Standard name
Human Ortholog
Description Endoplasmic reticulum membrane protein; may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0.05 0.05 0.06 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.91 0.92 0.86 0.76 0.74 0.71 0.69 0.79 0.83 0.8 0.81 0.81 0.73 0.83 0.92 0.83 0.91 0.27 0.31 0.37 0.25 0.23 0.23
Endoplasmic Reticulum 0.05 0 0.06 0.16 0.19 0.17 0.11 0.09 0 0 0.07 0.18 0.24 0.13 0.08 0 0 0.23 0.26 0.11 0.23 0.36 0.38
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0.09 0.11 0 0 0 0 0.06 0.05 0.05 0.07 0 0 0 0 0 0.05 0.05 0.05 0.12 0.14 0.08 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0.05 0.11 0.05 0.06 0.06 0.06 0 0 0 0 0.09 0 0.4 0.32 0.3 0.27 0.21 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 17 13 29 30 20 13 14 9 6 25 15 2 3 1 1 0 1 2 13 15
Bud 1 3 9 16 21 33 38 35 35 43 27 7 6 5 0 0 0 1 0 1 2 6 9
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 0 3 2 6 12
Bud Site 0 0 0 1 0 0 2 1 2 1 1 0 0 0 0 0 0
Cell Periphery 2 1 4 15 14 15 12 12 11 23 14 6 17 38 2 4 0 0 0 0 0 0 3
Cytoplasm 353 134 243 344 301 501 494 435 475 450 527 350 446 585 47 54 39 115 10 61 39 100 117
Endoplasmic Reticulum 19 2 18 73 78 120 79 48 19 24 44 76 143 92 4 2 1 95 8 18 37 157 191
Endosome 2 0 4 18 7 26 28 4 11 10 11 7 32 28 0 3 1 6 0 3 5 13 17
Golgi 0 0 0 0 0 0 2 0 0 0 0 0 2 2 0 0 2 2 0 1 1 4 1
Mitochondria 4 13 31 4 16 22 19 34 28 29 46 1 3 2 0 1 2 21 1 19 23 34 26
Nucleus 0 0 0 1 1 4 6 1 4 4 1 0 0 1 0 0 0 0 0 0 1 1 0
Nuclear Periphery 0 0 1 1 0 4 4 0 3 1 5 0 0 1 0 0 0 1 0 0 0 1 1
Nucleolus 0 1 0 1 0 2 6 2 0 2 1 0 1 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 2 0 0 2 0 0 1 0 1 1 4 0 0 0 0 0 1 0 1 1
Vac/Vac Membrane 12 4 1 21 10 32 76 25 33 31 40 11 5 5 2 6 0 167 11 50 42 92 99
Unique Cell Count 386 145 282 452 407 709 718 553 571 561 648 433 608 708 51 65 43 422 35 166 160 438 503
Labelled Cell Count 395 159 312 514 462 788 798 617 634 633 726 465 681 779 57 73 47 422 35 166 160 438 503


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 3.6 4.9 6.6 6.6 6.9 7.1 6.5 6.9 6.6 6.3 6.8 7.1 6.7 9.9 9.1 9.2 5.8 6.4 7.0
Std Deviation (1e-4) 0.7 0.8 1.2 1.4 1.2 1.4 1.3 1.4 1.6 1.5 1.4 1.1 1.2 1.3 1.9 1.5 1.9 1.7 1.2 1.4
Intensity Change (Log2) 0.42 0.86 0.85 0.92 0.95 0.83 0.91 0.85 0.79 0.91 0.97 0.88 1.44 1.31 1.34 0.67 0.81 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 2.0 2.1 0 0 0 0 0 2.0 0 0 0 0
Bud 0 0 1.6 1.4 1.7 2.0 1.9 2.4 1.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0 0
Cytoplasm -1.9 -4.3 -4.7 -5.5 -5.8 -3.8 -2.8 -3.5 -3.2 -3.3 -4.9 -3.0 -0.1 -2.0 -0.4
Endoplasmic Reticulum 0 4.7 5.2 4.9 3.6 3.0 0 1.7 2.5 4.9 6.1 4.1 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 2.8 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 -2.3 -3.2 -3.7 -1.2 -1.9 -1.7 -0.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.0 0 1.0 3.0 0.9 1.5 1.4 1.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9698 1.7476 1.3387 0.9693 0.5554 1.7316 1.0831 2.0083 1.5139 1.3656 1.0274 1.5243 3.4447 4.9345 3.7871 3.8978 4.1276 3.8682
Actin 0.0125 0.0003 0.0044 0.0192 0 0.0002 0.0286 0.0097 0.0274 0.0317 0.0033 0.001 0.0251 0.0007 0.0026 0.02 0.0012 0.01
Bud 0.0004 0.0008 0.0002 0.0003 0.0002 0 0.0008 0.0034 0.0043 0.0029 0.0008 0.0001 0.0002 0.0005 0.0004 0.0002 0.0001 0.0012
Bud Neck 0.0089 0.0008 0.0027 0.0017 0.001 0.0008 0.0026 0.0005 0.0063 0.0019 0.0004 0.0009 0.0011 0.0002 0.0003 0.0002 0.0026 0.0024
Bud Periphery 0.0007 0.0014 0.0002 0.0005 0.0001 0 0.0011 0.0024 0.0022 0.0037 0.001 0.0002 0.0003 0.0003 0.0004 0.0002 0.0001 0.0012
Bud Site 0.0036 0.0043 0.0012 0.0045 0.0011 0.0001 0.0073 0.0234 0.0164 0.0096 0.0005 0.0003 0.0007 0.0032 0.0008 0.0002 0.0003 0.0016
Cell Periphery 0.0013 0.0012 0.0006 0.0009 0.0003 0.0001 0.0009 0.0011 0.0008 0.0009 0.0002 0.0004 0.0008 0.0007 0.0003 0.0002 0.0004 0.0004
Cytoplasm 0.3797 0.6229 0.4963 0.3346 0.4408 0.5183 0.3458 0.5303 0.4192 0.4352 0.4206 0.5193 0.4385 0.5675 0.5253 0.3718 0.5076 0.5383
Cytoplasmic Foci 0.0351 0.0188 0.041 0.0624 0.035 0.0364 0.0566 0.0352 0.0758 0.0402 0.0408 0.0515 0.0399 0.0363 0.0384 0.0216 0.0265 0.0224
Eisosomes 0.002 0 0.0001 0.0003 0 0 0.0002 0.0001 0.0002 0.0006 0 0 0.0003 0 0 0.0001 0 0.0001
Endoplasmic Reticulum 0.1611 0.1551 0.0862 0.0848 0.0092 0.0904 0.2086 0.1615 0.1314 0.1141 0.1745 0.1183 0.1928 0.1376 0.1449 0.1674 0.111 0.1832
Endosome 0.1009 0.0781 0.1137 0.2216 0.3013 0.1075 0.1303 0.099 0.1744 0.1676 0.2408 0.1285 0.1038 0.1 0.1455 0.15 0.1693 0.0974
Golgi 0.0132 0.002 0.0062 0.0259 0.0015 0.0022 0.0183 0.0048 0.0142 0.0223 0.0313 0.0101 0.0068 0.0034 0.0068 0.0075 0.0079 0.0067
Lipid Particles 0.0168 0.009 0.0158 0.0116 0.0014 0.0068 0.0379 0.0093 0.0101 0.0166 0.0042 0.0266 0.0134 0.0056 0.0048 0.0044 0.03 0.0022
Mitochondria 0.0054 0.0013 0.0016 0.012 0.0004 0.0006 0.0098 0.0012 0.0051 0.0409 0.0228 0.0133 0.0027 0.0008 0.0015 0.0042 0.0013 0.0016
None 0.1648 0.0512 0.174 0.0934 0.0166 0.1685 0.0843 0.0727 0.0561 0.0466 0.0117 0.0758 0.0995 0.0666 0.0603 0.183 0.0495 0.0575
Nuclear Periphery 0.0283 0.013 0.0079 0.0324 0.005 0.012 0.0159 0.0099 0.0089 0.0058 0.0053 0.0063 0.0168 0.0115 0.0115 0.0137 0.0259 0.0098
Nucleolus 0.004 0.0017 0.0002 0.0004 0.0004 0.0001 0.0006 0.0006 0.0009 0.0014 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0056 0.0037 0.002 0.004 0.0027 0.0022 0.0095 0.0032 0.0046 0.0021 0.0023 0.0028 0.003 0.0032 0.0028 0.0024 0.0082 0.0033
Peroxisomes 0.0024 0.0002 0.0094 0.0187 0.0001 0.0003 0.0136 0.0005 0.009 0.0163 0.0003 0.006 0.0028 0.0005 0.0007 0.004 0.0001 0.0005
Punctate Nuclear 0.0191 0.0008 0.0027 0.0052 0.0003 0.0009 0.0023 0.003 0.0082 0.0017 0.0007 0.001 0.0064 0.001 0.0007 0.0009 0.0006 0.0008
Vacuole 0.0311 0.0304 0.0292 0.0564 0.1746 0.0476 0.0217 0.0257 0.0206 0.0326 0.0318 0.031 0.0413 0.0568 0.047 0.041 0.0519 0.0548
Vacuole Periphery 0.003 0.0029 0.0046 0.009 0.0081 0.0049 0.0033 0.0024 0.0038 0.0052 0.0063 0.0065 0.0034 0.0034 0.0046 0.007 0.0054 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.072 33.0999 52.3979 51.8942 30.4045 54.2762 46.2964 54.9645 56.989 50.1321
Translational Efficiency 1.4366 1.6038 1.21 1.1017 1.758 1.575 1.5569 1.8383 1.4242 1.5961

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency