Standard name | |
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Description | Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication; localizes to the transitional and late Golgi |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 5 | 0 | 2 | 0 | 3 | 3 | 9 | 15 | 20 | 20 | 20 | 1 | 1 | 1 | 4 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 550 | 446 | 284 | 255 | 383 | 326 | 380 | 467 | 341 | 349 | 284 | 338 | 317 | 372 | 445 | 211 | 181 | 172 | 537 | 432 | 261 | 236 | 298 | 406 |
Endoplasmic Reticulum | 1 | 0 | 2 | 1 | 2 | 8 | 21 | 8 | 12 | 15 | 17 | 8 | 6 | 7 | 6 | 3 | 6 | 3 | 0 | 1 | 3 | 1 | 2 | 7 |
Endosome | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 0 |
Mitochondria | 1 | 0 | 1 | 0 | 2 | 18 | 28 | 38 | 56 | 55 | 98 | 81 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 2 |
Nucleus | 0 | 1 | 0 | 1 | 2 | 3 | 10 | 18 | 13 | 8 | 11 | 15 | 3 | 0 | 4 | 2 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 0 | 0 | 11 | 12 | 22 | 44 | 63 | 60 | 104 | 37 | 33 | 0 | 1 | 3 | 3 | 3 | 0 | 6 | 0 | 0 | 3 | 4 | 8 |
Unique Cell Count | 558 | 447 | 286 | 258 | 396 | 371 | 471 | 585 | 461 | 511 | 425 | 473 | 322 | 378 | 454 | 217 | 183 | 173 | 554 | 440 | 275 | 253 | 317 | 442 |
Labelled Cell Count | 562 | 453 | 287 | 270 | 401 | 382 | 488 | 610 | 502 | 559 | 473 | 501 | 327 | 381 | 459 | 224 | 194 | 182 | 554 | 440 | 275 | 253 | 317 | 442 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.9 | 17.5 | 13.8 | 13.0 | 12.2 | 10.9 | 10.3 | 10.4 | 10.1 | 9.8 | 9.6 | 9.9 | 15.3 | 14.3 | 14.4 | 21.4 | 21.6 | 21.0 | 16.5 | 17.3 | 16.8 |
Std Deviation (1e-4) | 2.3 | 2.6 | 2.3 | 2.1 | 1.9 | 1.9 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.2 | 2.2 | 2.1 | 2.0 | 4.6 | 5.2 | 5.0 | 2.4 | 2.7 | 3.0 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.18 | -0.34 | -0.42 | -0.4 | -0.45 | -0.49 | -0.53 | -0.48 | 0.15 | 0.06 | 0.07 | 0.64 | 0.65 | 0.61 | 0.26 | 0.33 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 3.1 | 3.4 | 3.7 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -2.3 | -5.6 | -7.6 | -7.8 | -9.1 | -10.4 | -10.6 | -9.7 | -1.0 | -1.0 | -1.4 | -1.8 | -0.5 | 0.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.9 | 0 | 1.9 | 2.1 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.4 | 3.9 | 4.1 | 5.9 | 5.5 | 8.6 | 7.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.5 | 3.0 | 4.2 | 5.3 | 5.8 | 6.4 | 8.2 | 5.1 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 |