 0.0021 |
Bud Site | 0.0008 | 0.0005 | 0.0003 | 0 | 0.0007 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0005 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0002 |
Cell Periphery | 0.0328 | 0.0399 | 0.0553 | 0.0273 | 0.0404 | 0.0162 | 0.0323 | 0.019 | 0.0382 | 0.0487 | 0.0128 | 0.0119 | 0.025 | 0.0912 | 0.0827 | 0.0612 | 0.0813 | 0.0611 |
Cytoplasm | 0.0334 | 0.0227 | 0.0161 | 0.0104 | 0.0646 | 0.0144 | 0.013 | 0.0095 | 0.0107 | 0.0016 | 0.0212 | 0.0045 | 0.0832 | 0.0317 | 0.0437 | 0.0017 | 0.0444 | 0.0038 |
Cytoplasmic Foci | 0.0039 | 0.0002 | 0.0038 | 0.0002 | 0.0038 | 0.0002 | 0.0043 | 0.0004 | 0.0002 | 0.0001 | 0.0047 | 0.0003 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0003 |
Eisosomes | 0.0009 | 0.0002 | 0.0008 | 0.0001 | 0.0011 | 0.0002 | 0.0015 | 0.0058 | 0.0005 | 0.0007 | 0.0014 | 0.0006 | 0.0015 | 0.0109 | 0.0096 | 0.0008 | 0.0004 | 0.0068 |
Endoplasmic Reticulum | 0.7979 | 0.8995 | 0.837 | 0.9342 | 0.78 | 0.932 | 0.7863 | 0.9237 | 0.9028 | 0.8918 | 0.8629 | 0.9212 | 0.5618 | 0.7673 | 0.717 | 0.7861 | 0.7932 | 0.7058 |
Endosome | 0.0017 | 0.0003 | 0.0018 | 0.0003 | 0.0058 | 0.0004 | 0.0031 | 0.0006 | 0.0004 | 0.0008 | 0.0035 | 0.0013 | 0.0096 | 0.0004 | 0.003 | 0.0037 | 0.0007 | 0.0047 |
Golgi | 0.0015 | 0.0006 | 0.002 | 0.0011 | 0.0009 | 0.001 | 0.0042 | 0.0015 | 0.0013 | 0.0058 | 0.004 | 0.0041 | 0.0132 | 0.0013 | 0.0037 | 0.0026 | 0.003 | 0.0086 |
Lipid Particles | 0.0071 | 0.0133 | 0.0096 | 0.0075 | 0.01 | 0.0044 | 0.0065 | 0.0034 | 0.0059 | 0.0021 | 0.011 | 0.0035 | 0.0021 | 0.015 | 0.0036 | 0.0017 | 0.0067 | 0.0144 |
Mitochondria | 0.0039 | 0.0001 | 0.0035 | 0 | 0.0109 | 0.0014 | 0.0007 | 0.0011 | 0.0001 | 0 | 0.008 | 0.0001 | 0.0092 | 0.0009 | 0.0017 | 0.0006 | 0 | 0.0027 |
None | 0.0024 | 0.0001 | 0.0045 | 0.0001 | 0.0126 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0135 | 0.0002 | 0.0012 | 0.0003 | 0.0008 | 0.0003 | 0.0001 | 0.0009 |
Nuclear Periphery | 0.0782 | 0.0133 | 0.0415 | 0.0146 | 0.0233 | 0.0185 | 0.1088 | 0.0293 | 0.0303 | 0.0415 | 0.0332 | 0.043 | 0.2275 | 0.0625 | 0.095 | 0.1181 | 0.0368 | 0.1416 |
Nucleolus | 0.0041 | 0.0001 | 0.0027 | 0 | 0.0174 | 0.0008 | 0.0001 | 0 | 0.0001 | 0 | 0.0016 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0014 |
Nucleus | 0.0038 | 0.0003 | 0.0016 | 0.0002 | 0.0046 | 0.0023 | 0.0044 | 0.0008 | 0.0003 | 0.0002 | 0.0019 | 0.0007 | 0.014 | 0.0011 | 0.0041 | 0.0019 | 0.0007 | 0.0038 |
Peroxisomes | 0.0008 | 0 | 0.0003 | 0 | 0.001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0025 | 0 | 0.0025 | 0 | 0.0024 | 0 | 0.0007 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0004 |
Vacuole | 0.014 | 0.0073 | 0.0092 | 0.003 | 0.012 | 0.0042 | 0.0089 | 0.0026 | 0.006 | 0.0053 | 0.0076 | 0.0034 | 0.0074 | 0.0129 | 0.0098 | 0.0124 | 0.0134 | 0.0137 |
Vacuole Periphery | 0.0034 | 0.0002 | 0.0026 | 0.0002 | 0.0041 | 0.002 | 0.0047 | 0.0008 | 0.0009 | 0.0008 | 0.0056 | 0.0018 | 0.0096 | 0.0011 | 0.002 | 0.0058 | 0.0009 | 0.014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 143.4269 | 116.6228 | 190.8032 | 226.7519 | 166.5991 | 134.3146 | 101.5793 | 115.0643 | 152.2839 | 201.3494 |
Translational Efficiency | 1.0512 | 0.9031 | 1.1663 | 0.8745 | 0.9946 | 1.2848 | 0.9981 | 0.9836 | 1.0203 | 0.9823 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |