ORF
Description Putative protein of unknown function; conserved among S. cerevisiae strains; YEL008W is not an essential gene; predicted to be involved in metabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.96 0.97 0.93 – 0.85 0.79 0.77 0.68 0.78 0.99 1.0 0.99 0.91 0.91 0.96 0.94 0.92 0.96 0.95 0.92 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.11 0.07 0.28 – 0.35 0.55 0.41 0.56 0.55 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1 3
Bud 1 0 3 1 2 0 – 2 3 2 6 7 0 2 0 1 1 0 0 1 0 0 4 14
Bud Neck 0 0 0 1 2 0 – 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 – 0 0 1 1 1 1 1 0 6 1 0 0 0 0 0 0 0
Cytoplasm 167 170 58 136 221 161 – 280 158 246 148 282 391 488 681 78 79 48 164 161 66 93 333 404
Endoplasmic Reticulum 1 0 0 0 1 1 – 0 1 2 2 0 0 2 0 5 3 2 0 0 0 0 0 2
Endosome 0 0 0 0 0 0 – 0 0 0 1 0 2 0 1 1 3 0 1 2 0 0 2 4
Golgi 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 2
Mitochondria 5 0 1 15 15 49 – 116 109 130 122 197 0 2 1 5 4 2 1 1 0 1 7 5
Nucleus 0 0 0 0 0 2 – 2 0 3 2 2 1 2 2 0 2 1 0 1 0 0 2 3
Nuclear Periphery 1 0 0 0 0 0 – 1 1 1 0 0 1 0 2 1 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 – 2 0 4 0 2 0 0 0 1 0 0 1 0 0 0 1 3
Vac/Vac Membrane 0 0 0 0 1 1 – 1 3 0 0 4 7 0 5 1 3 0 2 0 0 0 3 7
Unique Cell Count 172 171 59 141 228 174 329 199 318 217 361 396 490 689 86 87 50 175 175 70 98 362 465
Labelled Cell Count 176 171 62 153 243 214 405 275 389 283 497 403 497 693 102 97 53 175 175 70 98 362 465


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.2 3.1 3.6 4.0 3.3 – 3.2 3.2 3.0 2.9 2.9 5.8 5.7 5.5 5.4 5.5 5.4 4.7 5.1 5.9
Std Deviation (1e-4) 0.6 0.8 0.5 1.3 1.3 1.3 – 1.0 2.0 0.9 1.2 1.1 6.4 1.4 1.5 1.1 1.4 1.3 1.0 1.3 1.9
Intensity Change (Log2) – – – 0.18 0.35 0.09 – 0.04 0.0 -0.08 -0.13 -0.11 0.87 0.85 0.8 0.77 0.8 0.78 0.59 0.71 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -0.6 -1.6 – -2.8 -3.5 -3.7 -4.7 -3.7 0.3 1.3 0.4 -1.9 -1.8 -0.7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yel008w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available