TheCellVision

YAT2

Standard name	YAT2 ORF YER024W Aliases carnitine O-acetyltransferase YAT2
Human Ortholog
Description	Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane

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Scale images

YAT2

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0.05	0	0.05	0	0	0	0	0	0
Cytoplasm	0.98	–	0.94	0.99	0.98	0.94	0.9	0.85	0.77	–	0.65	0.65	0.99	1.0	0.97	0.97	0.97	0.93	0.95	0.89	0.89	0.96	0.91	0.87
Endoplasmic Reticulum	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0.06	0	0	0	0	0	0	0	0
Endosome	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	–	0.1	0.09	0	0.22	0.19	0.25	0.46	–	0.52	0.53	0	0	0.05	0	0	0	0	0	0.05	0	0	0
Nucleus	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	–	0	0	0	0	0	0	0	–	0	0.07	0	0	0	0	0	0	0	0	0	0	0	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	–	0	0	0	1	0	0	1	–	1	0	0	0	0	0	0	0	0	0	0	0	1	4
Bud	1	–	3	2	3	6	5	8	15	–	7	15	0	1	16	0	0	0	0	0	0	0	1	6
Bud Neck	0	–	0	0	0	1	1	1	0	–	0	0	0	0	0	0	0	0	0	0	1	0	0	3
Bud Site	0	–	0	0	0	0	0	2	0	–	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	–	1	2	16	5	12	10	4	–	7	11	2	10	11	14	6	11	0	0	0	0	0	0
Cytoplasm	56	–	148	374	542	361	720	884	278	–	170	366	156	469	487	292	166	222	67	35	146	111	264	356
Endoplasmic Reticulum	0	–	1	1	3	0	5	3	0	–	3	5	1	0	2	18	4	5	0	0	1	0	3	9
Endosome	0	–	0	0	0	1	0	5	0	–	0	0	0	0	1	0	0	4	0	0	0	1	1	5
Golgi	0	–	0	1	0	0	0	0	0	–	0	0	0	0	1	1	0	0	0	0	0	0	0	5
Mitochondria	2	–	15	35	19	83	150	260	166	–	136	300	0	4	27	7	3	0	0	0	7	0	1	3
Nucleus	0	–	1	0	0	1	2	10	1	–	1	5	1	0	0	3	2	2	0	0	2	0	1	0
Nuclear Periphery	0	–	0	0	0	0	3	1	0	–	3	21	0	0	1	0	0	1	0	0	0	0	0	0
Nucleolus	0	–	0	0	0	0	0	1	0	–	0	0	0	0	1	0	0	1	0	0	0	0	0	0
Peroxisomes	0	–	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	1
SpindlePole	0	–	0	0	0	0	1	3	1	–	0	1	0	0	0	0	0	0	0	0	0	0	0	4
Vac/Vac Membrane	1	–	1	0	1	2	3	15	4	–	11	41	0	0	1	6	4	7	0	0	2	2	8	5
Unique Cell Count	57		157	379	554	384	797	1041	363		260	564	158	469	503	301	172	238	71	40	165	117	292	412
Labelled Cell Count	60		170	415	584	461	902	1203	470		339	765	160	484	548	341	185	253	71	40	165	117	292	412

Yeast GFP Assignment

Cytoplasm

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	8.9	–	4.3	4.1	5.3	4.4	4.0	3.9	3.5	–	3.8	4.2	6.5	6.4	5.9	9.9	10.2	11.1	6.6	6.4	7.0
Std Deviation (1e^-4)	2.1	–	0.7	0.9	1.0	1.1	1.1	0.9	1.1	–	1.4	1.7	1.3	1.7	1.6	2.0	2.7	2.9	1.4	1.6	2.5
Intensity Change (Log₂)	–	–	–	-0.08	0.29	0.01	-0.12	-0.15	-0.32	–	-0.2	-0.05	0.58	0.57	0.45	1.2	1.23	1.36	0.6	0.57	0.7

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Cell Periphery	0	0	0	0	0	0	–	0	0	0	0	0	2.3	0	0
Cytoplasm	2.9	2.3	-0.1	-1.6	-3.2	-4.8	–	-6.7	-7.2	2.2	5.2	1.5	1.4	1.0	-0.4
Endoplasmic Reticulum	0	0	0	0	0	0	–	0	0	0	0	0	2.7	0	0
Endosome	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Mitochondria	0	-3.2	0	0	0	0	–	0	0	-4.0	0	-1.9	-3.4	-3.1	-4.9
Nucleus	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0
Vacuole	0	0	0	0	0	0	–	0	3.1	0	0	0	0	0	0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	6.5723	9.4172	7.7526	8.0878	6.195	8.6574	7.4466	12.298	10.3262	10.5252	9.5923	9.7434	4.3864	7.6114	5.6495	6.4952	4.7566	5.6005
Actin	0.0409	0.0008	0.0083	0.001	0.0003	0.0009	0.0124	0.0001	0.0115	0.0016	0.0003	0.0018	0.0205	0.0004	0.0009	0.0275	0.0149	0.0008
Bud	0.0008	0.0016	0.0039	0.0015	0.0007	0.0006	0.001	0.0002	0.0004	0.0007	0.0001	0.0003	0.0004	0.0112	0.0006	0.0004	0.0006	0.0003
Bud Neck	0.0024	0.0004	0.001	0.0011	0.0031	0.0015	0.0057	0.0002	0.0003	0.0009	0.0002	0.0023	0.001	0.0002	0.0004	0.0004	0.0009	0.0006
Bud Periphery	0.0012	0.0025	0.0039	0.0014	0.0003	0.0008	0.0011	0.0001	0.0003	0.0015	0	0.0003	0.0003	0.0025	0.0003	0.0003	0.0004	0.0002
Bud Site	0.002	0.0165	0.0048	0.0014	0.0016	0.0002	0.0036	0.001	0.0006	0.0025	0	0.0003	0.0017	0.0018	0.0011	0.0003	0.0017	0.0001
Cell Periphery	0.0016	0.0039	0.0009	0.0009	0.0003	0.0009	0.0008	0.0002	0.0002	0.0005	0	0.0001	0.0007	0.0002	0.0002	0.0001	0.0003	0.0001
Cytoplasm	0.7838	0.9232	0.9028	0.8402	0.854	0.9162	0.7576	0.9806	0.9056	0.9135	0.9809	0.921	0.8054	0.9528	0.9214	0.8969	0.8844	0.935
Cytoplasmic Foci	0.0158	0.0056	0.0137	0.0112	0.0519	0.0059	0.03	0.0031	0.0097	0.0101	0.0035	0.0095	0.0231	0.0065	0.0171	0.0126	0.0165	0.0082
Eisosomes	0.0004	0	0.0001	0	0	0	0.0001	0	0.0001	0.0001	0	0	0.0009	0	0	0.0003	0.0004	0
Endoplasmic Reticulum	0.0572	0.0147	0.0121	0.0359	0.0348	0.0315	0.0427	0.0062	0.0137	0.0047	0.0022	0.0159	0.0431	0.0034	0.013	0.0064	0.0101	0.0201
Endosome	0.0338	0.0101	0.0159	0.0531	0.0209	0.017	0.0617	0.0024	0.0142	0.0209	0.0083	0.0206	0.0327	0.0116	0.0167	0.0294	0.0207	0.0193
Golgi	0.0096	0.0014	0.0033	0.0014	0.0017	0.002	0.0213	0.0002	0.0033	0.0019	0.0002	0.0055	0.0068	0.001	0.0048	0.0048	0.0032	0.0026
Lipid Particles	0.0052	0.0004	0.0019	0.0002	0.0007	0.0029	0.01	0	0.0047	0.0019	0	0.0011	0.0133	0	0.0024	0.0054	0.0014	0.0007
Mitochondria	0.0025	0.0006	0.0009	0.0008	0.0009	0.0042	0.0131	0.0001	0.0146	0.0149	0.0001	0.0119	0.0039	0.0002	0.0066	0.0016	0.0013	0.0064
None	0.0076	0.0017	0.0071	0.0026	0.008	0.003	0.0043	0.0009	0.0047	0.0014	0.0005	0.0008	0.0164	0.0042	0.0025	0.002	0.0325	0.0012
Nuclear Periphery	0.01	0.001	0.0014	0.0037	0.0011	0.0029	0.0117	0.0004	0.0008	0.0012	0.0006	0.0022	0.0057	0.0003	0.0015	0.0011	0.0017	0.0009
Nucleolus	0.0002	0.0002	0.0002	0.0001	0.0003	0.0003	0.0001	0	0.0001	0.0005	0	0.0001	0.0013	0	0.0008	0	0.0002	0
Nucleus	0.0026	0.0011	0.0018	0.0029	0.0017	0.0013	0.0027	0.0008	0.0009	0.0017	0.0008	0.0015	0.0021	0.0005	0.0014	0.0008	0.0014	0.0006
Peroxisomes	0.0035	0	0.0013	0.0001	0.0007	0.0004	0.0053	0	0.0025	0.0006	0	0.0005	0.0067	0	0.0019	0.0008	0.0006	0.0001
Punctate Nuclear	0.0015	0.0001	0.0007	0.0003	0.0007	0.0009	0.0008	0.0001	0.0002	0.0001	0	0.0004	0.0074	0.0001	0.0003	0.0003	0.0015	0.0001
Vacuole	0.0156	0.0133	0.0126	0.035	0.0152	0.0052	0.0116	0.0031	0.0105	0.0165	0.0017	0.0031	0.0055	0.0027	0.0047	0.007	0.0043	0.0021
Vacuole Periphery	0.0021	0.0007	0.0015	0.0054	0.0011	0.0014	0.0022	0.0002	0.0012	0.0023	0.0004	0.0008	0.001	0.0004	0.0013	0.0014	0.0008	0.0007

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	6.3689	4.27	5.545	5.096	5.419	12.7307	11.2433	10.4417	9.063	8.719
Translational Efficiency	–	–	–	–	–	–	–	–	–	–

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	–

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
931	712	140	425	751	1240	939	106	1682	1952	1079	531

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	674.24	1298.03	1121.70	873.46	677.07	907.65	831.51	865.87	675.50	1050.04	869.16	871.94
Standard Deviation	116.55	350.85	226.12	112.65	138.49	182.47	95.85	133.41	126.82	318.37	155.36	117.13
Intensity Change Log ₂	—	0.944990	0.734353	0.373479	—	0.422831	0.296429	0.354845	—	0.706865	0.531488	0.364173

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000098	0.001202	0.001423	0.000878	0.000261	0.000239	0.000126	0.000115	0.000171	0.000590	0.000294	0.000726
Bud Neck	0.004650	0.002477	0.003847	0.011285	0.008172	0.004245	0.001670	0.004120	0.006223	0.003600	0.001953	0.009855
Bud Site	0.007559	0.038763	0.018444	0.064634	0.017447	0.026138	0.003470	0.006007	0.011974	0.030743	0.005413	0.052931
Cell Periphery	0.000173	0.000914	0.000511	0.000134	0.000204	0.000139	0.000129	0.000127	0.000187	0.000421	0.000178	0.000132
Cytoplasm	0.665984*	0.794631*	0.515849*	0.506143*	0.598798*	0.716382*	0.481262*	0.411848*	0.635986*	0.744923*	0.485749*	0.487319*
Cytoplasmic Foci	0.180884*	0.103831	0.062580	0.045712	0.196360*	0.164519	0.021006	0.074327	0.187794*	0.142383	0.026400	0.051425
Eisosomes	0.000095	0.000016	0.000052	0.000052	0.000096	0.000032	0.000050	0.000091	0.000095	0.000026	0.000051	0.000059
Endoplasmic Reticulum	0.000839	0.001353	0.006021	0.001607	0.002134	0.000823	0.001747	0.001274	0.001417	0.001016	0.002302	0.001540
Endosome	0.009408	0.008668	0.008284	0.011490	0.027076	0.018123	0.002577	0.009950	0.017296	0.014674	0.003318	0.011182
Golgi	0.002308	0.008572	0.001374	0.001209	0.007360	0.010037	0.000064	0.000206	0.004564	0.009503	0.000234	0.001009
Lipid Particles	0.004266	0.002335	0.004086	0.001513	0.004409	0.001193	0.001470	0.002546	0.004330	0.001609	0.001809	0.001719
Mitochondria	0.001351	0.000113	0.000272	0.000937	0.003708	0.002254	0.000448	0.000113	0.002404	0.001473	0.000425	0.000772
Mitotic Spindle	0.000095	0.002373	0.006330	0.031752	0.001236	0.002359	0.001728	0.001694	0.000604	0.002364	0.002326	0.025752
None	0.010359	0.000610	0.005972	0.006593	0.010312	0.001524	0.005158	0.000615	0.010338	0.001191	0.005264	0.005399
Nuclear Periphery	0.000208	0.000024	0.000826	0.000912	0.000267	0.000052	0.001510	0.000925	0.000235	0.000042	0.001422	0.000915
Nuclear Periphery Foci	0.000896	0.000882	0.010456	0.001247	0.000794	0.000501	0.000816	0.002049	0.000850	0.000640	0.002067	0.001407
Nucleolus	0.000583	0.000144	0.000203	0.000424	0.001301	0.000837	0.000497	0.000597	0.000903	0.000584	0.000459	0.000459
Nucleus	0.060029	0.002595	0.210444*	0.109800	0.035083	0.005020	0.399180*	0.175093*	0.048891	0.004135	0.374691*	0.122834
Peroxisomes	0.001502	0.000957	0.000468	0.011347	0.002369	0.001992	0.000178	0.000939	0.001889	0.001615	0.000215	0.009270
Vacuole	0.047516	0.026326	0.141715	0.191735	0.077763	0.040444	0.076689	0.306602*	0.061021	0.035295	0.085126	0.214666*
Vacuole Periphery	0.001197	0.003214	0.000841	0.000595	0.004850	0.003149	0.000224	0.000763	0.002828	0.003172	0.000304	0.000629

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-2.13	-9.46	-3.85	1.30	5.68	0.32	0.85	1.05	1.50	0.55	-2.18	-4.96	-3.00	0.69	-0.70
Bud Neck	2.29	0.55	-0.93	-2.51	-1.31	4.51	6.86	4.43	1.15	-0.82	4.39	6.71	0.93	-1.75	-2.73
Bud Site	-9.80	-4.14	-3.85	2.24	-1.55	-4.28	8.95	5.90	9.62	-1.34	-10.98	5.59	-2.61	2.10	-4.35
Cell Periphery	-3.18	-6.18	5.22	3.45	7.40	3.90	5.18	6.44	3.35	2.66	-2.73	1.07	7.66	3.67	5.87
Cytoplasm	-11.37	9.08	20.88	27.88	3.81	-9.86	16.38	9.91	14.78	1.72	-13.32	23.54	22.08	30.34	3.57
Cytoplasmic Foci	10.76	16.19	26.95	13.81	4.34	3.98	28.18	15.77	13.46	-5.67	8.37	39.26	33.31	24.83	-3.12
Eisosomes	12.19	2.46	4.94	-9.77	1.00	4.08	2.00	0.60	-7.42	-2.86	9.01	3.82	3.20	-12.61	-1.18
Endoplasmic Reticulum	-1.80	-6.63	-6.48	-3.44	4.82	2.79	-2.01	0.71	-2.90	3.86	1.59	-7.49	-3.53	-5.68	4.05
Endosome	0.72	0.61	4.40	2.98	2.62	3.71	11.84	7.50	5.20	-2.33	1.99	13.52	9.57	8.00	-2.01
Golgi	-5.96	2.05	3.24	9.22	2.48	-1.75	7.33	7.24	8.33	-1.76	-5.03	7.92	7.53	11.10	-1.20
Lipid Particles	3.00	-0.35	5.80	1.03	4.36	6.62	5.61	3.94	-3.24	-1.64	7.44	6.86	7.35	-1.05	1.16
Mitochondria	2.55	2.15	1.48	-4.26	-2.44	1.27	4.10	4.51	2.64	5.11	1.35	4.43	4.15	1.84	-0.92
Mitotic Spindle	-2.99	-2.47	-5.30	-4.71	-4.11	-1.24	-1.63	-0.77	0.45	0.68	-3.05	-3.49	-5.22	-4.72	-4.64
None	5.92	2.11	2.09	-4.52	-0.82	3.48	2.42	3.68	0.93	5.02	6.29	4.05	3.02	-3.99	-0.95
Nuclear Periphery	4.76	-8.01	-14.41	-16.31	-5.47	3.76	-21.40	-5.92	-7.54	6.11	5.63	-24.66	-15.24	-17.43	1.36
Nuclear Periphery Foci	0.05	-8.36	-4.44	-3.88	7.67	2.60	-2.80	-2.24	-2.88	-1.49	2.39	-7.57	-5.13	-6.29	2.90
Nucleolus	3.61	6.74	1.88	-1.95	-2.43	0.78	3.81	3.67	0.92	0.56	0.87	4.08	4.22	0.58	0.25
Nucleus	19.03	-7.27	-10.79	-17.47	2.12	9.43	-39.94	-5.63	-7.54	15.54	20.19	-38.66	-12.95	-18.87	20.05
Peroxisomes	1.28	2.47	-1.13	-1.60	-1.96	0.22	3.90	2.70	2.54	-2.04	0.29	4.94	-0.63	-0.75	-2.26
Vacuole	5.97	-8.88	-21.44	-22.83	-6.81	7.76	-8.36	-13.22	-14.71	-10.75	8.85	-13.81	-24.31	-26.42	-16.41
Vacuole Periphery	-3.85	2.26	5.71	5.91	2.14	2.82	9.30	7.83	6.44	-1.05	-0.93	10.48	10.04	10.05	-0.52