Standard name
Human Ortholog
Description Vacuolar membrane protein; vacuole-specific Myo2p receptor and Myo2p-Vac17p-Vac8p transport complex subunit required for vacuolar inheritance; required with Atg13p for the vesicle closure step of the cytoplasm-to-vacuole (CVT) pathway, for homotypic vacuole-vacuole fusion and for nucleus-vacuole junction formation with Nvy1p; contains 11 armadillo (ARM) repeats; myristoylated, palmitoylated, and phosphorylated

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0.05 0.07 0.11 0.07 0 0 0 0.07 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.06 0 0.08 0.18 0.12 0.05 0 0.08 0.05 0 0.07 0.09 0.05 0.05 0.08 0.12 0.07 0.1 0.09 0.24 0.06 0.06 0.07
Golgi 0.05 0 0 0 0.17 0.13 0.22 0.15 0.15 0.07 0.15 0.05 0.06 0.07 0.06 0.08 0.12 0 0 0 0 0 0
Mitochondria 0.11 0.17 0.3 0.37 0.13 0.26 0.25 0.32 0.37 0.35 0.28 0 0 0 0.05 0.08 0.05 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.07 0.13 0.11 0 0 0.11 0 0 0
Nucleolus 0.3 0.26 0.44 0.41 0.22 0.18 0.12 0.16 0.14 0.13 0.09 0.35 0.25 0.28 0.22 0.25 0.28 0.05 0.05 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.73 0.79 0.6 0.47 0.69 0.62 0.57 0.43 0.47 0.49 0.56 0.7 0.81 0.82 0.72 0.69 0.77 0.76 0.77 0.52 0.78 0.79 0.76
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 1 7 3 6 3 1 0 0 1 0 0 0 0 1 0 0 0
Bud 0 0 2 0 1 1 1 4 1 0 4 0 0 0 0 0 0 0 1 1 0 1 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 0 0 0 0 1 4 2 4 2 0 0 2 0 0 1 0 0 0 0 0 0
Cytoplasm 3 0 12 8 15 10 19 29 31 39 29 9 13 17 19 12 11 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 2 0 0 7 12 16 25 10 17 1 4 0 11 1 11 0 0 0 0 1 1
Endosome 14 1 30 49 33 18 19 44 19 11 30 76 45 49 23 19 13 25 10 92 16 22 27
Golgi 12 4 14 11 47 49 93 86 63 24 66 44 53 71 17 12 24 1 1 3 0 3 1
Mitochondria 26 15 109 103 36 95 105 180 157 123 123 27 11 10 14 12 9 6 1 24 6 4 4
Nucleus 9 4 1 1 3 8 4 4 2 4 1 9 20 23 14 11 2 3 4 1 9 10 16
Nuclear Periphery 11 4 13 11 7 17 19 22 8 8 7 30 16 12 20 20 21 10 1 42 8 15 9
Nucleolus 74 23 159 113 59 64 51 87 58 47 40 289 219 296 62 39 56 13 5 8 9 13 23
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 0 0 0 0 0 11 3 4 5 4 2 1 0 3 2 0 0
Vac/Vac Membrane 179 71 218 128 189 227 244 240 196 171 243 581 707 868 208 107 153 199 90 201 206 285 294
Unique Cell Count 244 90 365 275 272 364 428 558 419 350 435 830 876 1063 287 154 199 263 117 385 263 361 388
Labelled Cell Count 330 122 560 425 391 496 569 723 565 447 565 1078 1091 1352 394 237 303 263 117 385 263 361 388


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.9 24.6 22.3 20.2 20.7 18.1 17.6 17.3 16.4 16.0 17.5 21.4 24.0 25.9 14.9 15.2 21.7 24.2 26.4 27.8
Std Deviation (1e-4) 5.5 7.2 6.0 5.2 5.7 5.0 4.6 4.6 3.9 3.8 4.3 6.0 6.4 6.4 5.3 6.7 8.2 7.1 8.4 8.6
Intensity Change (Log2) -0.14 -0.11 -0.3 -0.34 -0.37 -0.44 -0.47 -0.35 -0.06 0.11 0.22 -0.58 -0.55 -0.04 0.12 0.24 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 1.4 -0.4 0.8 1.4 2.5 4.1 2.2 -2.7 -2.1 -2.0 2.0 2.2 1.3
Endoplasmic Reticulum 0 0 0 2.4 2.5 4.2 2.4 3.1 0 0 0 3.0 0 0
Endosome 3.7 1.6 -1.8 -2.2 -0.2 -2.1 -2.9 -0.7 0.5 -2.1 -2.6 -0.1 1.5 -0.7
Golgi 0.1 5.7 4.6 7.4 5.5 5.3 1.8 5.3 1.1 1.6 2.0 1.2 1.9 3.7
Mitochondria 2.0 -5.0 -1.1 -1.7 0.8 2.2 1.5 -0.5 -13.3 -15.5 -17.2 -8.1 -5.4 -7.1
Nucleus 0 0 0 0 0 0 0 0 0 2.5 2.4 3.9 0 0
Nuclear Periphery 0.3 -0.7 0.8 0.6 0.3 -1.4 -1.0 -1.8 0 -1.8 -3.1 2.0 4.0 3.3
Nucleolus -0.6 -5.8 -7.6 -10.1 -9.4 -9.3 -8.9 -11.2 -2.9 -6.5 -5.6 -5.9 -3.9 -3.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.3 2.5 0.7 -0.8 -5.0 -3.6 -2.9 -1.1 3.5 7.7 8.5 3.4 2.1 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.5519 20.4514 18.5575 16.7711 17.2692 18.5163 18.5766 21.0952 18.9966 20.2168 19.9982 19.4878 18.4812 23.1801 21.4737 19.7743 19.2333 21.0324
Actin 0.0026 0 0.0165 0.0015 0.0212 0 0.0154 0 0.0044 0 0 0.0083 0.0068 0 0.0012 0.0001 0 0.0004
Bud 0.0003 0 0.0002 0.0003 0.0001 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0 0 0 0
Bud Neck 0.0002 0 0.0009 0.0002 0.0001 0 0.0002 0.0001 0.0017 0 0 0.0003 0.0019 0 0.0001 0 0 0
Bud Periphery 0.0005 0.0001 0.0003 0.0006 0.0002 0.0002 0.0001 0.0001 0.0002 0 0 0.0002 0.0003 0.0001 0.0001 0 0.0001 0.0001
Bud Site 0.0007 0 0.008 0.0015 0.0001 0 0.0001 0.0001 0.0002 0 0 0.0003 0.0021 0 0.0001 0 0 0
Cell Periphery 0.0032 0.0031 0.0016 0.0013 0.0008 0.0013 0.0016 0.0025 0.0019 0.0007 0.0003 0.0012 0.0021 0.0011 0.0014 0.0023 0.001 0.0017
Cytoplasm 0.0096 0.0002 0.0042 0.0005 0.0006 0.0001 0.0004 0.0001 0.0007 0 0 0.0002 0.0016 0 0.0004 0 0 0.0005
Cytoplasmic Foci 0.0074 0.0006 0.0106 0.0018 0.0008 0.0137 0.0063 0.0017 0.0104 0.0008 0.0005 0.0065 0.0062 0.0009 0.0083 0.0002 0.0002 0.0017
Eisosomes 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0002 0 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0028 0.0001 0.0006 0.0007 0.0023 0.0001 0.008 0.0001 0.0009 0 0.0001 0.0003 0.0022 0.0001 0.0001 0 0.0001 0.0001
Endosome 0.0195 0.0042 0.0147 0.0063 0.0189 0.0035 0.0177 0.0066 0.0121 0.006 0.0059 0.0087 0.0188 0.0058 0.0077 0.0028 0.0057 0.0054
Golgi 0.003 0.0003 0.0057 0.001 0.0028 0.0005 0.0128 0.0014 0.0094 0.001 0.0013 0.0064 0.0041 0.0012 0.0025 0.0004 0.0006 0.0015
Lipid Particles 0.0429 0.018 0.0283 0.0144 0.006 0.0288 0.23 0.0819 0.0774 0.0704 0.0561 0.1554 0.0923 0.0563 0.0679 0.0337 0.0253 0.1094
Mitochondria 0.0151 0.0014 0.009 0.0071 0.0015 0.0034 0.0241 0.0089 0.0467 0.0016 0.0023 0.0157 0.0314 0.0022 0.031 0.0616 0.0014 0.0039
None 0.0025 0.0001 0.0009 0.0006 0.0008 0 0.0003 0 0.0041 0 0 0.0002 0.0049 0 0.003 0 0 0.0006
Nuclear Periphery 0.0409 0.0108 0.0131 0.0063 0.0209 0.0067 0.0083 0.0219 0.0047 0.0014 0.004 0.0137 0.0134 0.0333 0.0102 0.0006 0.0139 0.0149
Nucleolus 0.0221 0.0261 0.0224 0.0109 0.0021 0.0256 0.019 0.0248 0.0155 0.0033 0.0013 0.0088 0.0435 0.0072 0.0147 0.0081 0.0021 0.0309
Nucleus 0.0323 0.0059 0.0093 0.0112 0.0018 0.0112 0.0028 0.0024 0.0039 0.0005 0.0004 0.0016 0.0064 0.0015 0.0036 0.001 0.001 0.0042
Peroxisomes 0.0018 0.0001 0.0051 0.0005 0.0003 0.0001 0.0014 0.0003 0.0024 0.0001 0.0001 0.0036 0.0014 0.0001 0.0012 0.0001 0.0001 0.0003
Punctate Nuclear 0.0031 0 0.0031 0.0047 0.0021 0 0.0001 0.0001 0.0055 0 0 0.0006 0.0033 0.0001 0.0048 0 0 0.0034
Vacuole 0.2064 0.2596 0.2076 0.2576 0.1854 0.1272 0.0858 0.1508 0.1405 0.1564 0.0789 0.0939 0.1316 0.1296 0.1194 0.1708 0.1565 0.0852
Vacuole Periphery 0.583 0.6694 0.6376 0.671 0.731 0.7775 0.5653 0.6963 0.6572 0.7576 0.8488 0.6741 0.6255 0.7606 0.7222 0.7184 0.7921 0.7355

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 62.5707 48.2683 66.1564 83.18 68.3597 60.314 71.34 75.9544 82.5317 79.8039
Translational Efficiency 1.0308 1.3152 1.0247 1.0182 0.9361 1.0597 1.0921 0.9308 0.9892 0.8809

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
87 164 303 1588 164 1371 2745 1573 251 1535 3048 3161

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2359.83 1846.04 2914.74 2299.48 1626.86 1689.78 2369.66 2198.01 1880.92 1706.47 2423.85 2248.99
Standard Deviation 632.80 453.51 493.48 584.86 339.45 345.39 495.20 619.77 579.44 361.73 521.21 604.62
Intensity Change Log 2 -0.354249 0.304684 -0.037375 0.054745 0.542590 0.434108 -0.173147 0.406548 0.173929

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000367 0.000201 0.000472 0.000165 0.000105 0.000490 0.000088 0.000261 0.000196 0.000459 0.000126 0.000213
Bud Neck 0.013849 0.003876 0.006676 0.001138 0.001869 0.004984 0.000655 0.000679 0.006021 0.004866 0.001254 0.000909
Bud Site 0.006698 0.001533 0.001133 0.001586 0.001048 0.001702 0.000490 0.000904 0.003006 0.001684 0.000554 0.001246
Cell Periphery 0.000409 0.000292 0.000182 0.000755 0.000191 0.000294 0.001238 0.001369 0.000266 0.000294 0.001133 0.001060
Cytoplasm 0.001345 0.000590 0.000125 0.000123 0.000500 0.000509 0.000362 0.000057 0.000793 0.000518 0.000338 0.000090
Cytoplasmic Foci 0.067188 0.026707 0.038520 0.001561 0.028756 0.043695 0.001461 0.001069 0.042077 0.041880 0.005145 0.001316
Eisosomes 0.000030 0.000032 0.000224 0.000035 0.000018 0.000109 0.000226 0.000032 0.000022 0.000101 0.000226 0.000034
Endoplasmic Reticulum 0.000910 0.000200 0.000065 0.000219 0.000252 0.000352 0.000235 0.000341 0.000480 0.000336 0.000218 0.000280
Endosome 0.012453 0.007176 0.000566 0.002004 0.012274 0.007928 0.000461 0.001828 0.012336 0.007848 0.000472 0.001916
Golgi 0.043803 0.017943 0.002847 0.010021 0.027308 0.013286 0.001400 0.019607 0.033026 0.013783 0.001544 0.014791
Lipid Particles 0.002669 0.010084 0.004977 0.004940 0.009028 0.026087 0.009061 0.005904 0.006824 0.024377 0.008655 0.005420
Mitochondria 0.007431 0.012177 0.031343 0.013996 0.002652 0.041299 0.011079 0.011371 0.004309 0.038187 0.013094 0.012690
Mitotic Spindle 0.014316 0.005947 0.011730 0.001080 0.002404 0.003658 0.000059 0.000205 0.006533 0.003903 0.001219 0.000644
None 0.000493 0.001024 0.001968 0.000573 0.000213 0.001730 0.001045 0.000465 0.000310 0.001654 0.001137 0.000519
Nuclear Periphery 0.031877 0.010915 0.020671 0.006114 0.003270 0.012285 0.003746 0.003652 0.013186 0.012138 0.005429 0.004889
Nuclear Periphery Foci 0.037072 0.019929 0.012885 0.004535 0.019901 0.028421 0.002779 0.002912 0.025852 0.027514 0.003784 0.003728
Nucleolus 0.035025 0.015483 0.138566 0.005003 0.003435 0.036127 0.009518 0.002119 0.014385 0.033922 0.022346 0.003568
Nucleus 0.017102 0.001706 0.006133 0.000550 0.000532 0.004208 0.001400 0.000314 0.006276 0.003941 0.001870 0.000433
Peroxisomes 0.001655 0.001176 0.004474 0.000118 0.001496 0.003406 0.000289 0.000054 0.001551 0.003168 0.000705 0.000086
Vacuole 0.242195* 0.200552* 0.160583 0.295209* 0.253603* 0.210255* 0.342640* 0.299590* 0.249649* 0.209218* 0.324542* 0.297389*
Vacuole Periphery 0.463114* 0.662458* 0.555861* 0.650275* 0.631144* 0.559175* 0.611767* 0.647270* 0.572903* 0.570210* 0.606209* 0.648780*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.70 -0.86 2.40 0.63 5.02 -2.94 0.55 -1.97 1.52 -2.35 -2.16 2.02 -0.26 2.02 -2.19
Bud Neck 3.61 2.83 4.75 2.28 5.43 -4.94 2.26 2.18 12.02 -0.28 1.55 4.52 4.81 11.42 2.37
Bud Site 2.26 2.44 2.25 -0.03 -0.74 -1.28 1.04 0.29 2.99 -1.36 1.66 2.75 2.05 1.36 -2.01
Cell Periphery 0.81 1.58 -1.78 -3.83 -5.17 -3.68 -8.45 -7.25 -6.66 -0.66 -0.41 -7.03 -7.07 -7.79 0.48
Cytoplasm 1.92 3.38 3.38 1.68 0.00 0.01 0.28 1.75 7.15 1.05 1.56 1.41 3.48 6.57 0.96
Cytoplasmic Foci 2.63 2.03 4.54 3.54 7.62 -1.95 3.84 3.90 15.28 2.19 0.23 5.34 5.89 15.54 6.95
Eisosomes -0.01 -7.51 -0.39 -0.43 7.63 -8.11 -9.85 -2.27 7.63 9.45 -7.97 -10.68 -2.24 7.52 10.34
Endoplasmic Reticulum 1.16 1.40 1.13 -0.45 -6.05 -0.96 0.19 -0.83 0.30 -2.51 0.63 1.19 0.90 1.77 -1.97
Endosome 1.20 3.26 2.85 2.07 -3.83 1.44 3.97 3.50 9.45 -5.91 1.89 5.13 4.49 9.26 -6.45
Golgi 2.20 3.74 3.04 1.99 -5.23 2.77 5.19 1.43 -2.94 -9.11 3.77 6.30 3.52 -0.69 -10.91
Lipid Particles -2.77 -1.97 -2.13 1.96 -0.00 -5.22 0.01 1.14 10.89 4.90 -7.20 -0.94 0.79 11.29 5.66
Mitochondria -0.80 -4.93 -1.87 -0.35 4.20 -13.57 -7.47 -5.17 9.47 -0.17 -12.15 -6.00 -5.11 9.15 0.26
Mitotic Spindle 1.15 0.47 2.13 1.35 2.19 -0.51 1.09 1.02 6.46 -1.43 1.07 2.07 2.27 4.77 0.90
None -1.93 -8.79 -0.46 1.89 11.37 -19.69* -20.59 -8.10 16.31 14.02 -16.06 -14.92 -4.15 15.28 15.46
Nuclear Periphery 2.55 1.29 3.26 2.24 5.28 -8.79 -0.63 -0.46 10.16 0.18 0.27 2.65 2.83 9.24 1.03
Nuclear Periphery Foci 1.43 2.22 3.03 3.45 5.63 -1.41 3.07 3.04 12.68 -0.39 -0.34 4.23 4.23 12.75 0.08
Nucleolus 2.36 -7.07 4.17 1.94 11.23 -11.42 -5.19 1.31 12.50 9.91 -4.82 -2.08 3.95 12.08 12.15
Nucleus 1.54 1.14 1.65 1.93 5.38 -7.37 -3.84 1.27 8.19 6.51 0.74 1.29 1.67 8.15 7.78
Peroxisomes 1.25 -3.25 3.53 3.28 7.62 -2.72 1.95 2.33 9.50 7.18 -2.83 2.13 3.55 9.69 8.95
Vacuole 2.11 4.46 -2.80 -6.90 -13.73 2.97 -6.08 -3.09 -13.87 6.63 3.60 -6.36 -4.08 -16.78 5.11
Vacuole Periphery -5.46 -2.90 -6.11 0.50 -5.15 3.09 0.83 -0.74 -9.14 -4.96 -0.12 -2.01 -4.19 -9.32 -7.18