ORF | |
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Description | Putative protein of unknown function; conserved among S. cerevisiae strains; YEL008W is not an essential gene; predicted to be involved in metabolism |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 |
Bud | 1 | 0 | 3 | 1 | 2 | 0 | – | 2 | 3 | 2 | 6 | 7 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 14 |
Bud Neck | 0 | 0 | 0 | 1 | 2 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 167 | 170 | 58 | 136 | 221 | 161 | – | 280 | 158 | 246 | 148 | 282 | 391 | 488 | 681 | 78 | 79 | 48 | 164 | 161 | 66 | 93 | 333 | 404 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 1 | 1 | – | 0 | 1 | 2 | 2 | 0 | 0 | 2 | 0 | 5 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 3 | 0 | 1 | 2 | 0 | 0 | 2 | 4 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Mitochondria | 5 | 0 | 1 | 15 | 15 | 49 | – | 116 | 109 | 130 | 122 | 197 | 0 | 2 | 1 | 5 | 4 | 2 | 1 | 1 | 0 | 1 | 7 | 5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | – | 2 | 0 | 3 | 2 | 2 | 1 | 2 | 2 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 2 | 3 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | – | 2 | 0 | 4 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 3 | 0 | 0 | 4 | 7 | 0 | 5 | 1 | 3 | 0 | 2 | 0 | 0 | 0 | 3 | 7 |
Unique Cell Count | 172 | 171 | 59 | 141 | 228 | 174 | 329 | 199 | 318 | 217 | 361 | 396 | 490 | 689 | 86 | 87 | 50 | 175 | 175 | 70 | 98 | 362 | 465 | |
Labelled Cell Count | 176 | 171 | 62 | 153 | 243 | 214 | 405 | 275 | 389 | 283 | 497 | 403 | 497 | 693 | 102 | 97 | 53 | 175 | 175 | 70 | 98 | 362 | 465 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 4.3 | 5.2 | 3.1 | 3.6 | 4.0 | 3.3 | – | 3.2 | 3.2 | 3.0 | 2.9 | 2.9 | 5.8 | 5.7 | 5.5 | 5.4 | 5.5 | 5.4 | 4.7 | 5.1 | 5.9 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.5 | 1.3 | 1.3 | 1.3 | – | 1.0 | 2.0 | 0.9 | 1.2 | 1.1 | 6.4 | 1.4 | 1.5 | 1.1 | 1.4 | 1.3 | 1.0 | 1.3 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.35 | 0.09 | – | 0.04 | 0.0 | -0.08 | -0.13 | -0.11 | 0.87 | 0.85 | 0.8 | 0.77 | 0.8 | 0.78 | 0.59 | 0.71 | 0.9 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | -0.6 | -1.6 | – | -2.8 | -3.5 | -3.7 | -4.7 | -3.7 | 0.3 | 1.3 | 0.4 | -1.9 | -1.8 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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