Standard name | |
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Description | Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 3 | 4 |
Bud | 3 | 3 | 1 | 6 | 8 | 17 | 10 | – | 9 | 22 | 8 | 19 | 1 | 2 | 0 | 1 | 1 | 0 | 4 | 3 | 3 | 7 | 10 | 21 |
Bud Neck | 2 | 13 | 4 | 7 | 12 | 19 | 10 | – | 17 | 16 | 14 | 16 | 4 | 4 | 2 | 0 | 1 | 1 | 1 | 6 | 2 | 4 | 3 | 8 |
Bud Site | 11 | 6 | 0 | 8 | 5 | 21 | 6 | – | 18 | 39 | 16 | 35 | 2 | 3 | 0 | 0 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 10 | 20 | 20 | 24 | 12 | 26 | – | 14 | 20 | 12 | 13 | 89 | 71 | 88 | 15 | 14 | 19 | 0 | 2 | 4 | 21 | 31 | 65 |
Endoplasmic Reticulum | 0 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 41 | 19 | 36 | 18 | 23 | 6 | 22 | – | 28 | 20 | 19 | 9 | 108 | 99 | 157 | 38 | 76 | 90 | 13 | 23 | 15 | 30 | 35 | 53 |
Golgi | 0 | 0 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 5 | 1 | 5 | 0 | 5 | 4 |
Mitochondria | 25 | 22 | 3 | 2 | 3 | 30 | 5 | – | 22 | 34 | 16 | 20 | 2 | 3 | 3 | 0 | 3 | 6 | 4 | 6 | 7 | 6 | 8 | 14 |
Nucleus | 9 | 27 | 10 | 13 | 27 | 6 | 10 | – | 16 | 17 | 14 | 21 | 15 | 13 | 10 | 2 | 3 | 2 | 2 | 10 | 3 | 3 | 2 | 4 |
Nuclear Periphery | 0 | 4 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 |
Nucleolus | 212 | 244 | 119 | 103 | 128 | 114 | 133 | – | 208 | 218 | 159 | 202 | 118 | 82 | 86 | 12 | 38 | 51 | 5 | 39 | 11 | 5 | 8 | 15 |
Peroxisomes | 38 | 15 | 7 | 11 | 14 | 4 | 17 | – | 39 | 24 | 17 | 13 | 66 | 68 | 123 | 10 | 11 | 31 | 3 | 6 | 0 | 2 | 2 | 1 |
SpindlePole | 377 | 453 | 368 | 330 | 425 | 238 | 354 | – | 414 | 532 | 287 | 363 | 275 | 186 | 227 | 51 | 118 | 124 | 379 | 462 | 386 | 282 | 358 | 308 |
Vac/Vac Membrane | 1 | 3 | 2 | 2 | 1 | 0 | 0 | – | 1 | 0 | 0 | 2 | 6 | 3 | 8 | 8 | 9 | 3 | 1 | 3 | 1 | 3 | 5 | 10 |
Unique Cell Count | 421 | 553 | 424 | 375 | 499 | 306 | 426 | 536 | 655 | 361 | 473 | 440 | 348 | 426 | 88 | 171 | 194 | 426 | 576 | 449 | 376 | 483 | 528 | |
Labelled Cell Count | 725 | 822 | 573 | 520 | 672 | 467 | 593 | 786 | 942 | 562 | 715 | 686 | 535 | 705 | 141 | 281 | 336 | 426 | 576 | 449 | 376 | 483 | 528 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 5.6 | 6.4 | 5.8 | 6.5 | 4.5 | 5.5 | – | 5.4 | 5.3 | 5.6 | 5.4 | 5.9 | 6.1 | 6.1 | 7.1 | 7.7 | 7.0 | 4.9 | 5.2 | 5.3 |
Std Deviation (1e-4) | 1.1 | 1.1 | 1.5 | 1.2 | 1.6 | 1.3 | 1.4 | – | 1.3 | 1.3 | 1.3 | 1.4 | 1.5 | 1.7 | 1.7 | 1.5 | 1.8 | 1.6 | 1.6 | 1.6 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.15 | 0.01 | -0.52 | -0.24 | – | -0.26 | -0.29 | -0.2 | -0.24 | -0.12 | -0.08 | -0.08 | 0.14 | 0.26 | 0.13 | -0.38 | -0.3 | -0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 2.7 | – | 0 | 3.5 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 1.1 | 1.7 | 0 | 1.6 | – | 2.3 | 1.8 | 2.7 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 3.8 | 5.1 | 4.4 | 5.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4 | 0.1 | -0.5 | 0.9 | – | -1.8 | -1.4 | -1.0 | -1.6 | 6.9 | 6.7 | 7.0 | 4.2 | 1.7 | 2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.1 | -2.4 | 0 | -1.9 | – | -2.0 | -3.9 | -1.8 | -4.5 | 6.3 | 7.3 | 9.9 | 8.4 | 10.2 | 10.9 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 3.3 | 4.0 | 3.4 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.9 | 2.4 | -0.4 | 0 | – | 0.6 | 0.2 | 1.2 | 1.7 | 0.9 | 1.1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.2 | -0.8 | 0 | 1.0 | – | 3.5 | 1.8 | 4.7 | 4.6 | -0.4 | -1.4 | -2.7 | -2.8 | -1.5 | -0.5 |
Peroxisomes | 1.2 | 1.2 | 0 | 2.1 | – | 4.1 | 1.9 | 2.5 | 1.1 | 7.1 | 8.4 | 11.0 | 0 | 3.1 | 6.9 |
SpindlePole | 0.5 | -0.7 | 0 | -1.5 | – | -3.8 | -2.4 | -2.7 | -3.9 | -8.2 | -10.2 | -10.7 | -6.4 | -5.1 | -6.6 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.954 | 1.3141 | 1.4438 | 1.3682 | 1.1777 | 1.366 | 3.8442 | 2.5176 | 2.7451 | 2.9722 | 2.5136 | 2.4721 | 5.5892 | 5.9342 | 5.8532 | 6.936 | 5.698 | 5.3424 |
Actin | 0.0163 | 0 | 0.0007 | 0.003 | 0.0012 | 0.0038 | 0.0314 | 0 | 0 | 0.0037 | 0 | 0.0028 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0005 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0024 | 0.0008 | 0.0024 | 0.0005 | 0.0007 | 0.0005 | 0.0038 | 0.0005 | 0.0005 | 0.0392 | 0.0002 | 0.0003 | 0.0003 | 0.0009 | 0.0001 | 0.0016 | 0.0003 | 0.0002 |
Bud Periphery | 0.0009 | 0 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 |
Bud Site | 0.0017 | 0.0026 | 0.0004 | 0.0024 | 0.001 | 0.0002 | 0.0015 | 0.0008 | 0.0003 | 0.0032 | 0.0001 | 0.0001 | 0.0001 | 0.0024 | 0.0002 | 0.0001 | 0.0001 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0054 | 0.0003 | 0.002 | 0.0049 | 0.003 | 0.0001 | 0.0025 | 0.0001 | 0 | 0.0014 | 0 | 0.0001 | 0 | 0.0016 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0239 | 0.0082 | 0.0053 | 0.0311 | 0.0821 | 0.002 | 0.0162 | 0.0024 | 0.0023 | 0.0124 | 0.0127 | 0.0019 | 0.0022 | 0.0079 | 0.0013 | 0.0299 | 0.0273 | 0.0007 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0018 | 0 | 0.0007 | 0.0013 | 0.0009 | 0.0002 | 0.0085 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0 |
Endosome | 0.0237 | 0.0001 | 0.0063 | 0.0138 | 0.0253 | 0.002 | 0.0167 | 0.0004 | 0.0004 | 0.013 | 0.0002 | 0.0012 | 0 | 0.0029 | 0 | 0.0003 | 0.0002 | 0 |
Golgi | 0.0068 | 0.0001 | 0.0011 | 0.0046 | 0.0031 | 0.0016 | 0.0068 | 0.0002 | 0.0001 | 0.0035 | 0.0003 | 0.0011 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0006 | 0 |
Lipid Particles | 0.0184 | 0.0011 | 0.0055 | 0.0142 | 0.0478 | 0.0021 | 0.0154 | 0.0023 | 0.0013 | 0.011 | 0.0036 | 0.0023 | 0.0004 | 0.0048 | 0.0001 | 0.0044 | 0.0115 | 0.0001 |
Mitochondria | 0.0058 | 0.0001 | 0.0045 | 0.0049 | 0.0009 | 0.0026 | 0.003 | 0 | 0.0001 | 0.0012 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 |
None | 0.0052 | 0.0003 | 0.0029 | 0.0065 | 0.0024 | 0.0001 | 0.0041 | 0 | 0 | 0.0017 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0105 | 0.0004 | 0.0044 | 0.0021 | 0.0088 | 0.0031 | 0.021 | 0.0005 | 0.0003 | 0.0035 | 0.0001 | 0.0004 | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0.0002 | 0.0001 |
Nucleolus | 0.0869 | 0.1092 | 0.0586 | 0.0255 | 0.0465 | 0.0479 | 0.0165 | 0.0981 | 0.0612 | 0.0731 | 0.0109 | 0.0224 | 0.0079 | 0.0931 | 0.0717 | 0.0206 | 0.0397 | 0.0491 |
Nucleus | 0.039 | 0.0726 | 0.0233 | 0.0341 | 0.0045 | 0.0123 | 0.0336 | 0.0679 | 0.0205 | 0.037 | 0.0008 | 0.009 | 0.0027 | 0.0501 | 0.0498 | 0.0044 | 0.0017 | 0.0123 |
Peroxisomes | 0.0058 | 0.0002 | 0.0012 | 0.0055 | 0.0055 | 0.0005 | 0.0043 | 0.0002 | 0.0001 | 0.0025 | 0.0002 | 0.0016 | 0.0001 | 0.0003 | 0 | 0.0004 | 0.0006 | 0 |
Punctate Nuclear | 0.7392 | 0.8038 | 0.8787 | 0.8347 | 0.7647 | 0.9205 | 0.8106 | 0.8265 | 0.9118 | 0.7894 | 0.9709 | 0.9562 | 0.9861 | 0.816 | 0.8764 | 0.9373 | 0.9177 | 0.9374 |
Vacuole | 0.0038 | 0.0001 | 0.0008 | 0.0084 | 0.0006 | 0.0002 | 0.0014 | 0.0001 | 0.0008 | 0.0016 | 0 | 0.0001 | 0 | 0.0166 | 0 | 0 | 0 | 0 |
Vacuole Periphery | 0.0019 | 0 | 0.0007 | 0.0013 | 0.0005 | 0.0003 | 0.0011 | 0 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0.0006 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.6798 | 22.2157 | 24.178 | 11.089 | 32.221 | 9.9351 | 32.1959 | 17.6873 | 19.5204 | 24.3987 |
Translational Efficiency | 0.7841 | 0.9145 | 0.2255 | 0.6206 | 0.5618 | 2.2923 | 0.6679 | 0.9689 | 0.6113 | 0.7711 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |