Standard name
Description Vacuolar membrane protein; vacuole-specific Myo2p receptor and Myo2p-Vac17p-Vac8p transport complex subunit required for vacuolar inheritance; required with Atg13p for the vesicle closure step of the cytoplasm-to-vacuole (CVT) pathway, for homotypic vacuole-vacuole fusion and for nucleus-vacuole junction formation with Nvy1p; contains 11 armadillo (ARM) repeats; myristoylated, palmitoylated, and phosphorylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0.05 – 0.07 0.11 0.07 0 0 0 0.07 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.06 0 0.08 0.18 0.12 0.05 0 0.08 – 0.05 0 0.07 0.09 0.05 0.05 0.08 0.12 0.07 0.1 0.09 0.24 0.06 0.06 0.07
Golgi 0.05 0 0 0 0.17 0.13 0.22 0.15 – 0.15 0.07 0.15 0.05 0.06 0.07 0.06 0.08 0.12 0 0 0 0 0 0
Mitochondria 0.11 0.17 0.3 0.37 0.13 0.26 0.25 0.32 – 0.37 0.35 0.28 0 0 0 0.05 0.08 0.05 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0.07 0.13 0.11 0 0 0.11 0 0 0
Nucleolus 0.3 0.26 0.44 0.41 0.22 0.18 0.12 0.16 – 0.14 0.13 0.09 0.35 0.25 0.28 0.22 0.25 0.28 0.05 0.05 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.73 0.79 0.6 0.47 0.69 0.62 0.57 0.43 – 0.47 0.49 0.56 0.7 0.81 0.82 0.72 0.69 0.77 0.76 0.77 0.52 0.78 0.79 0.76
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 1 7 – 3 6 3 1 0 0 1 0 0 0 0 1 0 0 0
Bud 0 0 2 0 1 1 1 4 – 1 0 4 0 0 0 0 0 0 0 1 1 0 1 6
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 1 4 – 2 4 2 0 0 2 0 0 1 0 0 0 0 0 0
Cytoplasm 3 0 12 8 15 10 19 29 – 31 39 29 9 13 17 19 12 11 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 2 0 0 7 12 16 – 25 10 17 1 4 0 11 1 11 0 0 0 0 1 1
Endosome 14 1 30 49 33 18 19 44 – 19 11 30 76 45 49 23 19 13 25 10 92 16 22 27
Golgi 12 4 14 11 47 49 93 86 – 63 24 66 44 53 71 17 12 24 1 1 3 0 3 1
Mitochondria 26 15 109 103 36 95 105 180 – 157 123 123 27 11 10 14 12 9 6 1 24 6 4 4
Nucleus 9 4 1 1 3 8 4 4 – 2 4 1 9 20 23 14 11 2 3 4 1 9 10 16
Nuclear Periphery 11 4 13 11 7 17 19 22 – 8 8 7 30 16 12 20 20 21 10 1 42 8 15 9
Nucleolus 74 23 159 113 59 64 51 87 – 58 47 40 289 219 296 62 39 56 13 5 8 9 13 23
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 0 0 – 0 0 0 11 3 4 5 4 2 1 0 3 2 0 0
Vac/Vac Membrane 179 71 218 128 189 227 244 240 – 196 171 243 581 707 868 208 107 153 199 90 201 206 285 294
Unique Cell Count 244 90 365 275 272 364 428 558 419 350 435 830 876 1063 287 154 199 263 117 385 263 361 388
Labelled Cell Count 330 122 560 425 391 496 569 723 565 447 565 1078 1091 1352 394 237 303 263 117 385 263 361 388


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.9 24.6 22.3 20.2 20.7 18.1 17.6 17.3 – 16.4 16.0 17.5 21.4 24.0 25.9 14.9 15.2 21.7 24.2 26.4 27.8
Std Deviation (1e-4) 5.5 7.2 6.0 5.2 5.7 5.0 4.6 4.6 – 3.9 3.8 4.3 6.0 6.4 6.4 5.3 6.7 8.2 7.1 8.4 8.6
Intensity Change (Log2) – – – -0.14 -0.11 -0.3 -0.34 -0.37 – -0.44 -0.47 -0.35 -0.06 0.11 0.22 -0.58 -0.55 -0.04 0.12 0.24 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 1.4 -0.4 0.8 1.4 – 2.5 4.1 2.2 -2.7 -2.1 -2.0 2.0 2.2 1.3
Endoplasmic Reticulum 0 0 0 2.4 2.5 – 4.2 2.4 3.1 0 0 0 3.0 0 0
Endosome 3.7 1.6 -1.8 -2.2 -0.2 – -2.1 -2.9 -0.7 0.5 -2.1 -2.6 -0.1 1.5 -0.7
Golgi 0.1 5.7 4.6 7.4 5.5 – 5.3 1.8 5.3 1.1 1.6 2.0 1.2 1.9 3.7
Mitochondria 2.0 -5.0 -1.1 -1.7 0.8 – 2.2 1.5 -0.5 -13.3 -15.5 -17.2 -8.1 -5.4 -7.1
Nucleus 0 0 0 0 0 – 0 0 0 0 2.5 2.4 3.9 0 0
Nuclear Periphery 0.3 -0.7 0.8 0.6 0.3 – -1.4 -1.0 -1.8 0 -1.8 -3.1 2.0 4.0 3.3
Nucleolus -0.6 -5.8 -7.6 -10.1 -9.4 – -9.3 -8.9 -11.2 -2.9 -6.5 -5.6 -5.9 -3.9 -3.6
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -3.3 2.5 0.7 -0.8 -5.0 – -3.6 -2.9 -1.1 3.5 7.7 8.5 3.4 2.1 4.1