Standard name
Human Ortholog
Description bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0.07 0.13 0.11 0.15 0.2 0.25 0.24 0.27 0.29 0.31 – 0.08 0.11 0.1 0.1 0.15 0.06 0 0 0.05 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome – 0 0 0 0 0.05 0.05 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.31 0 0.07 0 0.17 0.2 0.25 0.67 0.53 0.63 0.58 – 0 0 0.1 0 0 0 0 0 0.07 0.14 0.12
Nucleus – 0.88 0.91 0.8 0.79 0.61 0.57 0.43 0.29 0.31 0.23 0.21 – 0.88 0.72 0.79 0.82 0.78 0.69 0.87 0.82 0.58 0.43 0.29
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0.1
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0.05 0.06 0 0.06 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane – 0 0 0 0 0.11 0.05 0.11 0 0.05 0 0.05 – 0.05 0.07 0.08 0.06 0.08 0.1 0.05 0.06 0.12 0.17 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 2 0 1
Bud – 0 0 0 1 2 4 4 12 2 12 17 – 0 0 0 0 0 0 0 2 1 1 4
Bud Neck – 0 0 0 1 1 3 6 3 5 5 7 – 0 0 0 0 0 0 0 0 0 0 2
Bud Site – 1 0 1 1 1 2 2 8 2 3 3 – 0 0 0 0 0 – – – – – –
Cell Periphery – 0 1 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm – 5 26 31 32 48 69 108 86 106 107 119 – 5 13 15 13 19 2 2 5 3 1 3
Endoplasmic Reticulum – 0 1 0 0 0 0 0 0 0 0 0 – 0 0 7 3 6 1 0 1 0 1 1
Endosome – 0 2 4 7 16 16 13 3 7 4 3 – 0 7 4 4 1 0 3 1 0 3 3
Golgi – 0 0 1 0 0 0 2 0 0 0 0 – 0 0 1 0 0 0 0 1 0 2 4
Mitochondria – 50 3 17 8 55 69 109 237 207 234 225 – 0 4 15 4 2 1 0 8 4 19 15
Nucleus – 143 326 193 235 193 192 186 105 120 85 82 – 56 83 120 103 99 24 145 303 36 62 35
Nuclear Periphery – 2 2 0 1 0 0 0 1 2 0 1 – 0 1 6 1 0 0 1 0 1 3 1
Nucleolus – 0 0 8 8 10 4 2 1 2 4 6 – 0 3 2 3 1 1 1 10 2 12 11
Peroxisomes – 0 0 0 0 2 0 0 0 0 0 0 – 0 1 0 0 0 0 0 1 0 3 0
SpindlePole – 0 1 4 8 10 17 26 11 22 19 9 – 1 2 0 1 1 0 0 5 0 3 5
Vac/Vac Membrane – 5 4 8 13 36 17 48 13 19 15 21 – 3 8 12 7 10 3 8 20 7 25 21
Unique Cell Count 163 359 241 296 319 337 431 356 392 371 390 64 116 152 126 127 35 167 371 62 146 123
Labelled Cell Count 206 366 267 315 374 393 506 480 494 488 493 65 122 182 139 140 35 167 371 62 146 123


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 4.4 6.8 6.4 6.4 5.1 4.7 4.9 3.6 4.0 3.7 3.7 – 6.4 6.6 5.5 7.3 8.1 5.4 7.0 6.8
Std Deviation (1e-4) – 0.7 1.6 1.9 2.0 1.8 1.5 1.5 1.3 1.4 1.2 0.8 – 0.9 6.6 1.2 1.5 1.8 0.5 1.9 2.3
Intensity Change (Log2) – – – -0.09 -0.08 -0.42 -0.52 -0.46 -0.89 -0.74 -0.86 -0.87 – -0.08 -0.04 -0.31 0.11 0.25 -0.32 0.05 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.2705 7.373 7.4796 7.0507 7.6303 7.6193 8.164 10.1881 9.3733 7.2805 8.6779 8.796 5.2558 6.9195 6.3027 6.1836 6.1556 5.6639
Actin 0.0136 0 0.0044 0 0 0.0167 0.085 0.0909 0.0023 0.0571 0.0007 0.0006 0.008 0 0.0145 0 0 0.0125
Bud 0.0004 0.0007 0.0012 0.0001 0 0.0003 0.0013 0.0008 0.0001 0.0011 0.0001 0.0003 0.0005 0.0001 0.0002 0.0001 0.0001 0.0002
Bud Neck 0.0009 0.0002 0.0006 0.0005 0.0005 0.0049 0.0027 0.0003 0.002 0.0003 0.0005 0.0012 0.0005 0.0003 0.0014 0.0004 0.0005 0.0014
Bud Periphery 0.0008 0.0006 0.0031 0.0001 0.0001 0.0006 0.0025 0.0007 0.0001 0.0008 0.0001 0.0008 0.0008 0.0001 0.0002 0.0002 0.0002 0.0006
Bud Site 0.0024 0.0014 0.0027 0.0001 0.0001 0.0014 0.0066 0.0043 0.0003 0.0025 0.0002 0.0005 0.0012 0.0005 0.0048 0.0002 0.0002 0.0008
Cell Periphery 0.0005 0.0002 0.0004 0 0 0.0004 0.0006 0.0002 0.0001 0.0001 0.0001 0.0003 0.0004 0.0001 0.0003 0.0001 0.0001 0.0004
Cytoplasm 0.0019 0.0002 0.0005 0.0478 0.0001 0.002 0.0218 0.0026 0.004 0.005 0.041 0.0003 0.0112 0.0001 0.0211 0.0001 0 0.0023
Cytoplasmic Foci 0.0128 0 0.0047 0.0002 0 0.0032 0.0075 0.0079 0.0104 0.0348 0.0033 0.0003 0.0078 0 0.017 0 0 0.0069
Eisosomes 0.0002 0 0.0001 0 0 0.0001 0.0006 0.0003 0 0.0001 0 0 0.0002 0 0.0001 0 0 0.0004
Endoplasmic Reticulum 0.0016 0 0.0002 0.0003 0 0.0022 0.0039 0.0066 0.0018 0.0016 0.0052 0.0006 0.0037 0 0.0046 0 0 0.0011
Endosome 0.012 0 0.0107 0.0008 0 0.0057 0.0131 0.0166 0.0201 0.0346 0.0391 0.0032 0.0166 0 0.0046 0.0001 0 0.0093
Golgi 0.0043 0 0.0012 0 0 0.0012 0.0066 0.0194 0.0087 0.0366 0.0006 0.0005 0.0035 0 0.002 0 0 0.0088
Lipid Particles 0.0118 0 0.0029 0 0 0.0027 0.003 0.0043 0.001 0.0035 0.0003 0.0004 0.0125 0 0.0043 0.0001 0 0.003
Mitochondria 0.0048 0.0003 0.0021 0.0001 0.0002 0.0088 0.005 0.0051 0.0008 0.006 0.0018 0.007 0.0084 0.0003 0.0011 0.0006 0.0005 0.0043
None 0.0038 0 0.0011 0.0003 0 0.0008 0.0049 0.0007 0.0001 0.0011 0.0039 0.0001 0.0106 0 0.0131 0 0 0.008
Nuclear Periphery 0.0055 0.0024 0.0005 0.0005 0.0019 0.0025 0.0192 0.0033 0.0013 0.0344 0.0297 0.002 0.0169 0.0006 0.0099 0.0042 0.0005 0.0026
Nucleolus 0.2299 0.1418 0.3088 0.3082 0.195 0.2256 0.0928 0.0101 0.3012 0.0116 0.1135 0.2421 0.1917 0.2173 0.2521 0.1578 0.2127 0.2418
Nucleus 0.6672 0.8498 0.6482 0.6375 0.8013 0.7106 0.6755 0.8222 0.6437 0.7497 0.7488 0.7164 0.6748 0.7794 0.6335 0.8348 0.7846 0.6784
Peroxisomes 0.0049 0 0.0029 0 0 0.0045 0.0029 0.0021 0.0005 0.0147 0.0002 0.0001 0.0014 0 0.0088 0 0 0.002
Punctate Nuclear 0.0102 0.0006 0.0011 0.0025 0.0006 0.004 0.0353 0.0008 0.0006 0.0019 0.0067 0.0191 0.021 0.0004 0.0036 0.0003 0.0002 0.0017
Vacuole 0.0085 0.0011 0.0023 0.0007 0.0001 0.0014 0.0061 0.0004 0.0005 0.0012 0.0028 0.0029 0.0053 0.0004 0.0014 0.0006 0.0003 0.009
Vacuole Periphery 0.002 0.0004 0.0005 0.0001 0.0001 0.0005 0.0031 0.0004 0.0003 0.0013 0.0016 0.0014 0.0028 0.0002 0.0014 0.0005 0.0001 0.0045

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 599.9599 664.5797 650.6077 569.8869 519.992 684.3577 719.9948 594.3563 621.1213 591.2013
Translational Efficiency 0.2173 0.2367 0.1869 0.2134 0.2198 0.3864 0.2566 0.3193 0.2594 0.2068

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
156 672 1332 188 1421 2138 2305 863 1577 2810 3637 1051

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 696.91 711.41 1052.69 950.83 700.76 860.03 992.47 955.22 700.38 824.49 1014.52 954.43
Standard Deviation 123.61 152.97 159.94 143.45 98.49 123.79 143.73 151.16 101.26 145.86 152.65 149.82
Intensity Change Log 2 — 0.029709 0.595036 0.448215 — 0.295467 0.502103 0.446913 — 0.169063 0.549190 0.447562

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000590 0.000524 0.000964 0.011403 0.000119 0.000206 0.000676 0.001183 0.000166 0.000282 0.000781 0.003011
Bud Neck 0.001420 0.004521 0.024492 0.019057 0.002420 0.005311 0.048936 0.037045 0.002321 0.005122 0.039984 0.033828
Bud Site 0.000728 0.001974 0.002333 0.021958 0.000192 0.000798 0.004565 0.016846 0.000245 0.001079 0.003748 0.017760
Cell Periphery 0.000214 0.000285 0.000115 0.000366 0.000079 0.000162 0.000121 0.000191 0.000092 0.000192 0.000119 0.000222
Cytoplasm 0.015037 0.020157 0.002600 0.081833 0.005833 0.003437 0.008313 0.070483 0.006743 0.007436 0.006221 0.072513
Cytoplasmic Foci 0.002763 0.001228 0.000090 0.017919 0.001208 0.001786 0.000695 0.002048 0.001362 0.001652 0.000473 0.004887
Eisosomes 0.000030 0.000022 0.000042 0.000083 0.000015 0.000016 0.000031 0.000040 0.000016 0.000018 0.000035 0.000047
Endoplasmic Reticulum 0.002480 0.005704 0.004481 0.006854 0.003070 0.003788 0.005697 0.008102 0.003011 0.004246 0.005251 0.007879
Endosome 0.000487 0.000606 0.000439 0.017700 0.000325 0.001227 0.001883 0.002757 0.000341 0.001079 0.001354 0.005430
Golgi 0.000211 0.000146 0.000166 0.001275 0.000446 0.000719 0.000703 0.001597 0.000423 0.000582 0.000506 0.001540
Lipid Particles 0.002337 0.000333 0.000169 0.004525 0.000597 0.001114 0.000212 0.000684 0.000769 0.000928 0.000196 0.001371
Mitochondria 0.000723 0.000852 0.001582 0.031108 0.000941 0.003535 0.002508 0.002451 0.000919 0.002893 0.002169 0.007577
Mitotic Spindle 0.001906 0.002940 0.004221 0.045754 0.001831 0.003868 0.009198 0.019753 0.001838 0.003646 0.007375 0.024404
None 0.015104 0.020375 0.003827 0.034452 0.005333 0.006137 0.003334 0.010106 0.006299 0.009542 0.003514 0.014461
Nuclear Periphery 0.000388 0.000508 0.001278 0.005676 0.000755 0.001288 0.002694 0.003899 0.000719 0.001101 0.002175 0.004217
Nuclear Periphery Foci 0.000511 0.000578 0.000680 0.007601 0.000344 0.001008 0.000792 0.001724 0.000361 0.000905 0.000751 0.002775
Nucleolus 0.032647 0.026588 0.104140 0.030849 0.024527 0.017842 0.038769 0.036009 0.025331 0.019933 0.062710 0.035086
Nucleus 0.920199* 0.910228* 0.845579* 0.568981* 0.951029* 0.944803* 0.864328* 0.759207* 0.947979* 0.936535* 0.857462* 0.725180*
Peroxisomes 0.001449 0.000436 0.000664 0.006264 0.000326 0.000936 0.000219 0.000576 0.000437 0.000816 0.000382 0.001593
Vacuole 0.000659 0.001757 0.001775 0.081258 0.000446 0.001410 0.005305 0.024174 0.000467 0.001493 0.004012 0.034385
Vacuole Periphery 0.000116 0.000238 0.000363 0.005083 0.000164 0.000609 0.001023 0.001124 0.000159 0.000520 0.000781 0.001832

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.27 -1.04 -1.63 -1.74 -1.43 -2.80 -16.86 -14.37 -13.16 -7.73 -2.11 -13.99 -5.37 -5.09 -3.31
Bud Neck -4.28 -22.52 -9.20 -8.23 -1.05 -5.06 -30.96 -17.08 -15.73 3.57 -5.98 -36.49 -18.95 -17.45 1.35
Bud Site -1.64 -3.12 -3.69 -3.37 -3.21 -3.95 -7.86 -7.71 -7.38 -5.33 -4.39 -9.48 -8.58 -8.07 -6.40
Cell Periphery -0.64 1.18 0.22 1.36 -2.41 -2.56 -2.43 -7.26 -0.60 -5.38 -3.29 -1.08 -5.41 0.31 -6.02
Cytoplasm -0.35 2.66 -3.56 -3.77 -6.38 1.98 -3.69 -11.82 -12.68 -10.72 -0.38 -0.61 -12.66 -12.69 -12.87
Cytoplasmic Foci 1.63 2.46 -0.54 -2.18 -2.94 -1.25 1.62 -1.21 0.19 -3.22 -0.66 2.78 -1.69 -1.09 -4.57
Eisosomes 1.50 -1.67 -6.96 -9.38 -7.18 -1.23 -11.59 -13.59 -13.40 -9.19 -1.00 -13.41 -15.83 -16.00 -10.79
Endoplasmic Reticulum -4.32 -4.06 -7.61 -5.51 -6.48 -2.40 -11.26 -14.63 -13.51 -9.18 -4.38 -11.48 -16.43 -14.28 -11.79
Endosome -0.23 0.42 -4.25 -4.25 -4.36 -3.76 -4.28 -8.17 -4.49 -3.56 -3.96 -4.42 -8.89 -6.29 -6.19
Golgi 0.66 0.33 -2.29 -4.03 -3.91 -0.80 -0.11 -0.67 -0.16 -0.64 -0.54 0.15 -0.78 -0.46 -0.99
Lipid Particles 1.28 1.34 0.42 -3.61 -3.88 -1.57 1.68 -3.28 -0.44 -9.88 -0.18 2.14 -1.85 -2.07 -9.42
Mitochondria -0.38 -4.63 -1.36 -1.35 -1.16 -2.98 -5.68 -5.45 0.93 0.52 -2.90 -5.37 -2.79 -0.71 -1.25
Mitotic Spindle -0.32 -0.95 -4.16 -4.09 -3.99 -1.56 -5.70 -7.36 -6.86 -4.85 -1.55 -5.52 -8.43 -7.94 -6.46
None -0.93 2.56 -0.24 0.65 -2.84 -0.65 2.47 -2.20 -1.28 -5.82 -2.39 3.44 -2.23 0.22 -5.69
Nuclear Periphery -0.89 -2.42 -5.46 -5.26 -4.22 -2.48 -7.87 -8.02 -6.78 -2.99 -2.31 -7.61 -9.25 -8.16 -4.94
Nuclear Periphery Foci -0.18 -0.78 -2.99 -2.98 -2.84 -2.03 -2.91 -6.09 -4.37 -4.92 -2.14 -3.07 -6.71 -5.38 -5.68
Nucleolus 0.66 -9.93 -0.66 -1.49 8.91 3.01 -5.75 -2.23 -4.45 2.37 2.42 -14.62 -2.50 -4.44 9.74
Nucleus 0.38 6.01 11.15 12.20 8.82 1.51 21.26 25.18 24.26 13.43 2.75 24.58 27.97 26.72 15.66
Peroxisomes 1.27 1.00 0.01 -1.83 -1.43 -2.62 0.78 -2.79 1.04 -4.26 -1.68 0.26 -2.08 -0.26 -2.90
Vacuole -2.27 -5.00 -8.46 -8.36 -8.33 -2.94 -10.03 -13.22 -12.98 -11.22 -3.74 -10.98 -15.56 -15.30 -14.13
Vacuole Periphery -2.89 -4.61 -2.53 -2.32 -2.10 -4.58 -4.70 -5.15 -0.99 0.10 -4.84 -5.51 -5.32 -2.42 -1.70
Standard name
Human Ortholog
Description bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Gcn4

Gcn4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcn4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available