Standard name
Description Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.05 0 0.06 0.08 0.06 0.09 0.11 0.12 0.16 0 0 0.06 0.05 0.05 0.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0 0.13 0.11 0.06 0.08 0.06 0.05 0.08 0.09 0.12 0.12 0.05 0.18 0.14 0.61 0.62 0.53 0 0 0 0 0 0
Cytoplasm 0.16 0.19 0.1 0.14 0.18 0.16 0.13 0.15 0.21 0.15 0.21 0.22 0.16 0.18 0.08 0.32 0.34 0.29 0.05 0.08 0 0.07 0.07 0.09
Endoplasmic Reticulum 0.87 0.84 0.87 0.8 0.8 0.76 0.77 0.71 0.61 0.68 0.6 0.58 0.83 0.71 0.9 0 0 0 0.92 0.88 0.91 0.78 0.79 0.77
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.12 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.1 0 0.09 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 11 14 12 18 37 23 32 54 33 64 1 2 8 7 8 12 1 1 1 2 6 5
Bud 2 0 4 3 2 7 6 7 1 6 9 16 0 1 2 2 3 2 0 0 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 11 7 37 28 19 27 27 21 31 43 34 49 5 20 17 92 91 61 2 1 8 4 8 5
Cytoplasm 30 34 27 36 54 52 62 56 77 75 57 88 18 20 10 48 50 33 9 15 8 12 15 21
Endoplasmic Reticulum 166 149 238 210 240 246 364 275 223 329 164 236 92 78 112 4 2 2 175 173 260 142 183 188
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 2 0 4 17 4 0 6 1 6 17 7 7 1 1 1 9 6 14 0 1 1 2 4 4
Mitochondria 0 0 2 1 5 8 12 8 35 18 25 51 0 1 0 3 2 4 0 0 1 3 1 1
Nucleus 0 0 0 0 0 1 3 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 4 0 0 1 3 1 2 2 3 1 0 0 0 0 0 0 0 0 1 1 0 0
Nucleolus 0 0 0 0 0 0 0 4 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 5 3 3 8 29 15 35 10 8 3 3 1 1 2 0 0 1 1 5 4 8
Unique Cell Count 190 178 275 261 301 325 471 386 367 484 273 404 111 110 124 152 146 115 192 197 288 184 231 245
Labelled Cell Count 216 194 327 314 339 363 528 425 422 579 342 523 120 126 151 166 164 128 192 197 288 184 231 245


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 37.2 34.6 24.0 29.9 29.0 24.7 22.6 22.6 22.7 22.8 22.9 22.7 38.5 33.4 33.6 36.3 37.9 41.8 36.8 41.0 41.3
Std Deviation (1e-4) 6.7 6.3 4.4 6.4 6.9 5.6 5.4 5.4 5.8 6.0 5.9 6.3 9.3 8.9 7.5 9.1 9.5 8.7 9.8 10.6 12.1
Intensity Change (Log2) – – – 0.32 0.27 0.04 -0.08 -0.09 -0.08 -0.07 -0.07 -0.08 0.68 0.48 0.48 0.6 0.66 0.8 0.62 0.77 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.7 0 0.9 2.1 1.1 2.4 3.4 3.5 4.8 0 0 1.1 0.3 0.7 2.5
Bud 0 0 0.6 0 0 0 0 1.4 1.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.0 -2.9 -2.0 -3.6 -3.6 -2.0 -2.0 -0.3 -0.5 -2.6 1.2 0.1 10.1 10.4 8.2
Cytoplasm 1.4 2.8 2.2 1.4 1.8 3.8 2.2 3.6 4.1 1.8 2.3 -0.6 5.7 6.2 4.7
Endoplasmic Reticulum -1.9 -2.2 -3.4 -3.1 -4.7 -7.2 -5.7 -7.0 -7.8 -0.9 -3.6 1.1 -16.8 -16.8 -15.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.0 0 0 0 0 0 1.7 0.9 0 0 0 0 0 0 4.6
Mitochondria 0 0 0 1.8 0 4.7 2.5 4.6 5.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.6 3.4 4.6 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 80.0962 77.5305 81.7096 77.6399 73.9651 81.4317 83.1994 82.6829 87.8545 86.6779 81.5557 84.0268 87.0265 106.105 100.9513 81.8845 105.9571 102.6312
Actin 0.0051 0.0006 0.0032 0.0001 0.001 0.0002 0.0168 0.0009 0.0013 0.0001 0.0028 0.0007 0.0311 0.0011 0.0198 0.0015 0.0083 0.0075
Bud 0.0002 0.0001 0.0001 0 0.0003 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0005 0.0001 0.0003 0.0001 0.0001 0.0004
Bud Neck 0.0005 0.0005 0.0011 0.0008 0.002 0.0014 0.001 0.0001 0.0006 0.0003 0.0027 0.0014 0.0005 0.0006 0.0009 0.0007 0.0086 0.0056
Bud Periphery 0.001 0.0002 0.0005 0 0.0012 0.0002 0.0005 0 0.0002 0.0001 0.0003 0.001 0.0007 0.0004 0.0005 0.0006 0.0005 0.0021
Bud Site 0.0008 0.0005 0.0003 0 0.0007 0 0.0002 0 0.0001 0 0.0003 0 0.0005 0.0003 0.0006 0.0001 0.0001 0.0002
Cell Periphery 0.0328 0.0399 0.0553 0.0273 0.0404 0.0162 0.0323 0.019 0.0382 0.0487 0.0128 0.0119 0.025 0.0912 0.0827 0.0612 0.0813 0.0611
Cytoplasm 0.0334 0.0227 0.0161 0.0104 0.0646 0.0144 0.013 0.0095 0.0107 0.0016 0.0212 0.0045 0.0832 0.0317 0.0437 0.0017 0.0444 0.0038
Cytoplasmic Foci 0.0039 0.0002 0.0038 0.0002 0.0038 0.0002 0.0043 0.0004 0.0002 0.0001 0.0047 0.0003 0.0006 0.0002 0.0004 0.0001 0.0006 0.0003
Eisosomes 0.0009 0.0002 0.0008 0.0001 0.0011 0.0002 0.0015 0.0058 0.0005 0.0007 0.0014 0.0006 0.0015 0.0109 0.0096 0.0008 0.0004 0.0068
Endoplasmic Reticulum 0.7979 0.8995 0.837 0.9342 0.78 0.932 0.7863 0.9237 0.9028 0.8918 0.8629 0.9212 0.5618 0.7673 0.717 0.7861 0.7932 0.7058
Endosome 0.0017 0.0003 0.0018 0.0003 0.0058 0.0004 0.0031 0.0006 0.0004 0.0008 0.0035 0.0013 0.0096 0.0004 0.003 0.0037 0.0007 0.0047
Golgi 0.0015 0.0006 0.002 0.0011 0.0009 0.001 0.0042 0.0015 0.0013 0.0058 0.004 0.0041 0.0132 0.0013 0.0037 0.0026 0.003 0.0086
Lipid Particles 0.0071 0.0133 0.0096 0.0075 0.01 0.0044 0.0065 0.0034 0.0059 0.0021 0.011 0.0035 0.0021 0.015 0.0036 0.0017 0.0067 0.0144
Mitochondria 0.0039 0.0001 0.0035 0 0.0109 0.0014 0.0007 0.0011 0.0001 0 0.008 0.0001 0.0092 0.0009 0.0017 0.0006 0 0.0027
None 0.0024 0.0001 0.0045 0.0001 0.0126 0.0001 0.0013 0.0001 0.0001 0.0001 0.0135 0.0002 0.0012 0.0003 0.0008 0.0003 0.0001 0.0009
Nuclear Periphery 0.0782 0.0133 0.0415 0.0146 0.0233 0.0185 0.1088 0.0293 0.0303 0.0415 0.0332 0.043 0.2275 0.0625 0.095 0.1181 0.0368 0.1416
Nucleolus 0.0041 0.0001 0.0027 0 0.0174 0.0008 0.0001 0 0.0001 0 0.0016 0 0.0002 0.0003 0.0002 0.0001 0.0001 0.0014
Nucleus 0.0038 0.0003 0.0016 0.0002 0.0046 0.0023 0.0044 0.0008 0.0003 0.0002 0.0019 0.0007 0.014 0.0011 0.0041 0.0019 0.0007 0.0038
Peroxisomes 0.0008 0 0.0003 0 0.001 0 0.0004 0 0 0 0.0003 0 0.0001 0 0.0001 0 0 0.0001
Punctate Nuclear 0.0025 0 0.0025 0 0.0024 0 0.0007 0.0001 0 0 0.0004 0.0001 0.0007 0.0001 0.0005 0.0001 0.0002 0.0004
Vacuole 0.014 0.0073 0.0092 0.003 0.012 0.0042 0.0089 0.0026 0.006 0.0053 0.0076 0.0034 0.0074 0.0129 0.0098 0.0124 0.0134 0.0137
Vacuole Periphery 0.0034 0.0002 0.0026 0.0002 0.0041 0.002 0.0047 0.0008 0.0009 0.0008 0.0056 0.0018 0.0096 0.0011 0.002 0.0058 0.0009 0.014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 143.4269 116.6228 190.8032 226.7519 166.5991 134.3146 101.5793 115.0643 152.2839 201.3494
Translational Efficiency 1.0512 0.9031 1.1663 0.8745 0.9946 1.2848 0.9981 0.9836 1.0203 0.9823

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘