Standard name
Description Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.13 0.06 0.05 0 0.05 0 0 0 0.11 0.13 0.18 0.11 0.11 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0 0 0 0.12 0.07 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.92 0.96 0.81 0.88 0.86 0.8 0.72 0.75 0.7 0.72 0.86 0.83 0.7 0.81 0.82 0.88 0.95 0.92 0.92 0.81 0.7 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.09 0.08 0.2 0.32 0.36 0.39 0.44 0.45 0 0.07 0.12 0 0 0 0 0 0 0.05 0.05 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0.08 0.17 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Bud 0 0 0 0 0 1 1 0 2 1 0 0 0 0 0 0 0 1 1 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3 3 5 3 9 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 22 12 3 7 7 10 5 11 9 6 8 23 23 33 39 38 12 1 2 0 2 3 2
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 5 4 1 0 0 0 0 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 3 3 2 0 1 4 2 1 1 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 2 0 0 0 0
Mitochondria 3 5 1 4 0 8 9 30 18 14 15 1 0 2 5 10 2 1 1 0 1 2 2
Nucleus 468 216 79 43 99 166 180 176 191 121 137 183 147 125 285 292 212 482 220 76 113 125 98
Nuclear Periphery 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0 1 1 0 0 0 0 0 2
Nucleolus 6 4 1 5 9 39 72 88 100 76 85 7 13 22 12 5 8 0 0 0 6 8 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 4 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 1 6 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 3 0 0 2 4 1 2 0 2 1 1 2 1 19 16 13 8 4 1 11 31 47
Unique Cell Count 497 236 82 53 113 194 226 243 256 172 190 213 178 179 354 358 242 508 240 83 140 179 183
Labelled Cell Count 503 240 85 59 117 231 273 313 324 229 252 216 193 196 365 368 253 508 240 83 140 179 183


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.7 15.8 9.8 11.7 15.6 12.1 11.9 11.3 11.5 10.9 10.8 16.0 15.0 14.9 25.6 25.2 24.7 14.4 15.4 15.7
Std Deviation (1e-4) 4.6 4.7 3.7 3.1 3.5 4.2 4.9 5.9 5.0 6.1 4.6 7.8 5.9 7.9 10.7 11.6 8.7 5.9 4.4 4.9
Intensity Change (Log2) 0.26 0.67 0.31 0.28 0.21 0.24 0.16 0.15 0.7 0.62 0.61 1.39 1.36 1.33 0.56 0.65 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 1.9 2.3 3.2 2.0 2.0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.0 0 0 0 0 0 0 0 0 0
Nucleus -2.9 -2.1 -2.6 -3.5 -4.6 -4.3 -4.7 -4.5 -2.5 -3.1 -4.8 -3.5 -3.3 -2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 4.1 5.6 6.1 6.5 7.0 7.1 0 0 2.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Chz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available