Standard name
Human Ortholog
Description Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.13 0.06 0.05 0 0.05 0 0 0 0.11 0.13 0.18 0.11 0.11 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0 0 0 0.12 0.07 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.92 0.96 0.81 0.88 0.86 0.8 0.72 0.75 0.7 0.72 0.86 0.83 0.7 0.81 0.82 0.88 0.95 0.92 0.92 0.81 0.7 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.09 0.08 0.2 0.32 0.36 0.39 0.44 0.45 0 0.07 0.12 0 0 0 0 0 0 0.05 0.05 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0.08 0.17 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Bud 0 0 0 0 0 1 1 0 2 1 0 0 0 0 0 0 0 1 1 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3 3 5 3 9 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 22 12 3 7 7 10 5 11 9 6 8 23 23 33 39 38 12 1 2 0 2 3 2
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 5 4 1 0 0 0 0 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 3 3 2 0 1 4 2 1 1 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 2 0 0 0 0
Mitochondria 3 5 1 4 0 8 9 30 18 14 15 1 0 2 5 10 2 1 1 0 1 2 2
Nucleus 468 216 79 43 99 166 180 176 191 121 137 183 147 125 285 292 212 482 220 76 113 125 98
Nuclear Periphery 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0 1 1 0 0 0 0 0 2
Nucleolus 6 4 1 5 9 39 72 88 100 76 85 7 13 22 12 5 8 0 0 0 6 8 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 4 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 1 6 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 3 0 0 2 4 1 2 0 2 1 1 2 1 19 16 13 8 4 1 11 31 47
Unique Cell Count 497 236 82 53 113 194 226 243 256 172 190 213 178 179 354 358 242 508 240 83 140 179 183
Labelled Cell Count 503 240 85 59 117 231 273 313 324 229 252 216 193 196 365 368 253 508 240 83 140 179 183


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.7 15.8 9.8 11.7 15.6 12.1 11.9 11.3 11.5 10.9 10.8 16.0 15.0 14.9 25.6 25.2 24.7 14.4 15.4 15.7
Std Deviation (1e-4) 4.6 4.7 3.7 3.1 3.5 4.2 4.9 5.9 5.0 6.1 4.6 7.8 5.9 7.9 10.7 11.6 8.7 5.9 4.4 4.9
Intensity Change (Log2) 0.26 0.67 0.31 0.28 0.21 0.24 0.16 0.15 0.7 0.62 0.61 1.39 1.36 1.33 0.56 0.65 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 1.9 2.3 3.2 2.0 2.0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.0 0 0 0 0 0 0 0 0 0
Nucleus -2.9 -2.1 -2.6 -3.5 -4.6 -4.3 -4.7 -4.5 -2.5 -3.1 -4.8 -3.5 -3.3 -2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 4.1 5.6 6.1 6.5 7.0 7.1 0 0 2.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.5672 9.4892 8.6641 7.431 9.7653 9.0141 9.2694 9.3584 9.1066 7.877 8.1544 8.6442 10.607 11.1768 10.2893 9.5362 9.3488 9.8973
Actin 0.0083 0 0.0155 0.0019 0 0 0.0087 0 0 0.036 0.0003 0 0.0417 0 0.0048 0.0078 0.0216 0.0092
Bud 0.0002 0 0.0002 0.0001 0 0.0001 0.0006 0.0019 0.0002 0.0001 0.0001 0 0.0004 0 0.0002 0.0001 0.0003 0.0001
Bud Neck 0.0007 0.0001 0.0002 0.0001 0.0004 0.0005 0.0014 0.0001 0 0.0002 0.0007 0.0005 0.0004 0 0.0004 0.0271 0.0003 0.0007
Bud Periphery 0.0002 0 0.0002 0.0001 0.0001 0.0003 0.0012 0.0008 0.0002 0.0002 0.0005 0.0001 0.0007 0 0.0002 0.0004 0.0005 0.0004
Bud Site 0.0017 0 0.0008 0.0005 0 0.0001 0.0014 0.002 0.0002 0.0007 0.0001 0 0.0012 0 0.0025 0.0033 0.0005 0.0001
Cell Periphery 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0006 0.0003 0.0002 0 0.0005 0 0.0002 0 0 0.0006 0.0001 0.0002
Cytoplasm 0.0081 0.0001 0.0039 0.0041 0 0 0.0037 0.0025 0.0003 0.0033 0.0001 0.0001 0.0028 0.0153 0.0002 0.0002 0.0013 0.0003
Cytoplasmic Foci 0.019 0.0001 0.0063 0.0096 0 0 0.0106 0.0001 0.0017 0.0223 0.0005 0.002 0.0143 0 0.0033 0.0079 0.0393 0.0029
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0.0003 0 0 0.0001 0 0 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0021 0 0.0005 0.0028 0 0 0.002 0.0001 0.0001 0.0003 0.0001 0 0.0016 0 0.0003 0 0.0018 0.0002
Endosome 0.0238 0.0001 0.0042 0.0246 0 0 0.0123 0 0.0002 0.0041 0.004 0.0001 0.0152 0 0.0076 0.0009 0.0189 0.0012
Golgi 0.0049 0 0.0038 0.0058 0 0 0.0029 0 0 0.0065 0.0018 0.0001 0.0057 0 0.0032 0.0017 0.0196 0.0023
Lipid Particles 0.0151 0 0.0074 0.0364 0 0 0.0183 0 0.0006 0.0105 0.0004 0.0022 0.0083 0 0.0023 0.0023 0.0061 0.0098
Mitochondria 0.0025 0.0001 0.0106 0.0049 0.0007 0.0002 0.0123 0.0002 0.001 0.0028 0.0405 0.0002 0.0025 0.0001 0.005 0.0003 0.0078 0.0046
None 0.0031 0 0.0004 0.0042 0 0 0.0032 0.0003 0.0001 0.0188 0.0001 0 0.003 0.0001 0.0001 0.0001 0.003 0.0003
Nuclear Periphery 0.0062 0.0011 0.0032 0.0108 0.0005 0.0003 0.0132 0.002 0.0019 0.0009 0.0014 0.0003 0.0265 0.0006 0.0026 0.001 0.0027 0.0014
Nucleolus 0.0103 0.0228 0.0119 0.0104 0.1228 0.0562 0.0254 0.0127 0.0126 0.0044 0.0957 0.0594 0.0126 0.0073 0.0051 0.0231 0.1156 0.0675
Nucleus 0.8656 0.974 0.9253 0.8667 0.8747 0.9406 0.8518 0.9745 0.9774 0.8595 0.8469 0.9339 0.8367 0.9764 0.9599 0.9036 0.7156 0.8936
Peroxisomes 0.0058 0 0.0007 0.0015 0 0 0.0054 0 0 0.0162 0.0006 0.0001 0.0025 0 0.0017 0.0181 0.0293 0.0008
Punctate Nuclear 0.0184 0.0012 0.0028 0.0105 0.0001 0.0001 0.0143 0.0004 0.0006 0.0125 0.0003 0.0005 0.0196 0.0002 0.0004 0.0009 0.0141 0.0013
Vacuole 0.0025 0.0002 0.0009 0.0029 0.0002 0.0011 0.0066 0.0017 0.0012 0.0001 0.0018 0.0002 0.0018 0 0.0003 0.0005 0.0006 0.0014
Vacuole Periphery 0.0013 0.0001 0.0007 0.002 0.0003 0.0003 0.0039 0.0003 0.0013 0.0001 0.0039 0.0001 0.0024 0 0.0002 0.0001 0.001 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.9898 61.9154 57.0338 66.8384 78.9624 47.74 64.7613 87.419 108.7563 68.5665
Translational Efficiency 4.8816 3.6874 3.4565 3.9311 2.7841 4.0589 2.7288 2.2455 1.855 2.385

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Chz1

Chz1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Chz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available