Standard name
Description Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication; localizes to the transitional and late Golgi
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.99 0.99 0.97 0.88 0.81 0.8 0.74 0.68 0.67 0.71 0.98 0.98 0.98 0.97 0.99 0.99 0.97 0.98 0.95 0.93 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.06 0.06 0.12 0.11 0.23 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.09 0.11 0.13 0.2 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 5 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 1 0 2 1 0 2 2 0 0 0 0 0 0 1 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 5 0 2 0 3 3 9 15 20 20 20 1 1 1 4 3 6 0 0 0 0 0 0
Cytoplasm 550 446 284 255 383 326 380 467 341 349 284 338 317 372 445 211 181 172 537 432 261 236 298 406
Endoplasmic Reticulum 1 0 2 1 2 8 21 8 12 15 17 8 6 7 6 3 6 3 0 1 3 1 2 7
Endosome 1 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 0 0
Mitochondria 1 0 1 0 2 18 28 38 56 55 98 81 0 0 0 1 1 1 0 0 0 1 2 2
Nucleus 0 1 0 1 2 3 10 18 13 8 11 15 3 0 4 2 0 0 1 1 1 1 1 3
Nuclear Periphery 0 0 0 0 0 0 1 0 4 2 3 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 0 0 11 12 22 44 63 60 104 37 33 0 1 3 3 3 0 6 0 0 3 4 8
Unique Cell Count 558 447 286 258 396 371 471 585 461 511 425 473 322 378 454 217 183 173 554 440 275 253 317 442
Labelled Cell Count 562 453 287 270 401 382 488 610 502 559 473 501 327 381 459 224 194 182 554 440 275 253 317 442


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.9 17.5 13.8 13.0 12.2 10.9 10.3 10.4 10.1 9.8 9.6 9.9 15.3 14.3 14.4 21.4 21.6 21.0 16.5 17.3 16.8
Std Deviation (1e-4) 2.3 2.6 2.3 2.1 1.9 1.9 2.0 2.0 2.0 2.0 2.0 2.2 2.2 2.1 2.0 4.6 5.2 5.0 2.4 2.7 3.0
Intensity Change (Log2) – – – -0.08 -0.18 -0.34 -0.42 -0.4 -0.45 -0.49 -0.53 -0.48 0.15 0.06 0.07 0.64 0.65 0.61 0.26 0.33 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3.1 3.4 3.7 3.5 0 0 0 0 0 0
Cytoplasm -0.6 -2.3 -5.6 -7.6 -7.8 -9.1 -10.4 -10.6 -9.7 -1.0 -1.0 -1.4 -1.8 -0.5 0.2
Endoplasmic Reticulum 0 0 0 2.9 0 1.9 2.1 2.7 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 3.9 4.1 5.9 5.5 8.6 7.2 0 0 0 0 0 0
Nucleus 0 0 0 0 3.0 0 0 0 3.0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.5 3.0 4.2 5.3 5.8 6.4 8.2 5.1 4.6 0 0 0 0 0 0